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P13528

- UNC86_CAEEL

UniProt

P13528 - UNC86_CAEEL

Protein

Transcription factor unc-86

Gene

unc-86

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 135 (01 Oct 2014)
      Sequence version 1 (01 Jan 1990)
      Previous versions | rss
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    Functioni

    Transcription factor required for correct cell fate determination and differentiation in diverse neuronal cell lineages where it plays a role in specifying the fate of daughter cells during cell divisions. Involved in sensory neuron production and function. Binds both alone and with mec-3 to the mec-3 promoter to initiate and maintain mec-3 expression which is required for sensory neuron differentiation. In addition, binds both alone and with mec-3 to the promoters of mec-4 and mec-7 which act to regulate sensory neuron function. Involved in determining the identity of the serotonergic NSM neurons and the cholinergic IL2 sensory and URA motor neurons. Promotes expression of the cfi-1 transcription factor in the URA and IL2 neurons which in turn activates normal URA and IL2 gene expression. Regulates expression of a number of genes in NSM neurons including bas-1, cat-1, dop-3, mgl-3, nlp-13, scd-2 and ptps-1. In the IL2 neurons, required for expression of cho-1, gcy-19, klp-6, lag-2, unc-5 and unc-17. Promotes expression of pkd-2 in the male-specific CEM head neurons. Required for dauer-specific branching of IL2Q neurons and nictation behavior. Controls both the timing and direction of axon outgrowth in HSN neurons. Plays a role in serotonin production by regulating expression of the tryptophan hydrolase tph-1 which catalyzes serotonin synthesis, in the AIM, NSM, HSN and RIH neurons. Involved in regulation of lin-11 expression in the AIZ interneurons, the major interneurons of the olfactory pathway, and is required for odortaxis behavior. According to PubMed:24353061, required for correct localization of unc-40.11 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi363 – 42260HomeoboxPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. RNA polymerase II regulatory region sequence-specific DNA binding Source: WormBase
    2. sequence-specific DNA binding Source: WormBase
    3. sequence-specific DNA binding RNA polymerase II transcription factor activity Source: WormBase
    4. transcription factor binding Source: WormBase

    GO - Biological processi

    1. mechanosensory behavior Source: WormBase
    2. neuron development Source: WormBase
    3. positive regulation of transcription from RNA polymerase II promoter Source: WormBase
    4. response to mechanical stimulus Source: WormBase
    5. transcription from RNA polymerase II promoter Source: GOC

    Keywords - Biological processi

    Neurogenesis, Olfaction, Sensory transduction, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    SignaLinkiP13528.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcription factor unc-86
    Alternative name(s):
    Uncoordinated protein 86
    Gene namesi
    Name:unc-86
    ORF Names:C30A5.7
    OrganismiCaenorhabditis elegans
    Taxonomic identifieri6239 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    ProteomesiUP000001940: Chromosome III

    Organism-specific databases

    WormBaseiC30A5.7a; CE45098; WBGene00006818; unc-86.
    C30A5.7b; CE45067; WBGene00006818; unc-86.

    Subcellular locationi

    Nucleus 1 PublicationPROSITE-ProRule annotation

    GO - Cellular componenti

    1. nucleus Source: WormBase

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    In a number of neuronal cell lineages, one of the two daughter cells of a division fails to assume its normal fate, retaining instead the fate of its parent cell. Altered gene expression in a number of neuron types including reduced cat-1 expression in AIM, NSM, HSN and RIH neurons, loss of cat-4 expression in HSN neurons, loss of expression of dop-3 and mgl-3 and reduced expression of bas-1, nlp-13, ptpa-1 and scd-2 in NSM neurons, loss of expression of gcy-19, klp-6, lag-2 and unc-17 and reduced expression of cho-1 and unc-5 in IL2 neurons, and loss of tph-1 expression in AIM, NSM, HSN and RIH neurons which leads to defects in serotonin production. Defective motility along with defects in IL2Q dendritic arborization and nictation. Abnormal neurite outgrowth in NSM neurons. Defective response to odor attractants but odor repellants are avoided normally. Penetrant ventral axon growth defects in HSN motor neurons with extension of processes in L1, three stages earlier than wild-type. Incorrect unc-40 localization. Defective egg-laying characterized by retention of substantially more eggs in the uterus of second-day animals than the wild-type.6 Publications

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi270 – 2701P → S in u168; defective mechanosensation, lack of interaction with mec-3 and lack of maintained mec-3 expression. No egg-laying or Q neuroblast lineage defects. 1 Publication
    Mutagenesisi320 – 3201L → F in u5; defective mechanosensation, lack of interaction with mec-3 and lack of maintained mec-3 expression. No egg-laying or Q neuroblast lineage defects. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 467467Transcription factor unc-86PRO_0000100780Add
    BLAST

    Proteomic databases

    PaxDbiP13528.
    PRIDEiP13528.

    Expressioni

    Tissue specificityi

    Specific to neurons and neuroblasts. Expressed in CEM head neurons and in IL2, URA, URB, URX and URY neurons. Not expressed in olfactory sensory neurons but expressed in AIZ interneurons.3 Publications

    Developmental stagei

    First appears during embryogenesis and persists throughout adulthood.1 Publication

    Interactioni

    Subunit structurei

    Interacts with mec-3; the heterooligomer binds to the promoters of mec-3, mec-4 and mec-7.2 Publications

    Protein-protein interaction databases

    IntActiP13528. 1 interaction.
    STRINGi6239.C30A5.7b.

    Structurei

    3D structure databases

    ProteinModelPortaliP13528.
    SMRiP13528. Positions 269-420.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini265 – 34278POU-specificPROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi145 – 15410POU-IV box

    Sequence similaritiesi

    Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation
    Contains 1 POU-specific domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Homeobox

    Phylogenomic databases

    eggNOGiNOG289030.
    HOGENOMiHOG000116305.
    InParanoidiP13528.
    OrthoDBiEOG7RV9G7.
    PhylomeDBiP13528.

    Family and domain databases

    Gene3Di1.10.10.60. 1 hit.
    1.10.260.40. 1 hit.
    InterProiIPR017970. Homeobox_CS.
    IPR001356. Homeobox_dom.
    IPR009057. Homeodomain-like.
    IPR010982. Lambda_DNA-bd_dom.
    IPR013847. POU.
    IPR015584. POU4-related.
    IPR000327. POU_specific.
    [Graphical view]
    PANTHERiPTHR11636:SF10. PTHR11636:SF10. 1 hit.
    PfamiPF00046. Homeobox. 1 hit.
    PF00157. Pou. 1 hit.
    [Graphical view]
    PRINTSiPR00028. POUDOMAIN.
    SMARTiSM00389. HOX. 1 hit.
    SM00352. POU. 1 hit.
    [Graphical view]
    SUPFAMiSSF46689. SSF46689. 1 hit.
    SSF47413. SSF47413. 1 hit.
    PROSITEiPS00027. HOMEOBOX_1. 1 hit.
    PS50071. HOMEOBOX_2. 1 hit.
    PS00035. POU_1. 1 hit.
    PS00465. POU_2. 1 hit.
    PS51179. POU_3. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform b (identifier: P13528-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MEKAHRFRLP FCSFFPVPLL VSVLIFHHSA PFLIQLFFPS PLFNPLLRPS    50
    KISRGSENGA CTSHSTLQRT RKIIQWELPK RGGDQDIGDP RPFRIHLSPP 100
    SFKVPLFSTD MQNTAPVPTT TTASKMQPFN NSLFGSFDDP ILNARAAQVA 150
    LADIDVKNVP QLTNPLMRPH DMFSYSNYFS GIHDTSAATN IYQGLPSSSE 200
    PFDASVVVPT SSDDQMTPLQ QVMAMQQSYG APPPFQYNMT HPFSTTSIAS 250
    SNNLARYPIA PPTSDMDTDP RQLETFAEHF KQRRIKLGVT QADVGKALAH 300
    LKMPGVGSLS QSTICRFESL TLSHNNMVAL KPILHSWLEK AEEAMKQKDT 350
    IGDINGILPN TDKKRKRTSI AAPEKRELEQ FFKQQPRPSG ERIASIADRL 400
    DLKKNVVRVW FCNQRQKQKR DFRSQFRARS AAAVMGPRVM PVLNGNNSNN 450
    NLKQGQTTYN GLPGFFD 467
    Length:467
    Mass (Da):52,315
    Last modified:January 1, 1990 - v1
    Checksum:i86C3507584C91AC2
    GO
    Isoform a (identifier: P13528-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         129-134: FNNSLF → DFILFI
         135-172: Missing.

    Show »
    Length:429
    Mass (Da):48,214
    Checksum:i4389449360DC5FC9
    GO

    Sequence cautioni

    The sequence CCD62655.1 differs from that shown. Reason: Erroneous gene model prediction.
    The sequence CCD62656.1 differs from that shown. Reason: Erroneous gene model prediction.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei129 – 1346FNNSLF → DFILFI in isoform a. CuratedVSP_002343
    Alternative sequencei135 – 17238Missing in isoform a. CuratedVSP_002344Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M22363 Genomic DNA. Translation: AAA28158.1.
    M22363 Genomic DNA. Translation: AAA28159.1.
    FO080290 Genomic DNA. Translation: CCD62655.1. Sequence problems.
    FO080290 Genomic DNA. Translation: CCD62656.1. Sequence problems.
    PIRiA30042.
    S44778.
    RefSeqiNP_001021191.2. NM_001026020.4.
    NP_498795.2. NM_066394.2.
    UniGeneiCel.24958.

    Genome annotation databases

    GeneIDi176157.
    KEGGicel:CELE_C30A5.7.
    UCSCiC30A5.7b. c. elegans. [P13528-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M22363 Genomic DNA. Translation: AAA28158.1 .
    M22363 Genomic DNA. Translation: AAA28159.1 .
    FO080290 Genomic DNA. Translation: CCD62655.1 . Sequence problems.
    FO080290 Genomic DNA. Translation: CCD62656.1 . Sequence problems.
    PIRi A30042.
    S44778.
    RefSeqi NP_001021191.2. NM_001026020.4.
    NP_498795.2. NM_066394.2.
    UniGenei Cel.24958.

    3D structure databases

    ProteinModelPortali P13528.
    SMRi P13528. Positions 269-420.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P13528. 1 interaction.
    STRINGi 6239.C30A5.7b.

    Proteomic databases

    PaxDbi P13528.
    PRIDEi P13528.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 176157.
    KEGGi cel:CELE_C30A5.7.
    UCSCi C30A5.7b. c. elegans. [P13528-1 ]

    Organism-specific databases

    CTDi 176157.
    WormBasei C30A5.7a ; CE45098 ; WBGene00006818 ; unc-86.
    C30A5.7b ; CE45067 ; WBGene00006818 ; unc-86.

    Phylogenomic databases

    eggNOGi NOG289030.
    HOGENOMi HOG000116305.
    InParanoidi P13528.
    OrthoDBi EOG7RV9G7.
    PhylomeDBi P13528.

    Enzyme and pathway databases

    SignaLinki P13528.

    Miscellaneous databases

    NextBioi 891370.

    Family and domain databases

    Gene3Di 1.10.10.60. 1 hit.
    1.10.260.40. 1 hit.
    InterProi IPR017970. Homeobox_CS.
    IPR001356. Homeobox_dom.
    IPR009057. Homeodomain-like.
    IPR010982. Lambda_DNA-bd_dom.
    IPR013847. POU.
    IPR015584. POU4-related.
    IPR000327. POU_specific.
    [Graphical view ]
    PANTHERi PTHR11636:SF10. PTHR11636:SF10. 1 hit.
    Pfami PF00046. Homeobox. 1 hit.
    PF00157. Pou. 1 hit.
    [Graphical view ]
    PRINTSi PR00028. POUDOMAIN.
    SMARTi SM00389. HOX. 1 hit.
    SM00352. POU. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46689. SSF46689. 1 hit.
    SSF47413. SSF47413. 1 hit.
    PROSITEi PS00027. HOMEOBOX_1. 1 hit.
    PS50071. HOMEOBOX_2. 1 hit.
    PS00035. POU_1. 1 hit.
    PS00465. POU_2. 1 hit.
    PS51179. POU_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The C. elegans cell lineage and differentiation gene unc-86 encodes a protein with a homeodomain and extended similarity to transcription factors."
      Finney M., Ruvkun G., Horvitz H.R.
      Cell 55:757-769(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS A AND B).
    2. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING.
      Strain: Bristol N2.
    3. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
      The C. elegans sequencing consortium
      Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Bristol N2.
    4. "Mutations that lead to reiterations in the cell lineages of C. elegans."
      Chalfie M., Horvitz H.R., Sulston J.E.
      Cell 24:59-69(1981) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.
      Strain: Bristol N2.
    5. "The unc-86 gene product couples cell lineage and cell identity in C. elegans."
      Finney M., Ruvkun G.
      Cell 63:895-905(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    6. "Regulation of the mec-3 gene by the C.elegans homeoproteins UNC-86 and MEC-3."
      Xue D., Finney M., Ruvkun G., Chalfie M.
      EMBO J. 11:4969-4979(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Regulation of touch receptor differentiation by the Caenorhabditis elegans mec-3 and unc-86 genes."
      Duggan A., Ma C., Chalfie M.
      Development 125:4107-4119(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT.
      Strain: Bristol N2.
    8. "Protein interaction surface of the POU transcription factor UNC-86 selectively used in touch neurons."
      Rohrig S., Rockelein I., Donhauser R., Baumeister R.
      EMBO J. 19:3694-3703(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT, DISRUPTION PHENOTYPE, MUTAGENESIS OF PRO-270 AND LEU-320.
    9. "The C. elegans POU-domain transcription factor UNC-86 regulates the tph-1 tryptophan hydroxylase gene and neurite outgrowth in specific serotonergic neurons."
      Sze J.Y., Zhang S., Li J., Ruvkun G.
      Development 129:3901-3911(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.
      Strain: Bristol N2.
    10. "Control of neuronal subtype identity by the C. elegans ARID protein CFI-1."
      Shaham S., Bargmann C.I.
      Genes Dev. 16:972-983(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY.
      Strain: Bristol N2.
    11. "Activity of the Caenorhabditis elegans UNC-86 POU transcription factor modulates olfactory sensitivity."
      Sze J.Y., Ruvkun G.
      Proc. Natl. Acad. Sci. U.S.A. 100:9560-9565(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
    12. "The POU transcription factor UNC-86 controls the timing and ventral guidance of Caenorhabditis elegans axon growth."
      Olsson-Carter K., Slack F.J.
      Dev. Dyn. 240:1815-1825(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.
      Strain: Bristol N2.
    13. "Dauer-specific dendrite arborization in C. elegans is regulated by KPC-1/Furin."
      Schroeder N.E., Androwski R.J., Rashid A., Lee H., Lee J., Barr M.M.
      Curr. Biol. 23:1527-1535(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
      Strain: Bristol N2.
    14. "The LIM and POU homeobox genes ttx-3 and unc-86 act as terminal selectors in distinct cholinergic and serotonergic neuron types."
      Zhang F., Bhattacharya A., Nelson J.C., Abe N., Gordon P., Lloret-Fernandez C., Maicas M., Flames N., Mann R.S., Colon-Ramos D.A., Hobert O.
      Development 141:422-435(2014) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DNA-BINDING, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiUNC86_CAEEL
    AccessioniPrimary (citable) accession number: P13528
    Secondary accession number(s): D7SFI2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: January 1, 1990
    Last modified: October 1, 2014
    This is version 135 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3