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P13528

- UNC86_CAEEL

UniProt

P13528 - UNC86_CAEEL

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Protein

Transcription factor unc-86

Gene
unc-86, C30A5.7
Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Transcription factor required for correct cell fate determination and differentiation in diverse neuronal cell lineages where it plays a role in specifying the fate of daughter cells during cell divisions. Involved in sensory neuron production and function. Binds both alone and with mec-3 to the mec-3 promoter to initiate and maintain mec-3 expression which is required for sensory neuron differentiation. In addition, binds both alone and with mec-3 to the promoters of mec-4 and mec-7 which act to regulate sensory neuron function. Involved in determining the identity of the serotonergic NSM neurons and the cholinergic IL2 sensory and URA motor neurons. Promotes expression of the cfi-1 transcription factor in the URA and IL2 neurons which in turn activates normal URA and IL2 gene expression. Regulates expression of a number of genes in NSM neurons including bas-1, cat-1, dop-3, mgl-3, nlp-13, scd-2 and ptps-1. In the IL2 neurons, required for expression of cho-1, gcy-19, klp-6, lag-2, unc-5 and unc-17. Promotes expression of pkd-2 in the male-specific CEM head neurons. Required for dauer-specific branching of IL2Q neurons and nictation behavior. Controls both the timing and direction of axon outgrowth in HSN neurons. Plays a role in serotonin production by regulating expression of the tryptophan hydrolase tph-1 which catalyzes serotonin synthesis, in the AIM, NSM, HSN and RIH neurons. Involved in regulation of lin-11 expression in the AIZ interneurons, the major interneurons of the olfactory pathway, and is required for odortaxis behavior. According to 1 Publication, required for correct localization of unc-40.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi363 – 42260HomeoboxAdd
BLAST

GO - Molecular functioni

  1. RNA polymerase II regulatory region sequence-specific DNA binding Source: WormBase
  2. sequence-specific DNA binding Source: WormBase
  3. sequence-specific DNA binding RNA polymerase II transcription factor activity Source: WormBase
  4. transcription factor binding Source: WormBase

GO - Biological processi

  1. mechanosensory behavior Source: WormBase
  2. neuron development Source: WormBase
  3. positive regulation of transcription from RNA polymerase II promoter Source: WormBase
  4. response to mechanical stimulus Source: WormBase
  5. transcription from RNA polymerase II promoter Source: GOC
Complete GO annotation...

Keywords - Biological processi

Neurogenesis, Olfaction, Sensory transduction, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

SignaLinkiP13528.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor unc-86
Alternative name(s):
Uncoordinated protein 86
Gene namesi
Name:unc-86
ORF Names:C30A5.7
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome III

Organism-specific databases

WormBaseiC30A5.7a; CE45098; WBGene00006818; unc-86.
C30A5.7b; CE45067; WBGene00006818; unc-86.

Subcellular locationi

GO - Cellular componenti

  1. nucleus Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

In a number of neuronal cell lineages, one of the two daughter cells of a division fails to assume its normal fate, retaining instead the fate of its parent cell. Altered gene expression in a number of neuron types including reduced cat-1 expression in AIM, NSM, HSN and RIH neurons, loss of cat-4 expression in HSN neurons, loss of expression of dop-3 and mgl-3 and reduced expression of bas-1, nlp-13, ptpa-1 and scd-2 in NSM neurons, loss of expression of gcy-19, klp-6, lag-2 and unc-17 and reduced expression of cho-1 and unc-5 in IL2 neurons, and loss of tph-1 expression in AIM, NSM, HSN and RIH neurons which leads to defects in serotonin production. Defective motility along with defects in IL2Q dendritic arborization and nictation. Abnormal neurite outgrowth in NSM neurons. Defective response to odor attractants but odor repellants are avoided normally. Penetrant ventral axon growth defects in HSN motor neurons with extension of processes in L1, three stages earlier than wild-type. Incorrect unc-40 localization. Defective egg-laying characterized by retention of substantially more eggs in the uterus of second-day animals than the wild-type.

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi270 – 2701P → S in u168; defective mechanosensation, lack of interaction with mec-3 and lack of maintained mec-3 expression. No egg-laying or Q neuroblast lineage defects.
Mutagenesisi320 – 3201L → F in u5; defective mechanosensation, lack of interaction with mec-3 and lack of maintained mec-3 expression. No egg-laying or Q neuroblast lineage defects.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 467467Transcription factor unc-86PRO_0000100780Add
BLAST

Proteomic databases

PaxDbiP13528.
PRIDEiP13528.

Expressioni

Tissue specificityi

Specific to neurons and neuroblasts. Expressed in CEM head neurons and in IL2, URA, URB, URX and URY neurons. Not expressed in olfactory sensory neurons but expressed in AIZ interneurons.

Developmental stagei

First appears during embryogenesis and persists throughout adulthood.

Interactioni

Subunit structurei

Interacts with mec-3; the heterooligomer binds to the promoters of mec-3, mec-4 and mec-7.

Protein-protein interaction databases

IntActiP13528. 1 interaction.
STRINGi6239.C30A5.7b.

Structurei

3D structure databases

ProteinModelPortaliP13528.
SMRiP13528. Positions 269-420.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini265 – 34278POU-specificAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi145 – 15410POU-IV box

Sequence similaritiesi

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiNOG289030.
HOGENOMiHOG000116305.
InParanoidiP13528.
OrthoDBiEOG7RV9G7.
PhylomeDBiP13528.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR015584. POU4-related.
IPR000327. POU_specific.
[Graphical view]
PANTHERiPTHR11636:SF10. PTHR11636:SF10. 1 hit.
PfamiPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PRINTSiPR00028. POUDOMAIN.
SMARTiSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform b (identifier: P13528-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MEKAHRFRLP FCSFFPVPLL VSVLIFHHSA PFLIQLFFPS PLFNPLLRPS    50
KISRGSENGA CTSHSTLQRT RKIIQWELPK RGGDQDIGDP RPFRIHLSPP 100
SFKVPLFSTD MQNTAPVPTT TTASKMQPFN NSLFGSFDDP ILNARAAQVA 150
LADIDVKNVP QLTNPLMRPH DMFSYSNYFS GIHDTSAATN IYQGLPSSSE 200
PFDASVVVPT SSDDQMTPLQ QVMAMQQSYG APPPFQYNMT HPFSTTSIAS 250
SNNLARYPIA PPTSDMDTDP RQLETFAEHF KQRRIKLGVT QADVGKALAH 300
LKMPGVGSLS QSTICRFESL TLSHNNMVAL KPILHSWLEK AEEAMKQKDT 350
IGDINGILPN TDKKRKRTSI AAPEKRELEQ FFKQQPRPSG ERIASIADRL 400
DLKKNVVRVW FCNQRQKQKR DFRSQFRARS AAAVMGPRVM PVLNGNNSNN 450
NLKQGQTTYN GLPGFFD 467
Length:467
Mass (Da):52,315
Last modified:January 1, 1990 - v1
Checksum:i86C3507584C91AC2
GO
Isoform a (identifier: P13528-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     129-134: FNNSLF → DFILFI
     135-172: Missing.

Show »
Length:429
Mass (Da):48,214
Checksum:i4389449360DC5FC9
GO

Sequence cautioni

The sequence CCD62655.1 differs from that shown. Reason: Erroneous gene model prediction.
The sequence CCD62656.1 differs from that shown. Reason: Erroneous gene model prediction.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei129 – 1346FNNSLF → DFILFI in isoform a. VSP_002343
Alternative sequencei135 – 17238Missing in isoform a. VSP_002344Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M22363 Genomic DNA. Translation: AAA28158.1.
M22363 Genomic DNA. Translation: AAA28159.1.
FO080290 Genomic DNA. Translation: CCD62655.1. Sequence problems.
FO080290 Genomic DNA. Translation: CCD62656.1. Sequence problems.
PIRiA30042.
S44778.
RefSeqiNP_001021191.2. NM_001026020.4.
NP_498795.2. NM_066394.2.
UniGeneiCel.24958.

Genome annotation databases

GeneIDi176157.
KEGGicel:CELE_C30A5.7.
UCSCiC30A5.7b. c. elegans. [P13528-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M22363 Genomic DNA. Translation: AAA28158.1 .
M22363 Genomic DNA. Translation: AAA28159.1 .
FO080290 Genomic DNA. Translation: CCD62655.1 . Sequence problems.
FO080290 Genomic DNA. Translation: CCD62656.1 . Sequence problems.
PIRi A30042.
S44778.
RefSeqi NP_001021191.2. NM_001026020.4.
NP_498795.2. NM_066394.2.
UniGenei Cel.24958.

3D structure databases

ProteinModelPortali P13528.
SMRi P13528. Positions 269-420.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P13528. 1 interaction.
STRINGi 6239.C30A5.7b.

Proteomic databases

PaxDbi P13528.
PRIDEi P13528.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 176157.
KEGGi cel:CELE_C30A5.7.
UCSCi C30A5.7b. c. elegans. [P13528-1 ]

Organism-specific databases

CTDi 176157.
WormBasei C30A5.7a ; CE45098 ; WBGene00006818 ; unc-86.
C30A5.7b ; CE45067 ; WBGene00006818 ; unc-86.

Phylogenomic databases

eggNOGi NOG289030.
HOGENOMi HOG000116305.
InParanoidi P13528.
OrthoDBi EOG7RV9G7.
PhylomeDBi P13528.

Enzyme and pathway databases

SignaLinki P13528.

Miscellaneous databases

NextBioi 891370.

Family and domain databases

Gene3Di 1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProi IPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR015584. POU4-related.
IPR000327. POU_specific.
[Graphical view ]
PANTHERi PTHR11636:SF10. PTHR11636:SF10. 1 hit.
Pfami PF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view ]
PRINTSi PR00028. POUDOMAIN.
SMARTi SM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view ]
SUPFAMi SSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEi PS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The C. elegans cell lineage and differentiation gene unc-86 encodes a protein with a homeodomain and extended similarity to transcription factors."
    Finney M., Ruvkun G., Horvitz H.R.
    Cell 55:757-769(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS A AND B).
  2. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING.
    Strain: Bristol N2.
  3. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  4. "Mutations that lead to reiterations in the cell lineages of C. elegans."
    Chalfie M., Horvitz H.R., Sulston J.E.
    Cell 24:59-69(1981) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    Strain: Bristol N2.
  5. "The unc-86 gene product couples cell lineage and cell identity in C. elegans."
    Finney M., Ruvkun G.
    Cell 63:895-905(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  6. "Regulation of the mec-3 gene by the C.elegans homeoproteins UNC-86 and MEC-3."
    Xue D., Finney M., Ruvkun G., Chalfie M.
    EMBO J. 11:4969-4979(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Regulation of touch receptor differentiation by the Caenorhabditis elegans mec-3 and unc-86 genes."
    Duggan A., Ma C., Chalfie M.
    Development 125:4107-4119(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT.
    Strain: Bristol N2.
  8. "Protein interaction surface of the POU transcription factor UNC-86 selectively used in touch neurons."
    Rohrig S., Rockelein I., Donhauser R., Baumeister R.
    EMBO J. 19:3694-3703(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, DISRUPTION PHENOTYPE, MUTAGENESIS OF PRO-270 AND LEU-320.
  9. "The C. elegans POU-domain transcription factor UNC-86 regulates the tph-1 tryptophan hydroxylase gene and neurite outgrowth in specific serotonergic neurons."
    Sze J.Y., Zhang S., Li J., Ruvkun G.
    Development 129:3901-3911(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    Strain: Bristol N2.
  10. "Control of neuronal subtype identity by the C. elegans ARID protein CFI-1."
    Shaham S., Bargmann C.I.
    Genes Dev. 16:972-983(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
    Strain: Bristol N2.
  11. "Activity of the Caenorhabditis elegans UNC-86 POU transcription factor modulates olfactory sensitivity."
    Sze J.Y., Ruvkun G.
    Proc. Natl. Acad. Sci. U.S.A. 100:9560-9565(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
  12. "The POU transcription factor UNC-86 controls the timing and ventral guidance of Caenorhabditis elegans axon growth."
    Olsson-Carter K., Slack F.J.
    Dev. Dyn. 240:1815-1825(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    Strain: Bristol N2.
  13. "Dauer-specific dendrite arborization in C. elegans is regulated by KPC-1/Furin."
    Schroeder N.E., Androwski R.J., Rashid A., Lee H., Lee J., Barr M.M.
    Curr. Biol. 23:1527-1535(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Strain: Bristol N2.
  14. "The LIM and POU homeobox genes ttx-3 and unc-86 act as terminal selectors in distinct cholinergic and serotonergic neuron types."
    Zhang F., Bhattacharya A., Nelson J.C., Abe N., Gordon P., Lloret-Fernandez C., Maicas M., Flames N., Mann R.S., Colon-Ramos D.A., Hobert O.
    Development 141:422-435(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DNA-BINDING, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiUNC86_CAEEL
AccessioniPrimary (citable) accession number: P13528
Secondary accession number(s): D7SFI2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: September 3, 2014
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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