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Protein

Transcription factor unc-86

Gene

unc-86

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor required for correct cell fate determination and differentiation in diverse neuronal cell lineages where it plays a role in specifying the fate of daughter cells during cell divisions (PubMed:7237544, PubMed:2257628). Involved in sensory neuron production and function (PubMed:1361171, PubMed:9735371, PubMed:10899123). Binds both alone and with mec-3 to the mec-3 promoter to initiate and maintain mec-3 expression which is required for sensory neuron differentiation (PubMed:1361171, PubMed:9735371). In addition, binds both alone and with mec-3 to the promoters of mec-4 and mec-7 which act to regulate sensory neuron function (PubMed:9735371, PubMed:10899123). Involved in determining the identity of the serotonergic NSM neurons and the cholinergic IL2 sensory and URA motor neurons (PubMed:24353061). Promotes expression of the cfi-1 transcription factor in the URA and IL2 neurons which in turn activates normal URA and IL2 gene expression (PubMed:24353061, PubMed:11959845). Regulates expression of a number of genes in NSM neurons including bas-1, cat-1, dop-3, mgl-3, nlp-13, scd-2 and ptps-1 (PubMed:24353061). In the IL2 neurons, required for expression of cho-1, gcy-19, klp-6, lag-2, unc-5 and unc-17 (PubMed:24353061). Promotes expression of pkd-2 in the male-specific CEM head neurons (PubMed:11959845). Required for dauer-specific branching of IL2Q neurons and nictation behavior (PubMed:23932402). Controls both the timing and direction of axon outgrowth in HSN neurons (PubMed:21656875). Plays a role in serotonin production by regulating expression of the tryptophan hydrolase tph-1 which catalyzes serotonin synthesis, in the AIM, NSM, HSN and RIH neurons (PubMed:12135927). Involved in regulation of lin-11 expression in the AIZ interneurons, the major interneurons of the olfactory pathway, and is required for odortaxis behavior (PubMed:12883006). Involved in neurite pruning between AIM neurons during larval development by regulating the expression of transcription factor mbr-1 (PubMed:16139210). Required for correct localization of unc-40 (PubMed:24353061).12 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi363 – 422HomeoboxPROSITE-ProRule annotationAdd BLAST60

GO - Molecular functioni

  • RNA polymerase II regulatory region sequence-specific DNA binding Source: WormBase
  • RNA polymerase II transcription factor activity, sequence-specific DNA binding Source: WormBase
  • sequence-specific DNA binding Source: WormBase
  • transcription factor binding Source: WormBase

GO - Biological processi

  • mechanosensory behavior Source: WormBase
  • neuron development Source: WormBase
  • positive regulation of transcription from RNA polymerase II promoter Source: WormBase
  • positive regulation of transcription from RNA polymerase II promoter involved in neuron fate specification Source: WormBase
  • response to mechanical stimulus Source: WormBase
  • sensory perception of smell Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Neurogenesis, Olfaction, Sensory transduction, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

SignaLinkiP13528.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor unc-86
Alternative name(s):
Uncoordinated protein 86
Gene namesi
Name:unc-86
ORF Names:C30A5.7
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome III

Organism-specific databases

WormBaseiC30A5.7a; CE51471; WBGene00006818; unc-86.
C30A5.7b; CE45067; WBGene00006818; unc-86.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation1 Publication

GO - Cellular componenti

  • nucleus Source: WormBase
  • RNA polymerase II transcription factor complex Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

In a number of neuronal cell lineages, one of the two daughter cells of a division fails to assume its normal fate, retaining instead the fate of its parent cell. Altered gene expression in a number of neuron types including reduced cat-1 expression in AIM, NSM, HSN and RIH neurons, loss of cat-4 expression in HSN neurons, loss of expression of dop-3 and mgl-3 and reduced expression of bas-1, nlp-13, ptpa-1 and scd-2 in NSM neurons, loss of expression of gcy-19, klp-6, lag-2 and unc-17 and reduced expression of cho-1 and unc-5 in IL2 neurons, and loss of tph-1 expression in AIM, NSM, HSN and RIH neurons which leads to defects in serotonin production. Defective motility along with defects in IL2Q dendritic arborization and nictation. Abnormal neurite outgrowth in NSM neurons. Defective response to odor attractants but odor repellants are avoided normally. Penetrant ventral axon growth defects in HSN motor neurons with extension of processes in L1, three stages earlier than wild-type. Incorrect unc-40 localization. Defective egg-laying characterized by retention of substantially more eggs in the uterus of second-day animals than the wild-type.6 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi270P → S in u168; defective mechanosensation, lack of interaction with mec-3 and lack of maintained mec-3 expression. No egg-laying or Q neuroblast lineage defects. 1 Publication1
Mutagenesisi320L → F in u5; defective mechanosensation, lack of interaction with mec-3 and lack of maintained mec-3 expression. No egg-laying or Q neuroblast lineage defects. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001007801 – 467Transcription factor unc-86Add BLAST467

Proteomic databases

PaxDbiP13528.

Expressioni

Tissue specificityi

Specific to neurons and neuroblasts. Expressed in CEM head neurons and in IL2, URA, URB, URX and URY neurons. Not expressed in olfactory sensory neurons but expressed in AIZ interneurons.3 Publications

Developmental stagei

First appears during embryogenesis and persists throughout adulthood.1 Publication

Gene expression databases

BgeeiWBGene00006818.

Interactioni

Subunit structurei

Interacts with mec-3; the heterooligomer binds to the promoters of mec-3, mec-4 and mec-7.2 Publications

GO - Molecular functioni

  • transcription factor binding Source: WormBase

Protein-protein interaction databases

IntActiP13528. 1 interactor.
STRINGi6239.C30A5.7b.

Structurei

3D structure databases

ProteinModelPortaliP13528.
SMRiP13528.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini265 – 342POU-specificPROSITE-ProRule annotationAdd BLAST78

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi145 – 154POU-IV box10

Sequence similaritiesi

Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation
Contains 1 POU-specific domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiKOG1168. Eukaryota.
ENOG410XPNX. LUCA.
HOGENOMiHOG000116305.
InParanoidiP13528.
KOiK09366.
OrthoDBiEOG091G0WBK.
PhylomeDBiP13528.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_dom.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PRINTSiPR00028. POUDOMAIN.
SMARTiSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform b (identifier: P13528-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEKAHRFRLP FCSFFPVPLL VSVLIFHHSA PFLIQLFFPS PLFNPLLRPS
60 70 80 90 100
KISRGSENGA CTSHSTLQRT RKIIQWELPK RGGDQDIGDP RPFRIHLSPP
110 120 130 140 150
SFKVPLFSTD MQNTAPVPTT TTASKMQPFN NSLFGSFDDP ILNARAAQVA
160 170 180 190 200
LADIDVKNVP QLTNPLMRPH DMFSYSNYFS GIHDTSAATN IYQGLPSSSE
210 220 230 240 250
PFDASVVVPT SSDDQMTPLQ QVMAMQQSYG APPPFQYNMT HPFSTTSIAS
260 270 280 290 300
SNNLARYPIA PPTSDMDTDP RQLETFAEHF KQRRIKLGVT QADVGKALAH
310 320 330 340 350
LKMPGVGSLS QSTICRFESL TLSHNNMVAL KPILHSWLEK AEEAMKQKDT
360 370 380 390 400
IGDINGILPN TDKKRKRTSI AAPEKRELEQ FFKQQPRPSG ERIASIADRL
410 420 430 440 450
DLKKNVVRVW FCNQRQKQKR DFRSQFRARS AAAVMGPRVM PVLNGNNSNN
460
NLKQGQTTYN GLPGFFD
Length:467
Mass (Da):52,315
Last modified:January 1, 1990 - v1
Checksum:i86C3507584C91AC2
GO
Isoform a (identifier: P13528-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     129-134: FNNSLF → DFILFI
     135-172: Missing.

Show »
Length:429
Mass (Da):48,214
Checksum:i4389449360DC5FC9
GO

Sequence cautioni

The sequence CCD62655 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CCD62656 differs from that shown. Reason: Erroneous gene model prediction.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_002343129 – 134FNNSLF → DFILFI in isoform a. Curated6
Alternative sequenceiVSP_002344135 – 172Missing in isoform a. CuratedAdd BLAST38

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22363 Genomic DNA. Translation: AAA28158.1.
M22363 Genomic DNA. Translation: AAA28159.1.
FO080290 Genomic DNA. Translation: CCD62655.1. Sequence problems.
FO080290 Genomic DNA. Translation: CCD62656.1. Sequence problems.
PIRiA30042.
S44778.
RefSeqiNP_001021191.2. NM_001026020.4.
NP_001309473.1. NM_001322687.1.
UniGeneiCel.24958.

Genome annotation databases

GeneIDi176157.
KEGGicel:CELE_C30A5.7.
UCSCiC30A5.7b. c. elegans. [P13528-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22363 Genomic DNA. Translation: AAA28158.1.
M22363 Genomic DNA. Translation: AAA28159.1.
FO080290 Genomic DNA. Translation: CCD62655.1. Sequence problems.
FO080290 Genomic DNA. Translation: CCD62656.1. Sequence problems.
PIRiA30042.
S44778.
RefSeqiNP_001021191.2. NM_001026020.4.
NP_001309473.1. NM_001322687.1.
UniGeneiCel.24958.

3D structure databases

ProteinModelPortaliP13528.
SMRiP13528.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP13528. 1 interactor.
STRINGi6239.C30A5.7b.

Proteomic databases

PaxDbiP13528.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi176157.
KEGGicel:CELE_C30A5.7.
UCSCiC30A5.7b. c. elegans. [P13528-1]

Organism-specific databases

CTDi176157.
WormBaseiC30A5.7a; CE51471; WBGene00006818; unc-86.
C30A5.7b; CE45067; WBGene00006818; unc-86.

Phylogenomic databases

eggNOGiKOG1168. Eukaryota.
ENOG410XPNX. LUCA.
HOGENOMiHOG000116305.
InParanoidiP13528.
KOiK09366.
OrthoDBiEOG091G0WBK.
PhylomeDBiP13528.

Enzyme and pathway databases

SignaLinkiP13528.

Miscellaneous databases

PROiP13528.

Gene expression databases

BgeeiWBGene00006818.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_dom.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PRINTSiPR00028. POUDOMAIN.
SMARTiSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUNC86_CAEEL
AccessioniPrimary (citable) accession number: P13528
Secondary accession number(s): D7SFI2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: November 2, 2016
This is version 149 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.