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Protein

Transcription factor unc-86

Gene

unc-86

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription factor required for correct cell fate determination and differentiation in diverse neuronal cell lineages where it plays a role in specifying the fate of daughter cells during cell divisions (PubMed:7237544, PubMed:2257628). Involved in sensory neuron production and function (PubMed:1361171, PubMed:9735371, PubMed:10899123). Binds both alone and with mec-3 to the mec-3 promoter to initiate and maintain mec-3 expression which is required for sensory neuron differentiation (PubMed:1361171, PubMed:9735371). In addition, binds both alone and with mec-3 to the promoters of mec-4 and mec-7 which act to regulate sensory neuron function (PubMed:9735371, PubMed:10899123). Involved in determining the identity of the serotonergic NSM neurons and the cholinergic IL2 sensory and URA motor neurons (PubMed:24353061). Promotes expression of the cfi-1 transcription factor in the URA and IL2 neurons which in turn activates normal URA and IL2 gene expression (PubMed:24353061, PubMed:11959845). Regulates expression of a number of genes in NSM neurons including bas-1, cat-1, dop-3, mgl-3, nlp-13, scd-2 and ptps-1 (PubMed:24353061). In the IL2 neurons, required for expression of cho-1, gcy-19, klp-6, lag-2, unc-5 and unc-17 (PubMed:24353061). Promotes expression of pkd-2 in the male-specific CEM head neurons (PubMed:11959845). Required for dauer-specific branching of IL2Q neurons and nictation behavior (PubMed:23932402). Controls both the timing and direction of axon outgrowth in HSN neurons (PubMed:21656875). Plays a role in serotonin production by regulating expression of the tryptophan hydrolase tph-1 which catalyzes serotonin synthesis, in the AIM, NSM, HSN and RIH neurons (PubMed:12135927). Involved in regulation of lin-11 expression in the AIZ interneurons, the major interneurons of the olfactory pathway, and is required for odortaxis behavior (PubMed:12883006). Involved in neurite pruning between AIM neurons during larval development by regulating the expression of transcription factor mbr-1 (PubMed:16139210). Required for correct localization of unc-40 (PubMed:24353061).12 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi363 – 422HomeoboxPROSITE-ProRule annotationAdd BLAST60

GO - Molecular functioni

  • RNA polymerase II regulatory region sequence-specific DNA binding Source: WormBase
  • RNA polymerase II transcription factor activity, sequence-specific DNA binding Source: WormBase
  • sequence-specific DNA binding Source: WormBase
  • transcription factor binding Source: WormBase

GO - Biological processi

  • mechanosensory behavior Source: WormBase
  • neuron development Source: WormBase
  • positive regulation of transcription by RNA polymerase II Source: WormBase
  • positive regulation of transcription from RNA polymerase II promoter involved in neuron fate specification Source: WormBase
  • response to mechanical stimulus Source: WormBase
  • sensory perception of smell Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionDNA-binding
Biological processNeurogenesis, Olfaction, Sensory transduction, Transcription, Transcription regulation

Enzyme and pathway databases

SignaLinkiP13528

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor unc-86
Alternative name(s):
Uncoordinated protein 86
Gene namesi
Name:unc-86
ORF Names:C30A5.7
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome III

Organism-specific databases

WormBaseiC30A5.7a ; CE51471 ; WBGene00006818 ; unc-86
C30A5.7b ; CE45067 ; WBGene00006818 ; unc-86

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

In a number of neuronal cell lineages, one of the two daughter cells of a division fails to assume its normal fate, retaining instead the fate of its parent cell. Altered gene expression in a number of neuron types including reduced cat-1 expression in AIM, NSM, HSN and RIH neurons, loss of cat-4 expression in HSN neurons, loss of expression of dop-3 and mgl-3 and reduced expression of bas-1, nlp-13, ptpa-1 and scd-2 in NSM neurons, loss of expression of gcy-19, klp-6, lag-2 and unc-17 and reduced expression of cho-1 and unc-5 in IL2 neurons, and loss of tph-1 expression in AIM, NSM, HSN and RIH neurons which leads to defects in serotonin production. Defective motility along with defects in IL2Q dendritic arborization and nictation. Abnormal neurite outgrowth in NSM neurons. Defective response to odor attractants but odor repellants are avoided normally. Penetrant ventral axon growth defects in HSN motor neurons with extension of processes in L1, three stages earlier than wild-type. Incorrect unc-40 localization. Defective egg-laying characterized by retention of substantially more eggs in the uterus of second-day animals than the wild-type.6 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi270P → S in u168; defective mechanosensation, lack of interaction with mec-3 and lack of maintained mec-3 expression. No egg-laying or Q neuroblast lineage defects. 1 Publication1
Mutagenesisi320L → F in u5; defective mechanosensation, lack of interaction with mec-3 and lack of maintained mec-3 expression. No egg-laying or Q neuroblast lineage defects. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001007801 – 467Transcription factor unc-86Add BLAST467

Proteomic databases

EPDiP13528
PaxDbiP13528
PRIDEiP13528

Expressioni

Tissue specificityi

Specific to neurons and neuroblasts. Expressed in CEM head neurons and in IL2, URA, URB, URX and URY neurons. Not expressed in olfactory sensory neurons but expressed in AIZ interneurons.3 Publications

Developmental stagei

First appears during embryogenesis and persists throughout adulthood.1 Publication

Gene expression databases

BgeeiWBGene00006818

Interactioni

Subunit structurei

Interacts with mec-3; the heterooligomer binds to the promoters of mec-3, mec-4 and mec-7.2 Publications

GO - Molecular functioni

  • transcription factor binding Source: WormBase

Protein-protein interaction databases

IntActiP13528, 1 interactor
STRINGi6239.C30A5.7b

Structurei

3D structure databases

ProteinModelPortaliP13528
SMRiP13528
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini265 – 342POU-specificPROSITE-ProRule annotationAdd BLAST78

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi145 – 154POU-IV box10

Sequence similaritiesi

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiKOG1168 Eukaryota
ENOG410XPNX LUCA
HOGENOMiHOG000116305
InParanoidiP13528
KOiK09366
OrthoDBiEOG091G0WBK
PhylomeDBiP13528

Family and domain databases

CDDicd00086 homeodomain, 1 hit
InterProiView protein in InterPro
IPR009057 Homeobox-like_sf
IPR017970 Homeobox_CS
IPR001356 Homeobox_dom
IPR010982 Lambda_DNA-bd_dom_sf
IPR013847 POU
IPR000327 POU_dom
PfamiView protein in Pfam
PF00046 Homeobox, 1 hit
PF00157 Pou, 1 hit
PRINTSiPR00028 POUDOMAIN
SMARTiView protein in SMART
SM00389 HOX, 1 hit
SM00352 POU, 1 hit
SUPFAMiSSF46689 SSF46689, 1 hit
SSF47413 SSF47413, 1 hit
PROSITEiView protein in PROSITE
PS00027 HOMEOBOX_1, 1 hit
PS50071 HOMEOBOX_2, 1 hit
PS00035 POU_1, 1 hit
PS00465 POU_2, 1 hit
PS51179 POU_3, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform b (identifier: P13528-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEKAHRFRLP FCSFFPVPLL VSVLIFHHSA PFLIQLFFPS PLFNPLLRPS
60 70 80 90 100
KISRGSENGA CTSHSTLQRT RKIIQWELPK RGGDQDIGDP RPFRIHLSPP
110 120 130 140 150
SFKVPLFSTD MQNTAPVPTT TTASKMQPFN NSLFGSFDDP ILNARAAQVA
160 170 180 190 200
LADIDVKNVP QLTNPLMRPH DMFSYSNYFS GIHDTSAATN IYQGLPSSSE
210 220 230 240 250
PFDASVVVPT SSDDQMTPLQ QVMAMQQSYG APPPFQYNMT HPFSTTSIAS
260 270 280 290 300
SNNLARYPIA PPTSDMDTDP RQLETFAEHF KQRRIKLGVT QADVGKALAH
310 320 330 340 350
LKMPGVGSLS QSTICRFESL TLSHNNMVAL KPILHSWLEK AEEAMKQKDT
360 370 380 390 400
IGDINGILPN TDKKRKRTSI AAPEKRELEQ FFKQQPRPSG ERIASIADRL
410 420 430 440 450
DLKKNVVRVW FCNQRQKQKR DFRSQFRARS AAAVMGPRVM PVLNGNNSNN
460
NLKQGQTTYN GLPGFFD
Length:467
Mass (Da):52,315
Last modified:January 1, 1990 - v1
Checksum:i86C3507584C91AC2
GO
Isoform a (identifier: P13528-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     129-134: FNNSLF → DFILFI
     135-172: Missing.

Show »
Length:429
Mass (Da):48,214
Checksum:i4389449360DC5FC9
GO

Sequence cautioni

The sequence CCD62655 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CCD62656 differs from that shown. Reason: Erroneous gene model prediction.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_002343129 – 134FNNSLF → DFILFI in isoform a. Curated6
Alternative sequenceiVSP_002344135 – 172Missing in isoform a. CuratedAdd BLAST38

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22363 Genomic DNA Translation: AAA28158.1
M22363 Genomic DNA Translation: AAA28159.1
FO080290 Genomic DNA Translation: CCD62655.1 Sequence problems.
FO080290 Genomic DNA Translation: CCD62656.1 Sequence problems.
PIRiA30042
S44778
RefSeqiNP_001021191.2, NM_001026020.4
NP_001309473.1, NM_001322687.1
UniGeneiCel.24958

Genome annotation databases

GeneIDi176157
KEGGicel:CELE_C30A5.7
UCSCiC30A5.7b c. elegans [P13528-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiUNC86_CAEEL
AccessioniPrimary (citable) accession number: P13528
Secondary accession number(s): D7SFI2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: May 23, 2018
This is version 157 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

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