P13522 (SCRB_STRMU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sucrose-6-phosphate hydrolase
Short name=Sucrase
EC=3.2.1.26

Alternative name(s):
Invertase

Gene names

Name:	scrB
Ordered Locus Names:	SMU_1843

Organism

Streptococcus mutans [Complete proteome] [HAMAP]

Taxonomic identifier

1309 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	479 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides.
Pathway	Glycan biosynthesis; sucrose metabolism.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the glycosyl hydrolase 32 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Cellular component	Cytoplasm
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	sucrose alpha-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 479	479	Sucrose-6-phosphate hydrolase		PRO_0000169876
Regions
	Region	44 – 47	4	Substrate binding By similarity
	Region	106 – 107	2	Substrate binding By similarity
	Region	166 – 167	2	Substrate binding By similarity
Sites
	Active site	47	1	By similarity
	Binding site	63	1	Substrate By similarity
	Binding site	223	1	Substrate By similarity
Experimental info
	Sequence conflict	188 – 212	25	Missing Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P13522 [UniParc]. Last modified November 28, 2002. Version 3. Checksum: D44A6810CB68CA1C	FASTA	479	54,672
	10 20 30 40 50 60 MNLPQNIRYR RYQDWTEEEI KSIKTNVALS PWHTTYHIEP KTGLLNDPNG FSYFNGKFNL 70 80 90 100 110 120 FYQNWPFGAA HGLKSWIHTE SEDLVHFKET GTVLYPDTSH DSHGAYSGSA YEIGDQLFLF 130 140 150 160 170 180 YTGNVRDENW VRHPLQIGAF MDKKGNIQKF TDVLIKQPND VTEHFRDPQI FNYKGQFYAI 190 200 210 220 230 240 VGAQSLDKKG FIKLYKAVDN DIKNWQEVGN LDFGGSKSEY MIECPNLVFI NEQPVLIYSP 250 260 270 280 290 300 QGLSKSELDY HNIYPNTYKV CQSFDTEKPA LVDASEIQNL DFGFECYATQ AFNAPDGRVY 310 320 330 340 350 360 AVSWIGLPDI DYPSDSYDYQ GALSLVKELS LKHGKLYQYP VEAVRSLRSE KEAVTYKPET 370 380 390 400 410 420 NNTYELELTF DSSSVNELLL FADNKGNGLA ITVDTKMGTI LIDRSKAGEQ YALEFGSQRS 430 440 450 460 470 CSIQAKETVV NIFVDKSIFE IFINKGEKVF TGRVFPNDKQ TGIVIKSGKP SGNYYELKY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of the Streptococcus mutans scrB gene." Sato Y., Kuramitsu H.K. Infect. Immun. 56:1956-1960(1988) [PubMed: 3397182] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: GS-5.
[2]	"Genome sequence of Streptococcus mutans UA159, a cariogenic dental pathogen." Ajdic D.J., McShan W.M., McLaughlin R.E., Savic G., Chang J., Carson M.B., Primeaux C., Tian R., Kenton S., Jia H.G., Lin S.P., Qian Y., Li S., Zhu H., Najar F.Z., Lai H., White J., Roe B.A., Ferretti J.J. Proc. Natl. Acad. Sci. U.S.A. 99:14434-14439(2002) [PubMed: 12397186] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700610 / UA159 / Serotype c.
[3]	"Characterization and sequence analysis of the scrA gene encoding enzyme IIScr of the Streptococcus mutans phosphoenolpyruvate-dependent sucrose phosphotransferase system." Sato Y., Poy F., Jacobson G.R., Kuramitsu H.K. J. Bacteriol. 171:263-271(1989) [PubMed: 2536656] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-9. Strain: GS-5.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M36849 Genomic DNA. Translation: AAA26972.1. X51507 Genomic DNA. Translation: CAA35872.1. AE014133 Genomic DNA. Translation: AAN59465.1. M22711 Genomic DNA. Translation: AAA26970.2.
PIR	A43501.
RefSeq	NP_722159.1. NC_004350.2.
3D structure databases
ProteinModelPortal	P13522.
ModBase	Search...
Protein family/group databases
CAZy	GH32. Glycoside Hydrolase Family 32.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTRT00000013457; EBSTRP00000012939; EBSTRG00000013457.
GeneID	1029055.
GenomeReviews	Gene locus SMU_1843 in contig AE014133_GR.
KEGG	smu:SMU_1843.
NMPDR	fig|210007.1.peg.1672.
PATRIC	19665681. VBIStrMut61772_1645.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000026668.
HOGENOM	HBG693902.
OMA	FNYKGQF.
ProtClustDB	CLSK2756471.
Enzyme and pathway databases
BioCyc	SMUT210007:SMU_1843-MONOMER.
Family and domain databases
InterPro	IPR008985. ConA-like_lec_gl. IPR001362. Glyco_hydro_32. IPR018053. Glyco_hydro_32_AS. IPR013189. Glyco_hydro_32_C. IPR013148. Glyco_hydro_32_N. IPR023296. Glyco_hydro_43_beta-prop. IPR006232. Suc6P_hydrolase. [Graphical view]
Gene3D	G3DSA:2.115.10.20. Glyco_hydro_43_beta-prop. 1 hit.
KO	K01193.
Pfam	PF08244. Glyco_hydro_32C. 1 hit. PF00251. Glyco_hydro_32N. 1 hit. [Graphical view]
SMART	SM00640. Glyco_32. 1 hit. [Graphical view]
SUPFAM	SSF49899. ConA_like_lec_gl. 1 hit. SSF75005. Glyco_hydro_43_beta-prop. 1 hit.
TIGRFAMs	TIGR01322. ScrB_fam. 1 hit.
PROSITE	PS00609. GLYCOSYL_HYDROL_F32. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SCRB_STRMU

Accession

Primary (citable) accession number: P13522

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	November 28, 2002
Last modified:	January 25, 2012
This is version 93 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program