F-actin-capping protein subunit beta - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P13517 (CAPZB_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 141. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
F-actin-capping protein subunit beta

Gene names

Name:	CAP2
Ordered Locus Names:	YIL034C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	287 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	F-actin-capping proteins bind in a Ca²⁺-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments. Ref.6
Subunit structure	Heterodimer of an alpha and a beta subunit. Ref.6
Subcellular location	Cytoplasm › cytoskeleton › actin patch. Bud. Bud tip. Note: Found at cortical actin spots at the site of bud emergence and at the tips of growing buds and shmoos. Ref.6
Miscellaneous	Present with 6770 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the F-actin-capping protein beta subunit family.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton
Ligand	Actin-binding
Molecular function	Actin capping
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	actin cytoskeleton organization Inferred from electronic annotation. Source: InterPro barbed-end actin filament capping Inferred from direct assay PubMed 14769858. Source: SGD filamentous growth Inferred from mutant phenotype PubMed 15645503. Source: SGD
Cellular_component	F-actin capping protein complex Inferred from physical interaction PubMed 14769858. Source: SGD WASH complex Inferred from electronic annotation. Source: InterPro actin cortical patch Inferred from direct assay PubMed 14769858. Source: SGD actin filament Inferred from physical interaction PubMed 14769858. Source: SGD cellular bud tip Inferred from direct assay PubMed 22842922. Source: SGD mating projection tip Inferred from direct assay PubMed 19053807. Source: SGD plasma membrane Inferred from direct assay PubMed 22842922. Source: SGD
Molecular_function	actin filament binding Inferred from sequence or structural similarity PubMed 14769858. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CAP1	P28495	5	EBI-4013,EBI-4003

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 287

287

F-actin-capping protein subunit beta

PRO_0000204642

Amino acid modifications

Modified residue

Phosphoserine Ref.8 Ref.9

Modified residue

Phosphoserine Ref.9

Modified residue

267

Phosphoserine Ref.9

Modified residue

268

Phosphoserine Ref.9

Experimental info

Sequence conflict

140

F → Y in AAA66935. Ref.5

Sequence conflict

146 – 147

FK → I in AAA66935. Ref.5

Sequences

Sequence

Length

Mass (Da)

Tools

P13517 [UniParc].

Last modified November 1, 1991. Version 3.
Checksum: 6E669023D1378778
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FASTA

287

32,629

        10         20         30         40         50         60 
MSDAQFDAAL DLLRRLNPTT LQENLNNLIE LQPNLAQDLL SSVDVPLSTQ KDSADSNREY 

        70         80         90        100        110        120 
LCCDYNRDID SFRSPWSNTY YPELSPKDLQ DSPFPSAPLR KLEILANDSF DVYRDLYYEG 

       130        140        150        160        170        180 
GISSVYLWDL NEEDFNGHDF AGVVLFKKNQ SDHSNWDSIH VFEVTTSPSS PDSFNYRVTT 

       190        200        210        220        230        240 
TIILHLDKTK TDQNSHMMLS GNLTRQTEKD IAIDMSRPLD VIFTSHVANL GSLIEDIESQ 

       250        260        270        280 
MRNLLETVYF EKTRDIFHQT KNAAIASSAE EANKDAQAEV IRGLQSL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Disruption of the actin cytoskeleton in yeast capping protein mutants." Amatruda J.F., Cannon J.F., Tatchell K., Hug C., Cooper J.A. Nature 344:352-354(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Amatruda J.F., Gattermeir D.J., Cooper J.A. Submitted (OCT-1991) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SEQUENCE REVISION.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX." Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. Barrell B.G. Nature 387:84-87(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Characterization of Saccharomyces cerevisiae genes encoding subunits of cyclic AMP-dependent protein kinase." Cannon J.F., Tatchell K. Mol. Cell. Biol. 7:2653-2663(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-195.
[6]	"Purification, characterization, and immunofluorescence localization of Saccharomyces cerevisiae capping protein." Amatruda J.F., Cooper J.A. J. Cell Biol. 117:1067-1076(1992) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 149-156, FUNCTION, SUBUNIT, SUBCELLULAR LOCATION.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-85, MASS SPECTROMETRY. Strain: ADR376.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-85; SER-92; SER-267 AND SER-268, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X62630 Genomic DNA. Translation: CAA44497.1. Z46861 Genomic DNA. Translation: CAA86917.1. M17223 Genomic DNA. Translation: AAA66935.1. BK006942 Genomic DNA. Translation: DAA08514.1.
PIR	S49944.
RefSeq	NP_012230.3. NM_001179384.3.
3D structure databases
ProteinModelPortal	P13517.
SMR	P13517. Positions 2-256.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-836N.
IntAct	P13517. 12 interactions.
MINT	MINT-482123.
STRING	4932.YIL034C.
Proteomic databases
PaxDb	P13517.
PeptideAtlas	P13517.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YIL034C; YIL034C; YIL034C.
GeneID	854777.
KEGG	sce:YIL024C. sce:YIL034C.
Organism-specific databases
CYGD	YIL034c.
SGD	S000001296. CAP2.
Phylogenomic databases
eggNOG	NOG291067.
GeneTree	ENSGT00390000017957.
HOGENOM	HOG000041208.
KO	K10365.
OMA	NLLQEVY.
OrthoDB	EOG4XWK6P.
Gene expression databases
Genevestigator	P13517.
GermOnline	YIL034C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR019771. F-actin_capping_bsu_CS. IPR001698. WASH_F-actin_cap_beta. [Graphical view]
PANTHER	PTHR10619. PTHR10619. 1 hit.
Pfam	PF01115. F_actin_cap_B. 1 hit. [Graphical view]
PRINTS	PR00192. FACTINCAPB.
PROSITE	PS00231. F_ACTIN_CAPPING_BETA. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	977553.

Entry information

Entry name

CAPZB_YEAST

Accession

Primary (citable) accession number: P13517
Secondary accession number(s): D6VVP8, Q07082

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	November 1, 1991
Last modified:	May 1, 2013
This is version 141 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome IX

Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents