Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P13517 (CAPZB_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 151. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
F-actin-capping protein subunit beta
Gene names
Name:CAP2
Ordered Locus Names:YIL034C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length287 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

F-actin-capping proteins bind in a Ca2+-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments. Ref.6

Subunit structure

Heterodimer of an alpha and a beta subunit. Ref.6

Subcellular location

Cytoplasmcytoskeletonactin patch. Bud. Bud tip. Note: Found at cortical actin spots at the site of bud emergence and at the tips of growing buds and shmoos. Ref.6

Miscellaneous

Present with 6770 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the F-actin-capping protein beta subunit family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

CAP1P284955EBI-4013,EBI-4003

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.11
Chain2 – 287286F-actin-capping protein subunit beta
PRO_0000204642

Amino acid modifications

Modified residue21N-acetylserine Ref.11
Modified residue851Phosphoserine Ref.8 Ref.9
Modified residue921Phosphoserine Ref.10

Experimental info

Sequence conflict1401F → Y in AAA66935. Ref.5
Sequence conflict146 – 1472FK → I in AAA66935. Ref.5

Sequences

Sequence LengthMass (Da)Tools
P13517 [UniParc].

Last modified November 1, 1991. Version 3.
Checksum: 6E669023D1378778

FASTA28732,629
        10         20         30         40         50         60 
MSDAQFDAAL DLLRRLNPTT LQENLNNLIE LQPNLAQDLL SSVDVPLSTQ KDSADSNREY 

        70         80         90        100        110        120 
LCCDYNRDID SFRSPWSNTY YPELSPKDLQ DSPFPSAPLR KLEILANDSF DVYRDLYYEG 

       130        140        150        160        170        180 
GISSVYLWDL NEEDFNGHDF AGVVLFKKNQ SDHSNWDSIH VFEVTTSPSS PDSFNYRVTT 

       190        200        210        220        230        240 
TIILHLDKTK TDQNSHMMLS GNLTRQTEKD IAIDMSRPLD VIFTSHVANL GSLIEDIESQ 

       250        260        270        280 
MRNLLETVYF EKTRDIFHQT KNAAIASSAE EANKDAQAEV IRGLQSL 

« Hide

References

« Hide 'large scale' references
[1]"Disruption of the actin cytoskeleton in yeast capping protein mutants."
Amatruda J.F., Cannon J.F., Tatchell K., Hug C., Cooper J.A.
Nature 344:352-354(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]Amatruda J.F., Gattermeir D.J., Cooper J.A.
Submitted (OCT-1991) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SEQUENCE REVISION.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX."
Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. expand/collapse author list , Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:84-87(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Characterization of Saccharomyces cerevisiae genes encoding subunits of cyclic AMP-dependent protein kinase."
Cannon J.F., Tatchell K.
Mol. Cell. Biol. 7:2653-2663(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-195.
[6]"Purification, characterization, and immunofluorescence localization of Saccharomyces cerevisiae capping protein."
Amatruda J.F., Cooper J.A.
J. Cell Biol. 117:1067-1076(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 149-156, FUNCTION, SUBUNIT, SUBCELLULAR LOCATION.
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-85, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[9]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-85, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-92, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X62630 Genomic DNA. Translation: CAA44497.1.
Z46861 Genomic DNA. Translation: CAA86917.1.
M17223 Genomic DNA. Translation: AAA66935.1.
BK006942 Genomic DNA. Translation: DAA08514.1.
PIRS49944.
RefSeqNP_012230.3. NM_001179384.3.

3D structure databases

ProteinModelPortalP13517.
SMRP13517. Positions 2-256.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34956. 78 interactions.
DIPDIP-836N.
IntActP13517. 11 interactions.
MINTMINT-482123.
STRING4932.YIL034C.

Proteomic databases

MaxQBP13517.
PaxDbP13517.
PeptideAtlasP13517.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYIL034C; YIL034C; YIL034C.
GeneID854777.
KEGGsce:YIL034C.

Organism-specific databases

CYGDYIL034c.
SGDS000001296. CAP2.

Phylogenomic databases

eggNOGNOG291067.
GeneTreeENSGT00390000017957.
HOGENOMHOG000041208.
KOK10365.
OMAFDTYREM.
OrthoDBEOG78H45S.

Enzyme and pathway databases

BioCycYEAST:G3O-31306-MONOMER.

Gene expression databases

GenevestigatorP13517.

Family and domain databases

InterProIPR001698. CapZ_beta.
IPR019771. F-actin_capping_bsu_CS.
[Graphical view]
PANTHERPTHR10619. PTHR10619. 1 hit.
PfamPF01115. F_actin_cap_B. 1 hit.
[Graphical view]
PRINTSPR00192. FACTINCAPB.
PROSITEPS00231. F_ACTIN_CAPPING_BETA. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio977553.
PROP13517.

Entry information

Entry nameCAPZB_YEAST
AccessionPrimary (citable) accession number: P13517
Secondary accession number(s): D6VVP8, Q07082
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: November 1, 1991
Last modified: June 11, 2014
This is version 151 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome IX

Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families