Reviewed, UniProtKB/Swiss-Prot P13516 (ACOD1_MOUSE)

Names and origin

Protein names

Recommended name:
    Acyl-CoA desaturase 1
    EC=1.14.19.1
Alternative name(s):
    Stearoyl-CoA desaturase 1
    Fatty acid desaturase 1
    Delta(9)-desaturase 1

Gene names

Name:

Scd1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	355 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Terminal component of the liver microsomal stearyl-CoA desaturase system, that utilizes O2 and electrons from reduced cytochrome b5 to catalyze the insertion of a double bond into a spectrum of fatty acyl-CoA substrates including palmitoyl-CoA and stearoyl-CoA.
Catalytic activity	Stearoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ = oleoyl-CoA + 2 ferricytochrome b5 + 2 H2O.
Cofactor	Iron.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein Probable.
Domain	The histidine box domains may contain the active site and/or be involved in metal ion binding.
Sequence similarities	Belongs to the fatty acid desaturase family.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Ligand	Iron
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	brown fat cell differentiation Inferred from direct assay. Source: MGI fatty acid biosynthetic process Inferred from mutant phenotype. Source: MGI oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW white fat cell differentiation Inferred from direct assay. Source: MGI
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW stearoyl-CoA 9-desaturase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 355	355	Acyl-CoA desaturase 1		PRO_0000185397
Regions
	Transmembrane	72 – 92	21	Potential
	Transmembrane	94 – 114	21	Potential
	Transmembrane	219 – 239	21	Potential
	Transmembrane	311 – 331	21	Potential
	Motif	116 – 121	6	Histidine box-1
	Motif	153 – 157	5	Histidine box-2
	Motif	294 – 298	5	Histidine box-3
Experimental info
	Sequence conflict	97	1	C → A in AAA40103. Ref.1
	Sequence conflict	148	1	E → D in AAA40103. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P13516-1 [UniParc]. Last modified June 6, 2002. Version 2. Checksum: 00E2348C1898FE75	FASTA	355	41,046
	10 20 30 40 50 60 MPAHMLQEIS SSYTTTTTIT APPSGNEREK VKTVPLHLEE DIRPEMKEDI HDPTYQDEEG 70 80 90 100 110 120 PPPKLEYVWR NIILMVLLHL GGLYGIILVP SCKLYTCLFG IFYYMTSALG ITAGAHRLWS 130 140 150 160 170 180 HRTYKARLPL RIFLIIANTM AFQNDVYEWA RDHRAHHKFS ETHADPHNSR RGFFFSHVGW 190 200 210 220 230 240 LLVRKHPAVK EKGGKLDMSD LKAEKLVMFQ RRYYKPGLLL MCFILPTLVP WYCWGETFVN 250 260 270 280 290 300 SLFVSTFLRY TLVLNATWLV NSAAHLYGYR PYDKNIQSRE NILVSLGAVG EGFHNYHHTF 310 320 330 340 350 PFDYSASEYR WHINFTTFFI DCMAALGLAY DRKKVSKATV LARIKRTGDG SHKSS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Differentiation-induced gene expression in 3T3-L1 preadipocytes. Characterization of a differentially expressed gene encoding stearoyl-CoA desaturase." Ntambi J.M., Buhrow S.A., Kaestner K.H., Christy R.J., Sibley E., Kelly T.J. Jr., Lane M.D. J. Biol. Chem. 263:17291-17300(1988) [PubMed: 2903162] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Adipocyte.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Mammary gland.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M21285 , M21280, M21281, M21282, M21283, M21284 Genomic DNA. Translation: AAA40103.1. BC007474 mRNA. Translation: AAH07474.1. BC055453 mRNA. Translation: AAH55453.1.
IPI	IPI00322530.
PIR	A32115.
RefSeq	NP_033153.2.
UniGene	Mm.267377
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	P13516.
Genome annotation databases
Ensembl	ENSMUSG00000037071. Mus musculus. [Contig view]
GeneID	20249.
KEGG	mmu:20249.
Organism-specific databases
MGI	MGI:98239. Scd1.
Phylogenomic databases
HOGENOM	P13516.
HOVERGEN	P13516.
OMA	P13516. EEMSSSY.
Enzyme and pathway databases
BRENDA	1.14.19.1. 244.
Gene expression databases
ArrayExpress	P13516.
Bgee	P13516.
CleanEx	MM_SCD1.
GermOnline	ENSMUSG00000037071. Mus musculus.
Family and domain databases
InterPro	IPR005804. Fatty_acid_desaturase-1. IPR001522. Fatty_acid_desaturase-1_C. IPR015876. Fatty_acid_desaturase-1_core. [Graphical view]
Pfam	PF00487. FA_desaturase. 1 hit. [Graphical view]
PRINTS	PR00075. FACDDSATRASE.
ProDom	PD002221. Desaturase. 1 hit. PD001081. FA_desat_sub. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00476. FATTY_ACID_DESATUR_1. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	297897.
SOURCE	Search...

Entry information

Entry name

ACOD1_MOUSE

Accession

Primary (citable) accession number: P13516
Secondary accession number(s): Q922I6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	June 6, 2002
Last modified:	June 16, 2009
This is version 92 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)