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Protein

Lipoprotein signal peptidase

Gene

lspA

Organism
Enterobacter aerogenes (strain ATCC 13048 / DSM 30053 / JCM 1235 / KCTC 2190 / NBRC 13534 / NCIMB 10102 / NCTC 10006) (Aerobacter aerogenes)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

This protein specifically catalyzes the removal of signal peptides from prolipoproteins.

Catalytic activityi

Release of signal peptides from bacterial membrane prolipoproteins. Hydrolyzes -Xaa-Yaa-Zaa-|-(S,diacylglyceryl)Cys-, in which Xaa is hydrophobic (preferably Leu), and Yaa (Ala or Ser) and Zaa (Gly or Ala) have small, neutral side chains.

Pathwayi: lipoprotein biosynthesis (signal peptide cleavage)

This protein is involved in the pathway lipoprotein biosynthesis (signal peptide cleavage), which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway lipoprotein biosynthesis (signal peptide cleavage) and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei114 – 1141By similarity
Active sitei141 – 1411By similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Aspartyl protease, Hydrolase, Protease

Enzyme and pathway databases

BioCyciEAER1028307:GHNA-2216-MONOMER.
UniPathwayiUPA00665.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoprotein signal peptidase (EC:3.4.23.36)
Alternative name(s):
Prolipoprotein signal peptidase
Signal peptidase II
Short name:
SPase II
Gene namesi
Name:lspA
Synonyms:lsp
Ordered Locus Names:EAE_10850
OrganismiEnterobacter aerogenes (strain ATCC 13048 / DSM 30053 / JCM 1235 / KCTC 2190 / NBRC 13534 / NCIMB 10102 / NCTC 10006) (Aerobacter aerogenes)
Taxonomic identifieri1028307 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella
Proteomesi
  • UP000008881 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1111CytoplasmicSequence analysisAdd
BLAST
Transmembranei12 – 2211HelicalSequence analysisAdd
BLAST
Topological domaini23 – 6947PeriplasmicSequence analysisAdd
BLAST
Transmembranei70 – 8819HelicalSequence analysisAdd
BLAST
Topological domaini89 – 957CytoplasmicSequence analysis
Transmembranei96 – 11318HelicalSequence analysisAdd
BLAST
Topological domaini114 – 13825PeriplasmicSequence analysisAdd
BLAST
Transmembranei139 – 15315HelicalSequence analysisAdd
BLAST
Topological domaini154 – 16512CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 165165Lipoprotein signal peptidasePRO_0000178781Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi1028307.EAE_10850.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase A8 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105M02. Bacteria.
COG0597. LUCA.
KOiK03101.
OMAiIWLWYRT.
OrthoDBiPOG091H059F.

Family and domain databases

HAMAPiMF_00161. LspA. 1 hit.
InterProiIPR001872. Peptidase_A8.
[Graphical view]
PANTHERiPTHR33695:SF1. PTHR33695:SF1. 1 hit.
PfamiPF01252. Peptidase_A8. 1 hit.
[Graphical view]
PRINTSiPR00781. LIPOSIGPTASE.
TIGRFAMsiTIGR00077. lspA. 1 hit.
PROSITEiPS00855. SPASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P13514-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKSICSTGL RWLWVVVAVL IIDLGSKFLI LQNFALGETV SLFPSLNLHY
60 70 80 90 100
ARNYGAAFSF LADSGGWQRW FFAGIAVGIC VVLAVLMYRS KATQKLNNIA
110 120 130 140 150
YALIIGGALG NLFDRLWHGF VVDMIDFYVG DWHFATFNLA DSAICIGAAL
160
IVLEGFLPSS DKKTS
Length:165
Mass (Da):18,107
Last modified:January 1, 1990 - v1
Checksum:iA47A352CB1616724
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26713 Genomic DNA. Translation: AAA24804.1.
CP002824 Genomic DNA. Translation: AEG97086.1.
RefSeqiWP_015368327.1. NC_015663.1.
YP_004592365.1. NC_015663.1.

Genome annotation databases

EnsemblBacteriaiAEG97086; AEG97086; EAE_10850.
GeneIDi10792374.
KEGGieae:EAE_10850.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26713 Genomic DNA. Translation: AAA24804.1.
CP002824 Genomic DNA. Translation: AEG97086.1.
RefSeqiWP_015368327.1. NC_015663.1.
YP_004592365.1. NC_015663.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi1028307.EAE_10850.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAEG97086; AEG97086; EAE_10850.
GeneIDi10792374.
KEGGieae:EAE_10850.

Phylogenomic databases

eggNOGiENOG4105M02. Bacteria.
COG0597. LUCA.
KOiK03101.
OMAiIWLWYRT.
OrthoDBiPOG091H059F.

Enzyme and pathway databases

UniPathwayiUPA00665.
BioCyciEAER1028307:GHNA-2216-MONOMER.

Family and domain databases

HAMAPiMF_00161. LspA. 1 hit.
InterProiIPR001872. Peptidase_A8.
[Graphical view]
PANTHERiPTHR33695:SF1. PTHR33695:SF1. 1 hit.
PfamiPF01252. Peptidase_A8. 1 hit.
[Graphical view]
PRINTSiPR00781. LIPOSIGPTASE.
TIGRFAMsiTIGR00077. lspA. 1 hit.
PROSITEiPS00855. SPASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLSPA_ENTAK
AccessioniPrimary (citable) accession number: P13514
Secondary accession number(s): G0E467
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: September 7, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.