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P13513

- TRI5_FUSSP

UniProt

P13513 - TRI5_FUSSP

Protein

Trichodiene synthase

Gene

TRI5

Organism
Fusarium sporotrichioides
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 1 (01 Jan 1990)
      Previous versions | rss
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    Functioni

    TS is a member of the terpene cyclase group of enzymes. It catalyzes the isomerization and cyclization of farnesyl pyro-phosphate to form trichodiene, the first cyclic intermediate in the biosynthetic pathway for trichothecenes. It serves to branch trichothecene biosynthesis from the isoprenoid pathway.

    Catalytic activityi

    (2E,6E)-farnesyl diphosphate = trichodiene + diphosphate.

    Pathwayi

    GO - Molecular functioni

    1. trichodiene synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. sesquiterpenoid biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Lyase

    Enzyme and pathway databases

    BRENDAi4.2.3.6. 2364.
    UniPathwayiUPA00267.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Trichodiene synthase (EC:4.2.3.6)
    Alternative name(s):
    Sesquiterpene cyclase
    Short name:
    TS
    Gene namesi
    Name:TRI5
    Synonyms:TOX 5
    OrganismiFusarium sporotrichioides
    Taxonomic identifieri5514 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesHypocreomycetidaeHypocrealesNectriaceaeFusarium

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 374374Trichodiene synthasePRO_0000221584Add
    BLAST

    Structurei

    Secondary structure

    1
    374
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi6 – 1914
    Helixi29 – 4719
    Helixi50 – 556
    Helixi60 – 7718
    Helixi83 – 9917
    Helixi107 – 1104
    Helixi113 – 1197
    Helixi126 – 13914
    Helixi144 – 16421
    Turni165 – 1673
    Helixi177 – 18610
    Helixi188 – 1936
    Turni198 – 2003
    Turni203 – 2064
    Helixi207 – 23327
    Helixi243 – 2519
    Helixi255 – 27723
    Beta strandi278 – 2803
    Helixi282 – 30120
    Helixi303 – 3053
    Helixi308 – 3147
    Helixi320 – 33617
    Helixi340 – 3423
    Helixi348 – 3536

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1JFAX-ray2.50A/B1-374[»]
    1JFGX-ray2.50A/B1-374[»]
    1KIYX-ray2.40A/B1-374[»]
    1KIZX-ray2.60A/B1-374[»]
    1YJ4X-ray2.30A/B1-374[»]
    1YYQX-ray2.10A/B1-374[»]
    1YYRX-ray2.50A/B1-374[»]
    1YYSX-ray2.75A/B1-374[»]
    1YYTX-ray2.90A/B1-374[»]
    1YYUX-ray2.95A/B1-374[»]
    2AEKX-ray2.90A/B1-374[»]
    2AELX-ray2.50A/B1-374[»]
    2AETX-ray2.75A/B1-374[»]
    2PS4X-ray2.46A/B1-374[»]
    2PS5X-ray2.10A/B1-374[»]
    2PS6X-ray2.60A/B1-374[»]
    2PS7X-ray2.35A/B1-374[»]
    2PS8X-ray2.67A/B1-374[»]
    2Q9YX-ray2.85A/B1-374[»]
    2Q9ZX-ray2.95A/B1-374[»]
    ProteinModelPortaliP13513.
    SMRiP13513. Positions 1-354.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP13513.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the trichodiene synthase family.Curated

    Family and domain databases

    Gene3Di1.10.600.10. 1 hit.
    InterProiIPR008949. Terpenoid_synth.
    IPR010458. TRI5_ascomyc.
    IPR024652. Trichodiene_synth.
    [Graphical view]
    PfamiPF06330. TRI5. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001388. TRI5. 1 hit.
    SUPFAMiSSF48576. SSF48576. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P13513-1 [UniParc]FASTAAdd to Basket

    « Hide

    MENFPTEYFL NTTVRLLEYI RYRDSNYTRE ERIENLHYAY NKAAHHFAQP    50
    RQQQLLKVDP KRLQASLQTI VGMVVYSWAK VSKECMADLS IHYTYTLVLD 100
    DSKDDPYPTM VNYFDDLQAG REQAHPWWAL VNEHFPNVLR HFGPFCSLNL 150
    IRSTLDFFEG CWIEQYNFGG FPGSHDYPQF LRRMNGLGHC VGASLWPKEQ 200
    FNERSLFLEI TSAIAQMENW MVWVNDLMSF YKEFDDERDQ ISLVKNYVVS 250
    DEISLHEALE KLTQDTLHSS KQMVAVFSDK DPQVMDTIEC FMHGYVTWHL 300
    CDRRYRLSEI YEKVKEEKTE DAQKFCKFYE QAANVGAVSP SEWAYPPVAQ 350
    LANVRSKDVK EVQKPFLSSI ELVE 374
    Length:374
    Mass (Da):44,000
    Last modified:January 1, 1990 - v1
    Checksum:i189E8FC1663C3763
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF364179 Genomic DNA. Translation: AAD13657.1.
    PIRiJU0064. SYFUTP.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF364179 Genomic DNA. Translation: AAD13657.1 .
    PIRi JU0064. SYFUTP.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1JFA X-ray 2.50 A/B 1-374 [» ]
    1JFG X-ray 2.50 A/B 1-374 [» ]
    1KIY X-ray 2.40 A/B 1-374 [» ]
    1KIZ X-ray 2.60 A/B 1-374 [» ]
    1YJ4 X-ray 2.30 A/B 1-374 [» ]
    1YYQ X-ray 2.10 A/B 1-374 [» ]
    1YYR X-ray 2.50 A/B 1-374 [» ]
    1YYS X-ray 2.75 A/B 1-374 [» ]
    1YYT X-ray 2.90 A/B 1-374 [» ]
    1YYU X-ray 2.95 A/B 1-374 [» ]
    2AEK X-ray 2.90 A/B 1-374 [» ]
    2AEL X-ray 2.50 A/B 1-374 [» ]
    2AET X-ray 2.75 A/B 1-374 [» ]
    2PS4 X-ray 2.46 A/B 1-374 [» ]
    2PS5 X-ray 2.10 A/B 1-374 [» ]
    2PS6 X-ray 2.60 A/B 1-374 [» ]
    2PS7 X-ray 2.35 A/B 1-374 [» ]
    2PS8 X-ray 2.67 A/B 1-374 [» ]
    2Q9Y X-ray 2.85 A/B 1-374 [» ]
    2Q9Z X-ray 2.95 A/B 1-374 [» ]
    ProteinModelPortali P13513.
    SMRi P13513. Positions 1-354.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00267 .
    BRENDAi 4.2.3.6. 2364.

    Miscellaneous databases

    EvolutionaryTracei P13513.

    Family and domain databases

    Gene3Di 1.10.600.10. 1 hit.
    InterProi IPR008949. Terpenoid_synth.
    IPR010458. TRI5_ascomyc.
    IPR024652. Trichodiene_synth.
    [Graphical view ]
    Pfami PF06330. TRI5. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001388. TRI5. 1 hit.
    SUPFAMi SSF48576. SSF48576. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and nucleotide sequence of a sesquiterpene cyclase gene from the trichothecene-producing fungus Fusarium sporotrichioides."
      Hohn T.M., Beremand P.D.
      Gene 79:131-138(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
      Strain: NRRL 3299.
    2. "Structure of trichodiene synthase from Fusarium sporotrichioides provides mechanistic inferences on the terpene cyclization cascade."
      Rynkiewicz M.J., Cane D.E., Christianson D.W.
      Proc. Natl. Acad. Sci. U.S.A. 98:13543-13548(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).

    Entry informationi

    Entry nameiTRI5_FUSSP
    AccessioniPrimary (citable) accession number: P13513
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: January 1, 1990
    Last modified: October 1, 2014
    This is version 75 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Trichothecenes are sesquiterpenoid toxins that act by inhibiting protein biosynthesis.

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3