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P13513

- TRI5_FUSSP

UniProt

P13513 - TRI5_FUSSP

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Protein

Trichodiene synthase

Gene

TRI5

Organism
Fusarium sporotrichioides
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

TS is a member of the terpene cyclase group of enzymes. It catalyzes the isomerization and cyclization of farnesyl pyro-phosphate to form trichodiene, the first cyclic intermediate in the biosynthetic pathway for trichothecenes. It serves to branch trichothecene biosynthesis from the isoprenoid pathway.

Catalytic activityi

(2E,6E)-farnesyl diphosphate = trichodiene + diphosphate.

Pathwayi

GO - Molecular functioni

  1. trichodiene synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. sesquiterpenoid biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Enzyme and pathway databases

BRENDAi4.2.3.6. 2364.
UniPathwayiUPA00267.

Names & Taxonomyi

Protein namesi
Recommended name:
Trichodiene synthase (EC:4.2.3.6)
Alternative name(s):
Sesquiterpene cyclase
Short name:
TS
Gene namesi
Name:TRI5
Synonyms:TOX 5
OrganismiFusarium sporotrichioides
Taxonomic identifieri5514 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesHypocreomycetidaeHypocrealesNectriaceaeFusarium

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 374374Trichodiene synthasePRO_0000221584Add
BLAST

Structurei

Secondary structure

1
374
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi6 – 1914
Helixi29 – 4719
Helixi50 – 556
Helixi60 – 7718
Helixi83 – 9917
Helixi107 – 1104
Helixi113 – 1197
Helixi126 – 13914
Helixi144 – 16421
Turni165 – 1673
Helixi177 – 18610
Helixi188 – 1936
Turni198 – 2003
Turni203 – 2064
Helixi207 – 23327
Helixi243 – 2519
Helixi255 – 27723
Beta strandi278 – 2803
Helixi282 – 30120
Helixi303 – 3053
Helixi308 – 3147
Helixi320 – 33617
Helixi340 – 3423
Helixi348 – 3536

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1JFAX-ray2.50A/B1-374[»]
1JFGX-ray2.50A/B1-374[»]
1KIYX-ray2.40A/B1-374[»]
1KIZX-ray2.60A/B1-374[»]
1YJ4X-ray2.30A/B1-374[»]
1YYQX-ray2.10A/B1-374[»]
1YYRX-ray2.50A/B1-374[»]
1YYSX-ray2.75A/B1-374[»]
1YYTX-ray2.90A/B1-374[»]
1YYUX-ray2.95A/B1-374[»]
2AEKX-ray2.90A/B1-374[»]
2AELX-ray2.50A/B1-374[»]
2AETX-ray2.75A/B1-374[»]
2PS4X-ray2.46A/B1-374[»]
2PS5X-ray2.10A/B1-374[»]
2PS6X-ray2.60A/B1-374[»]
2PS7X-ray2.35A/B1-374[»]
2PS8X-ray2.67A/B1-374[»]
2Q9YX-ray2.85A/B1-374[»]
2Q9ZX-ray2.95A/B1-374[»]
ProteinModelPortaliP13513.
SMRiP13513. Positions 1-354.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP13513.

Family & Domainsi

Sequence similaritiesi

Belongs to the trichodiene synthase family.Curated

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
InterProiIPR008949. Terpenoid_synth.
IPR010458. TRI5_ascomyc.
IPR024652. Trichodiene_synth.
[Graphical view]
PfamiPF06330. TRI5. 1 hit.
[Graphical view]
PIRSFiPIRSF001388. TRI5. 1 hit.
SUPFAMiSSF48576. SSF48576. 1 hit.

Sequencei

Sequence statusi: Complete.

P13513-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MENFPTEYFL NTTVRLLEYI RYRDSNYTRE ERIENLHYAY NKAAHHFAQP
60 70 80 90 100
RQQQLLKVDP KRLQASLQTI VGMVVYSWAK VSKECMADLS IHYTYTLVLD
110 120 130 140 150
DSKDDPYPTM VNYFDDLQAG REQAHPWWAL VNEHFPNVLR HFGPFCSLNL
160 170 180 190 200
IRSTLDFFEG CWIEQYNFGG FPGSHDYPQF LRRMNGLGHC VGASLWPKEQ
210 220 230 240 250
FNERSLFLEI TSAIAQMENW MVWVNDLMSF YKEFDDERDQ ISLVKNYVVS
260 270 280 290 300
DEISLHEALE KLTQDTLHSS KQMVAVFSDK DPQVMDTIEC FMHGYVTWHL
310 320 330 340 350
CDRRYRLSEI YEKVKEEKTE DAQKFCKFYE QAANVGAVSP SEWAYPPVAQ
360 370
LANVRSKDVK EVQKPFLSSI ELVE
Length:374
Mass (Da):44,000
Last modified:January 1, 1990 - v1
Checksum:i189E8FC1663C3763
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF364179 Genomic DNA. Translation: AAD13657.1.
PIRiJU0064. SYFUTP.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF364179 Genomic DNA. Translation: AAD13657.1 .
PIRi JU0064. SYFUTP.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1JFA X-ray 2.50 A/B 1-374 [» ]
1JFG X-ray 2.50 A/B 1-374 [» ]
1KIY X-ray 2.40 A/B 1-374 [» ]
1KIZ X-ray 2.60 A/B 1-374 [» ]
1YJ4 X-ray 2.30 A/B 1-374 [» ]
1YYQ X-ray 2.10 A/B 1-374 [» ]
1YYR X-ray 2.50 A/B 1-374 [» ]
1YYS X-ray 2.75 A/B 1-374 [» ]
1YYT X-ray 2.90 A/B 1-374 [» ]
1YYU X-ray 2.95 A/B 1-374 [» ]
2AEK X-ray 2.90 A/B 1-374 [» ]
2AEL X-ray 2.50 A/B 1-374 [» ]
2AET X-ray 2.75 A/B 1-374 [» ]
2PS4 X-ray 2.46 A/B 1-374 [» ]
2PS5 X-ray 2.10 A/B 1-374 [» ]
2PS6 X-ray 2.60 A/B 1-374 [» ]
2PS7 X-ray 2.35 A/B 1-374 [» ]
2PS8 X-ray 2.67 A/B 1-374 [» ]
2Q9Y X-ray 2.85 A/B 1-374 [» ]
2Q9Z X-ray 2.95 A/B 1-374 [» ]
ProteinModelPortali P13513.
SMRi P13513. Positions 1-354.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00267 .
BRENDAi 4.2.3.6. 2364.

Miscellaneous databases

EvolutionaryTracei P13513.

Family and domain databases

Gene3Di 1.10.600.10. 1 hit.
InterProi IPR008949. Terpenoid_synth.
IPR010458. TRI5_ascomyc.
IPR024652. Trichodiene_synth.
[Graphical view ]
Pfami PF06330. TRI5. 1 hit.
[Graphical view ]
PIRSFi PIRSF001388. TRI5. 1 hit.
SUPFAMi SSF48576. SSF48576. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Isolation and nucleotide sequence of a sesquiterpene cyclase gene from the trichothecene-producing fungus Fusarium sporotrichioides."
    Hohn T.M., Beremand P.D.
    Gene 79:131-138(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
    Strain: NRRL 3299.
  2. "Structure of trichodiene synthase from Fusarium sporotrichioides provides mechanistic inferences on the terpene cyclization cascade."
    Rynkiewicz M.J., Cane D.E., Christianson D.W.
    Proc. Natl. Acad. Sci. U.S.A. 98:13543-13548(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).

Entry informationi

Entry nameiTRI5_FUSSP
AccessioniPrimary (citable) accession number: P13513
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: October 1, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Trichothecenes are sesquiterpenoid toxins that act by inhibiting protein biosynthesis.

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3