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Protein

Protein glp-1

Gene

glp-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the specification of the cell fates of the blastomeres, ABa and APa. Proper signaling by glp-1 induces ABa descendants to produce anterior pharyngeal cells, and APa descendants to adopt a different fate. Contributes to the establishment of the dorsal-ventral axis in early embryos (PubMed:8156602). Regulates germ cell mitotic proliferation probably by regulating MAP kinase phosphatase lip-1 expression (PubMed:22278922, PubMed:16319922). Plays a negative role in lifespan (PubMed:24332851).4 Publications

GO - Molecular functioni

GO - Biological processi

  • cell fate specification Source: WormBase
  • embryonic pattern specification Source: WormBase
  • maintenance of dauer Source: WormBase
  • nematode larval development Source: WormBase
  • Notch signaling pathway Source: InterPro
  • pharyngeal muscle development Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation

Enzyme and pathway databases

SignaLinkiP13508.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein glp-1
Gene namesi
Name:glp-1
Synonyms:emb-33
ORF Names:F02A9.6
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome III

Organism-specific databases

WormBaseiF02A9.6; CE00237; WBGene00001609; glp-1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini16 – 764749ExtracellularSequence analysisAdd
BLAST
Transmembranei765 – 78622HelicalSequence analysisAdd
BLAST
Topological domaini787 – 1295509CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • cytoplasm Source: WormBase
  • endomembrane system Source: WormBase
  • integral component of membrane Source: UniProtKB-KW
  • lateral plasma membrane Source: WormBase
  • plasma membrane Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

RNAi-mediated knockdown causes an increase in lifespan and a moderate increase in aak-2 phosphorylation (PubMed:24332851). RNAi-mediated knockdown in a rsks-1 mutant background reduces the formation of proximal germ cell tumors (PubMed:22278922).2 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi529 – 5291G → E in ar202; formation of proximal germ cell-derived tumors. 1 Publication
Mutagenesisi929 – 9291L → F in 2144; moderate increase in aak-2 phosphorylation. 1 Publication
Mutagenesisi974 – 9741R → C in 2141; reduced number of germline progenitors at the semi-permissive temperature (20 degrees Celsius). All germ cells are differentiated at the restrictive temperature(25 degrees Celsius). 1 Publication
Mutagenesisi1043 – 10431G → E in q224; at the restrictive temperature of 25 degrees Celsius, loss of interaction of lag-3 with lip-1 promoter. Loss of mpk-1 isoform b expression probably due to a lack of germline. 2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1515Sequence analysisAdd
BLAST
Chaini16 – 12951280Protein glp-1PRO_0000007594Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi23 ↔ 35By similarity
Disulfide bondi29 ↔ 46By similarity
Disulfide bondi48 ↔ 57By similarity
Disulfide bondi121 ↔ 131By similarity
Disulfide bondi126 ↔ 140By similarity
Disulfide bondi142 ↔ 151By similarity
Disulfide bondi158 ↔ 169By similarity
Disulfide bondi163 ↔ 178By similarity
Disulfide bondi180 ↔ 189By similarity
Disulfide bondi194 ↔ 206By similarity
Disulfide bondi201 ↔ 218By similarity
Disulfide bondi220 ↔ 229By similarity
Disulfide bondi236 ↔ 248By similarity
Disulfide bondi242 ↔ 257By similarity
Glycosylationi244 – 2441N-linked (GlcNAc...)Sequence analysis
Glycosylationi245 – 2451N-linked (GlcNAc...)Sequence analysis
Disulfide bondi259 ↔ 268By similarity
Disulfide bondi275 ↔ 286By similarity
Disulfide bondi280 ↔ 296By similarity
Disulfide bondi298 ↔ 307By similarity
Disulfide bondi329 ↔ 342By similarity
Glycosylationi333 – 3331N-linked (GlcNAc...)Sequence analysis
Disulfide bondi336 ↔ 347By similarity
Disulfide bondi349 ↔ 358By similarity
Disulfide bondi373 ↔ 384By similarity
Disulfide bondi378 ↔ 394By similarity
Glycosylationi381 – 3811N-linked (GlcNAc...)Sequence analysis
Disulfide bondi396 ↔ 405By similarity
Disulfide bondi411 ↔ 422By similarity
Disulfide bondi416 ↔ 431By similarity
Disulfide bondi433 ↔ 442By similarity
Disulfide bondi450 ↔ 461By similarity
Disulfide bondi455 ↔ 467By similarity
Disulfide bondi469 ↔ 478By similarity
Disulfide bondi496 ↔ 519By similarity
Disulfide bondi501 ↔ 514By similarity
Disulfide bondi510 ↔ 526By similarity
Disulfide bondi536 ↔ 560By similarity
Disulfide bondi542 ↔ 555By similarity
Disulfide bondi551 ↔ 567By similarity
Disulfide bondi582 ↔ 595By similarity
Disulfide bondi591 ↔ 607By similarity
Glycosylationi609 – 6091N-linked (GlcNAc...)Sequence analysis
Glycosylationi675 – 6751N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP13508.
PRIDEiP13508.

Expressioni

Developmental stagei

Acts on ABp development during 4-cell and 12-cell stages, and on ABa development during 12-cell and 28-cell stages.

Gene expression databases

BgeeiWBGene00001609.

Interactioni

Subunit structurei

Interacts with sel-10.1 Publication

Protein-protein interaction databases

BioGridi41485. 87 interactions.
DIPiDIP-26562N.
IntActiP13508. 13 interactions.
MINTiMINT-116124.
STRINGi6239.F02A9.6.

Structurei

3D structure databases

ProteinModelPortaliP13508.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini19 – 5840EGF-like 1PROSITE-ProRule annotationAdd
BLAST
Domaini117 – 15236EGF-like 2PROSITE-ProRule annotationAdd
BLAST
Domaini154 – 19037EGF-like 3PROSITE-ProRule annotationAdd
BLAST
Domaini190 – 23041EGF-like 4PROSITE-ProRule annotationAdd
BLAST
Domaini232 – 26938EGF-like 5; calcium-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini271 – 30838EGF-like 6PROSITE-ProRule annotationAdd
BLAST
Domaini316 – 35944EGF-like 7PROSITE-ProRule annotationAdd
BLAST
Domaini369 – 40638EGF-like 8PROSITE-ProRule annotationAdd
BLAST
Domaini407 – 44337EGF-like 9PROSITE-ProRule annotationAdd
BLAST
Domaini446 – 47934EGF-like 10PROSITE-ProRule annotationAdd
BLAST
Repeati496 – 53237LNR 1PROSITE-ProRule annotation1 PublicationAdd
BLAST
Repeati536 – 57742LNR 2PROSITE-ProRule annotation1 PublicationAdd
BLAST
Repeati581 – 61232LNR 3PROSITE-ProRule annotation1 PublicationAdd
BLAST
Repeati961 – 99030ANK 1PROSITE-ProRule annotation1 PublicationAdd
BLAST
Repeati994 – 102330ANK 2PROSITE-ProRule annotation1 PublicationAdd
BLAST
Repeati1030 – 106233ANK 3PROSITE-ProRule annotation1 PublicationAdd
BLAST
Repeati1074 – 110330ANK 4PROSITE-ProRule annotation1 PublicationAdd
BLAST
Repeati1107 – 113630ANK 5PROSITE-ProRule annotation1 PublicationAdd
BLAST

Sequence similaritiesi

Contains 5 ANK repeats.PROSITE-ProRule annotation
Contains 10 EGF-like domains.PROSITE-ProRule annotation
Contains 3 LNR (Lin/Notch) repeats.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, EGF-like domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IR7G. Eukaryota.
COG0666. LUCA.
GeneTreeiENSGT00810000125346.
HOGENOMiHOG000112741.
InParanoidiP13508.
OMAiARDCDEN.
OrthoDBiEOG091G01NU.
PhylomeDBiP13508.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR000800. Notch_dom.
IPR010660. Notch_NOD_dom.
IPR011656. Notch_NODP_dom.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF00008. EGF. 1 hit.
PF06816. NOD. 1 hit.
PF07684. NODP. 1 hit.
PF00066. Notch. 3 hits.
[Graphical view]
PRINTSiPR01452. LNOTCHREPEAT.
SMARTiSM00248. ANK. 5 hits.
SM00181. EGF. 11 hits.
SM00179. EGF_CA. 6 hits.
SM00004. NL. 3 hits.
SM01338. NOD. 1 hit.
SM01339. NODP. 1 hit.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF90193. SSF90193. 3 hits.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 3 hits.
PS00010. ASX_HYDROXYL. 2 hits.
PS00022. EGF_1. 10 hits.
PS01186. EGF_2. 8 hits.
PS50026. EGF_3. 10 hits.
PS01187. EGF_CA. 1 hit.
PS50258. LNR. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P13508-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVLLILLAF FAPIASQLMG GECGREGACS VNGKCYNGKL IETYWCRCKK
60 70 80 90 100
GFGGAFCERE CDLDCKRGEK CIYDVYGENP TCICQDCEDE TPPTERTQKG
110 120 130 140 150
CEEGYGGPDC KTPLFSGVNP CDSDPCNNGL CYPFYGGFQC ICNNGYGGSY
160 170 180 190 200
CEEGIDHCAQ NECAEGSTCV NSVYNYYCDC PIGKSGRYCE RTECALMGNI
210 220 230 240 250
CNHGRCIPNR DEDKNFRCVC DSGYEGEFCN KDKNECLIEE TCVNNSTCFN
260 270 280 290 300
LHGDFTCTCK PGYAGKYCEE AIDMCKDYVC QNDGYCAHDS NQMPICYCEQ
310 320 330 340 350
GFTGQRCEIE CPSGFGGIHC DLPLQRPHCS RSNGTCYNDG RCINGFCVCE
360 370 380 390 400
PDYIGDRCEI NRKDFKFPDI QSCKYNPCVN NATCIDLKNS GYSCHCPLGF
410 420 430 440 450
YGLNCEQHLL CTPTTCANGG TCEGVNGVIR CNCPNGFSGD YCEIKDRQLC
460 470 480 490 500
SRHPCKNGGV CKNTGYCECQ YGYTGPTCEE VLVIEKSKET VIRDLCEQRK
510 520 530 540 550
CMDLASNGIC NPECNLEECN FDGGDCSGGQ RPFSKCQYPA RCADQFANGV
560 570 580 590 600
CNQECNNEEC LYDGLDCQSE LFRCPAHIRK HCIERRGDGV CNLECSFIGC
610 620 630 640 650
GFDGGDCNNG TEAIILSDIR IKVQIDPIEF QATGGETLMQ ISANLRATVR
660 670 680 690 700
IQRDELGPLV FRWDGEHEME RVEMNSSKLE DQFVLSHHVR RYRQAVVTGI
710 720 730 740 750
VLYLEVEEIC KPEFCRFSTA QSVVDLIAAG LVKSDGRMSL GLPITEAMVA
760 770 780 790 800
VPKRNEIDEG WSRSQVILFA CIAFLAFGTV VAGVIAKNGP ERSRKRKMVN
810 820 830 840 850
ATVWMPPMES TNEKGRRNQS NHSSQCSLLD NSAYYHPNTK RHCSDYSTGY
860 870 880 890 900
NGEQYSQIYP QTLANGYPGD YNELNFDFQS ETFAPADLPA DEIPLHVQAA
910 920 930 940 950
GPDAITAPIT NESVNQVDSK YRRRVLHWLA ANVRGKPEDV ITTEAIRCLK
960 970 980 990 1000
AGADVNARDC DENTALMLAV RAHRVRLSVV LLREGANPTI FNNSERSALH
1010 1020 1030 1040 1050
EAVVNKDLRI LRHLLTDKRL LKEIDELDRN GMTALMLVAR ELGKHQVEMA
1060 1070 1080 1090 1100
ELLLSKGAKL DYDGAARKDS NKYKGRTALH YAAMHDNEEM VIMLVRRSSN
1110 1120 1130 1140 1150
KDKQDEDGRT PIMLAAKEGC EKTVQYLALN DASLGIVDSM DMTAAQVAEA
1160 1170 1180 1190 1200
SYHHELAAFL RQVANERHRN DIMRQQIVKS GHGAKSGRQT VKNIKRAGSR
1210 1220 1230 1240 1250
KTPTSAASSR ETNHLTPPPS DGSFSSPSPH YYPTTTSTPN RMETSPEYMF
1260 1270 1280 1290
NHEMAPPVNA MWYTTPPPYQ DPNYRHVPPN TAFQNAEQMN GSFYC
Length:1,295
Mass (Da):144,079
Last modified:January 1, 1990 - v1
Checksum:i422AAD0A2DEEF3B4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M25580 Genomic DNA. Translation: AAA28058.1.
Z19555, Z29116 Genomic DNA. Translation: CAA79620.1.
PIRiA32901.
RefSeqiNP_499014.1. NM_066613.4.
UniGeneiCel.6196.

Genome annotation databases

EnsemblMetazoaiF02A9.6; F02A9.6; WBGene00001609.
GeneIDi176286.
KEGGicel:CELE_F02A9.6.
UCSCiF02A9.6. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M25580 Genomic DNA. Translation: AAA28058.1.
Z19555, Z29116 Genomic DNA. Translation: CAA79620.1.
PIRiA32901.
RefSeqiNP_499014.1. NM_066613.4.
UniGeneiCel.6196.

3D structure databases

ProteinModelPortaliP13508.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi41485. 87 interactions.
DIPiDIP-26562N.
IntActiP13508. 13 interactions.
MINTiMINT-116124.
STRINGi6239.F02A9.6.

Proteomic databases

PaxDbiP13508.
PRIDEiP13508.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiF02A9.6; F02A9.6; WBGene00001609.
GeneIDi176286.
KEGGicel:CELE_F02A9.6.
UCSCiF02A9.6. c. elegans.

Organism-specific databases

CTDi176286.
WormBaseiF02A9.6; CE00237; WBGene00001609; glp-1.

Phylogenomic databases

eggNOGiENOG410IR7G. Eukaryota.
COG0666. LUCA.
GeneTreeiENSGT00810000125346.
HOGENOMiHOG000112741.
InParanoidiP13508.
OMAiARDCDEN.
OrthoDBiEOG091G01NU.
PhylomeDBiP13508.

Enzyme and pathway databases

SignaLinkiP13508.

Miscellaneous databases

PROiP13508.

Gene expression databases

BgeeiWBGene00001609.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR000800. Notch_dom.
IPR010660. Notch_NOD_dom.
IPR011656. Notch_NODP_dom.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF00008. EGF. 1 hit.
PF06816. NOD. 1 hit.
PF07684. NODP. 1 hit.
PF00066. Notch. 3 hits.
[Graphical view]
PRINTSiPR01452. LNOTCHREPEAT.
SMARTiSM00248. ANK. 5 hits.
SM00181. EGF. 11 hits.
SM00179. EGF_CA. 6 hits.
SM00004. NL. 3 hits.
SM01338. NOD. 1 hit.
SM01339. NODP. 1 hit.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF90193. SSF90193. 3 hits.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 3 hits.
PS00010. ASX_HYDROXYL. 2 hits.
PS00022. EGF_1. 10 hits.
PS01186. EGF_2. 8 hits.
PS50026. EGF_3. 10 hits.
PS01187. EGF_CA. 1 hit.
PS50258. LNR. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGLP1_CAEEL
AccessioniPrimary (citable) accession number: P13508
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: September 7, 2016
This is version 160 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.