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P13503

- SYLM_SACPA

UniProt

P13503 - SYLM_SACPA

Protein

Leucine--tRNA ligase, mitochondrial

Gene

NAM2

Organism
Saccharomyces paradoxus (Yeast) (Saccharomyces douglasii)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 89 (01 Oct 2014)
      Sequence version 1 (01 Jan 1990)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei649 – 6491ATPBy similarity

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. leucine-tRNA ligase activity Source: UniProtKB-EC

    GO - Biological processi

    1. leucyl-tRNA aminoacylation Source: InterPro

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Leucine--tRNA ligase, mitochondrial (EC:6.1.1.4)
    Alternative name(s):
    Leucyl-tRNA synthetase
    Short name:
    LeuRS
    Gene namesi
    Name:NAM2
    OrganismiSaccharomyces paradoxus (Yeast) (Saccharomyces douglasii)
    Taxonomic identifieri27291 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrial matrix Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 99MitochondrionBy similarity
    Chaini10 – 894885Leucine--tRNA ligase, mitochondrialPRO_0000035809Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP13503.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi56 – 6611"HIGH" regionAdd
    BLAST
    Motifi646 – 6505"KMSKS" region

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 3 hits.
    3.90.740.10. 1 hit.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002302. Leu-tRNA-ligase.
    IPR025709. Leu_tRNA-synth_edit.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
    PfamiPF00133. tRNA-synt_1. 3 hits.
    PF13603. tRNA-synt_1_2. 1 hit.
    [Graphical view]
    PRINTSiPR00985. TRNASYNTHLEU.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 2 hits.
    TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P13503-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLPRPSSRFL STQRGPGRAV KKLIAIGEKW KQKTTRGPPK QGTLNNRSKY    50
    ILCQFPYPSG VLHIGHLRVY VISDSLNRFY KQRGYNVIHP MGWDAFGLPA 100
    ENAAIERGIN PAIWTRDNIA KMKQQMESML ANFDWDREVT TCDPEYYKFT 150
    QWIFLKLFEN GLAYRKEAEI NWDPVDKTVL ANEQVDAQGR SWRSGAIVEK 200
    KQLKQWFLGI TKFAPKLRKH LNQLKDWPSN VKQMQKNWIG ESIGAELVFK 250
    VADSKFENLI VFTTRPETLF AVQYVALALD HPIVQKYSEV IPDLKEFLQK 300
    SDQLPSDTKE GFRLPDIKAV NPLTKEELPI FAAPYVISSY GTAPSAVMGC 350
    PGHDSRDFEF WQQNCPGEHI KTCIAPFFDD ASKTSEKERQ KIIDTVPFTS 400
    ADGILTKESG EYSGVFTAVA RKSIMGKLHS KGLSKNIIRY RIRDWLISRQ 450
    RYWGTPIPII HCDNCGPVPV PESDLPVKLP ELKGLDTKGN PLSTIDEFVN 500
    VACPSCGSPA KRETDTMDTF IDSSWYYFRF LDPKNTSKPF DREIASEHMP 550
    VDIYIGGVEH AILHLLYSRF IAKFLGSINA WDDPTGIFEP FRKLVTQGMV 600
    QGKTYVDPDS GKFLTPDELT FVKDPSDGNT TIIKSNGKIP MVSYEKMSKS 650
    KHNGADPNEC ILRHGADATR AHILFQSPIA DALNWDESKI VGIERWLQKV 700
    LCLTKNILGL EKNLAISKDY KTPTDLNDAE VKFHNDFQRF LKSITESFEV 750
    HLSLNTVISD YMKLTNLLES ALKKSEVRKE MMVQNLQKLV TIIYPAVPSI 800
    SEEAAELISS QMEWNQYRWP EVERTTESKF KKFQIVVNGR VKFMYTADKD 850
    FLKSGRDAVI ETLLKLPEGR MYLMNKKIKK FVMKYNVISF LFHK 894
    Length:894
    Mass (Da):102,194
    Last modified:January 1, 1990 - v1
    Checksum:iAE42C20193DDF107
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X12864 Genomic DNA. Translation: CAA31343.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X12864 Genomic DNA. Translation: CAA31343.1 .

    3D structure databases

    ProteinModelPortali P13503.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 3 hits.
    3.90.740.10. 1 hit.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002302. Leu-tRNA-ligase.
    IPR025709. Leu_tRNA-synth_edit.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF7. PTHR11946:SF7. 1 hit.
    Pfami PF00133. tRNA-synt_1. 3 hits.
    PF13603. tRNA-synt_1_2. 1 hit.
    [Graphical view ]
    PRINTSi PR00985. TRNASYNTHLEU.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 2 hits.
    TIGRFAMsi TIGR00396. leuS_bact. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Divergence of the mitochondrial leucyl tRNA synthetase genes in two closely related yeasts Saccharomyces cerevisiae and Saccharomyces douglasii: a paradigm of incipient evolution."
      Herbert C.J., Dujardin G., Labouesse M., Slonimski P.P.
      Mol. Gen. Genet. 213:297-309(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: HM300.
    2. "The NAM2 proteins from S. cerevisiae and S. douglasii are mitochondrial leucyl-tRNA synthetases, and are involved in mRNA splicing."
      Herbert C.J., Labouesse M., Dujardin G., Slonimski P.P.
      EMBO J. 7:473-483(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiSYLM_SACPA
    AccessioniPrimary (citable) accession number: P13503
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: January 1, 1990
    Last modified: October 1, 2014
    This is version 89 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3