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P13503 (SYLM_SACDO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase, mitochondrial

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:NAM2
OrganismSaccharomyces douglasii (Yeast)
Taxonomic identifier46617 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length894 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).

Subcellular location

Mitochondrion matrix.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentMitochondrion
   DomainTransit peptide
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

   Cellular_componentmitochondrial matrix

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 99Mitochondrion By similarity
Chain10 – 894885Leucine--tRNA ligase, mitochondrial
PRO_0000035809

Regions

Motif56 – 6611"HIGH" region
Motif646 – 6505"KMSKS" region

Sites

Binding site6491ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
P13503 [UniParc].

Last modified January 1, 1990. Version 1.
Checksum: AE42C20193DDF107

FASTA894102,194
        10         20         30         40         50         60 
MLPRPSSRFL STQRGPGRAV KKLIAIGEKW KQKTTRGPPK QGTLNNRSKY ILCQFPYPSG 

        70         80         90        100        110        120 
VLHIGHLRVY VISDSLNRFY KQRGYNVIHP MGWDAFGLPA ENAAIERGIN PAIWTRDNIA 

       130        140        150        160        170        180 
KMKQQMESML ANFDWDREVT TCDPEYYKFT QWIFLKLFEN GLAYRKEAEI NWDPVDKTVL 

       190        200        210        220        230        240 
ANEQVDAQGR SWRSGAIVEK KQLKQWFLGI TKFAPKLRKH LNQLKDWPSN VKQMQKNWIG 

       250        260        270        280        290        300 
ESIGAELVFK VADSKFENLI VFTTRPETLF AVQYVALALD HPIVQKYSEV IPDLKEFLQK 

       310        320        330        340        350        360 
SDQLPSDTKE GFRLPDIKAV NPLTKEELPI FAAPYVISSY GTAPSAVMGC PGHDSRDFEF 

       370        380        390        400        410        420 
WQQNCPGEHI KTCIAPFFDD ASKTSEKERQ KIIDTVPFTS ADGILTKESG EYSGVFTAVA 

       430        440        450        460        470        480 
RKSIMGKLHS KGLSKNIIRY RIRDWLISRQ RYWGTPIPII HCDNCGPVPV PESDLPVKLP 

       490        500        510        520        530        540 
ELKGLDTKGN PLSTIDEFVN VACPSCGSPA KRETDTMDTF IDSSWYYFRF LDPKNTSKPF 

       550        560        570        580        590        600 
DREIASEHMP VDIYIGGVEH AILHLLYSRF IAKFLGSINA WDDPTGIFEP FRKLVTQGMV 

       610        620        630        640        650        660 
QGKTYVDPDS GKFLTPDELT FVKDPSDGNT TIIKSNGKIP MVSYEKMSKS KHNGADPNEC 

       670        680        690        700        710        720 
ILRHGADATR AHILFQSPIA DALNWDESKI VGIERWLQKV LCLTKNILGL EKNLAISKDY 

       730        740        750        760        770        780 
KTPTDLNDAE VKFHNDFQRF LKSITESFEV HLSLNTVISD YMKLTNLLES ALKKSEVRKE 

       790        800        810        820        830        840 
MMVQNLQKLV TIIYPAVPSI SEEAAELISS QMEWNQYRWP EVERTTESKF KKFQIVVNGR 

       850        860        870        880        890 
VKFMYTADKD FLKSGRDAVI ETLLKLPEGR MYLMNKKIKK FVMKYNVISF LFHK 

« Hide

References

[1]"Divergence of the mitochondrial leucyl tRNA synthetase genes in two closely related yeasts Saccharomyces cerevisiae and Saccharomyces douglasii: a paradigm of incipient evolution."
Herbert C.J., Dujardin G., Labouesse M., Slonimski P.P.
Mol. Gen. Genet. 213:297-309(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: HM300.
[2]"The NAM2 proteins from S. cerevisiae and S. douglasii are mitochondrial leucyl-tRNA synthetases, and are involved in mRNA splicing."
Herbert C.J., Labouesse M., Dujardin G., Slonimski P.P.
EMBO J. 7:473-483(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X12864 Genomic DNA. Translation: CAA31343.1.

3D structure databases

ProteinModelPortalP13503.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYLM_SACDO
AccessionPrimary (citable) accession number: P13503
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: April 16, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries