Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P13503

- SYLM_SACPA

UniProt

P13503 - SYLM_SACPA

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Leucine--tRNA ligase, mitochondrial

Gene

NAM2

Organism
Saccharomyces paradoxus (Yeast) (Saccharomyces douglasii)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei649 – 6491ATPBy similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-KW
  3. leucine-tRNA ligase activity Source: UniProtKB-EC

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligase, mitochondrial (EC:6.1.1.4)
Alternative name(s):
Leucyl-tRNA synthetase
Short name:
LeuRS
Gene namesi
Name:NAM2
OrganismiSaccharomyces paradoxus (Yeast) (Saccharomyces douglasii)
Taxonomic identifieri27291 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Subcellular locationi

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 99MitochondrionBy similarity
Chaini10 – 894885Leucine--tRNA ligase, mitochondrialPRO_0000035809Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP13503.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi56 – 6611"HIGH" regionAdd
BLAST
Motifi646 – 6505"KMSKS" region

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P13503-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLPRPSSRFL STQRGPGRAV KKLIAIGEKW KQKTTRGPPK QGTLNNRSKY
60 70 80 90 100
ILCQFPYPSG VLHIGHLRVY VISDSLNRFY KQRGYNVIHP MGWDAFGLPA
110 120 130 140 150
ENAAIERGIN PAIWTRDNIA KMKQQMESML ANFDWDREVT TCDPEYYKFT
160 170 180 190 200
QWIFLKLFEN GLAYRKEAEI NWDPVDKTVL ANEQVDAQGR SWRSGAIVEK
210 220 230 240 250
KQLKQWFLGI TKFAPKLRKH LNQLKDWPSN VKQMQKNWIG ESIGAELVFK
260 270 280 290 300
VADSKFENLI VFTTRPETLF AVQYVALALD HPIVQKYSEV IPDLKEFLQK
310 320 330 340 350
SDQLPSDTKE GFRLPDIKAV NPLTKEELPI FAAPYVISSY GTAPSAVMGC
360 370 380 390 400
PGHDSRDFEF WQQNCPGEHI KTCIAPFFDD ASKTSEKERQ KIIDTVPFTS
410 420 430 440 450
ADGILTKESG EYSGVFTAVA RKSIMGKLHS KGLSKNIIRY RIRDWLISRQ
460 470 480 490 500
RYWGTPIPII HCDNCGPVPV PESDLPVKLP ELKGLDTKGN PLSTIDEFVN
510 520 530 540 550
VACPSCGSPA KRETDTMDTF IDSSWYYFRF LDPKNTSKPF DREIASEHMP
560 570 580 590 600
VDIYIGGVEH AILHLLYSRF IAKFLGSINA WDDPTGIFEP FRKLVTQGMV
610 620 630 640 650
QGKTYVDPDS GKFLTPDELT FVKDPSDGNT TIIKSNGKIP MVSYEKMSKS
660 670 680 690 700
KHNGADPNEC ILRHGADATR AHILFQSPIA DALNWDESKI VGIERWLQKV
710 720 730 740 750
LCLTKNILGL EKNLAISKDY KTPTDLNDAE VKFHNDFQRF LKSITESFEV
760 770 780 790 800
HLSLNTVISD YMKLTNLLES ALKKSEVRKE MMVQNLQKLV TIIYPAVPSI
810 820 830 840 850
SEEAAELISS QMEWNQYRWP EVERTTESKF KKFQIVVNGR VKFMYTADKD
860 870 880 890
FLKSGRDAVI ETLLKLPEGR MYLMNKKIKK FVMKYNVISF LFHK
Length:894
Mass (Da):102,194
Last modified:January 1, 1990 - v1
Checksum:iAE42C20193DDF107
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X12864 Genomic DNA. Translation: CAA31343.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X12864 Genomic DNA. Translation: CAA31343.1 .

3D structure databases

ProteinModelPortali P13503.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF7. PTHR11946:SF7. 1 hit.
Pfami PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view ]
PRINTSi PR00985. TRNASYNTHLEU.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsi TIGR00396. leuS_bact. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Divergence of the mitochondrial leucyl tRNA synthetase genes in two closely related yeasts Saccharomyces cerevisiae and Saccharomyces douglasii: a paradigm of incipient evolution."
    Herbert C.J., Dujardin G., Labouesse M., Slonimski P.P.
    Mol. Gen. Genet. 213:297-309(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: HM300.
  2. "The NAM2 proteins from S. cerevisiae and S. douglasii are mitochondrial leucyl-tRNA synthetases, and are involved in mRNA splicing."
    Herbert C.J., Labouesse M., Dujardin G., Slonimski P.P.
    EMBO J. 7:473-483(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiSYLM_SACPA
AccessioniPrimary (citable) accession number: P13503
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: October 29, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3