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P13503

- SYLM_SACPA

UniProt

P13503 - SYLM_SACPA

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Protein
Leucine--tRNA ligase, mitochondrial
Gene
NAM2
Organism
Saccharomyces paradoxus (Yeast) (Saccharomyces douglasii)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei649 – 6491ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. leucine-tRNA ligase activity Source: UniProtKB-EC

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligase, mitochondrial (EC:6.1.1.4)
Alternative name(s):
Leucyl-tRNA synthetase
Short name:
LeuRS
Gene namesi
Name:NAM2
OrganismiSaccharomyces paradoxus (Yeast) (Saccharomyces douglasii)
Taxonomic identifieri27291 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Subcellular locationi

GO - Cellular componenti

  1. mitochondrial matrix Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 99Mitochondrion By similarity
Chaini10 – 894885Leucine--tRNA ligase, mitochondrial
PRO_0000035809Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP13503.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi56 – 6611"HIGH" region
Add
BLAST
Motifi646 – 6505"KMSKS" region

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P13503-1 [UniParc]FASTAAdd to Basket

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MLPRPSSRFL STQRGPGRAV KKLIAIGEKW KQKTTRGPPK QGTLNNRSKY    50
ILCQFPYPSG VLHIGHLRVY VISDSLNRFY KQRGYNVIHP MGWDAFGLPA 100
ENAAIERGIN PAIWTRDNIA KMKQQMESML ANFDWDREVT TCDPEYYKFT 150
QWIFLKLFEN GLAYRKEAEI NWDPVDKTVL ANEQVDAQGR SWRSGAIVEK 200
KQLKQWFLGI TKFAPKLRKH LNQLKDWPSN VKQMQKNWIG ESIGAELVFK 250
VADSKFENLI VFTTRPETLF AVQYVALALD HPIVQKYSEV IPDLKEFLQK 300
SDQLPSDTKE GFRLPDIKAV NPLTKEELPI FAAPYVISSY GTAPSAVMGC 350
PGHDSRDFEF WQQNCPGEHI KTCIAPFFDD ASKTSEKERQ KIIDTVPFTS 400
ADGILTKESG EYSGVFTAVA RKSIMGKLHS KGLSKNIIRY RIRDWLISRQ 450
RYWGTPIPII HCDNCGPVPV PESDLPVKLP ELKGLDTKGN PLSTIDEFVN 500
VACPSCGSPA KRETDTMDTF IDSSWYYFRF LDPKNTSKPF DREIASEHMP 550
VDIYIGGVEH AILHLLYSRF IAKFLGSINA WDDPTGIFEP FRKLVTQGMV 600
QGKTYVDPDS GKFLTPDELT FVKDPSDGNT TIIKSNGKIP MVSYEKMSKS 650
KHNGADPNEC ILRHGADATR AHILFQSPIA DALNWDESKI VGIERWLQKV 700
LCLTKNILGL EKNLAISKDY KTPTDLNDAE VKFHNDFQRF LKSITESFEV 750
HLSLNTVISD YMKLTNLLES ALKKSEVRKE MMVQNLQKLV TIIYPAVPSI 800
SEEAAELISS QMEWNQYRWP EVERTTESKF KKFQIVVNGR VKFMYTADKD 850
FLKSGRDAVI ETLLKLPEGR MYLMNKKIKK FVMKYNVISF LFHK 894
Length:894
Mass (Da):102,194
Last modified:January 1, 1990 - v1
Checksum:iAE42C20193DDF107
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X12864 Genomic DNA. Translation: CAA31343.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X12864 Genomic DNA. Translation: CAA31343.1 .

3D structure databases

ProteinModelPortali P13503.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF7. PTHR11946:SF7. 1 hit.
Pfami PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view ]
PRINTSi PR00985. TRNASYNTHLEU.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsi TIGR00396. leuS_bact. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Divergence of the mitochondrial leucyl tRNA synthetase genes in two closely related yeasts Saccharomyces cerevisiae and Saccharomyces douglasii: a paradigm of incipient evolution."
    Herbert C.J., Dujardin G., Labouesse M., Slonimski P.P.
    Mol. Gen. Genet. 213:297-309(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: HM300.
  2. "The NAM2 proteins from S. cerevisiae and S. douglasii are mitochondrial leucyl-tRNA synthetases, and are involved in mRNA splicing."
    Herbert C.J., Labouesse M., Dujardin G., Slonimski P.P.
    EMBO J. 7:473-483(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiSYLM_SACPA
AccessioniPrimary (citable) accession number: P13503
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: July 9, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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