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Protein

Leucine--tRNA ligase, mitochondrial

Gene

NAM2

Organism
Saccharomyces paradoxus (Yeast) (Saccharomyces douglasii)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei649ATPBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligase, mitochondrial (EC:6.1.1.4)
Alternative name(s):
Leucyl-tRNA synthetase
Short name:
LeuRS
Gene namesi
Name:NAM2
OrganismiSaccharomyces paradoxus (Yeast) (Saccharomyces douglasii)
Taxonomic identifieri27291 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 9MitochondrionBy similarity9
ChainiPRO_000003580910 – 894Leucine--tRNA ligase, mitochondrialAdd BLAST885

Proteomic databases

PRIDEiP13503.

Structurei

3D structure databases

ProteinModelPortaliP13503.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi56 – 66"HIGH" regionAdd BLAST11
Motifi646 – 650"KMSKS" region5

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 3 hits.
PfamiPF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P13503-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLPRPSSRFL STQRGPGRAV KKLIAIGEKW KQKTTRGPPK QGTLNNRSKY
60 70 80 90 100
ILCQFPYPSG VLHIGHLRVY VISDSLNRFY KQRGYNVIHP MGWDAFGLPA
110 120 130 140 150
ENAAIERGIN PAIWTRDNIA KMKQQMESML ANFDWDREVT TCDPEYYKFT
160 170 180 190 200
QWIFLKLFEN GLAYRKEAEI NWDPVDKTVL ANEQVDAQGR SWRSGAIVEK
210 220 230 240 250
KQLKQWFLGI TKFAPKLRKH LNQLKDWPSN VKQMQKNWIG ESIGAELVFK
260 270 280 290 300
VADSKFENLI VFTTRPETLF AVQYVALALD HPIVQKYSEV IPDLKEFLQK
310 320 330 340 350
SDQLPSDTKE GFRLPDIKAV NPLTKEELPI FAAPYVISSY GTAPSAVMGC
360 370 380 390 400
PGHDSRDFEF WQQNCPGEHI KTCIAPFFDD ASKTSEKERQ KIIDTVPFTS
410 420 430 440 450
ADGILTKESG EYSGVFTAVA RKSIMGKLHS KGLSKNIIRY RIRDWLISRQ
460 470 480 490 500
RYWGTPIPII HCDNCGPVPV PESDLPVKLP ELKGLDTKGN PLSTIDEFVN
510 520 530 540 550
VACPSCGSPA KRETDTMDTF IDSSWYYFRF LDPKNTSKPF DREIASEHMP
560 570 580 590 600
VDIYIGGVEH AILHLLYSRF IAKFLGSINA WDDPTGIFEP FRKLVTQGMV
610 620 630 640 650
QGKTYVDPDS GKFLTPDELT FVKDPSDGNT TIIKSNGKIP MVSYEKMSKS
660 670 680 690 700
KHNGADPNEC ILRHGADATR AHILFQSPIA DALNWDESKI VGIERWLQKV
710 720 730 740 750
LCLTKNILGL EKNLAISKDY KTPTDLNDAE VKFHNDFQRF LKSITESFEV
760 770 780 790 800
HLSLNTVISD YMKLTNLLES ALKKSEVRKE MMVQNLQKLV TIIYPAVPSI
810 820 830 840 850
SEEAAELISS QMEWNQYRWP EVERTTESKF KKFQIVVNGR VKFMYTADKD
860 870 880 890
FLKSGRDAVI ETLLKLPEGR MYLMNKKIKK FVMKYNVISF LFHK
Length:894
Mass (Da):102,194
Last modified:January 1, 1990 - v1
Checksum:iAE42C20193DDF107
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X12864 Genomic DNA. Translation: CAA31343.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X12864 Genomic DNA. Translation: CAA31343.1.

3D structure databases

ProteinModelPortaliP13503.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP13503.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 3 hits.
PfamiPF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYLM_SACPA
AccessioniPrimary (citable) accession number: P13503
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: October 5, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.