P13502 (SYI_ENTAE) Reviewed, UniProtKB/Swiss-Prot
Last modified February 19, 2014. Version 73. History...
Names and origin
|Protein names||Recommended name:|
|Organism||Enterobacter aerogenes (Aerobacter aerogenes)|
|Taxonomic identifier||548 [NCBI]|
|Taxonomic lineage||Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Enterobacter|
|Sequence length||92 AA.|
|Protein existence||Inferred from homology|
General annotation (Comments)
Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.
ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).
Binds 1 zinc ion per subunit By similarity.
Monomer By similarity.
Cytoplasm By similarity.
IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.
|Biological process||Protein biosynthesis|
|Molecular function||Aminoacyl-tRNA synthetase|
|Gene Ontology (GO)|
|Biological_process||tRNA aminoacylation for protein translation|
Inferred from electronic annotation. Source: InterPro
Inferred from electronic annotation. Source: UniProtKB-SubCell
Inferred from electronic annotation. Source: UniProtKB-KWisoleucine-tRNA ligase activity
Inferred from electronic annotation. Source: UniProtKB-ECmetal ion binding
Inferred from electronic annotation. Source: UniProtKB-KW
|Complete GO annotation...|
Sequence annotation (Features)
|Feature key||Position(s)||Length||Description||Graphical view||Feature identifier|
|Chain||‹1 – 92||›92||Isoleucine--tRNA ligase||PRO_0000098381|
|Metal binding||55||1||Zinc By similarity|
|Metal binding||58||1||Zinc By similarity|
|Metal binding||75||1||Zinc By similarity|
|Metal binding||78||1||Zinc By similarity|
|M26713 Genomic DNA. Translation: AAA24803.1.|
3D structure databases
Protocols and materials databases
Family and domain databases
|InterPro||IPR009080. tRNAsynth_1a_anticodon-bd. |
|Pfam||PF06827. zf-FPG_IleRS. 1 hit. |
|SUPFAM||SSF47323. SSF47323. 1 hit. |
|Accession||Primary (citable) accession number: P13502|
|Entry status||Reviewed (UniProtKB/Swiss-Prot)|
|Annotation program||Prokaryotic Protein Annotation Program|