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P13502

- SYI_ENTAE

UniProt

P13502 - SYI_ENTAE

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Enterobacter aerogenes (Aerobacter aerogenes)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.By similarity

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).

Cofactori

Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi55 – 551ZincBy similarity
Metal bindingi58 – 581ZincBy similarity
Metal bindingi75 – 751ZincBy similarity
Metal bindingi78 – 781ZincBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. isoleucine-tRNA ligase activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. tRNA aminoacylation for protein translation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
OrganismiEnterobacter aerogenes (Aerobacter aerogenes)
Taxonomic identifieri548 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEnterobacter

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini‹1 – 92›92Isoleucine--tRNA ligasePRO_0000098381Add
BLAST

Interactioni

Subunit structurei

Monomer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP13502.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.By similarity

Sequence similaritiesi

Family and domain databases

InterProiIPR009080. tRNAsynth_1a_anticodon-bd.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PfamiPF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.

Sequencei

Sequence statusi: Fragment.

P13502-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
DLAAKLNALG EELRFVLLTS GANVADYAQA PADAWQSDLL KGLKVVLSKA
60 70 80 90
EGEKCPRCWH YTSDVGKVAE HAEICGRCVS NVAGDGEQRK FA
Length:92
Mass (Da):9,862
Last modified:January 1, 1990 - v1
Checksum:i9B17CEBD56C08895
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M26713 Genomic DNA. Translation: AAA24803.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M26713 Genomic DNA. Translation: AAA24803.1 .

3D structure databases

ProteinModelPortali P13502.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR009080. tRNAsynth_1a_anticodon-bd.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
Pfami PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
SUPFAMi SSF47323. SSF47323. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Cloning and nucleotide sequence of the Enterobacter aerogenes signal peptidase II (lsp) gene."
    Isaki L., Kawakami M., Beers R., Hom R., Wu H.C.
    J. Bacteriol. 172:469-472(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiSYI_ENTAE
AccessioniPrimary (citable) accession number: P13502
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: October 29, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3