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P13500

- CCL2_HUMAN

UniProt

P13500 - CCL2_HUMAN

Protein

C-C motif chemokine 2

Gene

CCL2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei31 – 311Involved in dimerization
    Sitei36 – 361Involved in dimerization, receptor binding and signaling
    Sitei41 – 411Involved in GAG binding
    Sitei42 – 421Involved in GAG binding
    Sitei47 – 471Involved in GAG binding and receptor binding
    Sitei58 – 581Involved in dimerization
    Sitei61 – 611Involved in dimerization
    Sitei72 – 721Involved in GAG binding and receptor binding
    Sitei81 – 811Involved in GAG binding
    Sitei89 – 891Involved in GAG binding

    GO - Molecular functioni

    1. CCR2 chemokine receptor binding Source: BHF-UCL
    2. chemokine activity Source: ProtInc
    3. heparin binding Source: Ensembl
    4. protein kinase activity Source: ProtInc
    5. receptor binding Source: ProtInc

    GO - Biological processi

    1. activation of signaling protein activity involved in unfolded protein response Source: Reactome
    2. aging Source: Ensembl
    3. angiogenesis Source: BHF-UCL
    4. astrocyte cell migration Source: BHF-UCL
    5. cell adhesion Source: ProtInc
    6. cell surface receptor signaling pathway Source: ProtInc
    7. cellular calcium ion homeostasis Source: Ensembl
    8. cellular homeostasis Source: BHF-UCL
    9. cellular protein metabolic process Source: Reactome
    10. cellular response to fibroblast growth factor stimulus Source: UniProtKB
    11. cellular response to interferon-gamma Source: UniProtKB
    12. cellular response to interleukin-1 Source: UniProtKB
    13. cellular response to lipopolysaccharide Source: BHF-UCL
    14. cellular response to organic cyclic compound Source: UniProtKB
    15. cellular response to tumor necrosis factor Source: UniProtKB
    16. chemokine-mediated signaling pathway Source: Ensembl
    17. chemotaxis Source: ProtInc
    18. cytokine-mediated signaling pathway Source: BHF-UCL
    19. cytoskeleton organization Source: UniProtKB
    20. endoplasmic reticulum unfolded protein response Source: Reactome
    21. G-protein coupled receptor signaling pathway Source: ProtInc
    22. G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger Source: ProtInc
    23. helper T cell extravasation Source: BHF-UCL
    24. humoral immune response Source: ProtInc
    25. inflammatory response Source: UniProtKB
    26. JAK-STAT cascade Source: ProtInc
    27. lipopolysaccharide-mediated signaling pathway Source: UniProtKB
    28. lymphocyte chemotaxis Source: Ensembl
    29. macrophage chemotaxis Source: BHF-UCL
    30. MAPK cascade Source: UniProtKB
    31. maternal process involved in parturition Source: Ensembl
    32. monocyte chemotaxis Source: BHF-UCL
    33. negative regulation of angiogenesis Source: Ensembl
    34. negative regulation of glial cell apoptotic process Source: UniProtKB
    35. negative regulation of natural killer cell chemotaxis Source: UniProtKB
    36. negative regulation of neuron apoptotic process Source: UniProtKB
    37. neutrophil chemotaxis Source: Ensembl
    38. organ morphogenesis Source: ProtInc
    39. organ regeneration Source: Ensembl
    40. positive regulation of apoptotic cell clearance Source: BHF-UCL
    41. positive regulation of calcium ion import Source: BHF-UCL
    42. positive regulation of endothelial cell proliferation Source: Ensembl
    43. positive regulation of immune complex clearance by monocytes and macrophages Source: Ensembl
    44. positive regulation of macrophage chemotaxis Source: Ensembl
    45. positive regulation of nitric-oxide synthase biosynthetic process Source: BHF-UCL
    46. positive regulation of synaptic transmission Source: Ensembl
    47. positive regulation of T cell activation Source: BHF-UCL
    48. protein kinase B signaling Source: UniProtKB
    49. protein phosphorylation Source: ProtInc
    50. regulation of cell shape Source: UniProtKB
    51. regulation of vascular endothelial growth factor production Source: Ensembl
    52. response to activity Source: Ensembl
    53. response to amino acid Source: Ensembl
    54. response to antibiotic Source: Ensembl
    55. response to bacterium Source: BHF-UCL
    56. response to drug Source: Ensembl
    57. response to ethanol Source: Ensembl
    58. response to gamma radiation Source: Ensembl
    59. response to glucocorticoid Source: Ensembl
    60. response to heat Source: Ensembl
    61. response to hypoxia Source: Ensembl
    62. response to mechanical stimulus Source: Ensembl
    63. response to progesterone Source: Ensembl
    64. response to vitamin B3 Source: Ensembl
    65. signal transduction Source: ProtInc
    66. transforming growth factor beta receptor signaling pathway Source: Ensembl
    67. vascular endothelial growth factor receptor signaling pathway Source: Ensembl
    68. viral genome replication Source: ProtInc

    Keywords - Molecular functioni

    Cytokine

    Keywords - Biological processi

    Chemotaxis, Inflammatory response

    Enzyme and pathway databases

    ReactomeiREACT_15344. Chemokine receptors bind chemokines.
    REACT_18355. ATF4 activates genes.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    C-C motif chemokine 2
    Alternative name(s):
    HC11
    Monocyte chemoattractant protein 1
    Monocyte chemotactic and activating factor
    Short name:
    MCAF
    Monocyte chemotactic protein 1
    Short name:
    MCP-1
    Monocyte secretory protein JE
    Small-inducible cytokine A2
    Gene namesi
    Name:CCL2
    Synonyms:MCP1, SCYA2
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 17

    Organism-specific databases

    HGNCiHGNC:10618. CCL2.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: Ensembl
    2. extracellular region Source: BHF-UCL
    3. extracellular space Source: ProtInc
    4. neuronal cell body Source: Ensembl

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi24 – 9168Missing: 83% reduction in activity. Add
    BLAST
    Mutagenesisi24 – 8562Missing: 90% reduction in activity. Add
    BLAST
    Mutagenesisi24 – 241Missing: Loss of activity.
    Mutagenesisi25 – 317Missing: Loss of signaling.
    Mutagenesisi26 – 261D → A: Reduction in activity. 1 Publication
    Mutagenesisi28 – 281I → A: Slight reduction in activity. 1 Publication
    Mutagenesisi29 – 291N → A: 50% reduction in activity. 1 Publication
    Mutagenesisi31 – 311P → A: Loss of dimerization; slight reduction of activity. 2 Publications
    Mutagenesisi32 – 321V → A: Slight reduction in activity. 2 Publications
    Mutagenesisi32 – 321V → E: Slight reduction in affinity. 2 Publications
    Mutagenesisi33 – 331T → A: Slight reduction in activity. 2 Publications
    Mutagenesisi33 – 331T → E: Slight reduction in affinity. 2 Publications
    Mutagenesisi36 – 361Y → A: Loss of activity. 1 Publication
    Mutagenesisi47 – 471R → F: 95% reduction in activity; strong reduction of receptor binding.
    Mutagenesisi50 – 501S → Q: 40% reduction in activity.
    Mutagenesisi51 – 511Y → D: Loss of activity.
    Mutagenesisi53 – 531R → L: Loss of activity.
    Mutagenesisi79 – 791K → A: No effect on heparin binding.
    Mutagenesisi81 – 811K → A: Strongly reduces heparin binding. 1 Publication
    Mutagenesisi89 – 891H → A: Strongly reduces heparin binding. 1 Publication
    Mutagenesisi91 – 911D → L: 90% reduction in activity.
    Mutagenesisi95 – 995Missing: No effect on heparin binding.

    Organism-specific databases

    MIMi607948. phenotype.
    PharmGKBiPA130413151.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 23231 PublicationAdd
    BLAST
    Chaini24 – 9976C-C motif chemokine 2PRO_0000005146Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei24 – 241Pyrrolidone carboxylic acid2 Publications
    Disulfide bondi34 ↔ 59
    Disulfide bondi35 ↔ 75
    Glycosylationi37 – 371N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Pyrrolidone carboxylic acid

    Proteomic databases

    PaxDbiP13500.
    PRIDEiP13500.

    Miscellaneous databases

    PMAP-CutDBP13500.

    Expressioni

    Inductioni

    Up-regulated upon hypertonic conditions.1 Publication

    Gene expression databases

    ArrayExpressiP13500.
    BgeeiP13500.
    CleanExiHS_CCL2.
    GenevestigatoriP13500.

    Organism-specific databases

    HPAiCAB013676.
    HPA019163.

    Interactioni

    Subunit structurei

    Monomer or homodimer; in equilibrium. Binds to CCR2 and CCR4. Is tethered on endothelial cells by glycosaminoglycan (GAG) side chains of proteoglycans.1 Publication

    Protein-protein interaction databases

    BioGridi112251. 15 interactions.
    DIPiDIP-5838N.
    IntActiP13500. 1 interaction.
    MINTiMINT-103269.
    STRINGi9606.ENSP00000225831.

    Structurei

    Secondary structure

    1
    99
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi25 – 295
    Beta strandi32 – 343
    Helixi45 – 473
    Beta strandi48 – 547
    Beta strandi59 – 613
    Beta strandi63 – 686
    Beta strandi73 – 764
    Helixi81 – 9212
    Beta strandi93 – 953

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1DOKX-ray1.85A/B24-99[»]
    1DOLX-ray2.40A24-99[»]
    1DOMNMR-A/B24-99[»]
    1DONNMR-A/B24-99[»]
    1MCAmodel-A/B24-96[»]
    1ML0X-ray2.80D24-99[»]
    2BDNX-ray2.53A24-99[»]
    2NZ1X-ray2.50D/E/Y24-99[»]
    3IFDX-ray1.90A24-99[»]
    4DN4X-ray2.80M24-99[»]
    ProteinModelPortaliP13500.
    SMRiP13500. Positions 24-94.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP13500.

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG46819.
    HOGENOMiHOG000036686.
    HOVERGENiHBG017871.
    InParanoidiP13500.
    KOiK14624.
    OMAiNKEICAD.
    PhylomeDBiP13500.
    TreeFamiTF334888.

    Family and domain databases

    InterProiIPR000827. Chemokine_CC_CS.
    IPR001811. Chemokine_IL8-like_dom.
    [Graphical view]
    PfamiPF00048. IL8. 1 hit.
    [Graphical view]
    SMARTiSM00199. SCY. 1 hit.
    [Graphical view]
    SUPFAMiSSF54117. SSF54117. 1 hit.
    PROSITEiPS00472. SMALL_CYTOKINES_CC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P13500-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKVSAALLCL LLIAATFIPQ GLAQPDAINA PVTCCYNFTN RKISVQRLAS   50
    YRRITSSKCP KEAVIFKTIV AKEICADPKQ KWVQDSMDHL DKQTQTPKT 99
    Length:99
    Mass (Da):11,025
    Last modified:January 1, 1990 - v1
    Checksum:i45EC72361435302F
    GO

    Polymorphismi

    Genetic variations in CCL2 determine Mycobacterium tuberculosis susceptibility [MIMi:607948].

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M24545 mRNA. Translation: AAA18164.1.
    M28225, M28223, M28224 Genomic DNA. Translation: AAA60308.1.
    M28226 mRNA. Translation: AAA60309.1.
    M31626, M30816, M31625 Genomic DNA. Translation: AAA36330.1.
    X14768 mRNA. Translation: CAA32876.1.
    M37719 Genomic DNA. Translation: AAA18102.1.
    S71513 mRNA. Translation: AAB20651.1.
    S69738 mRNA. Translation: AAB29926.1.
    Y18933 Genomic DNA. Translation: CAC14049.1.
    A17786 mRNA. Translation: CAA01352.1.
    BT007329 mRNA. Translation: AAP35993.1.
    AF519531 Genomic DNA. Translation: AAM54046.1.
    AK311960 mRNA. Translation: BAG34900.1.
    CH471147 Genomic DNA. Translation: EAW80212.1.
    BC009716 mRNA. Translation: AAH09716.1.
    CCDSiCCDS11277.1.
    PIRiA35474. A60299.
    RefSeqiNP_002973.1. NM_002982.3.
    UniGeneiHs.303649.

    Genome annotation databases

    EnsembliENST00000225831; ENSP00000225831; ENSG00000108691.
    GeneIDi6347.
    KEGGihsa:6347.
    UCSCiuc002hhy.3. human.

    Polymorphism databases

    DMDMi126842.

    Cross-referencesi

    Web resourcesi

    Wikipedia

    CCL2 entry

    SeattleSNPs

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M24545 mRNA. Translation: AAA18164.1 .
    M28225 , M28223 , M28224 Genomic DNA. Translation: AAA60308.1 .
    M28226 mRNA. Translation: AAA60309.1 .
    M31626 , M30816 , M31625 Genomic DNA. Translation: AAA36330.1 .
    X14768 mRNA. Translation: CAA32876.1 .
    M37719 Genomic DNA. Translation: AAA18102.1 .
    S71513 mRNA. Translation: AAB20651.1 .
    S69738 mRNA. Translation: AAB29926.1 .
    Y18933 Genomic DNA. Translation: CAC14049.1 .
    A17786 mRNA. Translation: CAA01352.1 .
    BT007329 mRNA. Translation: AAP35993.1 .
    AF519531 Genomic DNA. Translation: AAM54046.1 .
    AK311960 mRNA. Translation: BAG34900.1 .
    CH471147 Genomic DNA. Translation: EAW80212.1 .
    BC009716 mRNA. Translation: AAH09716.1 .
    CCDSi CCDS11277.1.
    PIRi A35474. A60299.
    RefSeqi NP_002973.1. NM_002982.3.
    UniGenei Hs.303649.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1DOK X-ray 1.85 A/B 24-99 [» ]
    1DOL X-ray 2.40 A 24-99 [» ]
    1DOM NMR - A/B 24-99 [» ]
    1DON NMR - A/B 24-99 [» ]
    1MCA model - A/B 24-96 [» ]
    1ML0 X-ray 2.80 D 24-99 [» ]
    2BDN X-ray 2.53 A 24-99 [» ]
    2NZ1 X-ray 2.50 D/E/Y 24-99 [» ]
    3IFD X-ray 1.90 A 24-99 [» ]
    4DN4 X-ray 2.80 M 24-99 [» ]
    ProteinModelPortali P13500.
    SMRi P13500. Positions 24-94.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 112251. 15 interactions.
    DIPi DIP-5838N.
    IntActi P13500. 1 interaction.
    MINTi MINT-103269.
    STRINGi 9606.ENSP00000225831.

    Chemistry

    ChEMBLi CHEMBL1649052.
    DrugBanki DB01076. Atorvastatin.
    DB01406. Danazol.
    DB01055. Mimosine.
    DB00641. Simvastatin.

    Polymorphism databases

    DMDMi 126842.

    Proteomic databases

    PaxDbi P13500.
    PRIDEi P13500.

    Protocols and materials databases

    DNASUi 6347.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000225831 ; ENSP00000225831 ; ENSG00000108691 .
    GeneIDi 6347.
    KEGGi hsa:6347.
    UCSCi uc002hhy.3. human.

    Organism-specific databases

    CTDi 6347.
    GeneCardsi GC17P032582.
    HGNCi HGNC:10618. CCL2.
    HPAi CAB013676.
    HPA019163.
    MIMi 158105. gene.
    607948. phenotype.
    neXtProti NX_P13500.
    PharmGKBi PA130413151.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG46819.
    HOGENOMi HOG000036686.
    HOVERGENi HBG017871.
    InParanoidi P13500.
    KOi K14624.
    OMAi NKEICAD.
    PhylomeDBi P13500.
    TreeFami TF334888.

    Enzyme and pathway databases

    Reactomei REACT_15344. Chemokine receptors bind chemokines.
    REACT_18355. ATF4 activates genes.

    Miscellaneous databases

    EvolutionaryTracei P13500.
    GeneWikii CCL2.
    GenomeRNAii 6347.
    NextBioi 24660.
    PMAP-CutDB P13500.
    PROi P13500.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P13500.
    Bgeei P13500.
    CleanExi HS_CCL2.
    Genevestigatori P13500.

    Family and domain databases

    InterProi IPR000827. Chemokine_CC_CS.
    IPR001811. Chemokine_IL8-like_dom.
    [Graphical view ]
    Pfami PF00048. IL8. 1 hit.
    [Graphical view ]
    SMARTi SM00199. SCY. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54117. SSF54117. 1 hit.
    PROSITEi PS00472. SMALL_CYTOKINES_CC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequencing of the cDNA for human monocyte chemotactic and activating factor (MCAF)."
      Furutani Y., Nomura H., Notake M., Oyamada Y., Fukui T., Yamada M., Larsen C.G., Oppenheim J.J., Matsushima K.
      Biochem. Biophys. Res. Commun. 159:249-255(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The human homolog of the JE gene encodes a monocyte secretory protein."
      Rollins B.J., Stier P., Ernst T., Wong G.G.
      Mol. Cell. Biol. 9:4687-4695(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    3. "Human monocyte chemoattractant protein-1 (MCP-1). Full-length cDNA cloning, expression in mitogen-stimulated blood mononuclear leukocytes, and sequence similarity to mouse competence gene JE."
      Yoshimura T., Yuhki N., Moore S.K., Appella E., Lerman M.I., Leonard E.J.
      FEBS Lett. 244:487-493(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Glial tumor.
    4. "Cloning and expression of a gamma-interferon-inducible gene in monocytes: a new member of a cytokine gene family."
      Chang H.C., Hsu F., Freeman G.J., Griffin J.D., Reinherz E.L.
      Int. Immunol. 1:388-397(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    5. "Structure of human monocyte chemotactic protein gene and its regulation by TPA."
      Shyy Y.J., Li Y.S., Kolattukudy P.E.
      Biochem. Biophys. Res. Commun. 169:346-351(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    6. "Human monocyte chemoattractant protein-1 (MCP-1)."
      Yoshimura T., Leonard E.J.
      Adv. Exp. Med. Biol. 305:47-56(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    7. "The expression of monocyte chemotactic protein (MCP-1) in human vascular endothelium in vitro and in vivo."
      Li Y.S., Shyy Y.J., Wright J.G., Valente A.J., Cornhill J.F., Kolattukudy P.E.
      Mol. Cell. Biochem. 126:61-68(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    8. "Differential transcriptional regulation of the monocyte-chemoattractant protein-1 (MCP-1) gene in tumorigenic and non-tumorigenic HPV 18 positive cells: the role of the chromatin structure and AP-1 composition."
      Finzer P., Soto U., Delius H., Patzelt A., Poustka A., Coy J.F., zur Hausen H., Roesl F.
      Oncogene 19:3235-3244(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    9. "Protein with cytokine activity, recombinant DNA, expression vector and hosts for obtaining it."
      Caput D., Ferrara P., Miloux B., Minty A., Vita N.
      Patent number EP0488900, 03-JUN-1992
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    10. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
      Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
      Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    11. SeattleSNPs variation discovery resource
      Submitted (JUN-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    12. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Urinary bladder.
    13. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    14. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Pancreas.
    15. "Complete amino acid sequence of a human monocyte chemoattractant, a putative mediator of cellular immune reactions."
      Robinson E.A., Yoshimura T., Leonard E.J., Tanaka S., Griffin P.R., Shabanowitz J., Hunt D.F., Appella E.
      Proc. Natl. Acad. Sci. U.S.A. 86:1850-1854(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 24-99, PYROGLUTAMATE FORMATION AT GLN-24.
    16. "Identification of the monocyte chemotactic protein from human osteosarcoma cells and monocytes: detection of a novel N-terminally processed form."
      Decock B., Conings R., Lenaerts J.-P., Biliau A., van Damme J.
      Biochem. Biophys. Res. Commun. 167:904-909(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 29-53 AND 82-92.
    17. "Assignment of the human small inducible cytokine A2 gene, SCYA2 (encoding JE or MCP-1), to 17q11.2-12: evolutionary relatedness of cytokines clustered at the same locus."
      Rollins B.J., Morton C.C., Ledbetter D.H., Eddy R.L. Jr., Shows T.B.
      Genomics 10:489-492(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE STRUCTURE.
    18. "Structure/activity analysis of human monocyte chemoattractant protein-1 (MCP-1) by mutagenesis. Identification of a mutated protein that inhibits MCP-1-mediated monocyte chemotaxis."
      Zhang Y.J., Rutledge B.J., Rollins B.J.
      J. Biol. Chem. 269:15918-15924(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS.
    19. "Deletion of the NH2-terminal residue converts monocyte chemotactic protein 1 from an activator of basophil mediator release to an eosinophil chemoattractant."
      Weber M., Uguccioni M., Baggiolini M., Clark-Lewis I., Dahinden C.A.
      J. Exp. Med. 183:681-685(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: EFFECT OF DELETION OF N-TERMINAL RESIDUES.
    20. "Structural characterization of a monomeric chemokine: monocyte chemoattractant protein-3."
      Kim K.-S., Rajarathnam K., Clark-Lewis I., Sykes B.D.
      FEBS Lett. 395:277-282(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT.
    21. "Lysine 58 and histidine 66 at the C-terminal alpha-helix of monocyte chemoattractant protein-1 are essential for glycosaminoglycan binding."
      Chakravarty L., Rogers L., Quach T., Breckenridge S., Kolattukudy P.E.
      J. Biol. Chem. 273:29641-29647(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: GAG BINDING SITES LYS-81 AND HIS-89, MUTAGENESIS OF LYS-81; HIS-89 AND 95-GLN--THR-99.
    22. "Monomeric monocyte chemoattractant protein-1 (MCP-1) binds and activates the MCP-1 receptor CCR2B."
      Paavola C.D., Hemmerich S., Grunberger D., Polsky I., Bloom A., Freedman R., Mulkins M., Bhakta S., McCarley D., Wiesent L., Wong B., Jarnagin K., Handel T.M.
      J. Biol. Chem. 273:33157-33165(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: HOMODIMERIZATION, MUTAGENESIS OF PRO-31; VAL-32; THR-33 AND TYR-36.
    23. "Identification of surface residues of the monocyte chemotactic protein 1 that affect signaling through the receptor CCR2."
      Jarnagin K., Grunberger D., Mulkins M., Wong B., Hemmerich S., Paavola C., Bloom A., Bhakta S., Diehl F., Freedman R., McCarley D., Polsky I., Ping-Tsou A., Kosaka A., Handel T.M.
      Biochemistry 38:16167-16177(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF 25-PRO--PRO-31; ASP-26; ILE-28; ASN-29; PRO-31; VAL-32 AND THR-33.
    24. Cited for: IMPORTANCE OF TYR-36; ARG-47; LYS-58; LYS-61 AND LYS-72 FOR RECEPTOR BINDING, MUTAGENESIS.
    25. "Identification of the glycosaminoglycan binding site of the CC chemokine, MCP-1: implications for structure and function in vivo."
      Lau E.K., Paavola C.D., Johnson Z., Gaudry J.-P., Geretti E., Borlat F., Kungl A.J., Proudfoot A.E., Handel T.M.
      J. Biol. Chem. 279:22294-22305(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: GAG BINDING SITES ARG-41; LYS-42 AND ARG-47, MUTAGENESIS.
    26. "Fat-specific protein 27 modulates nuclear factor of activated T cells 5 and the cellular response to stress."
      Ueno M., Shen W.J., Patel S., Greenberg A.S., Azhar S., Kraemer F.B.
      J. Lipid Res. 54:734-743(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION BY OSMOTIC STRESS.
    27. "Modeling the three-dimensional structure of the monocyte chemo-attractant and activating protein MCAF/MCP-1 on the basis of the solution structure of interleukin-8."
      Gronenborn A.M., Clore G.M.
      Protein Eng. 4:263-269(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: 3D-STRUCTURE MODELING.
    28. "Heteronuclear (1H, 13C, 15N) NMR assignments and solution structure of the monocyte chemoattractant protein-1 (MCP-1) dimer."
      Handel T.M., Domaille P.J.
      Biochemistry 35:6569-6584(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR.
    29. "The structure of MCP-1 in two crystal forms provides a rare example of variable quaternary interactions."
      Lubkowski J., Bujacz G., Domaille P.J., Handel T.M., Wlodawer A.
      Nat. Struct. Biol. 4:64-69(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS).
    30. "A functional promoter polymorphism in monocyte chemoattractant protein-1 is associated with increased susceptibility to pulmonary tuberculosis."
      Flores-Villanueva P.O., Ruiz-Morales J.A., Song C.-H., Flores L.M., Jo E.-K., Montano M., Barnes P.F., Selman M., Granados J.
      J. Exp. Med. 202:1649-1658(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INVOLVEMENT IN MYCOBACTERIUM TUBERCULOSIS SUSCEPTIBILITY.

    Entry informationi

    Entry nameiCCL2_HUMAN
    AccessioniPrimary (citable) accession number: P13500
    Secondary accession number(s): B2R4V3, Q9UDF3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: January 1, 1990
    Last modified: October 1, 2014
    This is version 182 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 17
      Human chromosome 17: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3