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Protein

Glutamine synthetase

Gene

glnA

Organism
Rhodobacter capsulatus (Rhodopseudomonas capsulata)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent biosynthesis of glutamine from glutamate and ammonia.By similarity

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.By similarity

Cofactori

Mg2+By similarityNote: Binds 2 Mg2+ ions per subunit.By similarity

Enzyme regulationi

The activity of this enzyme could be controlled by adenylation under conditions of abundant glutamine.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi130Magnesium 1By similarity1
Metal bindingi132Magnesium 2By similarity1
Binding sitei208ATPBy similarity1
Metal bindingi213Magnesium 2By similarity1
Metal bindingi220Magnesium 2By similarity1
Binding sitei265L-glutamate; via carbonyl oxygenBy similarity1
Metal bindingi269Magnesium 1; via pros nitrogenBy similarity1
Binding sitei273ATPBy similarity1
Binding sitei321L-glutamateBy similarity1
Binding sitei327L-glutamateBy similarity1
Binding sitei339ATPBy similarity1
Binding sitei339L-glutamateBy similarity1
Binding sitei344ATPBy similarity1
Binding sitei352ATPBy similarity1
Metal bindingi357Magnesium 1By similarity1
Binding sitei359L-glutamateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi271 – 273ATPBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processNitrogen fixation
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetaseBy similarity (EC:6.3.1.2By similarity)
Short name:
GSBy similarity
Alternative name(s):
Glutamate--ammonia ligaseCurated
Glutamine synthetase I betaBy similarity
Short name:
GSI betaBy similarity
Gene namesi
Name:glnABy similarity
OrganismiRhodobacter capsulatus (Rhodopseudomonas capsulata)
Taxonomic identifieri1061 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRhodobacter

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001532551 – ›438Glutamine synthetaseAdd BLAST›438

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei397O-AMP-tyrosineBy similarity1

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Oligomer of 12 subunits arranged in the form of two hexameric ring.By similarity

Protein-protein interaction databases

STRINGi272942.RCAP_rcc01674.

Structurei

3D structure databases

ProteinModelPortaliP13499.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni264 – 265L-glutamate bindingBy similarity2

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Phylogenomic databases

eggNOGiENOG4105C5F. Bacteria.
COG0174. LUCA.

Family and domain databases

Gene3Di3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProiView protein in InterPro
IPR008147. Gln_synt_b-grasp.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR004809. Gln_synth_I.
IPR027302. Gln_synth_N_conserv_site.
PfamiView protein in Pfam
PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
SMARTiView protein in SMART
SM01230. Gln-synt_C. 1 hit.
SUPFAMiSSF54368. SSF54368. 1 hit.
TIGRFAMsiTIGR00653. GlnA. 1 hit.
PROSITEiView protein in PROSITE
PS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.

Sequencei

Sequence statusi: Fragment.

P13499-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAVKKALDL MKAEEVEYVD IRFCDPRGKL QHVTLIADLV DEDFFEEGFM
60 70 80 90 100
FDGSSIAGWK SIDQSDMKLI PDASSVYIDP FYAEKTMCVH CNVVEPDTAE
110 120 130 140 150
AYSRDPRIAL KAEAYLKASG IGDVAYFGPE AEFFIFDDVR YSVTPAKVAY
160 170 180 190 200
QIDAEAAAWN TDAEVEMGNL AHRAGHKGGY FPVNPVDEAQ DLRGEMLSTM
210 220 230 240 250
KRMGMKVDKH HHEVATCQHE LGLIFGGLTE QADNILKYKY VIHNVAHAYG
260 270 280 290 300
KTVTFMPKPM KGDNGSGMHV NMSIWKDGKP LFAGDKYADL SQEALYFIGG
310 320 330 340 350
ILKHAKALNA LTNPGTNSYK RLIPGFEAPV LRAYSARNRS GCVRIPWTES
360 370 380 390 400
PKAKRVEARF PDPSANPYLA FAALLMAGLD GIKNKIDPGP ASDKDLYDLP
410 420 430
PEELAAIPTV CGSLREALTE LEKDHDFLLA GDVFTKDQ
Length:438
Mass (Da):48,391
Last modified:October 1, 1996 - v2
Checksum:i07B81EBF6534991F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-terminal residuei4381

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U25953 Genomic DNA. Translation: AAA87025.1.
M28244 Genomic DNA. Translation: AAA26123.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U25953 Genomic DNA. Translation: AAA87025.1.
M28244 Genomic DNA. Translation: AAA26123.1.

3D structure databases

ProteinModelPortaliP13499.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272942.RCAP_rcc01674.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4105C5F. Bacteria.
COG0174. LUCA.

Family and domain databases

Gene3Di3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProiView protein in InterPro
IPR008147. Gln_synt_b-grasp.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR004809. Gln_synth_I.
IPR027302. Gln_synth_N_conserv_site.
PfamiView protein in Pfam
PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
SMARTiView protein in SMART
SM01230. Gln-synt_C. 1 hit.
SUPFAMiSSF54368. SSF54368. 1 hit.
TIGRFAMsiTIGR00653. GlnA. 1 hit.
PROSITEiView protein in PROSITE
PS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGLN1B_RHOCA
AccessioniPrimary (citable) accession number: P13499
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: October 1, 1996
Last modified: April 12, 2017
This is version 88 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.