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Protein

Dynactin subunit 1

Gene

Gl

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for the cytoplasmic dynein-driven retrograde movement of vesicles and organelles along microtubules. Dynein-dynactin interaction is a key component of the mechanism of axonal transport of vesicles and organelles.

GO - Molecular functioni

  • dynein binding Source: FlyBase
  • dynein complex binding Source: FlyBase
  • microtubule motor activity Source: FlyBase

GO - Biological processi

  • axon extension Source: FlyBase
  • cell fate determination Source: FlyBase
  • cellularization Source: FlyBase
  • centrosome localization Source: FlyBase
  • centrosome separation Source: FlyBase
  • compound eye photoreceptor cell differentiation Source: FlyBase
  • compound eye photoreceptor development Source: FlyBase
  • establishment of epithelial cell apical/basal polarity Source: FlyBase
  • eye photoreceptor cell differentiation Source: FlyBase
  • intracellular protein transport Source: FlyBase
  • kinetochore organization Source: FlyBase
  • melanotic encapsulation of foreign target Source: FlyBase
  • microtubule-based movement Source: FlyBase
  • mitochondrion transport along microtubule Source: FlyBase
  • mitotic nuclear division Source: FlyBase
  • mitotic spindle midzone assembly Source: FlyBase
  • mitotic spindle organization Source: FlyBase
  • mRNA transport Source: FlyBase
  • neurogenesis Source: FlyBase
  • oogenesis Source: FlyBase
  • protein localization to kinetochore Source: FlyBase
  • retrograde axonal transport Source: FlyBase
  • retrograde synaptic vesicle transport Source: FlyBase
  • rhabdomere development Source: FlyBase
  • second mitotic wave involved in compound eye morphogenesis Source: FlyBase
  • spindle assembly Source: FlyBase
Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-DME-6807878. COPI-mediated anterograde transport.
R-DME-6811436. COPI-independent Golgi-to-ER retrograde traffic.

Names & Taxonomyi

Protein namesi
Recommended name:
Dynactin subunit 1
Alternative name(s):
150 kDa dynein-associated polypeptide
Short name:
DAP-150
Short name:
DP-150
Protein glued
Gene namesi
Name:Gl
ORF Names:CG9206
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0001108. Gl.

Subcellular locationi

GO - Cellular componenti

  • aster Source: FlyBase
  • axon cytoplasm Source: GOC
  • centrosome Source: FlyBase
  • cytoplasm Source: FlyBase
  • dynactin complex Source: FlyBase
  • dynein complex Source: UniProtKB-KW
  • kinetochore Source: FlyBase
  • kinetochore microtubule Source: FlyBase
  • microtubule plus-end Source: FlyBase
  • midbody Source: FlyBase
  • spindle microtubule Source: FlyBase
  • spindle pole centrosome Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Dynein, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12651265Dynactin subunit 1PRO_0000083523Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei85 – 851Phosphoserine1 Publication
Modified residuei110 – 1101Phosphoserine1 Publication
Modified residuei114 – 1141Phosphoserine1 Publication
Modified residuei117 – 1171Phosphoserine1 Publication
Modified residuei121 – 1211Phosphoserine1 Publication
Modified residuei183 – 1831Phosphoserine1 Publication
Modified residuei1117 – 11171Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP13496.

PTM databases

iPTMnetiP13496.

Expressioni

Gene expression databases

BgeeiFBgn0001108.
GenevisibleiP13496. DM.

Interactioni

Subunit structurei

Large macromolecular complex of at least 10 components; p150(glued) binds directly to microtubules and to cytoplasmic dynein.

GO - Molecular functioni

  • dynein binding Source: FlyBase
  • dynein complex binding Source: FlyBase

Protein-protein interaction databases

BioGridi64877. 13 interactions.
DIPiDIP-21099N.
IntActiP13496. 6 interactions.
MINTiMINT-342315.
STRINGi7227.FBpp0075498.

Structurei

3D structure databases

ProteinModelPortaliP13496.
SMRiP13496. Positions 6-77.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini27 – 6943CAP-GlyPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili213 – 570358Sequence analysisAdd
BLAST
Coiled coili812 – 83625Sequence analysisAdd
BLAST
Coiled coili967 – 1084118Sequence analysisAdd
BLAST
Coiled coili1128 – 116033Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi105 – 13834Ser-richAdd
BLAST

Sequence similaritiesi

Belongs to the dynactin 150 kDa subunit family.Curated
Contains 1 CAP-Gly domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IQSC. Eukaryota.
ENOG410XPXM. LUCA.
GeneTreeiENSGT00760000119173.
InParanoidiP13496.
KOiK04648.
OMAiPEDSTMQ.
OrthoDBiEOG091G0WO0.
PhylomeDBiP13496.

Family and domain databases

Gene3Di2.30.30.190. 1 hit.
InterProiIPR000938. CAP-Gly_domain.
IPR027663. DCTN1.
IPR022157. Dynactin.
[Graphical view]
PANTHERiPTHR18916:SF40. PTHR18916:SF40. 1 hit.
PfamiPF01302. CAP_GLY. 1 hit.
PF12455. Dynactin. 1 hit.
[Graphical view]
SMARTiSM01052. CAP_GLY. 1 hit.
[Graphical view]
SUPFAMiSSF74924. SSF74924. 1 hit.
PROSITEiPS00845. CAP_GLY_1. 1 hit.
PS50245. CAP_GLY_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P13496-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEKNLKVGA RVELTGKDLL GTVAYVGMTS FAVGKWVGVV LDEPKGKNSG
60 70 80 90 100
SIKGQQYFQC DENCGMFVRP TQLRLLEAAP GSRRSIEDVS GATPTAAQPT
110 120 130 140 150
KARLSSSRTS LSSSRQSLLG SRTQLTTSLS ERTASSSSIG PRKSLAPQNS
160 170 180 190 200
KDKESPSTSL AEGAPAASGG NGAASHASSK RASFVETGFL EILKPQFTPS
210 220 230 240 250
QPLRSPSFTM PSNSGAEDKV ALLEAQKTSA ELQAQLADLT EKLETLKQRR
260 270 280 290 300
NEDKERLREF DKMKIQFEQL QEFRTKIMGA QASLQKELLR AKQEAKDAIE
310 320 330 340 350
AKEQHAQEMA DLADNVEMIT LDKEMAEEKA DTLQLELESS KERIEELEVD
360 370 380 390 400
LELLRSEMQN KAESAIGNIS GGGDSPGLST YEFKQLEQQN IRLKETLVRL
410 420 430 440 450
RDLSAHDKHD IQKLSKELEM KRSEVTELER TKEKLSAKID ELEAIVADLQ
460 470 480 490 500
EQVDAALGAE EMVEQLAEKK MELEDKVKLL EEEIAQLEAL EEVHEQLVES
510 520 530 540 550
NHELELDLRE ELDLANGAKK EVLRERDAAI ETIYDRDQTI VKFRELVQKL
560 570 580 590 600
NDQLTELRDR NSSNEKESLQ DPSLKMVTET IDYKQMFAES KAYTRAIDVQ
610 620 630 640 650
LRQIELSQAN EHVQMLTAFM PESFMSRGGD HDSILVILLI SRIVFKCDIV
660 670 680 690 700
VSQTRERFPP VDAITREAVT QGHAVQQYAF KCRLLHYVHS LQCALHQILY
710 720 730 740 750
GLNSCQPDTL LRAGSSLPEM VAQEKIVDGI IELLKSNQLD ENSTTDNIEK
760 770 780 790 800
CVAFFNAMNS VLLAGEQLLN EIQMIRDCVA SLGAACESIL SDTAIAKVII
810 820 830 840 850
QEAGATSDSV LLIQFLNENM ESVRQQVKLI KRRLPSDQHV IKSGLSQHKV
860 870 880 890 900
EAMRGLAQNI SRIMSAMHQA TKQSLAAIVS TIESDNAAEH TLPQEKYWAL
910 920 930 940 950
LTASCERIYE QDDRGPTQNF KTLLAQANSD LQLIAQHLLD KEYDIISAAN
960 970 980 990 1000
NASNQQKSGA HSTPITQRAQ LIKKQLEQKN VLAATLENRE ADVKQLKVAA
1010 1020 1030 1040 1050
KMKQNELSEM QIRKDLAEKK LSVLQNEYEH AVDKWKQKYE ETSLQLQLKE
1060 1070 1080 1090 1100
KEFEETMDHL QSDIDALESE KSDLRDKLKL NSTTGKVQPG SESHSPHNIS
1110 1120 1130 1140 1150
LSGNTSTAPG ISNVSYSAPA GTAPVVAEEV ELLKNAFNQE RNQRLRLQAQ
1160 1170 1180 1190 1200
DMRAKLSQFE PLHVPQPQDQ RITALESELT RMKHAWVLSL LQVRSQDSVN
1210 1220 1230 1240 1250
SGTRIDAVAL QRRNQPVPLK GEISSKASQL ASDILTEYLQ RKPHRATHGQ
1260
FASFPTVDVK RVLQI
Length:1,265
Mass (Da):141,218
Last modified:December 1, 2000 - v2
Checksum:i2038A200282B2755
GO

Sequence cautioni

The sequence J02932 differs from that shown. Reason: Frameshift at positions 32, 174, 220, 648, 672 and 1208. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti708 – 7081D → A in J02932 (PubMed:2819881).Curated
Sequence conflicti865 – 8651S → P in AAM48406 (PubMed:12537569).Curated
Sequence conflicti875 – 8751L → V in J02932 (PubMed:2819881).Curated
Sequence conflicti888 – 8881A → R in J02932 (PubMed:2819881).Curated
Sequence conflicti1043 – 10431S → C in J02932 (PubMed:2819881).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02932 mRNA. No translation available.
AE014296 Genomic DNA. Translation: AAF49788.1.
AY118377 mRNA. Translation: AAM48406.1.
BT044559 mRNA. Translation: ACI15754.1.
PIRiA28313.
RefSeqiNP_524061.1. NM_079337.3.
UniGeneiDm.12980.

Genome annotation databases

EnsemblMetazoaiFBtr0075756; FBpp0075498; FBgn0001108.
GeneIDi39536.
KEGGidme:Dmel_CG9206.
UCSCiCG9206-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02932 mRNA. No translation available.
AE014296 Genomic DNA. Translation: AAF49788.1.
AY118377 mRNA. Translation: AAM48406.1.
BT044559 mRNA. Translation: ACI15754.1.
PIRiA28313.
RefSeqiNP_524061.1. NM_079337.3.
UniGeneiDm.12980.

3D structure databases

ProteinModelPortaliP13496.
SMRiP13496. Positions 6-77.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi64877. 13 interactions.
DIPiDIP-21099N.
IntActiP13496. 6 interactions.
MINTiMINT-342315.
STRINGi7227.FBpp0075498.

PTM databases

iPTMnetiP13496.

Proteomic databases

PaxDbiP13496.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0075756; FBpp0075498; FBgn0001108.
GeneIDi39536.
KEGGidme:Dmel_CG9206.
UCSCiCG9206-RA. d. melanogaster.

Organism-specific databases

CTDi39536.
FlyBaseiFBgn0001108. Gl.

Phylogenomic databases

eggNOGiENOG410IQSC. Eukaryota.
ENOG410XPXM. LUCA.
GeneTreeiENSGT00760000119173.
InParanoidiP13496.
KOiK04648.
OMAiPEDSTMQ.
OrthoDBiEOG091G0WO0.
PhylomeDBiP13496.

Enzyme and pathway databases

ReactomeiR-DME-6807878. COPI-mediated anterograde transport.
R-DME-6811436. COPI-independent Golgi-to-ER retrograde traffic.

Miscellaneous databases

GenomeRNAii39536.
PROiP13496.

Gene expression databases

BgeeiFBgn0001108.
GenevisibleiP13496. DM.

Family and domain databases

Gene3Di2.30.30.190. 1 hit.
InterProiIPR000938. CAP-Gly_domain.
IPR027663. DCTN1.
IPR022157. Dynactin.
[Graphical view]
PANTHERiPTHR18916:SF40. PTHR18916:SF40. 1 hit.
PfamiPF01302. CAP_GLY. 1 hit.
PF12455. Dynactin. 1 hit.
[Graphical view]
SMARTiSM01052. CAP_GLY. 1 hit.
[Graphical view]
SUPFAMiSSF74924. SSF74924. 1 hit.
PROSITEiPS00845. CAP_GLY_1. 1 hit.
PS50245. CAP_GLY_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDCTN1_DROME
AccessioniPrimary (citable) accession number: P13496
Secondary accession number(s): B5X0J0, Q8MT52, Q9VUA1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: December 1, 2000
Last modified: September 7, 2016
This is version 162 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.