Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P13491

- LDHA_RABIT

UniProt

P13491 - LDHA_RABIT

Protein

L-lactate dehydrogenase A chain

Gene

LDHA

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalytic activityi

    (S)-lactate + NAD+ = pyruvate + NADH.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei99 – 991NAD
    Binding sitei106 – 1061Substrate
    Binding sitei138 – 1381NAD or substrate
    Binding sitei169 – 1691Substrate
    Active sitei193 – 1931Proton acceptor1 Publication
    Binding sitei248 – 2481Substrate

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi29 – 5729NADAdd
    BLAST

    GO - Molecular functioni

    1. L-lactate dehydrogenase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cellular carbohydrate metabolic process Source: InterPro
    2. glycolytic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    SABIO-RKP13491.
    UniPathwayiUPA00554; UER00611.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    L-lactate dehydrogenase A chain (EC:1.1.1.27)
    Short name:
    LDH-A
    Alternative name(s):
    LDH muscle subunit
    Short name:
    LDH-M
    Gene namesi
    Name:LDHA
    OrganismiOryctolagus cuniculus (Rabbit)
    Taxonomic identifieri9986 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
    ProteomesiUP000001811: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 332331L-lactate dehydrogenase A chainPRO_0000168418Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity
    Modified residuei5 – 51N6-acetyllysine; alternateBy similarity
    Modified residuei5 – 51N6-succinyllysine; alternateBy similarity
    Modified residuei14 – 141N6-acetyllysineBy similarity
    Modified residuei57 – 571N6-acetyllysineBy similarity
    Modified residuei81 – 811N6-acetyllysineBy similarity
    Modified residuei118 – 1181N6-acetyllysine; alternateBy similarity
    Modified residuei118 – 1181N6-succinyllysine; alternateBy similarity
    Modified residuei126 – 1261N6-acetyllysineBy similarity
    Modified residuei224 – 2241N6-acetyllysineBy similarity
    Modified residuei232 – 2321N6-acetyllysineBy similarity
    Modified residuei239 – 2391PhosphotyrosineBy similarity
    Modified residuei243 – 2431N6-acetyllysineBy similarity
    Modified residuei318 – 3181N6-acetyllysine; alternateBy similarity
    Modified residuei318 – 3181N6-succinyllysine; alternateBy similarity

    Post-translational modificationi

    ISGylated.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein, Ubl conjugation

    Proteomic databases

    PRIDEiP13491.

    Interactioni

    Subunit structurei

    Homotetramer.1 Publication

    Protein-protein interaction databases

    IntActiP13491. 1 interaction.
    STRINGi9986.ENSOCUP00000021667.

    Structurei

    Secondary structure

    1
    332
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi4 – 85
    Beta strandi9 – 113
    Beta strandi20 – 267
    Helixi30 – 4112
    Beta strandi46 – 516
    Helixi55 – 6612
    Helixi67 – 715
    Beta strandi76 – 794
    Helixi83 – 864
    Beta strandi90 – 945
    Helixi109 – 12719
    Beta strandi132 – 1354
    Beta strandi137 – 1393
    Helixi140 – 15112
    Helixi155 – 1573
    Beta strandi158 – 1603
    Helixi164 – 17815
    Helixi182 – 1843
    Beta strandi189 – 1946
    Helixi201 – 2033
    Helixi211 – 2144
    Turni216 – 2194
    Helixi228 – 24518
    Helixi250 – 26415
    Beta strandi269 – 2768
    Beta strandi288 – 2969
    Beta strandi299 – 3046
    Helixi310 – 32718

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3H3FX-ray2.38A/B/C/D/E/F/G/H2-332[»]
    4I8XX-ray2.23A/B/C/D/E/F/G/H2-332[»]
    4I9HX-ray2.17A/B/C/D/E/F/G/H2-332[»]
    4I9NX-ray2.35A/B/C/D/E/F/G/H2-332[»]
    4I9UX-ray2.50A/B/C/D/E/F/G/H2-332[»]
    ProteinModelPortaliP13491.
    SMRiP13491. Positions 2-332.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP13491.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the LDH/MDH superfamily. LDH family.Curated

    Phylogenomic databases

    eggNOGiCOG0039.
    HOGENOMiHOG000213793.
    HOVERGENiHBG000462.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    HAMAPiMF_00488. Lactate_dehydrog.
    InterProiIPR001557. L-lactate/malate_DH.
    IPR011304. L-lactate_DH.
    IPR018177. L-lactate_DH_AS.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR11540. PTHR11540. 1 hit.
    PfamiPF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
    PRINTSiPR00086. LLDHDRGNASE.
    SUPFAMiSSF56327. SSF56327. 1 hit.
    TIGRFAMsiTIGR01771. L-LDH-NAD. 1 hit.
    PROSITEiPS00064. L_LDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P13491-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAALKDQLIH NLLKEEHVPQ NKITVVGVGA VGMACAISIL MKDLADELAL    50
    VDVMEDKLKG EMMDLQHGSL FLRTPKIVSG KDYSVTANSK LVIITAGARQ 100
    QEGESRLNLV QRNVNIFKFI IPNVVKYSPH CKLLVVSNPV DILTYVAWKI 150
    SGFPKNRVIG SGCNLDSARF RYLMGERLGV HALSCHGWIL GEHGDSSVPV 200
    WSGMNVAGVS LKTLHPELGT DADKEQWKQV HKQVVDSAYE VIKLKGYTTW 250
    AIGLSVADLA ESIMKNLRRV HPISTMLKGL YGIKEDVFLS VPCVLGQNGI 300
    SDVVKVTLTS EEEAHLKKSA DTLWGIQKEL QF 332
    Length:332
    Mass (Da):36,565
    Last modified:January 23, 2007 - v3
    Checksum:i84E86C2E7D018EF7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M22585 mRNA. Translation: AAA31382.1.
    PIRiA32957.
    RefSeqiNP_001075746.1. NM_001082277.1.
    UniGeneiOcu.1108.

    Genome annotation databases

    GeneIDi100009107.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M22585 mRNA. Translation: AAA31382.1 .
    PIRi A32957.
    RefSeqi NP_001075746.1. NM_001082277.1.
    UniGenei Ocu.1108.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3H3F X-ray 2.38 A/B/C/D/E/F/G/H 2-332 [» ]
    4I8X X-ray 2.23 A/B/C/D/E/F/G/H 2-332 [» ]
    4I9H X-ray 2.17 A/B/C/D/E/F/G/H 2-332 [» ]
    4I9N X-ray 2.35 A/B/C/D/E/F/G/H 2-332 [» ]
    4I9U X-ray 2.50 A/B/C/D/E/F/G/H 2-332 [» ]
    ProteinModelPortali P13491.
    SMRi P13491. Positions 2-332.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P13491. 1 interaction.
    STRINGi 9986.ENSOCUP00000021667.

    Proteomic databases

    PRIDEi P13491.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 100009107.

    Organism-specific databases

    CTDi 3939.

    Phylogenomic databases

    eggNOGi COG0039.
    HOGENOMi HOG000213793.
    HOVERGENi HBG000462.

    Enzyme and pathway databases

    UniPathwayi UPA00554 ; UER00611 .
    SABIO-RK P13491.

    Miscellaneous databases

    EvolutionaryTracei P13491.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    HAMAPi MF_00488. Lactate_dehydrog.
    InterProi IPR001557. L-lactate/malate_DH.
    IPR011304. L-lactate_DH.
    IPR018177. L-lactate_DH_AS.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR11540. PTHR11540. 1 hit.
    Pfami PF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000102. Lac_mal_DH. 1 hit.
    PRINTSi PR00086. LLDHDRGNASE.
    SUPFAMi SSF56327. SSF56327. 1 hit.
    TIGRFAMsi TIGR01771. L-LDH-NAD. 1 hit.
    PROSITEi PS00064. L_LDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of rabbit lactate dehydrogenase-M and lactate dehydrogenase-H cDNAs. Control of lactate dehydrogenase expression in rabbit muscle."
      Sass C., Briand M., Benslimane S., Renaud M., Briand Y.
      J. Biol. Chem. 264:4076-4081(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Modeling of isotope effects on binding oxamate to lactic dehydrogenase."
      Swiderek K., Panczakiewicz A., Bujacz A., Bujacz G., Paneth P.
      J. Phys. Chem. B 113:12782-12789(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.38 ANGSTROMS) OF 2-332 IN COMPLEX WITH NADH AMD INHIBITOR, SUBUNIT, ACTIVE SITE, SUBSTRATE-BINDING SITES, NAD-BINDING SITES.

    Entry informationi

    Entry nameiLDHA_RABIT
    AccessioniPrimary (citable) accession number: P13491
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 117 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3