Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P13487

- KAX11_LEIQH

UniProt

P13487 - KAX11_LEIQH

Protein

Potassium channel toxin alpha-KTx 1.1

Gene
N/A
Organism
Leiurus quinquestriatus hebraeus (Yellow scorpion)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Potent selective inhibitor of high conductance (maxi-K), different medium and small conductance calcium-activated potassium channels (KCa/KCNM), as well as a voltage-dependent potassium channel (Kv1.3/KCNA3). It appears to block channel activity by a simple bimolecular inhibition process.1 Publication
    Has a pH-specific antimicrobial activity against bacteria (B.subtilis, E.coli and S.aureus) and the fungus C.albicans.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei49 – 491Basic residue of the functional dyadBy similarity
    Sitei58 – 581Aromatic residue of the functional dyadBy similarity

    GO - Molecular functioni

    1. ion channel inhibitor activity Source: InterPro

    GO - Biological processi

    1. defense response to bacterium Source: UniProtKB-KW
    2. defense response to fungus Source: UniProtKB-KW
    3. killing of cells of other organism Source: UniProtKB-KW
    4. pathogenesis Source: InterPro

    Keywords - Molecular functioni

    Antibiotic, Antimicrobial, Calcium-activated potassium channel impairing toxin, Fungicide, Ion channel impairing toxin, Neurotoxin, Potassium channel impairing toxin, Toxin, Voltage-gated potassium channel impairing toxin

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Potassium channel toxin alpha-KTx 1.1
    Alternative name(s):
    ChTX-Lq1
    ChTx-a
    Charybdotoxin
    Short name:
    ChTX
    OrganismiLeiurus quinquestriatus hebraeus (Yellow scorpion)
    Taxonomic identifieri6884 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaScorpionesButhidaButhoideaButhidaeLeiurus

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 22223 PublicationsAdd
    BLAST
    Chaini23 – 5937Potassium channel toxin alpha-KTx 1.1PRO_0000035307Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei23 – 231Pyrrolidone carboxylic acid
    Disulfide bondi29 ↔ 50
    Disulfide bondi35 ↔ 55
    Disulfide bondi39 ↔ 57

    Keywords - PTMi

    Disulfide bond, Pyrrolidone carboxylic acid

    Expressioni

    Tissue specificityi

    Expressed by the venom gland.

    Structurei

    Secondary structure

    1
    59
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi32 – 343
    Helixi36 – 427
    Beta strandi48 – 503
    Beta strandi52 – 554

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1BAHNMR-A24-59[»]
    1CMRNMR-A29-59[»]
    2A9HNMR-E24-59[»]
    2CRDNMR-A24-59[»]
    4JTAX-ray2.50Y23-59[»]
    4JTCX-ray2.56Y23-59[»]
    4JTDX-ray2.54Y23-59[»]
    ProteinModelPortaliP13487.
    SMRiP13487. Positions 23-59.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP13487.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni48 – 558Interaction with Ca(2+)-activated K(+) channelsSequence Analysis

    Domaini

    Has the structural arrangement of an alpha-helix connected to a beta-sheet by disulfide bonds (CSalpha/beta).

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di3.30.30.10. 1 hit.
    InterProiIPR003614. Scorpion_toxin-like.
    IPR001947. Scorpion_toxinS_K_inh.
    [Graphical view]
    PfamiPF00451. Toxin_2. 1 hit.
    [Graphical view]
    PRINTSiPR00286. CHARYBDTOXIN.
    ProDomiPD003586. Scorpion_toxinS. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SUPFAMiSSF57095. SSF57095. 1 hit.
    PROSITEiPS01138. SCORP_SHORT_TOXIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P13487-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKILSVLLLA LIICSIVGWS EAQFTNVSCT TSKECWSVCQ RLHNTSRGKC   50
    MNKKCRCYS 59
    Length:59
    Mass (Da):6,674
    Last modified:November 21, 2003 - v4
    Checksum:i379227EBBCB21947
    GO

    Sequence databases

    PIRiA28202. A60963.

    Cross-referencesi

    Sequence databases

    PIRi A28202. A60963.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1BAH NMR - A 24-59 [» ]
    1CMR NMR - A 29-59 [» ]
    2A9H NMR - E 24-59 [» ]
    2CRD NMR - A 24-59 [» ]
    4JTA X-ray 2.50 Y 23-59 [» ]
    4JTC X-ray 2.56 Y 23-59 [» ]
    4JTD X-ray 2.54 Y 23-59 [» ]
    ProteinModelPortali P13487.
    SMRi P13487. Positions 23-59.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P13487.

    Family and domain databases

    Gene3Di 3.30.30.10. 1 hit.
    InterProi IPR003614. Scorpion_toxin-like.
    IPR001947. Scorpion_toxinS_K_inh.
    [Graphical view ]
    Pfami PF00451. Toxin_2. 1 hit.
    [Graphical view ]
    PRINTSi PR00286. CHARYBDTOXIN.
    ProDomi PD003586. Scorpion_toxinS. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SUPFAMi SSF57095. SSF57095. 1 hit.
    PROSITEi PS01138. SCORP_SHORT_TOXIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Dynamic diversification from a putative common ancestor of scorpion toxins affecting sodium, potassium, and chloride channels."
      Froy O., Sagiv T., Poreh M., Urbach D., Zilberberg N., Gurevitz M.
      J. Mol. Evol. 48:187-196(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Tissue: Single abdominal segment.
    2. "Purification, sequence, and model structure of charybdotoxin, a potent selective inhibitor of calcium-activated potassium channels."
      Gimenez-Gallego G., Navia M.A., Reuben J.P., Katz G.M., Kaczorowski G.J., Garcia M.L.
      Proc. Natl. Acad. Sci. U.S.A. 85:3329-3333(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 23-59.
    3. "Charybdotoxin is a new member of the K+ channel toxin family that includes dendrotoxin I and mast cell degranulating peptide."
      Schweitz H., Bidard J.-N., Maes P., Lazdunski M.
      Biochemistry 28:9708-9714(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 23-59.
    4. "Analysis of the blocking activity of charybdotoxin homologs and iodinated derivatives against Ca2+-activated K+ channels."
      Lucchesi K., Ravindran A., Young H., Moczydlowski E.
      J. Membr. Biol. 109:269-281(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 23-59.
      Tissue: Venom.
    5. Cited for: SYNTHESIS OF 23-59.
    6. "Synthesis and structural characterization of charybdotoxin, a potent peptidyl inhibitor of the high conductance Ca2(+)-activated K+ channel."
      Sugg E.E., Garcia M.L., Reuben J.P., Patchett A.A., Kaczorowski G.J.
      J. Biol. Chem. 265:18745-18748(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: SYNTHESIS OF 23-59, DISULFIDE BONDS.
    7. "Moving pieces in a taxonomic puzzle: venom 2D-LC/MS and data clustering analyses to infer phylogenetic relationships in some scorpions from the Buthidae family (Scorpiones)."
      Nascimento D.G., Rates B., Santos D.M., Verano-Braga T., Barbosa-Silva A., Dutra A.A.A., Biondi I., Martin-Eauclaire M.-F., De Lima M.E., Pimenta A.M.C.
      Toxicon 47:628-639(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
    8. "Multidimensional signatures in antimicrobial peptides."
      Yount N.Y., Yeaman M.R.
      Proc. Natl. Acad. Sci. U.S.A. 101:7363-7368(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    9. "Molecular structure of charybdotoxin, a pore-directed inhibitor of potassium ion channels."
      Massefski W. Jr., Redfield A.G., Hare D.R., Miller C.
      Science 249:521-524(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 23-59, DISULFIDE BONDS.
    10. "Three-dimensional structure of natural charybdotoxin in aqueous solution by 1H-NMR. Charybdotoxin possesses a structural motif found in other scorpion toxins."
      Bontems F., Roumestand C., Boyot P., Gilquin B., Doljansky Y.
      Eur. J. Biochem. 196:19-28(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 23-59, DISULFIDE BONDS.
    11. "Refined structure of charybdotoxin: common motifs in scorpion toxins and insect defensins."
      Bontems F., Roumestand C., Gilquin B., Menez A., Toma F.
      Science 254:1521-1523(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 23-59, DISULFIDE BONDS.
    12. "Analysis of side-chain organization on a refined model of charybdotoxin: structural and functional implications."
      Bontems F., Gilquin B., Roumestand C., Menez A., Toma F.
      Biochemistry 31:7756-7764(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 23-59, DISULFIDE BONDS.
    13. "Progress in multidimensional NMR investigations of peptide and protein 3-D structures in solution. From structure to functional aspects."
      Bonmatin J.-M., Genest M., Petit M.-C., Gincel E., Simorre J.-P., Cornet B., Gallet X., Caille A., Labbe H., Vovelle F., Ptak M.
      Biochimie 74:825-836(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 23-59, DISULFIDE BONDS.
    14. "NMR solution structure of a two-disulfide derivative of charybdotoxin: structural evidence for conservation of scorpion toxin alpha/beta motif and its hydrophobic side chain packing."
      Song J., Gilquin B., Jamin N., Drakopoulou E., Guenneugues M., Dauplais M., Vita C., Menez A.
      Biochemistry 36:3760-3766(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 23-59, DISULFIDE BONDS.

    Entry informationi

    Entry nameiKAX11_LEIQH
    AccessioniPrimary (citable) accession number: P13487
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: November 21, 2003
    Last modified: October 1, 2014
    This is version 116 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programAnimal Toxin Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Scorpion potassium channel toxins
      Nomenclature of scorpion potassium channel toxins and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3