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Protein

Teichoic acid poly(glycerol phosphate) polymerase

Gene

tagF

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Responsible for the polymerization of the main chain of the major teichoic acid by sequential transfer of glycerol phosphate units from CDP-glycerol to the disaccharide linkage unit. Synthesizes polymers of approximately 35 glycerol phosphate units in length.2 Publications

Catalytic activityi

CDP-glycerol + (glycerophosphate)(n) = CMP + (glycerophosphate)(n+1).4 Publications

Kineticsi

kcat for CDP-glycerol is 14 min(-1) (PubMed:16141206). kcat for lipid III analog is 26 sec(-1) (PubMed:18465758).2 Publications
  1. KM=340 µM for CDP-glycerol1 Publication
  2. KM=230 µM for CDP-glycerol1 Publication
  3. KM=152 µM for CDP-glycerol1 Publication
  4. KM=2.6 µM for lipid III analog1 Publication

    pH dependencei

    Optimum pH is 8.1 Publication

    Pathwayi: poly(glycerol phosphate) teichoic acid biosynthesis

    This protein is involved in the pathway poly(glycerol phosphate) teichoic acid biosynthesis, which is part of Cell wall biogenesis.
    View all proteins of this organism that are known to be involved in the pathway poly(glycerol phosphate) teichoic acid biosynthesis and in Cell wall biogenesis.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei540CDP-glycerolBy similarity1
    Binding sitei657CDP-glycerolBy similarity1

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionTransferase
    Biological processCell wall biogenesis/degradation, Teichoic acid biosynthesis

    Enzyme and pathway databases

    BioCyciBSUB:BSU35720-MONOMER.
    MetaCyc:MONOMER-8821.
    SABIO-RKiP13485.
    UniPathwayiUPA00827.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Teichoic acid poly(glycerol phosphate) polymeraseCurated (EC:2.7.8.124 Publications)
    Alternative name(s):
    CDP-glycerol:poly(glycerophosphate) glycerophosphotransferase
    CGPTase
    Major teichoic acid biosynthesis protein F
    Poly(glycerol phosphate) polymerase1 Publication
    Tag polymerase
    Gene namesi
    Name:tagF
    Synonyms:rodC, tag3
    Ordered Locus Names:BSU35720
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    Proteomesi
    • UP000001570 Componenti: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi474H → A: No activity. 1 Publication1
    Mutagenesisi604E → A: No effect on catalytic efficiency. 1 Publication1
    Mutagenesisi612H → A: No activity. 1 Publication1
    Mutagenesisi630D → A: 3-fold reduction in catalytic efficiency. 1 Publication1
    Mutagenesisi639E → A: 4-fold reduction in catalytic efficiency. 1 Publication1
    Mutagenesisi645D → A: 3-fold reduction in catalytic efficiency. 1 Publication1
    Mutagenesisi650D → A: Did not get expressed in heterologous host. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000724221 – 746Teichoic acid poly(glycerol phosphate) polymeraseAdd BLAST746

    Proteomic databases

    PaxDbiP13485.
    PRIDEiP13485.

    Expressioni

    Inductioni

    Positively regulated by WalR. Mainly expressed during exponential growth and rapidly shut off as cells enter the stationary phase.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    tagBP276213EBI-6401722,EBI-6401730

    Protein-protein interaction databases

    IntActiP13485. 6 interactors.
    STRINGi224308.Bsubs1_010100019326.

    Structurei

    3D structure databases

    ProteinModelPortaliP13485.
    SMRiP13485.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni473 – 477CDP-glycerol bindingBy similarity5
    Regioni573 – 574CDP-glycerol bindingBy similarity2
    Regioni610 – 612CDP-glycerol bindingBy similarity3
    Regioni652 – 653CDP-glycerol bindingBy similarity2

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiENOG4107SJB. Bacteria.
    COG1887. LUCA.
    HOGENOMiHOG000009147.
    InParanoidiP13485.
    KOiK09809.
    OMAiKFCYLES.
    PhylomeDBiP13485.

    Family and domain databases

    InterProiView protein in InterPro
    IPR007554. Glycerophosphate_synth.
    PfamiView protein in Pfam
    PF04464. Glyphos_transf. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P13485-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSLVVDTNKR KQKGKSFYTE EQKKVMIENT VIKCILKSLK NNLGSLELLI
    60 70 80 90 100
    SIDSEHQFLE DYQLFLKLKE RRSGTESEFP LQNTGSLEYK TEINAHVLPM
    110 120 130 140 150
    PVEMGQTYDF YVEFRKKYED AEQEPLLKRL SAEVNSIERA FHVDQTTELL
    160 170 180 190 200
    ILPYTTDKGN FSIKVKREAK IIRFDQIEIS SEEISITGYA GYLSSENQYR
    210 220 230 240 250
    IKNLNLILKK GGETPIEEKF PIKLERKTHG LENMRADGFV PELYDFEVKV
    260 270 280 290 300
    PLKEIPFSNE KRYVYRLFME YICNDDEGTD IQFNSTALVL GDRKNKLKGL
    310 320 330 340 350
    VSIIKTNNAP VRYEVFKKKK KQTLGIRVND YSLKTRMKYF IKGKKKRLVS
    360 370 380 390 400
    KIKKITKMRN KLITKTYKSL FMMASRMPVK RKTVIFESFN GKQYSCNPRA
    410 420 430 440 450
    IYEYMRENHP EYKMYWSVNK QYSAPFDEKG IPYINRLSLK WLFAMARAEY
    460 470 480 490 500
    WVVNSRLPLW IPKPSHTTYL QTWHGTPLKR LAMDMEEVHM PGTNTKKYKR
    510 520 530 540 550
    NFIKEASNWD YLISPNGYST EIFTRAFQFN KTMIESGYPR NDFLHNDNNE
    560 570 580 590 600
    ETISLIKSRL NIPRDKKVIL YAPTWRDDQF YAKGRYKFDL DLDLHQLRQE
    610 620 630 640 650
    LGNEYIVILR MHYLVAENFD LGPFEGFAYD FSAYEDIREL YMVSDLLITD
    660 670 680 690 700
    YSSVFFDFAN LKRPMLFFVP DIETYRDKLR GFYFDFEKEA PGPLVKTTEE
    710 720 730 740
    TIEAIKQISS PDYKLPVSFG PFYDKFCYLE SGRSSEKVVN TVFKAE
    Length:746
    Mass (Da):88,063
    Last modified:January 1, 1990 - v1
    Checksum:i9FEB94D83332B980
    GO

    Natural variant

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Natural varianti644S → F in mutant rodC1; temperature-sensitive. 1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X15200 Genomic DNA. Translation: CAA33271.1.
    AL009126 Genomic DNA. Translation: CAB15589.1.
    PIRiS06049.
    RefSeqiNP_391453.1. NC_000964.3.
    WP_003243463.1. NZ_JNCM01000034.1.

    Genome annotation databases

    EnsemblBacteriaiCAB15589; CAB15589; BSU35720.
    GeneIDi936803.
    KEGGibsu:BSU35720.
    PATRICi18979160. VBIBacSub10457_3742.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X15200 Genomic DNA. Translation: CAA33271.1.
    AL009126 Genomic DNA. Translation: CAB15589.1.
    PIRiS06049.
    RefSeqiNP_391453.1. NC_000964.3.
    WP_003243463.1. NZ_JNCM01000034.1.

    3D structure databases

    ProteinModelPortaliP13485.
    SMRiP13485.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    IntActiP13485. 6 interactors.
    STRINGi224308.Bsubs1_010100019326.

    Proteomic databases

    PaxDbiP13485.
    PRIDEiP13485.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiCAB15589; CAB15589; BSU35720.
    GeneIDi936803.
    KEGGibsu:BSU35720.
    PATRICi18979160. VBIBacSub10457_3742.

    Phylogenomic databases

    eggNOGiENOG4107SJB. Bacteria.
    COG1887. LUCA.
    HOGENOMiHOG000009147.
    InParanoidiP13485.
    KOiK09809.
    OMAiKFCYLES.
    PhylomeDBiP13485.

    Enzyme and pathway databases

    UniPathwayiUPA00827.
    BioCyciBSUB:BSU35720-MONOMER.
    MetaCyc:MONOMER-8821.
    SABIO-RKiP13485.

    Family and domain databases

    InterProiView protein in InterPro
    IPR007554. Glycerophosphate_synth.
    PfamiView protein in Pfam
    PF04464. Glyphos_transf. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiTAGF_BACSU
    AccessioniPrimary (citable) accession number: P13485
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: January 1, 1990
    Last modified: March 15, 2017
    This is version 124 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.