P13483 (DATI_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Oligo(A)/oligo(T)-binding protein

Alternative name(s):
Datin

Gene names

Name:	DAT1
Synonyms:	DAT
Ordered Locus Names:	YML113W
ORF Names:	YM8339.06

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	248 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	DNA-binding protein that recognizes oligo(A).oligo(T) tracts (A.T DNA). Can bind to any 11 bp sequence in which 10 bases conform to an uninterrupted oligo(A).oligo(T) tract.
Subunit structure	Binds as a dimer or higher oligomer.
Miscellaneous	Present with 4700 molecules/cell in log phase SD medium. Ref.6

Ontologies

Keywords
Domain	Repeat
Ligand	DNA-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	negative regulation of transcription from RNA polymerase II promoter Inferred from mutant phenotype. Source: SGD
Cellular component	nucleus Inferred from direct assay. Source: SGD
Molecular function	AT DNA binding Inferred from direct assay Ref.1. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 248	248	Oligo(A)/oligo(T)-binding protein		PRO_0000079787
Regions
	Repeat	8 – 12	5	1
	Repeat	14 – 18	5	2
	Repeat	26 – 30	5	3
	DNA binding	1 – 36	36
	Region	8 – 30	23	3 X 5 AA repeats of G-R-K-P-G
	Compositional bias	111 – 127	17	Poly-Gln
	Compositional bias	155 – 188	34	Asn-rich

Sequences

	Sequence		Length	Mass (Da)
	P13483 [UniParc]. Last modified January 1, 1990. Version 1. Checksum: D0E1E31C0C42A443	FASTA	248	27,067
	10 20 30 40 50 60 MAKTLAQGRK PGSGRKPGKG KTLREGRKPG SGRRRRQDTG GKETDGSQQD QESRLISSRD 70 80 90 100 110 120 MEAVDALREL THSPSSHSAH NSSAAPPPHA AAASTSLPPS LDYTHQSFMD QQQQQQQQQQ 130 140 150 160 170 180 QQLLQQQRVD VVPPKPFITH KILLSSTGNS GGHVNSNYNA DHSINHNSNH NLNSNVNVNM 190 200 210 220 230 240 NFTINGSNQD PSSSFLMGPY NYLQRPFIVK PYLDLSTSTA ASNQPRTQPS PAAHITKNSD STEKNATI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A DNA binding protein that recognizes oligo(dA).oligo(dT) tracts." Winter E., Varshavsky A. EMBO J. 8:1867-1877(1989) [PubMed: 2670564] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"DNA binding properties of the Saccharomyces cerevisiae DAT1 gene product." Reardon B.J., Gordon D., Ballard M.J., Winter E. Nucleic Acids Res. 23:4900-4906(1995) [PubMed: 8532535] [Abstract] Cited for: CHARACTERIZATION.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X15478 Genomic DNA. Translation: CAA33505.1. Z49210 Genomic DNA. Translation: CAA89105.1. AY557765 Genomic DNA. Translation: AAS56091.1. BK006946 Genomic DNA. Translation: DAA09785.1.
PIR	S22853.
RefSeq	NP_013594.1. NM_001182475.1.
3D structure databases
ProteinModelPortal	P13483.
ModBase	Search...
Protein-protein interaction databases
STRING	P13483.
Proteomic databases
PeptideAtlas	P13483.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YML113W; YML113W; YML113W.
GeneID	854927.
KEGG	sce:YML113W.
NMPDR	fig|4932.3.peg.4630.
Organism-specific databases
CYGD	YML113w.
SGD	S000004581. DAT1.
Gene expression databases
ArrayExpress	P13483.
Genevestigator	P13483.
GermOnline	YML113W. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other
NextBio	977950.

Entry information

Entry name

DATI_YEAST

Accession

Primary (citable) accession number: P13483
Secondary accession number(s): D6W0H1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 1, 1990
Last modified:	December 14, 2011
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program