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Protein

Periplasmic trehalase

Gene

treA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Provides the cells with the ability to utilize trehalose at high osmolarity by splitting it into glucose molecules that can subsequently be taken up by the phosphotransferase-mediated uptake system.

Catalytic activityi

Alpha,alpha-trehalose + H2O = beta-D-glucose + alpha-D-glucose.UniRule annotation2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei152 – 1521SubstrateCurated
Binding sitei196 – 1961SubstrateCurated
Binding sitei310 – 3101Substrate; via carbonyl oxygenCurated
Active sitei312 – 3121Proton donor/acceptorUniRule annotation
Active sitei496 – 4961Proton donor/acceptorUniRule annotation
Binding sitei511 – 5111SubstrateCurated

GO - Molecular functioni

  • alpha,alpha-trehalase activity Source: UniProtKB

GO - Biological processi

  • cellular hyperosmotic response Source: EcoCyc
  • cellular response to DNA damage stimulus Source: EcoliWiki
  • trehalose catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciEcoCyc:TREHALAPERI-MONOMER.
ECOL316407:JW1186-MONOMER.
MetaCyc:TREHALAPERI-MONOMER.
BRENDAi3.2.1.28. 2026.

Protein family/group databases

CAZyiGH37. Glycoside Hydrolase Family 37.

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic trehalaseUniRule annotation (EC:3.2.1.28UniRule annotation)
Alternative name(s):
Alpha,alpha-trehalaseUniRule annotation
Alpha,alpha-trehalose glucohydrolaseUniRule annotation
Short name:
Tre37A
Gene namesi
Name:treAUniRule annotation
Synonyms:osmA
Ordered Locus Names:b1197, JW1186
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11017. treA.

Subcellular locationi

GO - Cellular componenti

  • outer membrane-bounded periplasmic space Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3030UniRule annotation1 PublicationAdd
BLAST
Chaini31 – 565535Periplasmic trehalasePRO_0000012041Add
BLAST

Proteomic databases

EPDiP13482.
PaxDbiP13482.
PRIDEiP13482.

Expressioni

Inductioni

By growth at high osmolarity, is regulated by cAMP.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

BioGridi4260095. 121 interactions.
DIPiDIP-11022N.
IntActiP13482. 5 interactions.
STRINGi511145.b1197.

Structurei

Secondary structure

1
565
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi40 – 434Combined sources
Helixi45 – 539Combined sources
Beta strandi57 – 593Combined sources
Helixi61 – 644Combined sources
Beta strandi67 – 704Combined sources
Helixi72 – 8211Combined sources
Helixi90 – 978Combined sources
Helixi115 – 1228Combined sources
Turni123 – 1264Combined sources
Helixi158 – 17013Combined sources
Helixi174 – 19118Combined sources
Beta strandi196 – 1994Combined sources
Helixi200 – 2023Combined sources
Helixi211 – 22212Combined sources
Helixi224 – 24118Combined sources
Turni242 – 2443Combined sources
Helixi245 – 2473Combined sources
Beta strandi252 – 2543Combined sources
Beta strandi257 – 2593Combined sources
Beta strandi265 – 2673Combined sources
Helixi278 – 2803Combined sources
Helixi281 – 2899Combined sources
Helixi296 – 30813Combined sources
Helixi315 – 3173Combined sources
Beta strandi318 – 3203Combined sources
Helixi324 – 3263Combined sources
Helixi329 – 3313Combined sources
Helixi335 – 35521Combined sources
Helixi358 – 37821Combined sources
Beta strandi379 – 3813Combined sources
Turni382 – 3854Combined sources
Turni392 – 3954Combined sources
Helixi403 – 4064Combined sources
Helixi407 – 4104Combined sources
Helixi416 – 42914Combined sources
Beta strandi438 – 4403Combined sources
Beta strandi444 – 4485Combined sources
Helixi454 – 46512Combined sources
Turni466 – 4683Combined sources
Helixi470 – 49122Combined sources
Beta strandi492 – 4965Combined sources
Beta strandi502 – 5043Combined sources
Beta strandi510 – 5123Combined sources
Helixi519 – 53214Combined sources
Beta strandi535 – 5373Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2JF4X-ray2.20A31-565[»]
2JG0X-ray1.50A31-565[»]
2JJBX-ray1.90A/B/C/D31-565[»]
2WYNX-ray2.10A/B/C/D31-565[»]
ProteinModelPortaliP13482.
SMRiP13482. Positions 37-547.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP13482.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni159 – 1602Substrate bindingCurated
Regioni205 – 2073Substrate bindingCurated
Regioni277 – 2793Substrate bindingCurated

Sequence similaritiesi

Belongs to the glycosyl hydrolase 37 family.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105E53. Bacteria.
COG1626. LUCA.
HOGENOMiHOG000215464.
InParanoidiP13482.
KOiK01194.
OMAiWAGQLHQ.
PhylomeDBiP13482.

Family and domain databases

HAMAPiMF_01060. Peripl_trehalase. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR001661. Glyco_hydro_37.
IPR018232. Glyco_hydro_37_CS.
IPR023720. Trehalase_periplasmic.
[Graphical view]
PANTHERiPTHR23403. PTHR23403. 1 hit.
PfamiPF01204. Trehalase. 1 hit.
[Graphical view]
PRINTSiPR00744. GLHYDRLASE37.
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiPS00927. TREHALASE_1. 1 hit.
PS00928. TREHALASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P13482-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSPAPSRPQ KMALIPACIF LCFAALSVQA EETPVTPQPP DILLGPLFND
60 70 80 90 100
VQNAKLFPDQ KTFADAVPNS DPLMILADYR MQQNQSGFDL RHFVNVNFTL
110 120 130 140 150
PKEGEKYVPP EGQSLREHID GLWPVLTRST ENTEKWDSLL PLPEPYVVPG
160 170 180 190 200
GRFREVYYWD SYFTMLGLAE SGHWDKVADM VANFAHEIDT YGHIPNGNRS
210 220 230 240 250
YYLSRSQPPF FALMVELLAQ HEGDAALKQY LPQMQKEYAY WMDGVENLQA
260 270 280 290 300
GQQEKRVVKL QDGTLLNRYW DDRDTPRPES WVEDIATAKS NPNRPATEIY
310 320 330 340 350
RDLRSAAASG WDFSSRWMDN PQQLNTLRTT SIVPVDLNSL MFKMEKILAR
360 370 380 390 400
ASKAAGDNAM ANQYETLANA RQKGIEKYLW NDQQGWYADY DLKSHKVRNQ
410 420 430 440 450
LTAAALFPLY VNAAAKDRAN KMATATKTHL LQPGGLNTTS VKSGQQWDAP
460 470 480 490 500
NGWAPLQWVA TEGLQNYGQK EVAMDISWHF LTNVQHTYDR EKKLVEKYDV
510 520 530 540 550
STTGTGGGGG EYPLQDGFGW TNGVTLKMLD LICPKEQPCD NVPATRPTVK
560
SATTQPSTKE AQPTP
Length:565
Mass (Da):63,637
Last modified:January 1, 1990 - v1
Checksum:i4885D7556A3C0781
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15868 Genomic DNA. Translation: CAA33878.1.
U00096 Genomic DNA. Translation: AAC74281.1.
AP009048 Genomic DNA. Translation: BAA36054.1.
PIRiS04782.
RefSeqiNP_415715.1. NC_000913.3.
WP_000841714.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74281; AAC74281; b1197.
BAA36054; BAA36054; BAA36054.
GeneIDi945757.
KEGGiecj:JW1186.
eco:b1197.
PATRICi32117642. VBIEscCol129921_1243.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15868 Genomic DNA. Translation: CAA33878.1.
U00096 Genomic DNA. Translation: AAC74281.1.
AP009048 Genomic DNA. Translation: BAA36054.1.
PIRiS04782.
RefSeqiNP_415715.1. NC_000913.3.
WP_000841714.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2JF4X-ray2.20A31-565[»]
2JG0X-ray1.50A31-565[»]
2JJBX-ray1.90A/B/C/D31-565[»]
2WYNX-ray2.10A/B/C/D31-565[»]
ProteinModelPortaliP13482.
SMRiP13482. Positions 37-547.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260095. 121 interactions.
DIPiDIP-11022N.
IntActiP13482. 5 interactions.
STRINGi511145.b1197.

Protein family/group databases

CAZyiGH37. Glycoside Hydrolase Family 37.

Proteomic databases

EPDiP13482.
PaxDbiP13482.
PRIDEiP13482.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74281; AAC74281; b1197.
BAA36054; BAA36054; BAA36054.
GeneIDi945757.
KEGGiecj:JW1186.
eco:b1197.
PATRICi32117642. VBIEscCol129921_1243.

Organism-specific databases

EchoBASEiEB1010.
EcoGeneiEG11017. treA.

Phylogenomic databases

eggNOGiENOG4105E53. Bacteria.
COG1626. LUCA.
HOGENOMiHOG000215464.
InParanoidiP13482.
KOiK01194.
OMAiWAGQLHQ.
PhylomeDBiP13482.

Enzyme and pathway databases

BioCyciEcoCyc:TREHALAPERI-MONOMER.
ECOL316407:JW1186-MONOMER.
MetaCyc:TREHALAPERI-MONOMER.
BRENDAi3.2.1.28. 2026.

Miscellaneous databases

EvolutionaryTraceiP13482.
PROiP13482.

Family and domain databases

HAMAPiMF_01060. Peripl_trehalase. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR001661. Glyco_hydro_37.
IPR018232. Glyco_hydro_37_CS.
IPR023720. Trehalase_periplasmic.
[Graphical view]
PANTHERiPTHR23403. PTHR23403. 1 hit.
PfamiPF01204. Trehalase. 1 hit.
[Graphical view]
PRINTSiPR00744. GLHYDRLASE37.
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiPS00927. TREHALASE_1. 1 hit.
PS00928. TREHALASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTREA_ECOLI
AccessioniPrimary (citable) accession number: P13482
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: September 7, 2016
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.