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P13479 (IMM9_ECOLX) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Colicin-E9 immunity protein
Alternative name(s):
ImmE9
Microcin-E9 immunity protein
Gene names
Name:imm
Synonyms:ceiE9
Encoded onPlasmid ColE9-J
OrganismEscherichia coli
Taxonomic identifier562 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length86 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This protein is able to protect a cell, which harbors the plasmid ColE9 encoding colicin E9, against colicin E9, it binds specifically to the DNase-type colicin and inhibits its bactericidal activity.

Sequence similarities

Belongs to the colicins ColE2/ColE8/ColE9 and pyocins S1/S2 family.

Ontologies

Keywords
   Biological processBacteriocin immunity
   Technical term3D-structure
Plasmid
Gene Ontology (GO)
   Biological_processbacteriocin immunity

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functiontoxic substance binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 8686Colicin-E9 immunity protein
PRO_0000218711

Experimental info

Sequence conflict261D → N in AAA23076. Ref.3

Secondary structure

............. 86
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P13479 [UniParc].

Last modified April 1, 1990. Version 2.
Checksum: 136CBD25D071FDB4

FASTA869,583
        10         20         30         40         50         60 
MELKHSISDY TEAEFLQLVT TICNADTSSE EELVKLVTHF EEMTEHPSGS DLIYYPKEGD 

        70         80 
DDSPSGIVNT VKQWRAANGK SGFKQG 

« Hide

References

[1]"Nucleotide sequences from the colicin E5, E6 and E9 operons: presence of a degenerate transposon-like structure in the ColE9-J plasmid."
Lau P.C.K., Condie J.A.
Mol. Gen. Genet. 217:269-277(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-9.
[2]Lau P.C.K.
Submitted (DEC-1989) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Nucleotide sequence of the immunity and lysis region of the ColE9-J plasmid."
James R., Jarvis M., Barker D.F.
J. Gen. Microbiol. 133:1553-1562(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"Three-dimensional solution structure and 13C nuclear magnetic resonance assignments of the colicin E9 immunity protein Im9."
Osborne M.J., Breeze A.L., Lian L.Y., Reilly A., James R., Kleanthous C., Moore G.R.
Biochemistry 35:9505-9512(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X15858 Genomic DNA. Translation: CAA33863.1.
M16803 Genomic DNA. Translation: AAA23076.1.
PIRA32535.
RefSeqYP_002533538.1. NC_011977.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1BXIX-ray2.05A1-86[»]
1E0HNMR-A1-86[»]
1EMVX-ray1.70A1-86[»]
1FR2X-ray1.60A1-86[»]
1IMPNMR-A1-86[»]
1IMQNMR-A1-86[»]
2GYKX-ray1.60A/E1-86[»]
2GZEX-ray1.80A1-86[»]
2GZFX-ray1.75A1-86[»]
2GZGX-ray1.70A1-86[»]
2GZIX-ray1.70A1-86[»]
2GZJX-ray1.60A/E1-86[»]
2K5XNMR-A1-86[»]
2VLNX-ray1.60A1-86[»]
2VLOX-ray1.80A1-86[»]
2VLPX-ray2.00A1-86[»]
2VLQX-ray1.60A1-86[»]
3GJNX-ray2.48A/D1-86[»]
3GKLX-ray2.20C/D1-86[»]
ProteinModelPortalP13479.
SMRP13479. Positions 1-86.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-16991N.
IntActP13479. 1 interaction.
MINTMINT-1771493.

Proteomic databases

PRIDEP13479.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID7377405.

Family and domain databases

Gene3D1.10.1200.20. 1 hit.
InterProIPR023802. Colicin/pyocin_immunity_dom.
IPR000290. Colicin_pyocin.
[Graphical view]
PfamPF01320. Colicin_Pyocin. 1 hit.
[Graphical view]
PRINTSPR01299. PYOCIN.
ProDomPD007225. Colicin_pyocin. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMSSF47345. SSF47345. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP13479.

Entry information

Entry nameIMM9_ECOLX
AccessionPrimary (citable) accession number: P13479
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: April 1, 1990
Last modified: October 16, 2013
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references