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P13479

- IMM9_ECOLX

UniProt

P13479 - IMM9_ECOLX

Protein

Colicin-E9 immunity protein

Gene

imm

Organism
Escherichia coli
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
  1. Functioni

    This protein is able to protect a cell, which harbors the plasmid ColE9 encoding colicin E9, against colicin E9, it binds specifically to the DNase-type colicin and inhibits its bactericidal activity.

    GO - Molecular functioni

    1. toxic substance binding Source: InterPro

    GO - Biological processi

    1. bacteriocin immunity Source: UniProtKB-KW

    Keywords - Biological processi

    Bacteriocin immunity

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Colicin-E9 immunity protein
    Alternative name(s):
    ImmE9
    Microcin-E9 immunity protein
    Gene namesi
    Name:imm
    Synonyms:ceiE9
    Encoded oniPlasmid ColE9-J0 Publication
    OrganismiEscherichia coli
    Taxonomic identifieri562 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 8686Colicin-E9 immunity proteinPRO_0000218711Add
    BLAST

    Proteomic databases

    PRIDEiP13479.

    Interactioni

    Protein-protein interaction databases

    DIPiDIP-16991N.
    IntActiP13479. 1 interaction.
    MINTiMINT-1771493.

    Structurei

    Secondary structure

    1
    86
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi7 – 93
    Helixi12 – 2312
    Beta strandi27 – 293
    Helixi30 – 4415
    Turni47 – 504
    Helixi51 – 544
    Helixi64 – 7714

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1BXIX-ray2.05A1-86[»]
    1E0HNMR-A1-86[»]
    1EMVX-ray1.70A1-86[»]
    1FR2X-ray1.60A1-86[»]
    1IMPNMR-A1-86[»]
    1IMQNMR-A1-86[»]
    2GYKX-ray1.60A/E1-86[»]
    2GZEX-ray1.80A1-86[»]
    2GZFX-ray1.75A1-86[»]
    2GZGX-ray1.70A1-86[»]
    2GZIX-ray1.70A1-86[»]
    2GZJX-ray1.60A/E1-86[»]
    2K5XNMR-A1-86[»]
    2VLNX-ray1.60A1-86[»]
    2VLOX-ray1.80A1-86[»]
    2VLPX-ray2.00A1-86[»]
    2VLQX-ray1.60A1-86[»]
    3GJNX-ray2.48A/D1-86[»]
    3GKLX-ray2.20C/D1-86[»]
    ProteinModelPortaliP13479.
    SMRiP13479. Positions 1-86.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP13479.

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    Gene3Di1.10.1200.20. 1 hit.
    InterProiIPR023802. Colicin/pyocin_immunity_dom.
    IPR000290. Colicin_pyocin.
    [Graphical view]
    PfamiPF01320. Colicin_Pyocin. 1 hit.
    [Graphical view]
    PRINTSiPR01299. PYOCIN.
    ProDomiPD007225. Colicin_pyocin. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SUPFAMiSSF47345. SSF47345. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P13479-1 [UniParc]FASTAAdd to Basket

    « Hide

    MELKHSISDY TEAEFLQLVT TICNADTSSE EELVKLVTHF EEMTEHPSGS   50
    DLIYYPKEGD DDSPSGIVNT VKQWRAANGK SGFKQG 86
    Length:86
    Mass (Da):9,583
    Last modified:April 1, 1990 - v2
    Checksum:i136CBD25D071FDB4
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti26 – 261D → N in AAA23076. (PubMed:3312476)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X15858 Genomic DNA. Translation: CAA33863.1.
    M16803 Genomic DNA. Translation: AAA23076.1.
    PIRiA32535.
    RefSeqiWP_012644887.1. NC_011977.1.
    YP_002533538.1. NC_011977.1.

    Genome annotation databases

    GeneIDi7377405.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X15858 Genomic DNA. Translation: CAA33863.1 .
    M16803 Genomic DNA. Translation: AAA23076.1 .
    PIRi A32535.
    RefSeqi WP_012644887.1. NC_011977.1.
    YP_002533538.1. NC_011977.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1BXI X-ray 2.05 A 1-86 [» ]
    1E0H NMR - A 1-86 [» ]
    1EMV X-ray 1.70 A 1-86 [» ]
    1FR2 X-ray 1.60 A 1-86 [» ]
    1IMP NMR - A 1-86 [» ]
    1IMQ NMR - A 1-86 [» ]
    2GYK X-ray 1.60 A/E 1-86 [» ]
    2GZE X-ray 1.80 A 1-86 [» ]
    2GZF X-ray 1.75 A 1-86 [» ]
    2GZG X-ray 1.70 A 1-86 [» ]
    2GZI X-ray 1.70 A 1-86 [» ]
    2GZJ X-ray 1.60 A/E 1-86 [» ]
    2K5X NMR - A 1-86 [» ]
    2VLN X-ray 1.60 A 1-86 [» ]
    2VLO X-ray 1.80 A 1-86 [» ]
    2VLP X-ray 2.00 A 1-86 [» ]
    2VLQ X-ray 1.60 A 1-86 [» ]
    3GJN X-ray 2.48 A/D 1-86 [» ]
    3GKL X-ray 2.20 C/D 1-86 [» ]
    ProteinModelPortali P13479.
    SMRi P13479. Positions 1-86.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-16991N.
    IntActi P13479. 1 interaction.
    MINTi MINT-1771493.

    Proteomic databases

    PRIDEi P13479.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 7377405.

    Miscellaneous databases

    EvolutionaryTracei P13479.

    Family and domain databases

    Gene3Di 1.10.1200.20. 1 hit.
    InterProi IPR023802. Colicin/pyocin_immunity_dom.
    IPR000290. Colicin_pyocin.
    [Graphical view ]
    Pfami PF01320. Colicin_Pyocin. 1 hit.
    [Graphical view ]
    PRINTSi PR01299. PYOCIN.
    ProDomi PD007225. Colicin_pyocin. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SUPFAMi SSF47345. SSF47345. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequences from the colicin E5, E6 and E9 operons: presence of a degenerate transposon-like structure in the ColE9-J plasmid."
      Lau P.C.K., Condie J.A.
      Mol. Gen. Genet. 217:269-277(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-9.
    2. Lau P.C.K.
      Submitted (DEC-1989) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Nucleotide sequence of the immunity and lysis region of the ColE9-J plasmid."
      James R., Jarvis M., Barker D.F.
      J. Gen. Microbiol. 133:1553-1562(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. "Three-dimensional solution structure and 13C nuclear magnetic resonance assignments of the colicin E9 immunity protein Im9."
      Osborne M.J., Breeze A.L., Lian L.Y., Reilly A., James R., Kleanthous C., Moore G.R.
      Biochemistry 35:9505-9512(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR.

    Entry informationi

    Entry nameiIMM9_ECOLX
    AccessioniPrimary (citable) accession number: P13479
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: April 1, 1990
    Last modified: October 1, 2014
    This is version 80 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Plasmid

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3