ID GTFC_STRMU Reviewed; 1455 AA. AC P13470; O69382; O69385; O69388; O69391; O69397; P05427; DT 01-JAN-1990, integrated into UniProtKB/Swiss-Prot. DT 28-NOV-2002, sequence version 2. DT 27-MAR-2024, entry version 172. DE RecName: Full=Glucosyltransferase-SI; DE Short=GTF-SI; DE EC=2.4.1.5; DE AltName: Full=Dextransucrase; DE AltName: Full=Sucrose 6-glucosyltransferase; DE Flags: Precursor; GN Name=gtfC; OrderedLocusNames=SMU_1005; OS Streptococcus mutans serotype c (strain ATCC 700610 / UA159). OC Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae; OC Streptococcus. OX NCBI_TaxID=210007; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=GS-5; RX PubMed=2976010; DOI=10.1016/0378-1119(88)90382-4; RA Ueda S., Shiroza T., Kuramitsu H.K.; RT "Sequence analysis of the gtfC gene from Streptococcus mutans GS-5."; RL Gene 69:101-109(1988). RN [2] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=MT4239 / Serotype c, MT4245 / Serotype e, MT4251 / Serotype f, RC MT4467 / Serotype e, and MT8148 / Serotype c; RX PubMed=9570124; DOI=10.1111/j.1574-6968.1998.tb12965.x; RA Fujiwara T., Terao Y., Hoshino T., Kawabata S., Ooshima T., Sobue S., RA Kimura S., Hamada S.; RT "Molecular analyses of glucosyltransferase genes among strains of RT Streptococcus mutans."; RL FEMS Microbiol. Lett. 161:331-336(1998). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 700610 / UA159; RX PubMed=12397186; DOI=10.1073/pnas.172501299; RA Ajdic D.J., McShan W.M., McLaughlin R.E., Savic G., Chang J., Carson M.B., RA Primeaux C., Tian R., Kenton S., Jia H.G., Lin S.P., Qian Y., Li S., RA Zhu H., Najar F.Z., Lai H., White J., Roe B.A., Ferretti J.J.; RT "Genome sequence of Streptococcus mutans UA159, a cariogenic dental RT pathogen."; RL Proc. Natl. Acad. Sci. U.S.A. 99:14434-14439(2002). RN [4] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-349. RC STRAIN=GS-5; RX PubMed=3040685; DOI=10.1128/jb.169.9.4263-4270.1987; RA Shiroza T., Ueda S., Kuramitsu H.K.; RT "Sequence analysis of the gtfB gene from Streptococcus mutans."; RL J. Bacteriol. 169:4263-4270(1987). CC -!- FUNCTION: Production of extracellular glucans, that are thought to play CC a key role in the development of the dental plaque because of their CC ability to adhere to smooth surfaces and mediate the aggregation of CC bacterial cells and food debris. CC -!- CATALYTIC ACTIVITY: CC Reaction=[(1->6)-alpha-D-glucosyl](n) + sucrose = [(1->6)-alpha-D- CC glucosyl](n+1) + D-fructose; Xref=Rhea:RHEA:18825, Rhea:RHEA- CC COMP:11144, Rhea:RHEA-COMP:11145, ChEBI:CHEBI:17992, CC ChEBI:CHEBI:18269, ChEBI:CHEBI:37721; EC=2.4.1.5; CC -!- SUBCELLULAR LOCATION: Secreted. CC -!- MISCELLANEOUS: GTF-I synthesizes water-insoluble glucans (alpha 1,3- CC linked glucose and some 1,6 linkages), GTF-S synthesizes water-soluble CC glucans (alpha 1,6-glucose). GTF-SI synthesizes both forms of glucans. CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 70 family. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; M22054; AAA88592.1; -; Genomic_DNA. DR EMBL; D88652; BAA26102.1; -; Genomic_DNA. DR EMBL; D88655; BAA26106.1; -; Genomic_DNA. DR EMBL; D88658; BAA26110.1; -; Genomic_DNA. DR EMBL; D88661; BAA26114.1; -; Genomic_DNA. DR EMBL; D89978; BAA26120.1; -; Genomic_DNA. DR EMBL; AE014133; AAN58706.1; -; Genomic_DNA. DR EMBL; M17361; AAA88589.1; -; Genomic_DNA. DR PIR; JT0345; JT0345. DR RefSeq; NP_721400.1; NC_004350.2. DR RefSeq; WP_002352269.1; NC_004350.2. DR PDB; 3AIB; X-ray; 3.09 A; A/B/C/D/E/F/G/H=244-1087. DR PDB; 3AIC; X-ray; 3.11 A; A/B/C/D/E/F/G/H=244-1087. DR PDB; 3AIE; X-ray; 2.10 A; A/B/C/D/E/F/G/H=244-1087. DR PDBsum; 3AIB; -. DR PDBsum; 3AIC; -. DR PDBsum; 3AIE; -. DR AlphaFoldDB; P13470; -. DR SMR; P13470; -. DR STRING; 210007.SMU_1005; -. DR ChEMBL; CHEMBL2366466; -. DR CAZy; GH70; Glycoside Hydrolase Family 70. DR KEGG; smu:SMU_1005; -. DR PATRIC; fig|210007.7.peg.898; -. DR eggNOG; COG0366; Bacteria. DR eggNOG; COG5263; Bacteria. DR HOGENOM; CLU_001623_1_0_9; -. DR OrthoDB; 2032428at2; -. DR PhylomeDB; P13470; -. DR SABIO-RK; P13470; -. DR EvolutionaryTrace; P13470; -. DR PHI-base; PHI:7054; -. DR Proteomes; UP000002512; Chromosome. DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell. DR GO; GO:0047849; F:dextransucrase activity; IEA:UniProtKB-EC. DR GO; GO:0046527; F:glucosyltransferase activity; IEA:InterPro. DR GO; GO:0009250; P:glucan biosynthetic process; IEA:InterPro. DR Gene3D; 2.10.270.10; Cholin Binding; 4. DR Gene3D; 3.20.20.470; Glucansucrase; 1. DR Gene3D; 2.30.30.420; glucansucrase; 1. DR InterPro; IPR018337; Cell_wall/Cho-bd_repeat. DR InterPro; IPR027636; Glucan-bd_rpt. DR InterPro; IPR003318; Glyco_hydro70cat. DR InterPro; IPR017853; Glycoside_hydrolase_SF. DR InterPro; IPR022263; KxYKxGKxW. DR NCBIfam; TIGR04035; glucan_65_rpt; 4. DR NCBIfam; TIGR03715; KxYKxGKxW; 1. DR Pfam; PF01473; Choline_bind_1; 2. DR Pfam; PF19127; Choline_bind_3; 4. DR Pfam; PF02324; Glyco_hydro_70; 1. DR Pfam; PF19258; KxYKxGKxW_sig; 1. DR SUPFAM; SSF51445; (Trans)glycosidases; 2. DR SUPFAM; SSF69360; Cell wall binding repeat; 3. DR PROSITE; PS51170; CW; 11. PE 1: Evidence at protein level; KW 3D-structure; Dental caries; Glycosyltransferase; Reference proteome; KW Repeat; Secreted; Signal; Transferase. FT SIGNAL 1..34 FT CHAIN 35..1455 FT /note="Glucosyltransferase-SI" FT /id="PRO_0000021386" FT REPEAT 184..203 FT /note="Cell wall-binding 1" FT REPEAT 205..224 FT /note="Cell wall-binding 2" FT REPEAT 1116..1135 FT /note="Cell wall-binding 3" FT REPEAT 1136..1155 FT /note="Cell wall-binding 4" FT REPEAT 1219..1239 FT /note="Cell wall-binding 5" FT REPEAT 1242..1262 FT /note="Cell wall-binding 6" FT REPEAT 1263..1282 FT /note="Cell wall-binding 7" FT REPEAT 1307..1327 FT /note="Cell wall-binding 8" FT REPEAT 1328..1347 FT /note="Cell wall-binding 9" FT REPEAT 1372..1392 FT /note="Cell wall-binding 10" FT REPEAT 1393..1412 FT /note="Cell wall-binding 11" FT REGION 64..121 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT REGION 250..1050 FT /note="Catalytic; approximate" FT VARIANT 21 FT /note="V -> I (in strain: GS-5)" FT VARIANT 81 FT /note="P -> L (in strain: MT4239)" FT VARIANT 106 FT /note="D -> V (in strain: GS-5)" FT VARIANT 116 FT /note="S -> A (in strain: GS-5 and MT4467)" FT VARIANT 126 FT /note="A -> T (in strain: GS-5)" FT VARIANT 150..151 FT /note="SR -> PK (in strain: GS-5, MT4239 and MT4467)" FT VARIANT 256 FT /note="A -> V (in strain: GS-5 and MT4467)" FT VARIANT 425 FT /note="R -> N (in strain: MT4251)" FT VARIANT 519 FT /note="Y -> D (in strain: MT4245 and MT4251)" FT VARIANT 538 FT /note="R -> K (in strain: MT4245 and MT4251)" FT VARIANT 545 FT /note="Y -> F (in strain: MT4245 and MT4251)" FT VARIANT 597 FT /note="N -> D (in strain: MT4245, MT4251, MT4467 and FT MT8148)" FT VARIANT 600 FT /note="R -> K (in strain: MT4245, MT4251, MT4467 and FT MT8148)" FT VARIANT 601 FT /note="A -> T (in strain: GS-5)" FT VARIANT 614 FT /note="M -> T (in strain: GS-5)" FT VARIANT 727 FT /note="T -> I (in strain: MT8148)" FT VARIANT 734 FT /note="A -> V (in strain: MT8148)" FT VARIANT 964 FT /note="L -> F (in strain: MT4239)" FT VARIANT 1113 FT /note="N -> Y (in strain: MT4239)" FT VARIANT 1118 FT /note="A -> T (in strain: MT4239)" FT VARIANT 1204 FT /note="I -> V (in strain: GS-5, MT4239, MT4467 and MT8148)" FT VARIANT 1208 FT /note="V -> I (in strain: MT8148)" FT VARIANT 1292..1294 FT /note="DGH -> NGY (in strain: GS-5, MT4467 and sMT8148)" FT VARIANT 1305..1369 FT /note="Missing (in strain: MT4245)" FT VARIANT 1326 FT /note="I -> V (in strain: MT8148)" FT VARIANT 1331 FT /note="T -> A (in strain: GS-5, MT4239, MT4467 and MT8148)" FT VARIANT 1377 FT /note="R -> K (in strain: MT8148)" FT VARIANT 1398 FT /note="V -> I (in strain: MT8148)" FT VARIANT 1424 FT /note="D -> N (in strain: MT4239)" FT VARIANT 1439 FT /note="V -> I (in strain: MT4239 and MT8148)" FT VARIANT 1444 FT /note="S -> P (in strain: MT8148)" FT CONFLICT 1337..1455 FT /note="QRLYFKSNGVQAKGELITERKGRIKYYDPNSGNEVRNRYVRTSSGNWYYFGN FT DGYALIGWHVVEGRRVYFDENGVYRYASHDQRNHWDYDYRRDFGRGSSSAVRFRHSRNG FT FFDNFFRF -> HASILSLMVFRLRESSLQSVKVVSNTMILIPEMKFVIVM (in FT Ref. 1; AAA88592/BAA26102)" FT /evidence="ECO:0000305" FT HELIX 246..249 FT /evidence="ECO:0007829|PDB:3AIE" FT STRAND 250..252 FT /evidence="ECO:0007829|PDB:3AIE" FT HELIX 256..258 FT /evidence="ECO:0007829|PDB:3AIE" FT STRAND 274..279 FT /evidence="ECO:0007829|PDB:3AIE" FT TURN 280..282 FT /evidence="ECO:0007829|PDB:3AIE" FT STRAND 283..286 FT /evidence="ECO:0007829|PDB:3AIE" FT HELIX 295..297 FT /evidence="ECO:0007829|PDB:3AIE" FT HELIX 303..316 FT /evidence="ECO:0007829|PDB:3AIE" FT HELIX 329..350 FT /evidence="ECO:0007829|PDB:3AIE" FT HELIX 354..366 FT /evidence="ECO:0007829|PDB:3AIE" FT HELIX 368..370 FT /evidence="ECO:0007829|PDB:3AIE" FT HELIX 372..375 FT /evidence="ECO:0007829|PDB:3AIE" FT STRAND 386..389 FT /evidence="ECO:0007829|PDB:3AIE" FT HELIX 394..399 FT /evidence="ECO:0007829|PDB:3AIE" FT TURN 411..414 FT /evidence="ECO:0007829|PDB:3AIE" FT STRAND 434..438 FT /evidence="ECO:0007829|PDB:3AIE" FT HELIX 443..457 FT /evidence="ECO:0007829|PDB:3AIE" FT HELIX 459..464 FT /evidence="ECO:0007829|PDB:3AIE" FT HELIX 467..469 FT /evidence="ECO:0007829|PDB:3AIB" FT STRAND 473..476 FT /evidence="ECO:0007829|PDB:3AIE" FT HELIX 479..481 FT /evidence="ECO:0007829|PDB:3AIE" FT HELIX 485..498 FT /evidence="ECO:0007829|PDB:3AIE" FT TURN 500..502 FT /evidence="ECO:0007829|PDB:3AIE" FT HELIX 504..508 FT /evidence="ECO:0007829|PDB:3AIE" FT HELIX 521..528 FT /evidence="ECO:0007829|PDB:3AIE" FT STRAND 530..535 FT /evidence="ECO:0007829|PDB:3AIE" FT HELIX 537..546 FT /evidence="ECO:0007829|PDB:3AIE" FT HELIX 551..553 FT /evidence="ECO:0007829|PDB:3AIE" FT HELIX 559..562 FT /evidence="ECO:0007829|PDB:3AIE" FT STRAND 563..566 FT /evidence="ECO:0007829|PDB:3AIE" FT STRAND 569..571 FT /evidence="ECO:0007829|PDB:3AIE" FT STRAND 580..582 FT /evidence="ECO:0007829|PDB:3AIE" FT TURN 589..591 FT /evidence="ECO:0007829|PDB:3AIE" FT HELIX 592..602 FT /evidence="ECO:0007829|PDB:3AIE" FT HELIX 614..629 FT /evidence="ECO:0007829|PDB:3AIE" FT STRAND 634..636 FT /evidence="ECO:0007829|PDB:3AIE" FT HELIX 640..647 FT /evidence="ECO:0007829|PDB:3AIE" FT STRAND 651..658 FT /evidence="ECO:0007829|PDB:3AIE" FT HELIX 659..662 FT /evidence="ECO:0007829|PDB:3AIE" FT STRAND 665..668 FT /evidence="ECO:0007829|PDB:3AIE" FT TURN 669..671 FT /evidence="ECO:0007829|PDB:3AIC" FT HELIX 677..690 FT /evidence="ECO:0007829|PDB:3AIE" FT STRAND 695..702 FT /evidence="ECO:0007829|PDB:3AIE" FT STRAND 705..712 FT /evidence="ECO:0007829|PDB:3AIE" FT HELIX 726..729 FT /evidence="ECO:0007829|PDB:3AIE" FT STRAND 733..738 FT /evidence="ECO:0007829|PDB:3AIE" FT STRAND 751..754 FT /evidence="ECO:0007829|PDB:3AIE" FT HELIX 757..759 FT /evidence="ECO:0007829|PDB:3AIE" FT STRAND 763..770 FT /evidence="ECO:0007829|PDB:3AIE" FT STRAND 772..777 FT /evidence="ECO:0007829|PDB:3AIE" FT HELIX 782..787 FT /evidence="ECO:0007829|PDB:3AIE" FT STRAND 795..799 FT /evidence="ECO:0007829|PDB:3AIE" FT TURN 801..803 FT /evidence="ECO:0007829|PDB:3AIE" FT STRAND 807..811 FT /evidence="ECO:0007829|PDB:3AIE" FT STRAND 813..821 FT /evidence="ECO:0007829|PDB:3AIE" FT HELIX 849..852 FT /evidence="ECO:0007829|PDB:3AIE" FT STRAND 856..858 FT /evidence="ECO:0007829|PDB:3AIE" FT HELIX 870..872 FT /evidence="ECO:0007829|PDB:3AIE" FT HELIX 874..880 FT /evidence="ECO:0007829|PDB:3AIE" FT HELIX 882..887 FT /evidence="ECO:0007829|PDB:3AIE" FT STRAND 892..894 FT /evidence="ECO:0007829|PDB:3AIE" FT HELIX 908..910 FT /evidence="ECO:0007829|PDB:3AIE" FT TURN 911..913 FT /evidence="ECO:0007829|PDB:3AIE" FT STRAND 915..919 FT /evidence="ECO:0007829|PDB:3AIE" FT STRAND 924..928 FT /evidence="ECO:0007829|PDB:3AIC" FT HELIX 935..947 FT /evidence="ECO:0007829|PDB:3AIE" FT STRAND 951..956 FT /evidence="ECO:0007829|PDB:3AIE" FT STRAND 959..961 FT /evidence="ECO:0007829|PDB:3AIE" FT STRAND 965..975 FT /evidence="ECO:0007829|PDB:3AIE" FT STRAND 988..997 FT /evidence="ECO:0007829|PDB:3AIE" FT STRAND 1000..1002 FT /evidence="ECO:0007829|PDB:3AIE" FT HELIX 1003..1007 FT /evidence="ECO:0007829|PDB:3AIE" FT TURN 1008..1011 FT /evidence="ECO:0007829|PDB:3AIE" FT HELIX 1012..1018 FT /evidence="ECO:0007829|PDB:3AIE" FT HELIX 1020..1024 FT /evidence="ECO:0007829|PDB:3AIE" FT TURN 1028..1030 FT /evidence="ECO:0007829|PDB:3AIE" FT HELIX 1045..1047 FT /evidence="ECO:0007829|PDB:3AIE" FT STRAND 1048..1052 FT /evidence="ECO:0007829|PDB:3AIE" FT TURN 1065..1068 FT /evidence="ECO:0007829|PDB:3AIE" FT HELIX 1075..1077 FT /evidence="ECO:0007829|PDB:3AIB" FT HELIX 1082..1085 FT /evidence="ECO:0007829|PDB:3AIE" SQ SEQUENCE 1455 AA; 162966 MW; 3CB455A99A4FEC86 CRC64; MEKKVRFKLR KVKKRWVTVS VASAVVTLTS LSGSLVKADS TDDRQQAVTE SQASLVTTSE AAKETLTATD TSTATSATSQ PTATVTDNVS TTNQSTNTTA NTANFDVKPT TTSEQSKTDN SDKIIATSKA VNRLTATGKF VPANNNTAHS RTVTDKIVPI KPKIGKLKQP SSLSQDDIAA LGNVKNIRKV NGKYYYYKED GTLQKNYALN INGKTFFFDE TGALSNNTLP SKKGNITNND NTNSFAQYNQ VYSTDAANFE HVDHYLTAES WYRPKYILKD GKTWTQSTEK DFRPLLMTWW PDQETQRQYV NYMNAQLGIH QTYNTATSPL QLNLAAQTIQ TKIEEKITAE KNTNWLRQTI SAFVKTQSAW NSDSEKPFDD HLQKGALLYS NNSKLTSQAN SNYRILNRTP TNQTGKKDPR YTADRTIGGY EFLLANDVDN SNPVVQAEQL NWLHFLMNFG NIYANDPDAN FDSIRVDAVD NVDADLLQIA GDYLKAAKGI HKNDKAANDH LSILEAWSYN DTPYLHDDGD NMINMDNRLR LSLLYSLAKP LNQRSGMNPL ITNSLVNRTD DNAETAAVPS YSFIRAHDSE VQDLIRNIIR AEINPNVVGY SFTMEEIKKA FEIYNKDLLA TEKKYTHYNT ALSYALLLTN KSSVPRVYYG DMFTDDGQYM AHKTINYEAI ETLLKARIKY VSGGQAMRNQ QVGNSEIITS VRYGKGALKA TDTGDRTTRT SGVAVIEGNN PSLRLKASDR VVVNMGAAHK NQAYRPLLLT TDNGIKAYHS DQEAAGLVRY TNDRGELIFT AADIKGYANP QVSGYLGVWV PVGAAADQDV RVAASTAPST DGKSVHQNAA LDSRVMFEGF SNFQAFATKK EEYTNVVIAK NVDKFAEWGV TDFEMAPQYV SSTDGSFLDS VIQNGYAFTD RYDLGISKPN KYGTADDLVK AIKALHSKGI KVMADWVPDQ MYALPEKEVV TATRVDKYGT PVAGSQIKNT LYVVDGKSSG KDQQAKYGGA FLEELQAKYP ELFARKQIST GVPMDPSVKI KQWSAKYFNG TNILGRGAGY VLKDQATNTY FSLVSDNTFL PKSLVNPNHG TSSSVTGLVF DGKGYVYYST SGNQAKNAFI SLGNNWYYFD NNGYMVTGAQ SINGANYYFL SNGIQLRNAI YDNGNKVLSY YGNDGRRYEN GYYLFGQQWR YFQNGIMAVG LTRIHGAVQY FDASGFQAKG QFITTADGKL RYFDRDSGNQ ISNRFVRNSK GEWFLFDHNG VAVTGTVTFN GQRLYFKPNG VQAKGEFIRD ADGHLRYYDP NSGNEVRNRF VRNSKGEWFL FDHNGIAVTG TRVVNGQRLY FKSNGVQAKG ELITERKGRI KYYDPNSGNE VRNRYVRTSS GNWYYFGNDG YALIGWHVVE GRRVYFDENG VYRYASHDQR NHWDYDYRRD FGRGSSSAVR FRHSRNGFFD NFFRF //