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P13470

- GTFC_STRMU

UniProt

P13470 - GTFC_STRMU

Protein

Glucosyltransferase-SI

Gene

gtfC

Organism
Streptococcus mutans serotype c (strain ATCC 700610 / UA159)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 130 (01 Oct 2014)
      Sequence version 2 (28 Nov 2002)
      Previous versions | rss
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    Functioni

    Production of extracellular glucans, that are thought to play a key role in the development of the dental plaque because of their ability to adhere to smooth surfaces and mediate the aggregation of bacterial cells and food debris.

    Catalytic activityi

    Sucrose + ((1->6)-alpha-D-glucosyl)(n) = D-fructose + ((1->6)-alpha-D-glucosyl)(n+1).

    GO - Molecular functioni

    1. dextransucrase activity Source: UniProtKB-EC
    2. glucosyltransferase activity Source: InterPro

    GO - Biological processi

    1. glucan biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    BioCyciSMUT210007:GC7Z-956-MONOMER.
    SABIO-RKP13470.

    Protein family/group databases

    CAZyiGH70. Glycoside Hydrolase Family 70.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glucosyltransferase-SI (EC:2.4.1.5)
    Short name:
    GTF-SI
    Alternative name(s):
    Dextransucrase
    Sucrose 6-glucosyltransferase
    Gene namesi
    Name:gtfC
    Ordered Locus Names:SMU_1005
    OrganismiStreptococcus mutans serotype c (strain ATCC 700610 / UA159)
    Taxonomic identifieri210007 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
    ProteomesiUP000002512: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Keywords - Diseasei

    Dental caries

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3434Add
    BLAST
    Chaini35 – 14551421Glucosyltransferase-SIPRO_0000021386Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi210007.SMU.1005.

    Structurei

    Secondary structure

    1
    1455
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi246 – 2494
    Beta strandi250 – 2523
    Helixi256 – 2583
    Beta strandi274 – 2796
    Turni280 – 2823
    Beta strandi283 – 2864
    Helixi295 – 2973
    Helixi303 – 31614
    Helixi329 – 35022
    Helixi354 – 36613
    Helixi368 – 3703
    Helixi372 – 3754
    Beta strandi386 – 3894
    Helixi394 – 3996
    Turni411 – 4144
    Beta strandi434 – 4385
    Helixi443 – 45715
    Helixi459 – 4646
    Helixi467 – 4693
    Beta strandi473 – 4764
    Helixi479 – 4813
    Helixi485 – 49814
    Turni500 – 5023
    Helixi504 – 5085
    Helixi521 – 5288
    Beta strandi530 – 5356
    Helixi537 – 54610
    Helixi551 – 5533
    Helixi559 – 5624
    Beta strandi563 – 5664
    Beta strandi569 – 5713
    Beta strandi580 – 5823
    Turni589 – 5913
    Helixi592 – 60211
    Helixi614 – 62916
    Beta strandi634 – 6363
    Helixi640 – 6478
    Beta strandi651 – 6588
    Helixi659 – 6624
    Beta strandi665 – 6684
    Turni669 – 6713
    Helixi677 – 69014
    Beta strandi695 – 7028
    Beta strandi705 – 7128
    Helixi726 – 7294
    Beta strandi733 – 7386
    Beta strandi751 – 7544
    Helixi757 – 7593
    Beta strandi763 – 7708
    Beta strandi772 – 7776
    Helixi782 – 7876
    Beta strandi795 – 7995
    Turni801 – 8033
    Beta strandi807 – 8115
    Beta strandi813 – 8219
    Helixi849 – 8524
    Beta strandi856 – 8583
    Helixi870 – 8723
    Helixi874 – 8807
    Helixi882 – 8876
    Beta strandi892 – 8943
    Helixi908 – 9103
    Turni911 – 9133
    Beta strandi915 – 9195
    Beta strandi924 – 9285
    Helixi935 – 94713
    Beta strandi951 – 9566
    Beta strandi959 – 9613
    Beta strandi965 – 97511
    Beta strandi988 – 99710
    Beta strandi1000 – 10023
    Helixi1003 – 10075
    Turni1008 – 10114
    Helixi1012 – 10187
    Helixi1020 – 10245
    Turni1028 – 10303
    Helixi1045 – 10473
    Beta strandi1048 – 10525
    Turni1065 – 10684
    Helixi1075 – 10773
    Helixi1082 – 10854

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3AIBX-ray3.09A/B/C/D/E/F/G/H244-1087[»]
    3AICX-ray3.11A/B/C/D/E/F/G/H244-1087[»]
    3AIEX-ray2.10A/B/C/D/E/F/G/H244-1087[»]
    ProteinModelPortaliP13470.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP13470.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati184 – 20320Cell wall-binding 1Add
    BLAST
    Repeati205 – 22420Cell wall-binding 2Add
    BLAST
    Repeati1116 – 113520Cell wall-binding 3Add
    BLAST
    Repeati1136 – 115520Cell wall-binding 4Add
    BLAST
    Repeati1219 – 123921Cell wall-binding 5Add
    BLAST
    Repeati1242 – 126221Cell wall-binding 6Add
    BLAST
    Repeati1263 – 128220Cell wall-binding 7Add
    BLAST
    Repeati1307 – 132721Cell wall-binding 8Add
    BLAST
    Repeati1328 – 134720Cell wall-binding 9Add
    BLAST
    Repeati1372 – 139221Cell wall-binding 10Add
    BLAST
    Repeati1393 – 141220Cell wall-binding 11Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni250 – 1050801Catalytic; approximateAdd
    BLAST

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 70 family.Curated
    Contains 11 cell wall-binding repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiCOG5263.
    KOiK00689.
    OMAiNSKGEWF.
    OrthoDBiEOG60PH6T.
    PhylomeDBiP13470.

    Family and domain databases

    Gene3Di3.20.20.80. 4 hits.
    InterProiIPR018337. Cell_wall/Cho-bd_repeat.
    IPR027636. Glucan-bd_rpt.
    IPR003318. Glyco_hydro70cat.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR022263. KxYKxGKxW.
    [Graphical view]
    PfamiPF01473. CW_binding_1. 2 hits.
    PF02324. Glyco_hydro_70. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 4 hits.
    TIGRFAMsiTIGR04035. glucan_65_rpt. 4 hits.
    TIGR03715. KxYKxGKxW. 1 hit.
    PROSITEiPS51170. CW. 11 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P13470-1 [UniParc]FASTAAdd to Basket

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    MEKKVRFKLR KVKKRWVTVS VASAVVTLTS LSGSLVKADS TDDRQQAVTE     50
    SQASLVTTSE AAKETLTATD TSTATSATSQ PTATVTDNVS TTNQSTNTTA 100
    NTANFDVKPT TTSEQSKTDN SDKIIATSKA VNRLTATGKF VPANNNTAHS 150
    RTVTDKIVPI KPKIGKLKQP SSLSQDDIAA LGNVKNIRKV NGKYYYYKED 200
    GTLQKNYALN INGKTFFFDE TGALSNNTLP SKKGNITNND NTNSFAQYNQ 250
    VYSTDAANFE HVDHYLTAES WYRPKYILKD GKTWTQSTEK DFRPLLMTWW 300
    PDQETQRQYV NYMNAQLGIH QTYNTATSPL QLNLAAQTIQ TKIEEKITAE 350
    KNTNWLRQTI SAFVKTQSAW NSDSEKPFDD HLQKGALLYS NNSKLTSQAN 400
    SNYRILNRTP TNQTGKKDPR YTADRTIGGY EFLLANDVDN SNPVVQAEQL 450
    NWLHFLMNFG NIYANDPDAN FDSIRVDAVD NVDADLLQIA GDYLKAAKGI 500
    HKNDKAANDH LSILEAWSYN DTPYLHDDGD NMINMDNRLR LSLLYSLAKP 550
    LNQRSGMNPL ITNSLVNRTD DNAETAAVPS YSFIRAHDSE VQDLIRNIIR 600
    AEINPNVVGY SFTMEEIKKA FEIYNKDLLA TEKKYTHYNT ALSYALLLTN 650
    KSSVPRVYYG DMFTDDGQYM AHKTINYEAI ETLLKARIKY VSGGQAMRNQ 700
    QVGNSEIITS VRYGKGALKA TDTGDRTTRT SGVAVIEGNN PSLRLKASDR 750
    VVVNMGAAHK NQAYRPLLLT TDNGIKAYHS DQEAAGLVRY TNDRGELIFT 800
    AADIKGYANP QVSGYLGVWV PVGAAADQDV RVAASTAPST DGKSVHQNAA 850
    LDSRVMFEGF SNFQAFATKK EEYTNVVIAK NVDKFAEWGV TDFEMAPQYV 900
    SSTDGSFLDS VIQNGYAFTD RYDLGISKPN KYGTADDLVK AIKALHSKGI 950
    KVMADWVPDQ MYALPEKEVV TATRVDKYGT PVAGSQIKNT LYVVDGKSSG 1000
    KDQQAKYGGA FLEELQAKYP ELFARKQIST GVPMDPSVKI KQWSAKYFNG 1050
    TNILGRGAGY VLKDQATNTY FSLVSDNTFL PKSLVNPNHG TSSSVTGLVF 1100
    DGKGYVYYST SGNQAKNAFI SLGNNWYYFD NNGYMVTGAQ SINGANYYFL 1150
    SNGIQLRNAI YDNGNKVLSY YGNDGRRYEN GYYLFGQQWR YFQNGIMAVG 1200
    LTRIHGAVQY FDASGFQAKG QFITTADGKL RYFDRDSGNQ ISNRFVRNSK 1250
    GEWFLFDHNG VAVTGTVTFN GQRLYFKPNG VQAKGEFIRD ADGHLRYYDP 1300
    NSGNEVRNRF VRNSKGEWFL FDHNGIAVTG TRVVNGQRLY FKSNGVQAKG 1350
    ELITERKGRI KYYDPNSGNE VRNRYVRTSS GNWYYFGNDG YALIGWHVVE 1400
    GRRVYFDENG VYRYASHDQR NHWDYDYRRD FGRGSSSAVR FRHSRNGFFD 1450
    NFFRF 1455
    Length:1,455
    Mass (Da):162,966
    Last modified:November 28, 2002 - v2
    Checksum:i3CB455A99A4FEC86
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti1337 – 1455119QRLYF…NFFRF → HASILSLMVFRLRESSLQSV KVVSNTMILIPEMKFVIVM in AAA88592. (PubMed:2976010)CuratedAdd
    BLAST
    Sequence conflicti1337 – 1455119QRLYF…NFFRF → HASILSLMVFRLRESSLQSV KVVSNTMILIPEMKFVIVM in BAA26102. (PubMed:2976010)CuratedAdd
    BLAST

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti21 – 211V → I in strain: GS-5.
    Natural varianti81 – 811P → L in strain: MT4239.
    Natural varianti106 – 1061D → V in strain: GS-5.
    Natural varianti116 – 1161S → A in strain: GS-5 and MT4467.
    Natural varianti126 – 1261A → T in strain: GS-5.
    Natural varianti150 – 1512SR → PK in strain: GS-5, MT4239 and MT4467.
    Natural varianti256 – 2561A → V in strain: GS-5 and MT4467.
    Natural varianti425 – 4251R → N in strain: MT4251.
    Natural varianti519 – 5191Y → D in strain: MT4245 and MT4251.
    Natural varianti538 – 5381R → K in strain: MT4245 and MT4251.
    Natural varianti545 – 5451Y → F in strain: MT4245 and MT4251.
    Natural varianti597 – 5971N → D in strain: MT4245, MT4251, MT4467 and MT8148.
    Natural varianti600 – 6001R → K in strain: MT4245, MT4251, MT4467 and MT8148.
    Natural varianti601 – 6011A → T in strain: GS-5.
    Natural varianti614 – 6141M → T in strain: GS-5.
    Natural varianti727 – 7271T → I in strain: MT8148.
    Natural varianti734 – 7341A → V in strain: MT8148.
    Natural varianti964 – 9641L → F in strain: MT4239.
    Natural varianti1113 – 11131N → Y in strain: MT4239.
    Natural varianti1118 – 11181A → T in strain: MT4239.
    Natural varianti1204 – 12041I → V in strain: GS-5, MT4239, MT4467 and MT8148.
    Natural varianti1208 – 12081V → I in strain: MT8148.
    Natural varianti1292 – 12943DGH → NGY in strain: GS-5, MT4467 and sMT8148.
    Natural varianti1305 – 136965Missing in strain: MT4245.
    Add
    BLAST
    Natural varianti1326 – 13261I → V in strain: MT8148.
    Natural varianti1331 – 13311T → A in strain: GS-5, MT4239, MT4467 and MT8148.
    Natural varianti1377 – 13771R → K in strain: MT8148.
    Natural varianti1398 – 13981V → I in strain: MT8148.
    Natural varianti1424 – 14241D → N in strain: MT4239.
    Natural varianti1439 – 14391V → I in strain: MT4239 and MT8148.
    Natural varianti1444 – 14441S → P in strain: MT8148.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M22054 Genomic DNA. Translation: AAA88592.1.
    D88652 Genomic DNA. Translation: BAA26102.1.
    D88655 Genomic DNA. Translation: BAA26106.1.
    D88658 Genomic DNA. Translation: BAA26110.1.
    D88661 Genomic DNA. Translation: BAA26114.1.
    D89978 Genomic DNA. Translation: BAA26120.1.
    AE014133 Genomic DNA. Translation: AAN58706.1.
    M17361 Genomic DNA. Translation: AAA88589.1.
    PIRiJT0345.
    RefSeqiNP_721400.1. NC_004350.2.

    Genome annotation databases

    EnsemblBacteriaiAAN58706; AAN58706; SMU_1005.
    GeneIDi1028343.
    KEGGismu:SMU_1005.
    PATRICi19664131. VBIStrMut61772_0898.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M22054 Genomic DNA. Translation: AAA88592.1 .
    D88652 Genomic DNA. Translation: BAA26102.1 .
    D88655 Genomic DNA. Translation: BAA26106.1 .
    D88658 Genomic DNA. Translation: BAA26110.1 .
    D88661 Genomic DNA. Translation: BAA26114.1 .
    D89978 Genomic DNA. Translation: BAA26120.1 .
    AE014133 Genomic DNA. Translation: AAN58706.1 .
    M17361 Genomic DNA. Translation: AAA88589.1 .
    PIRi JT0345.
    RefSeqi NP_721400.1. NC_004350.2.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3AIB X-ray 3.09 A/B/C/D/E/F/G/H 244-1087 [» ]
    3AIC X-ray 3.11 A/B/C/D/E/F/G/H 244-1087 [» ]
    3AIE X-ray 2.10 A/B/C/D/E/F/G/H 244-1087 [» ]
    ProteinModelPortali P13470.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 210007.SMU.1005.

    Protein family/group databases

    CAZyi GH70. Glycoside Hydrolase Family 70.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAN58706 ; AAN58706 ; SMU_1005 .
    GeneIDi 1028343.
    KEGGi smu:SMU_1005.
    PATRICi 19664131. VBIStrMut61772_0898.

    Phylogenomic databases

    eggNOGi COG5263.
    KOi K00689.
    OMAi NSKGEWF.
    OrthoDBi EOG60PH6T.
    PhylomeDBi P13470.

    Enzyme and pathway databases

    BioCyci SMUT210007:GC7Z-956-MONOMER.
    SABIO-RK P13470.

    Miscellaneous databases

    EvolutionaryTracei P13470.

    Family and domain databases

    Gene3Di 3.20.20.80. 4 hits.
    InterProi IPR018337. Cell_wall/Cho-bd_repeat.
    IPR027636. Glucan-bd_rpt.
    IPR003318. Glyco_hydro70cat.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR022263. KxYKxGKxW.
    [Graphical view ]
    Pfami PF01473. CW_binding_1. 2 hits.
    PF02324. Glyco_hydro_70. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 4 hits.
    TIGRFAMsi TIGR04035. glucan_65_rpt. 4 hits.
    TIGR03715. KxYKxGKxW. 1 hit.
    PROSITEi PS51170. CW. 11 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence analysis of the gtfC gene from Streptococcus mutans GS-5."
      Ueda S., Shiroza T., Kuramitsu H.K.
      Gene 69:101-109(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: GS-5.
    2. "Molecular analyses of glucosyltransferase genes among strains of Streptococcus mutans."
      Fujiwara T., Terao Y., Hoshino T., Kawabata S., Ooshima T., Sobue S., Kimura S., Hamada S.
      FEMS Microbiol. Lett. 161:331-336(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: MT4239 / Serotype c, MT4245 / Serotype e, MT4251 / Serotype f, MT4467 / Serotype e and MT8148 / Serotype c.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700610 / UA159.
    4. "Sequence analysis of the gtfB gene from Streptococcus mutans."
      Shiroza T., Ueda S., Kuramitsu H.K.
      J. Bacteriol. 169:4263-4270(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-349.
      Strain: GS-5.

    Entry informationi

    Entry nameiGTFC_STRMU
    AccessioniPrimary (citable) accession number: P13470
    Secondary accession number(s): O69382
    , O69385, O69388, O69391, O69397, P05427
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: November 28, 2002
    Last modified: October 1, 2014
    This is version 130 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    GTF-I synthesizes water-insoluble glucans (alpha 1,3-linked glucose and some 1,6 linkages), GTF-S synthesizes water-soluble glucans (alpha 1,6-glucose). GTF-SI synthesizes both forms of glucans.

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3