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P13470

- GTFC_STRMU

UniProt

P13470 - GTFC_STRMU

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Protein

Glucosyltransferase-SI

Gene

gtfC

Organism
Streptococcus mutans serotype c (strain ATCC 700610 / UA159)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Production of extracellular glucans, that are thought to play a key role in the development of the dental plaque because of their ability to adhere to smooth surfaces and mediate the aggregation of bacterial cells and food debris.

Catalytic activityi

Sucrose + ((1->6)-alpha-D-glucosyl)(n) = D-fructose + ((1->6)-alpha-D-glucosyl)(n+1).

GO - Molecular functioni

  1. dextransucrase activity Source: UniProtKB-EC
  2. glucosyltransferase activity Source: InterPro

GO - Biological processi

  1. glucan biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciSMUT210007:GC7Z-956-MONOMER.

Protein family/group databases

CAZyiGH70. Glycoside Hydrolase Family 70.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucosyltransferase-SI (EC:2.4.1.5)
Short name:
GTF-SI
Alternative name(s):
Dextransucrase
Sucrose 6-glucosyltransferase
Gene namesi
Name:gtfC
Ordered Locus Names:SMU_1005
OrganismiStreptococcus mutans serotype c (strain ATCC 700610 / UA159)
Taxonomic identifieri210007 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
ProteomesiUP000002512: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Keywords - Diseasei

Dental caries

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3434Add
BLAST
Chaini35 – 14551421Glucosyltransferase-SIPRO_0000021386Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi210007.SMU.1005.

Structurei

Secondary structure

1
1455
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi246 – 2494Combined sources
Beta strandi250 – 2523Combined sources
Helixi256 – 2583Combined sources
Beta strandi274 – 2796Combined sources
Turni280 – 2823Combined sources
Beta strandi283 – 2864Combined sources
Helixi295 – 2973Combined sources
Helixi303 – 31614Combined sources
Helixi329 – 35022Combined sources
Helixi354 – 36613Combined sources
Helixi368 – 3703Combined sources
Helixi372 – 3754Combined sources
Beta strandi386 – 3894Combined sources
Helixi394 – 3996Combined sources
Turni411 – 4144Combined sources
Beta strandi434 – 4385Combined sources
Helixi443 – 45715Combined sources
Helixi459 – 4646Combined sources
Helixi467 – 4693Combined sources
Beta strandi473 – 4764Combined sources
Helixi479 – 4813Combined sources
Helixi485 – 49814Combined sources
Turni500 – 5023Combined sources
Helixi504 – 5085Combined sources
Helixi521 – 5288Combined sources
Beta strandi530 – 5356Combined sources
Helixi537 – 54610Combined sources
Helixi551 – 5533Combined sources
Helixi559 – 5624Combined sources
Beta strandi563 – 5664Combined sources
Beta strandi569 – 5713Combined sources
Beta strandi580 – 5823Combined sources
Turni589 – 5913Combined sources
Helixi592 – 60211Combined sources
Helixi614 – 62916Combined sources
Beta strandi634 – 6363Combined sources
Helixi640 – 6478Combined sources
Beta strandi651 – 6588Combined sources
Helixi659 – 6624Combined sources
Beta strandi665 – 6684Combined sources
Turni669 – 6713Combined sources
Helixi677 – 69014Combined sources
Beta strandi695 – 7028Combined sources
Beta strandi705 – 7128Combined sources
Helixi726 – 7294Combined sources
Beta strandi733 – 7386Combined sources
Beta strandi751 – 7544Combined sources
Helixi757 – 7593Combined sources
Beta strandi763 – 7708Combined sources
Beta strandi772 – 7776Combined sources
Helixi782 – 7876Combined sources
Beta strandi795 – 7995Combined sources
Turni801 – 8033Combined sources
Beta strandi807 – 8115Combined sources
Beta strandi813 – 8219Combined sources
Helixi849 – 8524Combined sources
Beta strandi856 – 8583Combined sources
Helixi870 – 8723Combined sources
Helixi874 – 8807Combined sources
Helixi882 – 8876Combined sources
Beta strandi892 – 8943Combined sources
Helixi908 – 9103Combined sources
Turni911 – 9133Combined sources
Beta strandi915 – 9195Combined sources
Beta strandi924 – 9285Combined sources
Helixi935 – 94713Combined sources
Beta strandi951 – 9566Combined sources
Beta strandi959 – 9613Combined sources
Beta strandi965 – 97511Combined sources
Beta strandi988 – 99710Combined sources
Beta strandi1000 – 10023Combined sources
Helixi1003 – 10075Combined sources
Turni1008 – 10114Combined sources
Helixi1012 – 10187Combined sources
Helixi1020 – 10245Combined sources
Turni1028 – 10303Combined sources
Helixi1045 – 10473Combined sources
Beta strandi1048 – 10525Combined sources
Turni1065 – 10684Combined sources
Helixi1075 – 10773Combined sources
Helixi1082 – 10854Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3AIBX-ray3.09A/B/C/D/E/F/G/H244-1087[»]
3AICX-ray3.11A/B/C/D/E/F/G/H244-1087[»]
3AIEX-ray2.10A/B/C/D/E/F/G/H244-1087[»]
ProteinModelPortaliP13470.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP13470.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati184 – 20320Cell wall-binding 1Add
BLAST
Repeati205 – 22420Cell wall-binding 2Add
BLAST
Repeati1116 – 113520Cell wall-binding 3Add
BLAST
Repeati1136 – 115520Cell wall-binding 4Add
BLAST
Repeati1219 – 123921Cell wall-binding 5Add
BLAST
Repeati1242 – 126221Cell wall-binding 6Add
BLAST
Repeati1263 – 128220Cell wall-binding 7Add
BLAST
Repeati1307 – 132721Cell wall-binding 8Add
BLAST
Repeati1328 – 134720Cell wall-binding 9Add
BLAST
Repeati1372 – 139221Cell wall-binding 10Add
BLAST
Repeati1393 – 141220Cell wall-binding 11Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni250 – 1050801Catalytic; approximateAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 70 family.Curated
Contains 11 cell wall-binding repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiCOG5263.
KOiK00689.
OMAiNSKGEWF.
OrthoDBiEOG60PH6T.
PhylomeDBiP13470.

Family and domain databases

Gene3Di3.20.20.80. 4 hits.
InterProiIPR018337. Cell_wall/Cho-bd_repeat.
IPR027636. Glucan-bd_rpt.
IPR003318. Glyco_hydro70cat.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR022263. KxYKxGKxW.
[Graphical view]
PfamiPF01473. CW_binding_1. 2 hits.
PF02324. Glyco_hydro_70. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 4 hits.
TIGRFAMsiTIGR04035. glucan_65_rpt. 4 hits.
TIGR03715. KxYKxGKxW. 1 hit.
PROSITEiPS51170. CW. 11 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P13470-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEKKVRFKLR KVKKRWVTVS VASAVVTLTS LSGSLVKADS TDDRQQAVTE
60 70 80 90 100
SQASLVTTSE AAKETLTATD TSTATSATSQ PTATVTDNVS TTNQSTNTTA
110 120 130 140 150
NTANFDVKPT TTSEQSKTDN SDKIIATSKA VNRLTATGKF VPANNNTAHS
160 170 180 190 200
RTVTDKIVPI KPKIGKLKQP SSLSQDDIAA LGNVKNIRKV NGKYYYYKED
210 220 230 240 250
GTLQKNYALN INGKTFFFDE TGALSNNTLP SKKGNITNND NTNSFAQYNQ
260 270 280 290 300
VYSTDAANFE HVDHYLTAES WYRPKYILKD GKTWTQSTEK DFRPLLMTWW
310 320 330 340 350
PDQETQRQYV NYMNAQLGIH QTYNTATSPL QLNLAAQTIQ TKIEEKITAE
360 370 380 390 400
KNTNWLRQTI SAFVKTQSAW NSDSEKPFDD HLQKGALLYS NNSKLTSQAN
410 420 430 440 450
SNYRILNRTP TNQTGKKDPR YTADRTIGGY EFLLANDVDN SNPVVQAEQL
460 470 480 490 500
NWLHFLMNFG NIYANDPDAN FDSIRVDAVD NVDADLLQIA GDYLKAAKGI
510 520 530 540 550
HKNDKAANDH LSILEAWSYN DTPYLHDDGD NMINMDNRLR LSLLYSLAKP
560 570 580 590 600
LNQRSGMNPL ITNSLVNRTD DNAETAAVPS YSFIRAHDSE VQDLIRNIIR
610 620 630 640 650
AEINPNVVGY SFTMEEIKKA FEIYNKDLLA TEKKYTHYNT ALSYALLLTN
660 670 680 690 700
KSSVPRVYYG DMFTDDGQYM AHKTINYEAI ETLLKARIKY VSGGQAMRNQ
710 720 730 740 750
QVGNSEIITS VRYGKGALKA TDTGDRTTRT SGVAVIEGNN PSLRLKASDR
760 770 780 790 800
VVVNMGAAHK NQAYRPLLLT TDNGIKAYHS DQEAAGLVRY TNDRGELIFT
810 820 830 840 850
AADIKGYANP QVSGYLGVWV PVGAAADQDV RVAASTAPST DGKSVHQNAA
860 870 880 890 900
LDSRVMFEGF SNFQAFATKK EEYTNVVIAK NVDKFAEWGV TDFEMAPQYV
910 920 930 940 950
SSTDGSFLDS VIQNGYAFTD RYDLGISKPN KYGTADDLVK AIKALHSKGI
960 970 980 990 1000
KVMADWVPDQ MYALPEKEVV TATRVDKYGT PVAGSQIKNT LYVVDGKSSG
1010 1020 1030 1040 1050
KDQQAKYGGA FLEELQAKYP ELFARKQIST GVPMDPSVKI KQWSAKYFNG
1060 1070 1080 1090 1100
TNILGRGAGY VLKDQATNTY FSLVSDNTFL PKSLVNPNHG TSSSVTGLVF
1110 1120 1130 1140 1150
DGKGYVYYST SGNQAKNAFI SLGNNWYYFD NNGYMVTGAQ SINGANYYFL
1160 1170 1180 1190 1200
SNGIQLRNAI YDNGNKVLSY YGNDGRRYEN GYYLFGQQWR YFQNGIMAVG
1210 1220 1230 1240 1250
LTRIHGAVQY FDASGFQAKG QFITTADGKL RYFDRDSGNQ ISNRFVRNSK
1260 1270 1280 1290 1300
GEWFLFDHNG VAVTGTVTFN GQRLYFKPNG VQAKGEFIRD ADGHLRYYDP
1310 1320 1330 1340 1350
NSGNEVRNRF VRNSKGEWFL FDHNGIAVTG TRVVNGQRLY FKSNGVQAKG
1360 1370 1380 1390 1400
ELITERKGRI KYYDPNSGNE VRNRYVRTSS GNWYYFGNDG YALIGWHVVE
1410 1420 1430 1440 1450
GRRVYFDENG VYRYASHDQR NHWDYDYRRD FGRGSSSAVR FRHSRNGFFD

NFFRF
Length:1,455
Mass (Da):162,966
Last modified:November 28, 2002 - v2
Checksum:i3CB455A99A4FEC86
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1337 – 1455119QRLYF…NFFRF → HASILSLMVFRLRESSLQSV KVVSNTMILIPEMKFVIVM in AAA88592. (PubMed:2976010)CuratedAdd
BLAST
Sequence conflicti1337 – 1455119QRLYF…NFFRF → HASILSLMVFRLRESSLQSV KVVSNTMILIPEMKFVIVM in BAA26102. (PubMed:2976010)CuratedAdd
BLAST

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti21 – 211V → I in strain: GS-5.
Natural varianti81 – 811P → L in strain: MT4239.
Natural varianti106 – 1061D → V in strain: GS-5.
Natural varianti116 – 1161S → A in strain: GS-5 and MT4467.
Natural varianti126 – 1261A → T in strain: GS-5.
Natural varianti150 – 1512SR → PK in strain: GS-5, MT4239 and MT4467.
Natural varianti256 – 2561A → V in strain: GS-5 and MT4467.
Natural varianti425 – 4251R → N in strain: MT4251.
Natural varianti519 – 5191Y → D in strain: MT4245 and MT4251.
Natural varianti538 – 5381R → K in strain: MT4245 and MT4251.
Natural varianti545 – 5451Y → F in strain: MT4245 and MT4251.
Natural varianti597 – 5971N → D in strain: MT4245, MT4251, MT4467 and MT8148.
Natural varianti600 – 6001R → K in strain: MT4245, MT4251, MT4467 and MT8148.
Natural varianti601 – 6011A → T in strain: GS-5.
Natural varianti614 – 6141M → T in strain: GS-5.
Natural varianti727 – 7271T → I in strain: MT8148.
Natural varianti734 – 7341A → V in strain: MT8148.
Natural varianti964 – 9641L → F in strain: MT4239.
Natural varianti1113 – 11131N → Y in strain: MT4239.
Natural varianti1118 – 11181A → T in strain: MT4239.
Natural varianti1204 – 12041I → V in strain: GS-5, MT4239, MT4467 and MT8148.
Natural varianti1208 – 12081V → I in strain: MT8148.
Natural varianti1292 – 12943DGH → NGY in strain: GS-5, MT4467 and sMT8148.
Natural varianti1305 – 136965Missing in strain: MT4245.
Add
BLAST
Natural varianti1326 – 13261I → V in strain: MT8148.
Natural varianti1331 – 13311T → A in strain: GS-5, MT4239, MT4467 and MT8148.
Natural varianti1377 – 13771R → K in strain: MT8148.
Natural varianti1398 – 13981V → I in strain: MT8148.
Natural varianti1424 – 14241D → N in strain: MT4239.
Natural varianti1439 – 14391V → I in strain: MT4239 and MT8148.
Natural varianti1444 – 14441S → P in strain: MT8148.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22054 Genomic DNA. Translation: AAA88592.1.
D88652 Genomic DNA. Translation: BAA26102.1.
D88655 Genomic DNA. Translation: BAA26106.1.
D88658 Genomic DNA. Translation: BAA26110.1.
D88661 Genomic DNA. Translation: BAA26114.1.
D89978 Genomic DNA. Translation: BAA26120.1.
AE014133 Genomic DNA. Translation: AAN58706.1.
M17361 Genomic DNA. Translation: AAA88589.1.
PIRiJT0345.
RefSeqiNP_721400.1. NC_004350.2.

Genome annotation databases

EnsemblBacteriaiAAN58706; AAN58706; SMU_1005.
GeneIDi1028343.
KEGGismu:SMU_1005.
PATRICi19664131. VBIStrMut61772_0898.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22054 Genomic DNA. Translation: AAA88592.1 .
D88652 Genomic DNA. Translation: BAA26102.1 .
D88655 Genomic DNA. Translation: BAA26106.1 .
D88658 Genomic DNA. Translation: BAA26110.1 .
D88661 Genomic DNA. Translation: BAA26114.1 .
D89978 Genomic DNA. Translation: BAA26120.1 .
AE014133 Genomic DNA. Translation: AAN58706.1 .
M17361 Genomic DNA. Translation: AAA88589.1 .
PIRi JT0345.
RefSeqi NP_721400.1. NC_004350.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3AIB X-ray 3.09 A/B/C/D/E/F/G/H 244-1087 [» ]
3AIC X-ray 3.11 A/B/C/D/E/F/G/H 244-1087 [» ]
3AIE X-ray 2.10 A/B/C/D/E/F/G/H 244-1087 [» ]
ProteinModelPortali P13470.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 210007.SMU.1005.

Chemistry

ChEMBLi CHEMBL2366466.

Protein family/group databases

CAZyi GH70. Glycoside Hydrolase Family 70.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAN58706 ; AAN58706 ; SMU_1005 .
GeneIDi 1028343.
KEGGi smu:SMU_1005.
PATRICi 19664131. VBIStrMut61772_0898.

Phylogenomic databases

eggNOGi COG5263.
KOi K00689.
OMAi NSKGEWF.
OrthoDBi EOG60PH6T.
PhylomeDBi P13470.

Enzyme and pathway databases

BioCyci SMUT210007:GC7Z-956-MONOMER.

Miscellaneous databases

EvolutionaryTracei P13470.

Family and domain databases

Gene3Di 3.20.20.80. 4 hits.
InterProi IPR018337. Cell_wall/Cho-bd_repeat.
IPR027636. Glucan-bd_rpt.
IPR003318. Glyco_hydro70cat.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR022263. KxYKxGKxW.
[Graphical view ]
Pfami PF01473. CW_binding_1. 2 hits.
PF02324. Glyco_hydro_70. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 4 hits.
TIGRFAMsi TIGR04035. glucan_65_rpt. 4 hits.
TIGR03715. KxYKxGKxW. 1 hit.
PROSITEi PS51170. CW. 11 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence analysis of the gtfC gene from Streptococcus mutans GS-5."
    Ueda S., Shiroza T., Kuramitsu H.K.
    Gene 69:101-109(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: GS-5.
  2. "Molecular analyses of glucosyltransferase genes among strains of Streptococcus mutans."
    Fujiwara T., Terao Y., Hoshino T., Kawabata S., Ooshima T., Sobue S., Kimura S., Hamada S.
    FEMS Microbiol. Lett. 161:331-336(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: MT4239 / Serotype c, MT4245 / Serotype e, MT4251 / Serotype f, MT4467 / Serotype e and MT8148 / Serotype c.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700610 / UA159.
  4. "Sequence analysis of the gtfB gene from Streptococcus mutans."
    Shiroza T., Ueda S., Kuramitsu H.K.
    J. Bacteriol. 169:4263-4270(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-349.
    Strain: GS-5.

Entry informationi

Entry nameiGTFC_STRMU
AccessioniPrimary (citable) accession number: P13470
Secondary accession number(s): O69382
, O69385, O69388, O69391, O69397, P05427
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: November 28, 2002
Last modified: November 26, 2014
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

GTF-I synthesizes water-insoluble glucans (alpha 1,3-linked glucose and some 1,6 linkages), GTF-S synthesizes water-soluble glucans (alpha 1,6-glucose). GTF-SI synthesizes both forms of glucans.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3