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Protein

Glucosyltransferase-SI

Gene

gtfC

Organism
Streptococcus mutans serotype c (strain ATCC 700610 / UA159)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Production of extracellular glucans, that are thought to play a key role in the development of the dental plaque because of their ability to adhere to smooth surfaces and mediate the aggregation of bacterial cells and food debris.

Catalytic activityi

Sucrose + ((1->6)-alpha-D-glucosyl)(n) = D-fructose + ((1->6)-alpha-D-glucosyl)(n+1).

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

SABIO-RKP13470.

Protein family/group databases

CAZyiGH70. Glycoside Hydrolase Family 70.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucosyltransferase-SI (EC:2.4.1.5)
Short name:
GTF-SI
Alternative name(s):
Dextransucrase
Sucrose 6-glucosyltransferase
Gene namesi
Name:gtfC
Ordered Locus Names:SMU_1005
OrganismiStreptococcus mutans serotype c (strain ATCC 700610 / UA159)
Taxonomic identifieri210007 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
Proteomesi
  • UP000002512 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Keywords - Diseasei

Dental caries

Chemistry databases

ChEMBLiCHEMBL2366466.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 34Add BLAST34
ChainiPRO_000002138635 – 1455Glucosyltransferase-SIAdd BLAST1421

Interactioni

Protein-protein interaction databases

STRINGi210007.SMU_1005.

Structurei

Secondary structure

11455
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi246 – 249Combined sources4
Beta strandi250 – 252Combined sources3
Helixi256 – 258Combined sources3
Beta strandi274 – 279Combined sources6
Turni280 – 282Combined sources3
Beta strandi283 – 286Combined sources4
Helixi295 – 297Combined sources3
Helixi303 – 316Combined sources14
Helixi329 – 350Combined sources22
Helixi354 – 366Combined sources13
Helixi368 – 370Combined sources3
Helixi372 – 375Combined sources4
Beta strandi386 – 389Combined sources4
Helixi394 – 399Combined sources6
Turni411 – 414Combined sources4
Beta strandi434 – 438Combined sources5
Helixi443 – 457Combined sources15
Helixi459 – 464Combined sources6
Helixi467 – 469Combined sources3
Beta strandi473 – 476Combined sources4
Helixi479 – 481Combined sources3
Helixi485 – 498Combined sources14
Turni500 – 502Combined sources3
Helixi504 – 508Combined sources5
Helixi521 – 528Combined sources8
Beta strandi530 – 535Combined sources6
Helixi537 – 546Combined sources10
Helixi551 – 553Combined sources3
Helixi559 – 562Combined sources4
Beta strandi563 – 566Combined sources4
Beta strandi569 – 571Combined sources3
Beta strandi580 – 582Combined sources3
Turni589 – 591Combined sources3
Helixi592 – 602Combined sources11
Helixi614 – 629Combined sources16
Beta strandi634 – 636Combined sources3
Helixi640 – 647Combined sources8
Beta strandi651 – 658Combined sources8
Helixi659 – 662Combined sources4
Beta strandi665 – 668Combined sources4
Turni669 – 671Combined sources3
Helixi677 – 690Combined sources14
Beta strandi695 – 702Combined sources8
Beta strandi705 – 712Combined sources8
Helixi726 – 729Combined sources4
Beta strandi733 – 738Combined sources6
Beta strandi751 – 754Combined sources4
Helixi757 – 759Combined sources3
Beta strandi763 – 770Combined sources8
Beta strandi772 – 777Combined sources6
Helixi782 – 787Combined sources6
Beta strandi795 – 799Combined sources5
Turni801 – 803Combined sources3
Beta strandi807 – 811Combined sources5
Beta strandi813 – 821Combined sources9
Helixi849 – 852Combined sources4
Beta strandi856 – 858Combined sources3
Helixi870 – 872Combined sources3
Helixi874 – 880Combined sources7
Helixi882 – 887Combined sources6
Beta strandi892 – 894Combined sources3
Helixi908 – 910Combined sources3
Turni911 – 913Combined sources3
Beta strandi915 – 919Combined sources5
Beta strandi924 – 928Combined sources5
Helixi935 – 947Combined sources13
Beta strandi951 – 956Combined sources6
Beta strandi959 – 961Combined sources3
Beta strandi965 – 975Combined sources11
Beta strandi988 – 997Combined sources10
Beta strandi1000 – 1002Combined sources3
Helixi1003 – 1007Combined sources5
Turni1008 – 1011Combined sources4
Helixi1012 – 1018Combined sources7
Helixi1020 – 1024Combined sources5
Turni1028 – 1030Combined sources3
Helixi1045 – 1047Combined sources3
Beta strandi1048 – 1052Combined sources5
Turni1065 – 1068Combined sources4
Helixi1075 – 1077Combined sources3
Helixi1082 – 1085Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3AIBX-ray3.09A/B/C/D/E/F/G/H244-1087[»]
3AICX-ray3.11A/B/C/D/E/F/G/H244-1087[»]
3AIEX-ray2.10A/B/C/D/E/F/G/H244-1087[»]
ProteinModelPortaliP13470.
SMRiP13470.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP13470.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati184 – 203Cell wall-binding 1Add BLAST20
Repeati205 – 224Cell wall-binding 2Add BLAST20
Repeati1116 – 1135Cell wall-binding 3Add BLAST20
Repeati1136 – 1155Cell wall-binding 4Add BLAST20
Repeati1219 – 1239Cell wall-binding 5Add BLAST21
Repeati1242 – 1262Cell wall-binding 6Add BLAST21
Repeati1263 – 1282Cell wall-binding 7Add BLAST20
Repeati1307 – 1327Cell wall-binding 8Add BLAST21
Repeati1328 – 1347Cell wall-binding 9Add BLAST20
Repeati1372 – 1392Cell wall-binding 10Add BLAST21
Repeati1393 – 1412Cell wall-binding 11Add BLAST20

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni250 – 1050Catalytic; approximateAdd BLAST801

Sequence similaritiesi

Belongs to the glycosyl hydrolase 70 family.Curated
Contains 11 cell wall-binding repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG4106ISY. Bacteria.
ENOG410ZM8A. LUCA.
KOiK00689.
OMAiNSKGEWF.
PhylomeDBiP13470.

Family and domain databases

Gene3Di3.20.20.80. 4 hits.
InterProiIPR018337. Cell_wall/Cho-bd_repeat.
IPR027636. Glucan-bd_rpt.
IPR003318. Glyco_hydro70cat.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR022263. KxYKxGKxW.
[Graphical view]
PfamiPF01473. CW_binding_1. 2 hits.
PF02324. Glyco_hydro_70. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 4 hits.
TIGRFAMsiTIGR04035. glucan_65_rpt. 4 hits.
TIGR03715. KxYKxGKxW. 1 hit.
PROSITEiPS51170. CW. 11 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P13470-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKKVRFKLR KVKKRWVTVS VASAVVTLTS LSGSLVKADS TDDRQQAVTE
60 70 80 90 100
SQASLVTTSE AAKETLTATD TSTATSATSQ PTATVTDNVS TTNQSTNTTA
110 120 130 140 150
NTANFDVKPT TTSEQSKTDN SDKIIATSKA VNRLTATGKF VPANNNTAHS
160 170 180 190 200
RTVTDKIVPI KPKIGKLKQP SSLSQDDIAA LGNVKNIRKV NGKYYYYKED
210 220 230 240 250
GTLQKNYALN INGKTFFFDE TGALSNNTLP SKKGNITNND NTNSFAQYNQ
260 270 280 290 300
VYSTDAANFE HVDHYLTAES WYRPKYILKD GKTWTQSTEK DFRPLLMTWW
310 320 330 340 350
PDQETQRQYV NYMNAQLGIH QTYNTATSPL QLNLAAQTIQ TKIEEKITAE
360 370 380 390 400
KNTNWLRQTI SAFVKTQSAW NSDSEKPFDD HLQKGALLYS NNSKLTSQAN
410 420 430 440 450
SNYRILNRTP TNQTGKKDPR YTADRTIGGY EFLLANDVDN SNPVVQAEQL
460 470 480 490 500
NWLHFLMNFG NIYANDPDAN FDSIRVDAVD NVDADLLQIA GDYLKAAKGI
510 520 530 540 550
HKNDKAANDH LSILEAWSYN DTPYLHDDGD NMINMDNRLR LSLLYSLAKP
560 570 580 590 600
LNQRSGMNPL ITNSLVNRTD DNAETAAVPS YSFIRAHDSE VQDLIRNIIR
610 620 630 640 650
AEINPNVVGY SFTMEEIKKA FEIYNKDLLA TEKKYTHYNT ALSYALLLTN
660 670 680 690 700
KSSVPRVYYG DMFTDDGQYM AHKTINYEAI ETLLKARIKY VSGGQAMRNQ
710 720 730 740 750
QVGNSEIITS VRYGKGALKA TDTGDRTTRT SGVAVIEGNN PSLRLKASDR
760 770 780 790 800
VVVNMGAAHK NQAYRPLLLT TDNGIKAYHS DQEAAGLVRY TNDRGELIFT
810 820 830 840 850
AADIKGYANP QVSGYLGVWV PVGAAADQDV RVAASTAPST DGKSVHQNAA
860 870 880 890 900
LDSRVMFEGF SNFQAFATKK EEYTNVVIAK NVDKFAEWGV TDFEMAPQYV
910 920 930 940 950
SSTDGSFLDS VIQNGYAFTD RYDLGISKPN KYGTADDLVK AIKALHSKGI
960 970 980 990 1000
KVMADWVPDQ MYALPEKEVV TATRVDKYGT PVAGSQIKNT LYVVDGKSSG
1010 1020 1030 1040 1050
KDQQAKYGGA FLEELQAKYP ELFARKQIST GVPMDPSVKI KQWSAKYFNG
1060 1070 1080 1090 1100
TNILGRGAGY VLKDQATNTY FSLVSDNTFL PKSLVNPNHG TSSSVTGLVF
1110 1120 1130 1140 1150
DGKGYVYYST SGNQAKNAFI SLGNNWYYFD NNGYMVTGAQ SINGANYYFL
1160 1170 1180 1190 1200
SNGIQLRNAI YDNGNKVLSY YGNDGRRYEN GYYLFGQQWR YFQNGIMAVG
1210 1220 1230 1240 1250
LTRIHGAVQY FDASGFQAKG QFITTADGKL RYFDRDSGNQ ISNRFVRNSK
1260 1270 1280 1290 1300
GEWFLFDHNG VAVTGTVTFN GQRLYFKPNG VQAKGEFIRD ADGHLRYYDP
1310 1320 1330 1340 1350
NSGNEVRNRF VRNSKGEWFL FDHNGIAVTG TRVVNGQRLY FKSNGVQAKG
1360 1370 1380 1390 1400
ELITERKGRI KYYDPNSGNE VRNRYVRTSS GNWYYFGNDG YALIGWHVVE
1410 1420 1430 1440 1450
GRRVYFDENG VYRYASHDQR NHWDYDYRRD FGRGSSSAVR FRHSRNGFFD

NFFRF
Length:1,455
Mass (Da):162,966
Last modified:November 28, 2002 - v2
Checksum:i3CB455A99A4FEC86
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1337 – 1455QRLYF…NFFRF → HASILSLMVFRLRESSLQSV KVVSNTMILIPEMKFVIVM in AAA88592 (PubMed:2976010).CuratedAdd BLAST119
Sequence conflicti1337 – 1455QRLYF…NFFRF → HASILSLMVFRLRESSLQSV KVVSNTMILIPEMKFVIVM in BAA26102 (PubMed:2976010).CuratedAdd BLAST119

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti21V → I in strain: GS-5. 1
Natural varianti81P → L in strain: MT4239. 1
Natural varianti106D → V in strain: GS-5. 1
Natural varianti116S → A in strain: GS-5 and MT4467. 1
Natural varianti126A → T in strain: GS-5. 1
Natural varianti150 – 151SR → PK in strain: GS-5, MT4239 and MT4467. 2
Natural varianti256A → V in strain: GS-5 and MT4467. 1
Natural varianti425R → N in strain: MT4251. 1
Natural varianti519Y → D in strain: MT4245 and MT4251. 1
Natural varianti538R → K in strain: MT4245 and MT4251. 1
Natural varianti545Y → F in strain: MT4245 and MT4251. 1
Natural varianti597N → D in strain: MT4245, MT4251, MT4467 and MT8148. 1
Natural varianti600R → K in strain: MT4245, MT4251, MT4467 and MT8148. 1
Natural varianti601A → T in strain: GS-5. 1
Natural varianti614M → T in strain: GS-5. 1
Natural varianti727T → I in strain: MT8148. 1
Natural varianti734A → V in strain: MT8148. 1
Natural varianti964L → F in strain: MT4239. 1
Natural varianti1113N → Y in strain: MT4239. 1
Natural varianti1118A → T in strain: MT4239. 1
Natural varianti1204I → V in strain: GS-5, MT4239, MT4467 and MT8148. 1
Natural varianti1208V → I in strain: MT8148. 1
Natural varianti1292 – 1294DGH → NGY in strain: GS-5, MT4467 and sMT8148. 3
Natural varianti1305 – 1369Missing in strain: MT4245. Add BLAST65
Natural varianti1326I → V in strain: MT8148. 1
Natural varianti1331T → A in strain: GS-5, MT4239, MT4467 and MT8148. 1
Natural varianti1377R → K in strain: MT8148. 1
Natural varianti1398V → I in strain: MT8148. 1
Natural varianti1424D → N in strain: MT4239. 1
Natural varianti1439V → I in strain: MT4239 and MT8148. 1
Natural varianti1444S → P in strain: MT8148. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22054 Genomic DNA. Translation: AAA88592.1.
D88652 Genomic DNA. Translation: BAA26102.1.
D88655 Genomic DNA. Translation: BAA26106.1.
D88658 Genomic DNA. Translation: BAA26110.1.
D88661 Genomic DNA. Translation: BAA26114.1.
D89978 Genomic DNA. Translation: BAA26120.1.
AE014133 Genomic DNA. Translation: AAN58706.1.
M17361 Genomic DNA. Translation: AAA88589.1.
PIRiJT0345.
RefSeqiNP_721400.1. NC_004350.2.
WP_002352269.1. NC_004350.2.

Genome annotation databases

EnsemblBacteriaiAAN58706; AAN58706; SMU_1005.
GeneIDi1028343.
KEGGismu:SMU_1005.
PATRICi19664131. VBIStrMut61772_0898.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22054 Genomic DNA. Translation: AAA88592.1.
D88652 Genomic DNA. Translation: BAA26102.1.
D88655 Genomic DNA. Translation: BAA26106.1.
D88658 Genomic DNA. Translation: BAA26110.1.
D88661 Genomic DNA. Translation: BAA26114.1.
D89978 Genomic DNA. Translation: BAA26120.1.
AE014133 Genomic DNA. Translation: AAN58706.1.
M17361 Genomic DNA. Translation: AAA88589.1.
PIRiJT0345.
RefSeqiNP_721400.1. NC_004350.2.
WP_002352269.1. NC_004350.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3AIBX-ray3.09A/B/C/D/E/F/G/H244-1087[»]
3AICX-ray3.11A/B/C/D/E/F/G/H244-1087[»]
3AIEX-ray2.10A/B/C/D/E/F/G/H244-1087[»]
ProteinModelPortaliP13470.
SMRiP13470.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi210007.SMU_1005.

Chemistry databases

ChEMBLiCHEMBL2366466.

Protein family/group databases

CAZyiGH70. Glycoside Hydrolase Family 70.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAN58706; AAN58706; SMU_1005.
GeneIDi1028343.
KEGGismu:SMU_1005.
PATRICi19664131. VBIStrMut61772_0898.

Phylogenomic databases

eggNOGiENOG4106ISY. Bacteria.
ENOG410ZM8A. LUCA.
KOiK00689.
OMAiNSKGEWF.
PhylomeDBiP13470.

Enzyme and pathway databases

SABIO-RKP13470.

Miscellaneous databases

EvolutionaryTraceiP13470.

Family and domain databases

Gene3Di3.20.20.80. 4 hits.
InterProiIPR018337. Cell_wall/Cho-bd_repeat.
IPR027636. Glucan-bd_rpt.
IPR003318. Glyco_hydro70cat.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR022263. KxYKxGKxW.
[Graphical view]
PfamiPF01473. CW_binding_1. 2 hits.
PF02324. Glyco_hydro_70. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 4 hits.
TIGRFAMsiTIGR04035. glucan_65_rpt. 4 hits.
TIGR03715. KxYKxGKxW. 1 hit.
PROSITEiPS51170. CW. 11 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGTFC_STRMU
AccessioniPrimary (citable) accession number: P13470
Secondary accession number(s): O69382
, O69385, O69388, O69391, O69397, P05427
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: November 28, 2002
Last modified: November 2, 2016
This is version 140 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

GTF-I synthesizes water-insoluble glucans (alpha 1,3-linked glucose and some 1,6 linkages), GTF-S synthesizes water-soluble glucans (alpha 1,6-glucose). GTF-SI synthesizes both forms of glucans.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.