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Protein

Nuclease SbcCD subunit C

Gene

sbcC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'->5' double strand exonuclease that can open hairpins. It also has a 5' single-strand endonuclease activity.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi37 – 44ATPSequence analysis8

GO - Molecular functioni

  • 3'-5'-exodeoxyribonuclease activity Source: EcoCyc
  • ATP binding Source: UniProtKB-KW
  • endonuclease activity Source: UniProtKB-KW

GO - Biological processi

  • cellular response to DNA damage stimulus Source: EcoCyc
  • DNA replication Source: UniProtKB-KW
  • recombinational repair Source: EcoCyc

Keywordsi

Molecular functionEndonuclease, Exonuclease, Hydrolase, Nuclease
Biological processDNA recombination, DNA replication
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10927-MONOMER.
MetaCyc:EG10927-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclease SbcCD subunit C
Gene namesi
Name:sbcC
Synonyms:rmuA
Ordered Locus Names:b0397, JW0387
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10927. sbcC.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001058641 – 1048Nuclease SbcCD subunit CAdd BLAST1048

Proteomic databases

PaxDbiP13458.
PRIDEiP13458.

Interactioni

Subunit structurei

Heterodimer of SbcC and SbcD.

Binary interactionsi

WithEntry#Exp.IntActNotes
ynbCP760922EBI-546919,EBI-544837

Protein-protein interaction databases

BioGridi4259821. 33 interactors.
DIPiDIP-342N.
IntActiP13458. 6 interactors.
MINTiMINT-1301497.
STRINGi511145.b0397.

Structurei

3D structure databases

ProteinModelPortaliP13458.
SMRiP13458.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili191 – 402Sequence analysisAdd BLAST212
Coiled coili438 – 502Sequence analysisAdd BLAST65
Coiled coili528 – 648Sequence analysisAdd BLAST121
Coiled coili671 – 695Sequence analysisAdd BLAST25
Coiled coili770 – 880Sequence analysisAdd BLAST111

Sequence similaritiesi

Belongs to the SMC family. SbcC subfamily.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG4105EYH. Bacteria.
COG0419. LUCA.
HOGENOMiHOG000280831.
InParanoidiP13458.
KOiK03546.
OMAiVWLANQQ.
PhylomeDBiP13458.

Family and domain databases

Gene3Di3.40.50.300. 4 hits.
InterProiView protein in InterPro
IPR027417. P-loop_NTPase.
IPR004592. SbcC_gammaproteobac_type.
SUPFAMiSSF52540. SSF52540. 4 hits.
TIGRFAMsiTIGR00618. sbcc. 1 hit.

Sequencei

Sequence statusi: Complete.

P13458-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKILSLRLKN LNSLKGEWKI DFTREPFASN GLFAITGPTG AGKTTLLDAI
60 70 80 90 100
CLALYHETPR LSNVSQSQND LMTRDTAECL AEVEFEVKGE AYRAFWSQNR
110 120 130 140 150
ARNQPDGNLQ VPRVELARCA DGKILADKVK DKLELTATLT GLDYGRFTRS
160 170 180 190 200
MLLSQGQFAA FLNAKPKERA ELLEELTGTE IYGQISAMVF EQHKSARTEL
210 220 230 240 250
EKLQAQASGV TLLTPEQVQS LTASLQVLTD EEKQLITAQQ QEQQSLNWLT
260 270 280 290 300
RQDELQQEAS RRQQALQQAL AEEEKAQPQL AALSLAQPAR NLRPHWERIA
310 320 330 340 350
EHSAALAHIR QQIEEVNTRL QSTMALRASI RHHAAKQSAE LQQQQQSLNT
360 370 380 390 400
WLQEHDRFRQ WNNEPAGWRA QFSQQTSDRE HLRQWQQQLT HAEQKLNALA
410 420 430 440 450
AITLTLTADE VATALAQHAE QRPLRQHLVA LHGQIVPQQK RLAQLQVAIQ
460 470 480 490 500
NVTQEQTQRN AALNEMRQRY KEKTQQLADV KTICEQEARI KTLEAQRAQL
510 520 530 540 550
QAGQPCPLCG STSHPAVEAY QALEPGVNQS RLLALENEVK KLGEEGATLR
560 570 580 590 600
GQLDAITKQL QRDENEAQSL RQDEQALTQQ WQAVTASLNI TLQPLDDIQP
610 620 630 640 650
WLDAQDEHER QLRLLSQRHE LQGQIAAHNQ QIIQYQQQIE QRQQLLLTTL
660 670 680 690 700
TGYALTLPQE DEEESWLATR QQEAQSWQQR QNELTALQNR IQQLTPILET
710 720 730 740 750
LPQSDELPHC EETVVLENWR QVHEQCLALH SQQQTLQQQD VLAAQSLQKA
760 770 780 790 800
QAQFDTALQA SVFDDQQAFL AALMDEQTLT QLEQLKQNLE NQRRQAQTLV
810 820 830 840 850
TQTAETLAQH QQHRPDDGLA LTVTVEQIQQ ELAQTHQKLR ENTTSQGEIR
860 870 880 890 900
QQLKQDADNR QQQQTLMQQI AQMTQQVEDW GYLNSLIGSK EGDKFRKFAQ
910 920 930 940 950
GLTLDNLVHL ANQQLTRLHG RYLLQRKASE ALEVEVVDTW QADAVRDTRT
960 970 980 990 1000
LSGGESFLVS LALALALSDL VSHKTRIDSL FLDEGFGTLD SETLDTALDA
1010 1020 1030 1040
LDALNASGKT IGVISHVEAM KERIPVQIKV KKINGLGYSK LESTFAVK
Length:1,048
Mass (Da):118,721
Last modified:January 1, 1990 - v1
Checksum:iBF873BC3386F98C4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15981 Genomic DNA. Translation: CAA34104.1.
U73857 Genomic DNA. Translation: AAB18121.1.
U00096 Genomic DNA. Translation: AAC73500.1.
AP009048 Genomic DNA. Translation: BAE76177.1.
M64787 Genomic DNA. Translation: AAA23473.1.
PIRiJS0350. BVECSC.
RefSeqiNP_414931.1. NC_000913.3.
WP_000698951.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73500; AAC73500; b0397.
BAE76177; BAE76177; BAE76177.
GeneIDi949076.
KEGGiecj:JW0387.
eco:b0397.
PATRICi32115937. VBIEscCol129921_0410.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15981 Genomic DNA. Translation: CAA34104.1.
U73857 Genomic DNA. Translation: AAB18121.1.
U00096 Genomic DNA. Translation: AAC73500.1.
AP009048 Genomic DNA. Translation: BAE76177.1.
M64787 Genomic DNA. Translation: AAA23473.1.
PIRiJS0350. BVECSC.
RefSeqiNP_414931.1. NC_000913.3.
WP_000698951.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP13458.
SMRiP13458.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259821. 33 interactors.
DIPiDIP-342N.
IntActiP13458. 6 interactors.
MINTiMINT-1301497.
STRINGi511145.b0397.

Proteomic databases

PaxDbiP13458.
PRIDEiP13458.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73500; AAC73500; b0397.
BAE76177; BAE76177; BAE76177.
GeneIDi949076.
KEGGiecj:JW0387.
eco:b0397.
PATRICi32115937. VBIEscCol129921_0410.

Organism-specific databases

EchoBASEiEB0920.
EcoGeneiEG10927. sbcC.

Phylogenomic databases

eggNOGiENOG4105EYH. Bacteria.
COG0419. LUCA.
HOGENOMiHOG000280831.
InParanoidiP13458.
KOiK03546.
OMAiVWLANQQ.
PhylomeDBiP13458.

Enzyme and pathway databases

BioCyciEcoCyc:EG10927-MONOMER.
MetaCyc:EG10927-MONOMER.

Miscellaneous databases

PROiP13458.

Family and domain databases

Gene3Di3.40.50.300. 4 hits.
InterProiView protein in InterPro
IPR027417. P-loop_NTPase.
IPR004592. SbcC_gammaproteobac_type.
SUPFAMiSSF52540. SSF52540. 4 hits.
TIGRFAMsiTIGR00618. sbcc. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSBCC_ECOLI
AccessioniPrimary (citable) accession number: P13458
Secondary accession number(s): Q2MC29
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: March 15, 2017
This is version 144 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.