Reviewed, UniProtKB/Swiss-Prot P13453 (TODE_PSEP1)

Names and origin

Protein names

Recommended name:
    3-methylcatechol 2,3-dioxygenase
    EC=1.13.11.-

Gene names

Name:	todE
Ordered Locus Names:	Pput_2876

Organism

Pseudomonas putida (strain F1 / ATCC 700007) [Complete proteome] [HAMAP]

Taxonomic identifier

351746 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	291 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	3-methylcatechol + O2 = 2-hydroxy-6-oxo-2,4-heptadienoate.
Cofactor	Fe2+ ion.
Pathway	Xenobiotic degradation; toluene degradation.
Subunit structure	Homooctamer.
Sequence similarities	Belongs to the extradiol ring-cleavage dioxygenase family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW xenobiotic catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	ferrous iron binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 291	290	3-methylcatechol 2,3-dioxygenase		PRO_0000085032
Sites
	Metal binding	146	1	Iron By similarity
	Metal binding	210	1	Iron By similarity
	Metal binding	260	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	P13453-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 6D5911CB1A9043FF	FASTA	291	32,209
	10 20 30 40 50 60 MSIQRLGYLG FEVADVRSWR TFATTRLGMM EASASETEAT FRIDSRAWRL SVSRGPADDY 70 80 90 100 110 120 LFAGFEVDSE QGLQEVKESL QAHGVTVKVE GGELIAKRGV LGLISCTDPF GNRVEIYYGA 130 140 150 160 170 180 TELFERPFAS PTGVSGFQTG DQGLGHYVLS VADVDAALAF YTKALGFQLA DVIDWTIGDG 190 200 210 220 230 240 LSVTLYFLYC NGRHHSFAFA KLPGSKRLHH FMLQANGMDD VGLAYDKFDA ERAVVMSLGR 250 260 270 280 290 HTNDHMISFY GATPSGFAVE YGWGAREVTR HWSVVRYDRI SIWGHKFQAP A

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Toluene degradation by Pseudomonas putida F1. Nucleotide sequence of the todC1C2BADE genes and their expression in Escherichia coli." Zylstra G.J., Gibson D.T. J. Biol. Chem. 264:14940-14946(1989) [PubMed: 2670929] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-13.
[2]	"Complete sequence of Pseudomonas putida F1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Lykidis A., Parales R., Richardson P. Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	J04996 Genomic DNA. Translation: AAA26010.1. CP000712 Genomic DNA. Translation: ABQ79007.1.
PIR	F36516.
RefSeq	YP_001268191.1.
3D structure databases
HSSP	HSSP built from PDB template 1DHY based on UniProtKB P17297.
ModBase	Search...
Genome annotation databases
GeneID	5193758.
GenomeReviews	Gene locus Pput_2876 in contig CP000712_GR.
KEGG	ppf:Pput_2876.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	P13453. RIDSRAW.
Enzyme and pathway databases
BioCyc	MetaCyc:MON-11354.
Family and domain databases
InterPro	IPR017626. DiOHbiphenyl_dOase. IPR004360. Glyas_bleo-R_dOase. IPR000486. Xdiol_dOase_1_2. [Graphical view]
Pfam	PF00903. Glyoxalase. 2 hits. [Graphical view]
ProDom	PD002334. Gly_diox. 2 hits. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR03213. 23dbph12diox. 1 hit.
PROSITE	PS00082. EXTRADIOL_DIOXYGENAS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TODE_PSEP1

Accession

Primary (citable) accession number: P13453
Secondary accession number(s): A5W4E7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 23, 2007
Last modified:	May 5, 2009
This is version 59 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)