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Protein

Nitrile hydratase subunit beta

Gene

nthB

Organism
Rhodococcus erythropolis (Arthrobacter picolinophilus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

NHase catalyzes the hydration of various nitrile compounds to the corresponding amides.

Catalytic activityi

An aliphatic amide = a nitrile + H2O.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Enzyme and pathway databases

SABIO-RKP13449.

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrile hydratase subunit beta (EC:4.2.1.84)
Short name:
NHase
Short name:
Nitrilase
Gene namesi
Name:nthB
Synonyms:nha2
OrganismiRhodococcus erythropolis (Arthrobacter picolinophilus)
Taxonomic identifieri1833 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesNocardiaceaeRhodococcus

Pathology & Biotechi

Biotechnological usei

Industrial production of acrylamide is now being developed using some of these enzymes.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001868311 – 212Nitrile hydratase subunit betaAdd BLAST212

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta chain.

Protein-protein interaction databases

DIPiDIP-6076N.
STRINGi234621.RER_59250.

Structurei

Secondary structure

1212
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni31 – 34Combined sources4
Helixi35 – 45Combined sources11
Helixi52 – 60Combined sources9
Helixi64 – 69Combined sources6
Helixi72 – 86Combined sources15
Helixi92 – 99Combined sources8
Beta strandi128 – 131Combined sources4
Helixi144 – 146Combined sources3
Beta strandi150 – 156Combined sources7
Helixi164 – 167Combined sources4
Turni168 – 170Combined sources3
Beta strandi179 – 185Combined sources7
Helixi186 – 190Combined sources5
Beta strandi197 – 204Combined sources8
Helixi205 – 207Combined sources3
Beta strandi208 – 210Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AHJX-ray2.65B/D/F/H1-212[»]
2AHJX-ray1.70B/D1-212[»]
2CYZX-ray1.55B1-212[»]
2CZ0X-ray1.50B1-212[»]
2CZ1X-ray1.39B1-212[»]
2CZ6X-ray1.50B1-212[»]
2CZ7X-ray1.80B1-212[»]
2D0QX-ray1.65B1-212[»]
2QDYX-ray1.30B1-212[»]
2ZCFX-ray1.43B1-212[»]
2ZPBX-ray1.30B1-212[»]
2ZPEX-ray1.48B1-212[»]
2ZPFX-ray1.48B1-212[»]
2ZPGX-ray1.39B1-212[»]
2ZPHX-ray1.59B1-212[»]
2ZPIX-ray1.49B1-212[»]
3A8GX-ray1.11B1-212[»]
3A8HX-ray1.66B1-212[»]
3A8LX-ray1.63B1-212[»]
3A8MX-ray1.32B1-212[»]
3A8OX-ray1.47B1-212[»]
3WVDX-ray1.18B1-212[»]
3WVEX-ray1.57B1-212[»]
3X20X-ray1.18B1-212[»]
3X24X-ray1.24B1-212[»]
3X25X-ray1.20B1-212[»]
3X26X-ray1.34B1-212[»]
3X28X-ray1.65B1-212[»]
ProteinModelPortaliP13449.
SMRiP13449.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP13449.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4108KGA. Bacteria.
ENOG410YGPC. LUCA.

Family and domain databases

InterProiIPR024690. CN_hydtase_beta_dom.
IPR008990. Elect_transpt_acc-like_dom.
IPR003168. Nitrile_hydratase_bsu.
[Graphical view]
PfamiPF02211. NHase_beta. 1 hit.
[Graphical view]
PIRSFiPIRSF001427. NHase_beta. 1 hit.
SUPFAMiSSF50090. SSF50090. 1 hit.
TIGRFAMsiTIGR03888. nitrile_beta. 1 hit.

Sequencei

Sequence statusi: Complete.

P13449-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDGVHDLAGV QGFGKVPHTV NADIGPTFHA EWEHLPYSLM FAGVAELGAF
60 70 80 90 100
SVDEVRYVVE RMEPRHYMMT PYYERYVIGV ATLMVEKGIL TQDELESLAG
110 120 130 140 150
GPFPLSRPSE SEGRPAPVET TTFEVGQRVR VRDEYVPGHI RMPAYCRGRV
160 170 180 190 200
GTISHRTTEK WPFPDAIGHG RNDAGEEPTY HVKFAAEELF GSDTDGGSVV
210
VDLFEGYLEP AA
Length:212
Mass (Da):23,487
Last modified:January 1, 1990 - v1
Checksum:iA0401CA4FC1C2CBE
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti40M → V in strain: ACV2. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54074 Genomic DNA. Translation: CAA38011.1.
X14668 Genomic DNA. Translation: CAA32798.1.
Z48769 Genomic DNA. Translation: CAA88686.1.
AB016078 Genomic DNA. Translation: BAA36598.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54074 Genomic DNA. Translation: CAA38011.1.
X14668 Genomic DNA. Translation: CAA32798.1.
Z48769 Genomic DNA. Translation: CAA88686.1.
AB016078 Genomic DNA. Translation: BAA36598.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AHJX-ray2.65B/D/F/H1-212[»]
2AHJX-ray1.70B/D1-212[»]
2CYZX-ray1.55B1-212[»]
2CZ0X-ray1.50B1-212[»]
2CZ1X-ray1.39B1-212[»]
2CZ6X-ray1.50B1-212[»]
2CZ7X-ray1.80B1-212[»]
2D0QX-ray1.65B1-212[»]
2QDYX-ray1.30B1-212[»]
2ZCFX-ray1.43B1-212[»]
2ZPBX-ray1.30B1-212[»]
2ZPEX-ray1.48B1-212[»]
2ZPFX-ray1.48B1-212[»]
2ZPGX-ray1.39B1-212[»]
2ZPHX-ray1.59B1-212[»]
2ZPIX-ray1.49B1-212[»]
3A8GX-ray1.11B1-212[»]
3A8HX-ray1.66B1-212[»]
3A8LX-ray1.63B1-212[»]
3A8MX-ray1.32B1-212[»]
3A8OX-ray1.47B1-212[»]
3WVDX-ray1.18B1-212[»]
3WVEX-ray1.57B1-212[»]
3X20X-ray1.18B1-212[»]
3X24X-ray1.24B1-212[»]
3X25X-ray1.20B1-212[»]
3X26X-ray1.34B1-212[»]
3X28X-ray1.65B1-212[»]
ProteinModelPortaliP13449.
SMRiP13449.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-6076N.
STRINGi234621.RER_59250.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4108KGA. Bacteria.
ENOG410YGPC. LUCA.

Enzyme and pathway databases

SABIO-RKP13449.

Miscellaneous databases

EvolutionaryTraceiP13449.

Family and domain databases

InterProiIPR024690. CN_hydtase_beta_dom.
IPR008990. Elect_transpt_acc-like_dom.
IPR003168. Nitrile_hydratase_bsu.
[Graphical view]
PfamiPF02211. NHase_beta. 1 hit.
[Graphical view]
PIRSFiPIRSF001427. NHase_beta. 1 hit.
SUPFAMiSSF50090. SSF50090. 1 hit.
TIGRFAMsiTIGR03888. nitrile_beta. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNHAB_RHOER
AccessioniPrimary (citable) accession number: P13449
Secondary accession number(s): Q59789
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: November 2, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.