Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

UniProtKB - P13445 (RPOS_ECOLI)

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

RNA polymerase sigma factor RpoS

Gene

rpoS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the master transcriptional regulator of the stationary phase and the general stress response. Controls, positively or negatively, the expression of several hundred genes, which are mainly involved in metabolism, transport, regulation and stress management.UniRule annotation5 Publications
Protects stationary phase cells from killing induced by endoribonuclease MazF.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi288 – 307H-T-H motifUniRule annotationAdd BLAST20

GO - Molecular functioni

  • bacterial-type RNA polymerase core enzyme binding Source: EcoCyc
  • DNA binding Source: UniProtKB-KW
  • sigma factor activity Source: EcoCyc
  • transcription factor activity, sequence-specific DNA binding Source: InterPro
Complete GO annotation...

GO - Biological processi

  • transcription initiation from bacterial-type RNA polymerase promoter Source: EcoCyc
Complete GO annotation...

Keywordsi

Molecular functionDNA-binding, Sigma factor
Biological processStress response, Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciEcoCyc:RPOS-MONOMER.
MetaCyc:RPOS-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
RNA polymerase sigma factor RpoSUniRule annotation
Alternative name(s):
Sigma SUniRule annotation
Sigma-38UniRule annotation
Gene namesi
Name:rpoSUniRule annotation
Synonyms:appR, katF, nur, otsX, sigS
Ordered Locus Names:b2741, JW5437
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia ›
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10510. rpoS.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

  • cytoplasm Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Stationary phase cells lose resistance to killing by endoribonuclease MazF.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi173K → E: Eliminates RpoS proteolysis. Lack of interaction with RssB. 1 Publication1
Mutagenesisi174E → T: 2-fold increase in RpoS half-life. Does not affect interaction with RssB. 1 Publication1
Mutagenesisi177V → K: 3-fold increase in RpoS half-life. 1 Publication1
Mutagenesisi178Y → L: Does not affect RpoS half-life. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000939701 – 330RNA polymerase sigma factor RpoSAdd BLAST330

Proteomic databases

PaxDbiP13445.
PRIDEiP13445.

Expressioni

Inductioni

Subject to complex regulation at multiple levels (transcription, translation, regulation of activity and degradation). Transcription is induced during entry into stationary phase and in response to different stresses. Transcription is repressed by antitoxin MqsA (PubMed:21516113). mRNA stability is regulated by small RNA regulators and various proteins such as Hfq, CsdA, CspC and CspE. Finally, the cellular level of RpoS is regulated by proteolysis via RssB and the ClpXP protease.6 Publications

Interactioni

Subunit structurei

Interacts with the RNA polymerase core enzyme and RssB.UniRule annotation2 Publications

GO - Molecular functioni

  • bacterial-type RNA polymerase core enzyme binding Source: EcoCyc
Complete GO annotation...

Protein-protein interaction databases

BioGridi4262274. 303 interactors.
DIPiDIP-10777N.
IntActiP13445. 9 interactors.
MINTiMINT-1228474.
STRINGi316385.ECDH10B_2909.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5IPLX-ray3.60F1-328[»]
5IPMX-ray4.20F1-328[»]
5IPNX-ray4.61F1-328[»]
ProteinModelPortaliP13445.
SMRiP13445.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni56 – 89Sigma-70 factor domain-1Add BLAST34
Regioni94 – 164Sigma-70 factor domain-2Add BLAST71
Regioni174 – 249Sigma-70 factor domain-3Add BLAST76
Regioni262 – 315Sigma-70 factor domain-4Add BLAST54

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi118 – 121Interaction with polymerase core subunit RpoC4

Domaini

The sigma-70 factor domain-2 mediates sequence-specific interaction with the -10 element in promoter DNA, and plays an important role in melting the double-stranded DNA and the formation of the transcription bubble. The sigma-70 factor domain-2 mediates interaction with the RNA polymerase subunits RpoB and RpoC (By similarity).By similarity
The sigma-70 factor domain-4 contains a helix-turn-helix (H-T-H) motif that mediates interaction with the -35 element in promoter DNA. The domain also mediates interaction with the RNA polymerase subunit RpoA (By similarity).By similarity

Sequence similaritiesi

Belongs to the sigma-70 factor family. RpoS subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DG1. Bacteria.
COG0568. LUCA.
HOGENOMiHOG000270273.
InParanoidiP13445.
KOiK03087.
PhylomeDBiP13445.

Family and domain databases

Gene3Di1.10.10.10. 2 hits.
HAMAPiMF_00959. Sigma70_RpoS. 1 hit.
InterProiView protein in InterPro
IPR014284. RNA_pol_sigma-70_dom.
IPR000943. RNA_pol_sigma70.
IPR009042. RNA_pol_sigma70_r1_2.
IPR007627. RNA_pol_sigma70_r2.
IPR007624. RNA_pol_sigma70_r3.
IPR007630. RNA_pol_sigma70_r4.
IPR013325. RNA_pol_sigma_r2.
IPR013324. RNA_pol_sigma_r3_r4.
IPR012761. RNA_pol_sigma_RpoS.
IPR011991. WHTH_DNA-bd_dom.
PfamiView protein in Pfam
PF00140. Sigma70_r1_2. 1 hit.
PF04542. Sigma70_r2. 1 hit.
PF04539. Sigma70_r3. 1 hit.
PF04545. Sigma70_r4. 1 hit.
PRINTSiPR00046. SIGMA70FCT.
SUPFAMiSSF88659. SSF88659. 2 hits.
SSF88946. SSF88946. 1 hit.
TIGRFAMsiTIGR02394. rpoS_proteo. 1 hit.
TIGR02937. sigma70-ECF. 1 hit.
PROSITEiView protein in PROSITE
PS00715. SIGMA70_1. 1 hit.
PS00716. SIGMA70_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P13445-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQNTLKVHD LNEDAEFDEN GVEVFDEKAL VEQEPSDNDL AEEELLSQGA 
        60         70         80         90        100
TQRVLDATQL YLGEIGYSPL LTAEEEVYFA RRALRGDVAS RRRMIESNLR 
       110        120        130        140        150
LVVKIARRYG NRGLALLDLI EEGNLGLIRA VEKFDPERGF RFSTYATWWI 
       160        170        180        190        200
RQTIERAIMN QTRTIRLPIH IVKELNVYLR TARELSHKLD HEPSAEEIAE 
       210        220        230        240        250
QLDKPVDDVS RMLRLNERIT SVDTPLGGDS EKALLDILAD EKENGPEDTT 
       260        270        280        290        300
QDDDMKQSIV KWLFELNAKQ REVLARRFGL LGYEAATLED VGREIGLTRE 
       310        320        330
RVRQIQVEGL RRLREILQTQ GLNIEALFRE
Length:330
Mass (Da):37,972
Last modified:October 1, 1996 - v3
Checksum:i4E6E675E4CA6BC6F
GO

Sequence cautioni

The sequence BAE76818 differs from that shown. Reason: Erroneous termination at position 33. Translated as Gln.Curated
The sequence CAA34435 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence CAA78692 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti25F → L in CAA78692 (PubMed:1437569).Curated1
Sequence conflicti29A → P in CAA34435 (PubMed:2690013).Curated1
Sequence conflicti33Q → L in BAA02747 (PubMed:8475100).Curated1
Sequence conflicti33Q → L in BAA21003 (PubMed:8208244).Curated1
Sequence conflicti196E → V in CAA34435 (PubMed:2690013).Curated1
Sequence conflicti328 – 330FRE → LPRVSKHLSERPVSSEAGFF CAQ in CAA34435 (PubMed:2690013).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X16400 Genomic DNA. Translation: CAA34435.1. Different initiation.
D13548 Genomic DNA. Translation: BAA02747.1.
Z14969 Genomic DNA. Translation: CAA78692.1. Different initiation.
U29579 Genomic DNA. Translation: AAA69251.1.
U00096 Genomic DNA. Translation: AAC75783.1.
AP009048 Genomic DNA. Translation: BAE76818.1. Sequence problems.
D17549 Genomic DNA. Translation: BAA21003.1.
L07869 Unassigned DNA. Translation: AAA17876.1.
PIRiA65055. RNECKF.
RefSeqiNP_417221.1. NC_000913.3.
WP_000081588.1. NZ_CP014272.1.

Genome annotation databases

EnsemblBacteriaiAAC75783; AAC75783; b2741.
BAE76818; BAE76818; BAE76818.
GeneIDi947210.
KEGGiecj:JW5437.
eco:b2741.
PATRICifig|511145.12.peg.2835.

Similar proteinsi

Entry informationi

Entry nameiRPOS_ECOLI
AccessioniPrimary (citable) accession number: P13445
Secondary accession number(s): Q2MA88, Q79EB7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: October 1, 1996
Last modified: August 30, 2017
This is version 161 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families