UniProtKB - P13445 (RPOS_ECOLI)
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Protein
RNA polymerase sigma factor RpoS
Gene
rpoS
Organism
Escherichia coli (strain K12)
Status
Functioni
Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the master transcriptional regulator of the stationary phase and the general stress response. Controls, positively or negatively, the expression of several hundred genes, which are mainly involved in metabolism, transport, regulation and stress management.UniRule annotation5 Publications
Protects stationary phase cells from killing induced by endoribonuclease MazF.1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 288 – 307 | H-T-H motifUniRule annotationAdd BLAST | 20 |
GO - Molecular functioni
- bacterial sigma factor activity Source: EcoCyc
- bacterial-type RNA polymerase core enzyme binding Source: EcoCyc
- DNA binding Source: UniProtKB-KW
- DNA binding transcription factor activity Source: InterPro
GO - Biological processi
Keywordsi
Molecular function | DNA-binding, Sigma factor |
Biological process | Stress response, Transcription, Transcription regulation |
Enzyme and pathway databases
BioCyci | EcoCyc:RPOS-MONOMER. MetaCyc:RPOS-MONOMER. |
Names & Taxonomyi
Protein namesi | Recommended name: RNA polymerase sigma factor RpoSUniRule annotationAlternative name(s): Sigma SUniRule annotation Sigma-38UniRule annotation |
Gene namesi | Ordered Locus Names:b2741, JW5437 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Organism-specific databases
EcoGenei | EG10510. rpoS. |
Subcellular locationi
- Cytoplasm UniRule annotation
GO - Cellular componenti
- cytoplasm Source: EcoCyc
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
Stationary phase cells lose resistance to killing by endoribonuclease MazF.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 173 | K → E: Eliminates RpoS proteolysis. Lack of interaction with RssB. 1 Publication | 1 | |
Mutagenesisi | 174 | E → T: 2-fold increase in RpoS half-life. Does not affect interaction with RssB. 1 Publication | 1 | |
Mutagenesisi | 177 | V → K: 3-fold increase in RpoS half-life. 1 Publication | 1 | |
Mutagenesisi | 178 | Y → L: Does not affect RpoS half-life. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000093970 | 1 – 330 | RNA polymerase sigma factor RpoSAdd BLAST | 330 |
Proteomic databases
EPDi | P13445. |
PaxDbi | P13445. |
PRIDEi | P13445. |
Expressioni
Inductioni
Subject to complex regulation at multiple levels (transcription, translation, regulation of activity and degradation). Transcription is induced during entry into stationary phase and in response to different stresses. Transcription is repressed by antitoxin MqsA (PubMed:21516113). mRNA stability is regulated by small RNA regulators and various proteins such as Hfq, CsdA, CspC and CspE. Finally, the cellular level of RpoS is regulated by proteolysis via RssB and the ClpXP protease.6 Publications
Interactioni
Subunit structurei
Interacts with the RNA polymerase core enzyme and RssB.UniRule annotation2 Publications
Binary interactionsi
With | Entry | #Exp. | IntAct | Notes |
---|---|---|---|---|
rpoB | P0A8V2 | 5 | EBI-557581,EBI-544996 |
GO - Molecular functioni
- bacterial-type RNA polymerase core enzyme binding Source: EcoCyc
Protein-protein interaction databases
BioGridi | 4262274. 308 interactors. |
DIPi | DIP-10777N. |
IntActi | P13445. 9 interactors. |
STRINGi | 316385.ECDH10B_2909. |
Structurei
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5IPL | X-ray | 3.60 | F | 1-328 | [»] | |
5IPM | X-ray | 4.20 | F | 1-328 | [»] | |
5IPN | X-ray | 4.61 | F | 1-328 | [»] | |
ProteinModelPortali | P13445. | |||||
SMRi | P13445. | |||||
ModBasei | Search... | |||||
MobiDBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 56 – 89 | Sigma-70 factor domain-1Add BLAST | 34 | |
Regioni | 94 – 164 | Sigma-70 factor domain-2Add BLAST | 71 | |
Regioni | 174 – 249 | Sigma-70 factor domain-3Add BLAST | 76 | |
Regioni | 262 – 315 | Sigma-70 factor domain-4Add BLAST | 54 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 118 – 121 | Interaction with polymerase core subunit RpoC | 4 |
Domaini
The sigma-70 factor domain-2 mediates sequence-specific interaction with the -10 element in promoter DNA, and plays an important role in melting the double-stranded DNA and the formation of the transcription bubble. The sigma-70 factor domain-2 mediates interaction with the RNA polymerase subunits RpoB and RpoC (By similarity).By similarity
The sigma-70 factor domain-4 contains a helix-turn-helix (H-T-H) motif that mediates interaction with the -35 element in promoter DNA. The domain also mediates interaction with the RNA polymerase subunit RpoA (By similarity).By similarity
Sequence similaritiesi
Phylogenomic databases
eggNOGi | ENOG4105DG1. Bacteria. COG0568. LUCA. |
HOGENOMi | HOG000270273. |
InParanoidi | P13445. |
KOi | K03087. |
OMAi | ITNRESQ. |
PhylomeDBi | P13445. |
Family and domain databases
Gene3Di | 1.10.10.10. 2 hits. |
HAMAPi | MF_00959. Sigma70_RpoS. 1 hit. |
InterProi | View protein in InterPro IPR014284. RNA_pol_sigma-70_dom. IPR000943. RNA_pol_sigma70. IPR009042. RNA_pol_sigma70_r1_2. IPR007627. RNA_pol_sigma70_r2. IPR007624. RNA_pol_sigma70_r3. IPR007630. RNA_pol_sigma70_r4. IPR013325. RNA_pol_sigma_r2. IPR013324. RNA_pol_sigma_r3_r4. IPR012761. RNA_pol_sigma_RpoS. IPR036388. WH-like_DNA-bd_sf. |
Pfami | View protein in Pfam PF00140. Sigma70_r1_2. 1 hit. PF04542. Sigma70_r2. 1 hit. PF04539. Sigma70_r3. 1 hit. PF04545. Sigma70_r4. 1 hit. |
PRINTSi | PR00046. SIGMA70FCT. |
SUPFAMi | SSF88659. SSF88659. 2 hits. SSF88946. SSF88946. 1 hit. |
TIGRFAMsi | TIGR02394. rpoS_proteo. 1 hit. TIGR02937. sigma70-ECF. 1 hit. |
PROSITEi | View protein in PROSITE PS00715. SIGMA70_1. 1 hit. PS00716. SIGMA70_2. 1 hit. |
i Sequence
Sequence statusi: Complete.
P13445-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSQNTLKVHD LNEDAEFDEN GVEVFDEKAL VEQEPSDNDL AEEELLSQGA
60 70 80 90 100
TQRVLDATQL YLGEIGYSPL LTAEEEVYFA RRALRGDVAS RRRMIESNLR
110 120 130 140 150
LVVKIARRYG NRGLALLDLI EEGNLGLIRA VEKFDPERGF RFSTYATWWI
160 170 180 190 200
RQTIERAIMN QTRTIRLPIH IVKELNVYLR TARELSHKLD HEPSAEEIAE
210 220 230 240 250
QLDKPVDDVS RMLRLNERIT SVDTPLGGDS EKALLDILAD EKENGPEDTT
260 270 280 290 300
QDDDMKQSIV KWLFELNAKQ REVLARRFGL LGYEAATLED VGREIGLTRE
310 320 330
RVRQIQVEGL RRLREILQTQ GLNIEALFRE
Sequence cautioni
The sequence BAE76818 differs from that shown. Reason: Erroneous termination at position 33. Translated as Gln.Curated
The sequence CAA34435 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence CAA78692 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 25 | F → L in CAA78692 (PubMed:1437569).Curated | 1 | |
Sequence conflicti | 29 | A → P in CAA34435 (PubMed:2690013).Curated | 1 | |
Sequence conflicti | 33 | Q → L in BAA02747 (PubMed:8475100).Curated | 1 | |
Sequence conflicti | 33 | Q → L in BAA21003 (PubMed:8208244).Curated | 1 | |
Sequence conflicti | 196 | E → V in CAA34435 (PubMed:2690013).Curated | 1 | |
Sequence conflicti | 328 – 330 | FRE → LPRVSKHLSERPVSSEAGFF CAQ in CAA34435 (PubMed:2690013).Curated | 3 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X16400 Genomic DNA. Translation: CAA34435.1. Different initiation. D13548 Genomic DNA. Translation: BAA02747.1. Z14969 Genomic DNA. Translation: CAA78692.1. Different initiation. U29579 Genomic DNA. Translation: AAA69251.1. U00096 Genomic DNA. Translation: AAC75783.1. AP009048 Genomic DNA. Translation: BAE76818.1. Sequence problems. D17549 Genomic DNA. Translation: BAA21003.1. L07869 Unassigned DNA. Translation: AAA17876.1. |
PIRi | A65055. RNECKF. |
RefSeqi | NP_417221.1. NC_000913.3. WP_000081586.1. NZ_CP011343.2. WP_000081588.1. NZ_CP014272.1. |
Genome annotation databases
EnsemblBacteriai | AAC75783; AAC75783; b2741. BAE76818; BAE76818; BAE76818. |
GeneIDi | 947210. |
KEGGi | ecj:JW5437. eco:b2741. |
PATRICi | fig|511145.12.peg.2835. |
Similar proteinsi
Entry informationi
Entry namei | RPOS_ECOLI | |
Accessioni | P13445Primary (citable) accession number: P13445 Secondary accession number(s): Q2MA88, Q79EB7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1990 |
Last sequence update: | October 1, 1996 | |
Last modified: | March 28, 2018 | |
This is version 166 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |