P13445 (RPOS_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA polymerase sigma factor RpoS

Alternative name(s):
Sigma-38

Gene names

Name:	rpoS
Synonyms:	appR, katF, nur, otsX, sigS
Ordered Locus Names:	b2741, JW5437

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Sigma transcription factor controlling a regulon of genes required for protection against external stresses. Possibly, it affects the expression of katE (catalase HPII), xthA (endonuclease III), the appABC operon and other genes by modifying the affinity of RNA polymerase for the promoters.
Sequence similarities	Belongs to the sigma-70 factor family.
Sequence caution	The sequence BAE76818.1 differs from that shown. Reason: Erroneous termination at position 33. Translated as Gln. The sequence CAA34435.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA78692.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Sigma factor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	response to stress Inferred from direct assay PubMed 21398637. Source: EcoCyc transcription initiation from bacterial-type RNA polymerase promoter Inferred from direct assay PubMed 21398637. Source: EcoCyc
Cellular_component	cytoplasm Inferred from direct assay PubMed 15629914. Source: EcoCyc
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW sequence-specific DNA binding transcription factor activity Inferred from direct assay PubMed 11737638. Source: EcoCyc sigma factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 330	330	RNA polymerase sigma factor RpoS		PRO_0000093970
Regions
	DNA binding	288 – 307	20	H-T-H motif By similarity
	Motif	118 – 131	14	Polymerase core binding Potential
Experimental info
	Sequence conflict	25	1	F → L in CAA78692. Ref.3
	Sequence conflict	29	1	A → P in CAA34435. Ref.1
	Sequence conflict	33	1	Q → L Ref.2
	Sequence conflict	33	1	Q → L Ref.6
	Sequence conflict	196	1	E → V in CAA34435. Ref.1
	Sequence conflict	328 – 330	3	FRE → LPRVSKHLSERPVSSEAGFF CAQ Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P13445 [UniParc]. Last modified October 1, 1996. Version 3. Checksum: 4E6E675E4CA6BC6F	FASTA	330	37,972
	10 20 30 40 50 60 MSQNTLKVHD LNEDAEFDEN GVEVFDEKAL VEQEPSDNDL AEEELLSQGA TQRVLDATQL 70 80 90 100 110 120 YLGEIGYSPL LTAEEEVYFA RRALRGDVAS RRRMIESNLR LVVKIARRYG NRGLALLDLI 130 140 150 160 170 180 EEGNLGLIRA VEKFDPERGF RFSTYATWWI RQTIERAIMN QTRTIRLPIH IVKELNVYLR 190 200 210 220 230 240 TARELSHKLD HEPSAEEIAE QLDKPVDDVS RMLRLNERIT SVDTPLGGDS EKALLDILAD 250 260 270 280 290 300 EKENGPEDTT QDDDMKQSIV KWLFELNAKQ REVLARRFGL LGYEAATLED VGREIGLTRE 310 320 330 RVRQIQVEGL RRLREILQTQ GLNIEALFRE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of katF of Escherichia coli suggests KatF protein is a novel sigma transcription factor." Mulvey M.R., Loewen P.C. Nucleic Acids Res. 17:9979-9991(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Heterogeneity of the principal sigma factor in Escherichia coli: the rpoS gene product, sigma 38, is a second principal sigma factor of RNA polymerase in stationary-phase Escherichia coli." Tanaka K., Takayanagi Y., Fujita N., Ishihama A., Takahashi H. Proc. Natl. Acad. Sci. U.S.A. 90:3511-3515(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"DNA base sequence variability in katF (putative sigma factor) gene of Escherichia coli." Ivanova A., Renshaw M., Guntaka R.V., Eisenstark A. Nucleic Acids Res. 20:5479-5480(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Structure of the 5' upstream region and the regulation of the rpoS gene of Escherichia coli." Takayanagi Y., Tanaka K., Takahashi H. Mol. Gen. Genet. 243:525-531(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-72. Strain: K12 / DH1 / ATCC 33849 / DSM 4235 / NCIB 12045.
[7]	"A gene at 59 minutes on the Escherichia coli chromosome encodes a lipoprotein with unusual amino acid repeat sequences." Ichikawa J.K., Li C., Fu J.C., Clarke S. J. Bacteriol. 176:1630-1638(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-11. Strain: MP180.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X16400 Genomic DNA. Translation: CAA34435.1. Different initiation. D13548 Genomic DNA. Translation: BAA02747.1. Z14969 Genomic DNA. Translation: CAA78692.1. Different initiation. U29579 Genomic DNA. Translation: AAA69251.1. U00096 Genomic DNA. Translation: AAC75783.1. AP009048 Genomic DNA. Translation: BAE76818.1. Sequence problems. D17549 Genomic DNA. Translation: BAA21003.1. L07869 Unassigned DNA. Translation: AAA17876.1.
PIR	RNECKF. A65055.
RefSeq	NP_417221.1. NC_000913.2. YP_490950.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P13445.
SMR	P13445. Positions 89-314.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-10777N.
IntAct	P13445. 7 interactions.
MINT	MINT-1228474.
STRING	511145.b2741.
Proteomic databases
PaxDb	P13445.
PRIDE	P13445.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC75783; AAC75783; b2741. BAE76818; BAE76818; BAE76818.
GeneID	12933280. 947210.
KEGG	ecj:Y75_p2679. eco:b2741.
PATRIC	32120886. VBIEscCol129921_2835.
Organism-specific databases
EchoBASE	EB0505.
EcoGene	EG10510. rpoS.
Phylogenomic databases
eggNOG	COG0568.
HOGENOM	HOG000270273.
KO	K03087.
OMA	AARQVMI.
ProtClustDB	PRK05657.
Enzyme and pathway databases
BioCyc	EcoCyc:RPOS-MONOMER. ECOL316407:JW5437-MONOMER. MetaCyc:RPOS-MONOMER.
Gene expression databases
Genevestigator	P13445.
Family and domain databases
Gene3D	1.10.10.10. 2 hits.
InterPro	IPR014284. RNA_pol_sigma-70_dom. IPR000943. RNA_pol_sigma70. IPR009042. RNA_pol_sigma70_r1_2. IPR007627. RNA_pol_sigma70_r2. IPR007624. RNA_pol_sigma70_r3. IPR007630. RNA_pol_sigma70_r4. IPR013325. RNA_pol_sigma_r2. IPR013324. RNA_pol_sigma_r3_r4. IPR012761. RNA_pol_sigma_RpoS_proteobac. IPR011991. WHTH_DNA-bd_dom. [Graphical view]
Pfam	PF00140. Sigma70_r1_2. 1 hit. PF04542. Sigma70_r2. 1 hit. PF04539. Sigma70_r3. 1 hit. PF04545. Sigma70_r4. 1 hit. [Graphical view]
PRINTS	PR00046. SIGMA70FCT.
SUPFAM	SSF88946. Sigma_r2. 1 hit. SSF88659. Sigma_r3_r4. 2 hits.
TIGRFAMs	TIGR02394. rpoS_proteo. 1 hit. TIGR02937. sigma70-ECF. 1 hit.
PROSITE	PS00715. SIGMA70_1. 1 hit. PS00716. SIGMA70_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPOS_ECOLI

Accession

Primary (citable) accession number: P13445
Secondary accession number(s): Q2MA88, Q79EB7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 130 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program