Reviewed, UniProtKB/Swiss-Prot P13443 (DHGY_CUCSA)

Names and origin

Protein names

Recommended name:
    Glycerate dehydrogenase
      Short name=GDH
    EC=1.1.1.29
Alternative name(s):
    NADH-dependent hydroxypyruvate reductase
      Short name=HPR

Gene names

Name:

HPR-A

Organism

Cucumis sativus (Cucumber)

Taxonomic identifier

3659 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Cucurbitales › Cucurbitaceae › Cucumis

Protein attributes

Sequence length	382 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	(R)-glycerate + NAD+ = hydroxypyruvate + NADH.
Pathway	Photosynthesis; photorespiration; 3-phospho-D-glycerate from glycine: step 3/4.
Subcellular location	Peroxisome.
Induction	The appearance of HPR in the cotyledons of cucumber seedlings is both developmentally and light-regulated possibly at the level of transcription.
Sequence similarities	Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.

Ontologies

Keywords
Biological process	Glycolate pathway Photorespiration
Cellular component	Peroxisome
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW oxidative photosynthetic carbon pathway Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	peroxisome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro glycerate dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 382	382	Glycerate dehydrogenase		PRO_0000075941
Regions
	Nucleotide binding	175 – 176	2	NAD By similarity
	Nucleotide binding	271 – 273	3	NAD By similarity
	Nucleotide binding	320 – 323	4	NAD By similarity
Sites
	Active site	273	1	By similarity
	Active site	302	1	By similarity
	Active site	320	1	Proton donor By similarity
	Binding site	297	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	P13443-1 [UniParc]. Last modified January 1, 1990. Version 1. Checksum: 4E326485ACD0E080	FASTA	382	41,706
	10 20 30 40 50 60 MAKPVQIEVW NPNGKYRVVS TKPMPGTRWI NLLIEQDCRV EICTEKKTIL SVEDILALIG 70 80 90 100 110 120 DKCDGVIGQL TEDWGEVLFS ALSRAGGKAF SNMAVGYNNV DVNAANKYGV AVGNTPGVLT 130 140 150 160 170 180 ETTAELAASL SLAAARRIVE ADEFMRAGRY DGWLPNLFVG NLLKGQTVGV IGAGRIGSAY 190 200 210 220 230 240 ARMMVEGFKM NLIYFDLYQS TRLEKFVTAY GEFLKANGEA PVTWRRASSM DEVLREADVI 250 260 270 280 290 300 SLHPVLDKTT FHLVNKESLK AMKKDAILIN CSRGPVIDEA ALVDHLRDNP MFRVGLDVFE 310 320 330 340 350 360 DEPYMKPGLA DMKNAIIVPH IASASKWTRE GMATLAALNV LGKIKGYPVW SDPNRVEPFL 370 380 DENVSPPAAS PSIVNAKALG NA

References

[1]	"Isolation, characterization and sequence analysis of a full-length cDNA clone encoding NADH-dependent hydroxypyruvate reductase from cucumber." Greenler J.M., Sloan J.S., Schwartz B.W., Becker W.M. Plant Mol. Biol. 13:139-150(1989) [PubMed: 2519111] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Improved long green. Tissue: Cotyledon.
[2]	"Characterization of genes encoding hydroxypyruvate reductase in cucumber." Schwartz B.W., Sloan J.S., Becker W.M. Plant Mol. Biol. 17:941-947(1991) [PubMed: 1912510] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Wisconsin 2238G.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X14609 mRNA. Translation: CAA32764.1. X58542 Genomic DNA. Translation: CAA41434.1.
PIR	DEKVG. S17733.
3D structure databases
HSSP	HSSP built from PDB template 2DLD based on UniProtKB P30901.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.1.1.29. 1241.
Family and domain databases
InterPro	IPR006139. D-isomer_2_OHA_DH. IPR006140. D-isomer_2_OHA_DH_NAD-bd. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00389. 2-Hacid_dh. 1 hit. PF02826. 2-Hacid_dh_C. 1 hit. [Graphical view]
PROSITE	PS00065. D_2_HYDROXYACID_DH_1. 1 hit. PS00670. D_2_HYDROXYACID_DH_2. False negative. PS00671. D_2_HYDROXYACID_DH_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHGY_CUCSA

Accession

Primary (citable) accession number: P13443

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 1, 1990
Last modified:	September 22, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)