Reviewed, UniProtKB/Swiss-Prot P13439 (PYR5_MOUSE)

Names and origin

Protein names

Recommended name:
    Uridine 5'-monophosphate synthase
      Short name=UMP synthase
Including the following 2 domains:
    1- Recommended name:
            Orotate phosphoribosyltransferase
                Short name=OPRTase
              EC=2.4.2.10
    2- Recommended name:
            Orotidine 5'-phosphate decarboxylase
              EC=4.1.1.23
        Alternative name(s):
            OMPdecase

Gene names

Name:

Umps

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	481 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate. Orotidine 5'-phosphate = UMP + CO2.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2. Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 2/2.
Subunit structure	Homodimer By similarity.
Sequence similarities	In the N-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family. In the C-terminal section; belongs to the OMP decarboxylase family.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Molecular function	Decarboxylase Glycosyltransferase Lyase Transferase
PTM	Acetylation Phosphoprotein
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro nucleoside metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	orotate phosphoribosyltransferase activity Inferred from electronic annotation. Source: EC orotidine-5'-phosphate decarboxylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 481	481	Uridine 5'-monophosphate synthase		PRO_0000139650
Regions
	Region	1 – 214	214	OPRTase
	Region	215 – 220	6	Domain linker
	Region	221 – 481	261	OMPdecase
Sites
	Active site	314	1	By similarity
	Active site	314	1	For OMPdecase activity By similarity
	Active site	317	1	For OMPdecase activity By similarity
Amino acid modifications
	Modified residue	37	1	Phosphotyrosine By similarity
	Modified residue	162	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P13439-1 [UniParc]. Last modified June 6, 2002. Version 3. Checksum: 761C7EF7960C9C59	FASTA	481	52,292
	10 20 30 40 50 60 MEVASQALGP LVTELYDVQA FKFGSFVLKS GLSSPVYIDL RGIVSRPRLL SQVADILFQT 70 80 90 100 110 120 AKNAGISFDS VCGVPYTALP LATVICSANH IPMLIRRKET KDYGTKRLVE GEINPGQTCL 130 140 150 160 170 180 VIEDVVTSGA SVLETVEVLQ KEGLKVTDAI VLLDREQGGK DKLQAQGIRL HAVCTLSQML 190 200 210 220 230 240 EILQQQEKID ADMVGRVKRF IQENVFSAAN HNGLPPPEKK ACKELSFGAR AELPGTHPLA 250 260 270 280 290 300 SKLLRLMQKK ETNLCLSADV SEARELLQLA DALGPSICML KTHVDILNDF TLDVMEELTA 310 320 330 340 350 360 LAKRHEFLIF EDRKFADIGN TVKKQYESGT FKIASWADIV NAHVVPGSGV VKGLQEVGLP 370 380 390 400 410 420 LHRACLLIAE MSSAGSLATG NYTKAAVGMA EEHCEFVIGF ISGSRVSMKP EFLHLTPGVQ 430 440 450 460 470 480 LETGGDHLGQ QYNSPQEVIG KRGSDVIIVG RGILAAANRL EAAEMYRKAA WEAYLSRLAV Q

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]	"Expression and sequence analysis of a cDNA encoding the orotidine-5'-monophosphate decarboxylase domain from Ehrlich ascites uridylate synthase." Ohmstede C.A., Langdon S.D., Chae C.B., Jones M.E. J. Biol. Chem. 261:4276-4282(1986) [PubMed: 2419341] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 216-481.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC003887 mRNA. Translation: AAH03887.1. M29395 mRNA. Translation: AAA39859.1.
IPI	IPI00121552.
PIR	DCMSOP. A25323.
RefSeq	NP_033497.1.
UniGene	Mm.13145 Mm.466951
3D structure databases
HSSP	HSSP built from PDB template 1DQW based on UniProtKB P03962.
SMR	P13439. Positions 221-479.
ModBase	Search...
Protein-protein interaction databases
STRING	P13439.
PTM databases
PhosphoSite	P13439.
Proteomic databases
PRIDE	P13439.
Genome annotation databases
Ensembl	ENSMUST00000023510; ENSMUSP00000023510; ENSMUSG00000022814; Mus musculus. [Genome view]
GeneID	22247.
KEGG	mmu:22247.
UCSC	uc007zap.1. mouse.
Organism-specific databases
CTD	22247.
MGI	MGI:1298388. Umps.
Phylogenomic databases
HOGENOM	P13439.
HOVERGEN	P13439.
OMA	PLATVIC.
Enzyme and pathway databases
BRENDA	2.4.2.10. 244. 4.1.1.23. 244.
Gene expression databases
ArrayExpress	P13439.
Bgee	P13439.
CleanEx	MM_UMPS.
Genevestigator	P13439.
GermOnline	ENSMUSG00000022814. Mus musculus.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR014732. OMPdecase_1_core. IPR018089. OMPdecase_AS. IPR001754. OMPdecase_core. IPR004467. Or_phspho_trans. IPR002375. Pr/py_Pribosyl_transf_CS. IPR000836. PRibTrfase. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF00215. OMPdecase. 1 hit. PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR00336. pyrE. 1 hit. TIGR01740. pyrF. 1 hit.
PROSITE	PS00156. OMPDECASE. 1 hit. PS00103. PUR_PYR_PR_TRANSFER. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	302319.
SOURCE	Search...

Entry information

Entry name

PYR5_MOUSE

Accession

Primary (citable) accession number: P13439
Secondary accession number(s): Q99L26

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	June 6, 2002
Last modified:	November 3, 2009
This is version 88 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)