P13434 (HAP3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcriptional activator HAP3

Alternative name(s):
UAS2 regulatory protein A

Gene names

Name:	HAP3
Ordered Locus Names:	YBL021C
ORF Names:	YBL0441

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	144 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts a component of the CCAT-binding factor, which is a transcriptional activator and binds to the upstream activation site (UAS2) of the CYC1 gene and other genes involved in mitochondrial electron transport and activates their expression. Recognizes the sequence 5'-CCAAT-3'.
Subunit structure	Component of the CCAT-binding factor (CBF or HAP complex II), which consists of one copy each of HAP2, HAP3, HAP4 and HAP5. The assembly of the HAP2-HAP3-HAP5 heteromer (HAP complex I) occurs in a one-step pathway and its binding to DNA is a prerequisite for the association of HAP4.
Subcellular location	Nucleus.
Miscellaneous	Present with 2510 molecules/cell in log phase SD medium. Ref.8
Sequence similarities	Belongs to the NFYB/HAP3 subunit family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Activator
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	carbon catabolite activation of transcription from RNA polymerase II promoter Inferred from direct assay. Source: SGD regulation of cellular respiration Inferred from mutant phenotype. Source: SGD transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	CCAAT-binding factor complex Inferred from direct assay. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
HAP2	P06774	4	EBI-8157,EBI-8152
HAP5	Q02516	5	EBI-8157,EBI-8165

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 144	144	Transcriptional activator HAP3		PRO_0000204630
Regions
	DNA binding	42 – 48	7
	Region	69 – 80	12	Subunit association domain (SAD)

Sequences

	Sequence		Length	Mass (Da)
	P13434 [UniParc]. Last modified January 1, 1990. Version 1. Checksum: 7AB5027BAE420337	FASTA	144	16,154
	10 20 30 40 50 60 MNTNESEHVS TSPEDTQENG GNASSSGSLQ QISTLREQDR WLPINNVARL MKNTLPPSAK 70 80 90 100 110 120 VSKDAKECMQ ECVSELISFV TSEASDRCAA DKRKTINGED ILISLHALGF ENYAEVLKIY 130 140 LAKYRQQQAL KNQLMYEQDD EEVP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The HAP3 regulatory locus of Saccharomyces cerevisiae encodes divergent overlapping transcripts." Hahn S., Pinkham J., Wei R., Miller R., Guarente L. Mol. Cell. Biol. 8:655-663(1988) [PubMed: 2832732] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC MYA-3516 / BWG1-7A.
[2]	"PIM1 encodes a mitochondrial ATP-dependent protease that is required for mitochondrial function in the yeast Saccharomyces cerevisiae." van Dyck L., Pearce D.A., Sherman F. J. Biol. Chem. 269:238-242(1994) [PubMed: 8276800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	"Mutations in yeast HAP2/HAP3 define a hybrid CCAAT box binding domain." Xing Y., Fikes J.D., Guarente L. EMBO J. 12:4647-4655(1993) [PubMed: 8223474] [Abstract] Cited for: MUTAGENESIS.
[7]	"A multiprotein complex that interacts with RNA polymerase II elongator." Li Y., Takagi Y., Jiang Y., Tokunaga M., Erdjument-Bromage H., Tempst P., Kornberg R.D. J. Biol. Chem. 276:29628-29631(2001) [PubMed: 11390369] [Abstract] Cited for: IDENTIFICATION IN THE CCAT-BINDING FACTOR.
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"Assembly of the Hap2p/Hap3p/Hap4p/Hap5p-DNA complex in Saccharomyces cerevisiae." McNabb D.S., Pinto I. Eukaryot. Cell 4:1829-1839(2005) [PubMed: 16278450] [Abstract] Cited for: ASSEMBLY OF THE CCAT-BINDING FACTOR.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M20318 Genomic DNA. Translation: AAA53538.1. X74544 Genomic DNA. Translation: CAA52633.1. Z35782 Genomic DNA. Translation: CAA84840.1. AY558459 Genomic DNA. Translation: AAS56785.1. BK006936 Genomic DNA. Translation: DAA07099.1.
PIR	A28123.
RefSeq	NP_009532.1. NM_001178261.1.
3D structure databases
ProteinModelPortal	P13434.
SMR	P13434. Positions 41-126.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1362N.
IntAct	P13434. 4 interactions.
MINT	MINT-567883.
STRING	P13434.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBL021C; YBL021C; YBL021C.
GeneID	852260.
KEGG	sce:YBL021C.
NMPDR	fig|4932.3.peg.225.
Organism-specific databases
CYGD	YBL021c.
SGD	S000000117. HAP3.
Phylogenomic databases
eggNOG	fuNOG11491.
GeneTree	EFGT00050000002013.
HOGENOM	HBG748799.
OMA	NTNESEH.
OrthoDB	EOG4N33Z8.
Gene expression databases
ArrayExpress	P13434.
Genevestigator	P13434.
GermOnline	YBL021C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR003958. CBFA_NFYB_domain. IPR009072. Histone-fold. IPR003957. Transcrpt_fac_CBFA/NFYB_topo. IPR003956. Transcrpt_fac_NFYB/HAP3_CS. [Graphical view]
Gene3D	G3DSA:1.10.20.10. Histone-fold. 1 hit.
Pfam	PF00808. CBFD_NFYB_HMF. 1 hit. [Graphical view]
PRINTS	PR00615. CCAATSUBUNTA.
SUPFAM	SSF47113. Histone-fold. 1 hit.
PROSITE	PS00685. NFYB_HAP3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	970850.

Entry information

Entry name

HAP3_YEAST

Accession

Primary (citable) accession number: P13434
Secondary accession number(s): D6VPX9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 1, 1990
Last modified:	December 14, 2011
This is version 114 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program