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P13434 (HAP3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 132. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transcriptional activator HAP3
Alternative name(s):
UAS2 regulatory protein A
Gene names
Name:HAP3
Ordered Locus Names:YBL021C
ORF Names:YBL0441
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length144 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts a component of the CCAT-binding factor, which is a transcriptional activator and binds to the upstream activation site (UAS2) of the CYC1 gene and other genes involved in mitochondrial electron transport and activates their expression. Recognizes the sequence 5'-CCAAT-3'.

Subunit structure

Component of the CCAT-binding factor (CBF or HAP complex II), which consists of one copy each of HAP2, HAP3, HAP4 and HAP5. The assembly of the HAP2-HAP3-HAP5 heteromer (HAP complex I) occurs in a one-step pathway and its binding to DNA is a prerequisite for the association of HAP4. Ref.7

Subcellular location

Nucleus.

Miscellaneous

Present with 2510 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the NFYB/HAP3 subunit family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

HAP2P067744EBI-8157,EBI-8152
HAP5Q025165EBI-8157,EBI-8165

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 144144Transcriptional activator HAP3
PRO_0000204630

Regions

DNA binding42 – 487
Region69 – 8012Subunit association domain (SAD)

Sequences

Sequence LengthMass (Da)Tools
P13434 [UniParc].

Last modified January 1, 1990. Version 1.
Checksum: 7AB5027BAE420337

FASTA14416,154
        10         20         30         40         50         60 
MNTNESEHVS TSPEDTQENG GNASSSGSLQ QISTLREQDR WLPINNVARL MKNTLPPSAK 

        70         80         90        100        110        120 
VSKDAKECMQ ECVSELISFV TSEASDRCAA DKRKTINGED ILISLHALGF ENYAEVLKIY 

       130        140 
LAKYRQQQAL KNQLMYEQDD EEVP 

« Hide

References

« Hide 'large scale' references
[1]"The HAP3 regulatory locus of Saccharomyces cerevisiae encodes divergent overlapping transcripts."
Hahn S., Pinkham J., Wei R., Miller R., Guarente L.
Mol. Cell. Biol. 8:655-663(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC MYA-3516 / BWG1-7A.
[2]"PIM1 encodes a mitochondrial ATP-dependent protease that is required for mitochondrial function in the yeast Saccharomyces cerevisiae."
van Dyck L., Pearce D.A., Sherman F.
J. Biol. Chem. 269:238-242(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"Complete DNA sequence of yeast chromosome II."
Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. expand/collapse author list , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[6]"Mutations in yeast HAP2/HAP3 define a hybrid CCAAT box binding domain."
Xing Y., Fikes J.D., Guarente L.
EMBO J. 12:4647-4655(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS.
[7]"A multiprotein complex that interacts with RNA polymerase II elongator."
Li Y., Takagi Y., Jiang Y., Tokunaga M., Erdjument-Bromage H., Tempst P., Kornberg R.D.
J. Biol. Chem. 276:29628-29631(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE CCAT-BINDING FACTOR.
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]"Assembly of the Hap2p/Hap3p/Hap4p/Hap5p-DNA complex in Saccharomyces cerevisiae."
McNabb D.S., Pinto I.
Eukaryot. Cell 4:1829-1839(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: ASSEMBLY OF THE CCAT-BINDING FACTOR.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M20318 Genomic DNA. Translation: AAA53538.1.
X74544 Genomic DNA. Translation: CAA52633.1.
Z35782 Genomic DNA. Translation: CAA84840.1.
AY558459 Genomic DNA. Translation: AAS56785.1.
BK006936 Genomic DNA. Translation: DAA07099.1.
PIRA28123.
RefSeqNP_009532.1. NM_001178261.1.

3D structure databases

ProteinModelPortalP13434.
SMRP13434. Positions 36-126.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32677. 134 interactions.
DIPDIP-1362N.
IntActP13434. 2 interactions.
MINTMINT-567883.
STRING4932.YBL021C.

Proteomic databases

PaxDbP13434.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYBL021C; YBL021C; YBL021C.
GeneID852260.
KEGGsce:YBL021C.

Organism-specific databases

CYGDYBL021c.
SGDS000000117. HAP3.

Phylogenomic databases

eggNOGCOG2036.
GeneTreeENSGT00550000074689.
HOGENOMHOG000211832.
OMAYLAKQTM.
OrthoDBEOG7PGF3K.

Enzyme and pathway databases

BioCycYEAST:G3O-28924-MONOMER.

Gene expression databases

GenevestigatorP13434.

Family and domain databases

Gene3D1.10.20.10. 1 hit.
InterProIPR003958. CBFA_NFYB_domain.
IPR009072. Histone-fold.
IPR003957. Transcrpt_fac_NFYB/HAP3.
IPR003956. Transcrpt_fac_NFYB/HAP3_CS.
[Graphical view]
PANTHERPTHR11064:SF9. PTHR11064:SF9. 1 hit.
PfamPF00808. CBFD_NFYB_HMF. 1 hit.
[Graphical view]
PRINTSPR00615. CCAATSUBUNTA.
SUPFAMSSF47113. SSF47113. 1 hit.
PROSITEPS00685. NFYB_HAP3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio970850.
PROP13434.

Entry information

Entry nameHAP3_YEAST
AccessionPrimary (citable) accession number: P13434
Secondary accession number(s): D6VPX9
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: April 16, 2014
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families