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P13434

- HAP3_YEAST

UniProt

P13434 - HAP3_YEAST

Protein

Transcriptional activator HAP3

Gene

HAP3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 137 (01 Oct 2014)
      Sequence version 1 (01 Jan 1990)
      Previous versions | rss
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    Functioni

    Acts a component of the CCAT-binding factor, which is a transcriptional activator and binds to the upstream activation site (UAS2) of the CYC1 gene and other genes involved in mitochondrial electron transport and activates their expression. Recognizes the sequence 5'-CCAAT-3'.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi42 – 487

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. sequence-specific DNA binding Source: InterPro

    GO - Biological processi

    1. carbon catabolite activation of transcription from RNA polymerase II promoter Source: SGD
    2. regulation of cellular respiration Source: SGD
    3. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-28924-MONOMER.
    ReactomeiREACT_108597. ATF6-alpha activates chaperone genes.
    REACT_188982. Activation of gene expression by SREBF (SREBP).
    REACT_188984. PPARA activates gene expression.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcriptional activator HAP3
    Alternative name(s):
    UAS2 regulatory protein A
    Gene namesi
    Name:HAP3
    Ordered Locus Names:YBL021C
    ORF Names:YBL0441
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome II

    Organism-specific databases

    CYGDiYBL021c.
    SGDiS000000117. HAP3.

    Subcellular locationi

    GO - Cellular componenti

    1. CCAAT-binding factor complex Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 144144Transcriptional activator HAP3PRO_0000204630Add
    BLAST

    Proteomic databases

    MaxQBiP13434.
    PaxDbiP13434.

    Expressioni

    Gene expression databases

    GenevestigatoriP13434.

    Interactioni

    Subunit structurei

    Component of the CCAT-binding factor (CBF or HAP complex II), which consists of one copy each of HAP2, HAP3, HAP4 and HAP5. The assembly of the HAP2-HAP3-HAP5 heteromer (HAP complex I) occurs in a one-step pathway and its binding to DNA is a prerequisite for the association of HAP4.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    HAP2P067744EBI-8157,EBI-8152
    HAP5Q025165EBI-8157,EBI-8165

    Protein-protein interaction databases

    BioGridi32677. 135 interactions.
    DIPiDIP-1362N.
    IntActiP13434. 2 interactions.
    MINTiMINT-567883.
    STRINGi4932.YBL021C.

    Structurei

    3D structure databases

    ProteinModelPortaliP13434.
    SMRiP13434. Positions 36-126.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni69 – 8012Subunit association domain (SAD)Add
    BLAST

    Sequence similaritiesi

    Belongs to the NFYB/HAP3 subunit family.Curated

    Phylogenomic databases

    eggNOGiCOG2036.
    GeneTreeiENSGT00550000074689.
    HOGENOMiHOG000211832.
    OMAiERSANQN.
    OrthoDBiEOG7PGF3K.

    Family and domain databases

    Gene3Di1.10.20.10. 1 hit.
    InterProiIPR003958. CBFA_NFYB_domain.
    IPR009072. Histone-fold.
    IPR003956. Transcrpt_fac_NFYB/HAP3_CS.
    [Graphical view]
    PfamiPF00808. CBFD_NFYB_HMF. 1 hit.
    [Graphical view]
    SUPFAMiSSF47113. SSF47113. 1 hit.
    PROSITEiPS00685. NFYB_HAP3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P13434-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNTNESEHVS TSPEDTQENG GNASSSGSLQ QISTLREQDR WLPINNVARL    50
    MKNTLPPSAK VSKDAKECMQ ECVSELISFV TSEASDRCAA DKRKTINGED 100
    ILISLHALGF ENYAEVLKIY LAKYRQQQAL KNQLMYEQDD EEVP 144
    Length:144
    Mass (Da):16,154
    Last modified:January 1, 1990 - v1
    Checksum:i7AB5027BAE420337
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M20318 Genomic DNA. Translation: AAA53538.1.
    X74544 Genomic DNA. Translation: CAA52633.1.
    Z35782 Genomic DNA. Translation: CAA84840.1.
    AY558459 Genomic DNA. Translation: AAS56785.1.
    BK006936 Genomic DNA. Translation: DAA07099.1.
    PIRiA28123.
    RefSeqiNP_009532.1. NM_001178261.1.

    Genome annotation databases

    EnsemblFungiiYBL021C; YBL021C; YBL021C.
    GeneIDi852260.
    KEGGisce:YBL021C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M20318 Genomic DNA. Translation: AAA53538.1 .
    X74544 Genomic DNA. Translation: CAA52633.1 .
    Z35782 Genomic DNA. Translation: CAA84840.1 .
    AY558459 Genomic DNA. Translation: AAS56785.1 .
    BK006936 Genomic DNA. Translation: DAA07099.1 .
    PIRi A28123.
    RefSeqi NP_009532.1. NM_001178261.1.

    3D structure databases

    ProteinModelPortali P13434.
    SMRi P13434. Positions 36-126.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32677. 135 interactions.
    DIPi DIP-1362N.
    IntActi P13434. 2 interactions.
    MINTi MINT-567883.
    STRINGi 4932.YBL021C.

    Proteomic databases

    MaxQBi P13434.
    PaxDbi P13434.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YBL021C ; YBL021C ; YBL021C .
    GeneIDi 852260.
    KEGGi sce:YBL021C.

    Organism-specific databases

    CYGDi YBL021c.
    SGDi S000000117. HAP3.

    Phylogenomic databases

    eggNOGi COG2036.
    GeneTreei ENSGT00550000074689.
    HOGENOMi HOG000211832.
    OMAi ERSANQN.
    OrthoDBi EOG7PGF3K.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-28924-MONOMER.
    Reactomei REACT_108597. ATF6-alpha activates chaperone genes.
    REACT_188982. Activation of gene expression by SREBF (SREBP).
    REACT_188984. PPARA activates gene expression.

    Miscellaneous databases

    NextBioi 970850.
    PROi P13434.

    Gene expression databases

    Genevestigatori P13434.

    Family and domain databases

    Gene3Di 1.10.20.10. 1 hit.
    InterProi IPR003958. CBFA_NFYB_domain.
    IPR009072. Histone-fold.
    IPR003956. Transcrpt_fac_NFYB/HAP3_CS.
    [Graphical view ]
    Pfami PF00808. CBFD_NFYB_HMF. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47113. SSF47113. 1 hit.
    PROSITEi PS00685. NFYB_HAP3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The HAP3 regulatory locus of Saccharomyces cerevisiae encodes divergent overlapping transcripts."
      Hahn S., Pinkham J., Wei R., Miller R., Guarente L.
      Mol. Cell. Biol. 8:655-663(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC MYA-3516 / BWG1-7A.
    2. "PIM1 encodes a mitochondrial ATP-dependent protease that is required for mitochondrial function in the yeast Saccharomyces cerevisiae."
      van Dyck L., Pearce D.A., Sherman F.
      J. Biol. Chem. 269:238-242(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. "Complete DNA sequence of yeast chromosome II."
      Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
      , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
      EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    6. "Mutations in yeast HAP2/HAP3 define a hybrid CCAAT box binding domain."
      Xing Y., Fikes J.D., Guarente L.
      EMBO J. 12:4647-4655(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS.
    7. "A multiprotein complex that interacts with RNA polymerase II elongator."
      Li Y., Takagi Y., Jiang Y., Tokunaga M., Erdjument-Bromage H., Tempst P., Kornberg R.D.
      J. Biol. Chem. 276:29628-29631(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE CCAT-BINDING FACTOR.
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. "Assembly of the Hap2p/Hap3p/Hap4p/Hap5p-DNA complex in Saccharomyces cerevisiae."
      McNabb D.S., Pinto I.
      Eukaryot. Cell 4:1829-1839(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: ASSEMBLY OF THE CCAT-BINDING FACTOR.

    Entry informationi

    Entry nameiHAP3_YEAST
    AccessioniPrimary (citable) accession number: P13434
    Secondary accession number(s): D6VPX9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: January 1, 1990
    Last modified: October 1, 2014
    This is version 137 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 2510 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome II
      Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

    External Data

    Dasty 3