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Protein

Transcriptional activator HAP3

Gene

HAP3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts a component of the CCAT-binding factor, which is a transcriptional activator and binds to the upstream activation site (UAS2) of the CYC1 gene and other genes involved in mitochondrial electron transport and activates their expression. Recognizes the sequence 5'-CCAAT-3'.

Miscellaneous

Present with 2510 molecules/cell in log phase SD medium.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi42 – 487

GO - Molecular functioni

GO - Biological processi

  • carbon catabolite activation of transcription from RNA polymerase II promoter Source: SGD
  • regulation of cellular respiration Source: SGD
  • transcription by RNA polymerase II Source: GOC

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-28924-MONOMER
ReactomeiR-SCE-110314 Recognition of DNA damage by PCNA-containing replication complex
R-SCE-174430 Telomere C-strand synthesis initiation
R-SCE-5651801 PCNA-Dependent Long Patch Base Excision Repair
R-SCE-5656169 Termination of translesion DNA synthesis
R-SCE-5696400 Dual Incision in GG-NER
R-SCE-6782135 Dual incision in TC-NER
R-SCE-68952 DNA replication initiation
R-SCE-68962 Activation of the pre-replicative complex

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional activator HAP3
Alternative name(s):
UAS2 regulatory protein A
Gene namesi
Name:HAP3
Ordered Locus Names:YBL021C
ORF Names:YBL0441
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBL021C
SGDiS000000117 HAP3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002046301 – 144Transcriptional activator HAP3Add BLAST144

Proteomic databases

MaxQBiP13434
PaxDbiP13434
PRIDEiP13434

Interactioni

Subunit structurei

Component of the CCAT-binding factor (CBF or HAP complex II), which consists of one copy each of HAP2, HAP3, HAP4 and HAP5. The assembly of the HAP2-HAP3-HAP5 heteromer (HAP complex I) occurs in a one-step pathway and its binding to DNA is a prerequisite for the association of HAP4.1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi32677, 363 interactors
DIPiDIP-1362N
IntActiP13434, 19 interactors
MINTiP13434
STRINGi4932.YBL021C

Structurei

3D structure databases

ProteinModelPortaliP13434
SMRiP13434
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni69 – 80Subunit association domain (SAD)Add BLAST12

Sequence similaritiesi

Belongs to the NFYB/HAP3 subunit family.Curated

Phylogenomic databases

GeneTreeiENSGT00550000074689
HOGENOMiHOG000211832
InParanoidiP13434
OMAiRIMKASV
OrthoDBiEOG092C55XT

Family and domain databases

Gene3Di1.10.20.10, 1 hit
InterProiView protein in InterPro
IPR003958 CBFA_NFYB_domain
IPR009072 Histone-fold
IPR003956 Transcrpt_fac_NFYB/HAP3_CS
PfamiView protein in Pfam
PF00808 CBFD_NFYB_HMF, 1 hit
SUPFAMiSSF47113 SSF47113, 1 hit
PROSITEiView protein in PROSITE
PS00685 NFYB_HAP3, 1 hit

Sequencei

Sequence statusi: Complete.

P13434-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTNESEHVS TSPEDTQENG GNASSSGSLQ QISTLREQDR WLPINNVARL
60 70 80 90 100
MKNTLPPSAK VSKDAKECMQ ECVSELISFV TSEASDRCAA DKRKTINGED
110 120 130 140
ILISLHALGF ENYAEVLKIY LAKYRQQQAL KNQLMYEQDD EEVP
Length:144
Mass (Da):16,154
Last modified:January 1, 1990 - v1
Checksum:i7AB5027BAE420337
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20318 Genomic DNA Translation: AAA53538.1
X74544 Genomic DNA Translation: CAA52633.1
Z35782 Genomic DNA Translation: CAA84840.1
AY558459 Genomic DNA Translation: AAS56785.1
BK006936 Genomic DNA Translation: DAA07099.1
PIRiA28123
RefSeqiNP_009532.1, NM_001178261.1

Genome annotation databases

EnsemblFungiiYBL021C; YBL021C; YBL021C
GeneIDi852260
KEGGisce:YBL021C

Similar proteinsi

Entry informationi

Entry nameiHAP3_YEAST
AccessioniPrimary (citable) accession number: P13434
Secondary accession number(s): D6VPX9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: March 28, 2018
This is version 166 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health