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P13434

- HAP3_YEAST

UniProt

P13434 - HAP3_YEAST

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Protein

Transcriptional activator HAP3

Gene
HAP3, YBL021C, YBL0441
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Acts a component of the CCAT-binding factor, which is a transcriptional activator and binds to the upstream activation site (UAS2) of the CYC1 gene and other genes involved in mitochondrial electron transport and activates their expression. Recognizes the sequence 5'-CCAAT-3'.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi42 – 487

GO - Molecular functioni

  1. protein binding Source: IntAct
  2. sequence-specific DNA binding Source: InterPro

GO - Biological processi

  1. carbon catabolite activation of transcription from RNA polymerase II promoter Source: SGD
  2. regulation of cellular respiration Source: SGD
  3. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-28924-MONOMER.
ReactomeiREACT_108597. ATF6-alpha activates chaperone genes.
REACT_188982. Activation of gene expression by SREBF (SREBP).
REACT_188984. PPARA activates gene expression.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional activator HAP3
Alternative name(s):
UAS2 regulatory protein A
Gene namesi
Name:HAP3
Ordered Locus Names:YBL021C
ORF Names:YBL0441
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome II

Organism-specific databases

CYGDiYBL021c.
SGDiS000000117. HAP3.

Subcellular locationi

GO - Cellular componenti

  1. CCAAT-binding factor complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 144144Transcriptional activator HAP3PRO_0000204630Add
BLAST

Proteomic databases

MaxQBiP13434.
PaxDbiP13434.

Expressioni

Gene expression databases

GenevestigatoriP13434.

Interactioni

Subunit structurei

Component of the CCAT-binding factor (CBF or HAP complex II), which consists of one copy each of HAP2, HAP3, HAP4 and HAP5. The assembly of the HAP2-HAP3-HAP5 heteromer (HAP complex I) occurs in a one-step pathway and its binding to DNA is a prerequisite for the association of HAP4.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
HAP2P067744EBI-8157,EBI-8152
HAP5Q025165EBI-8157,EBI-8165

Protein-protein interaction databases

BioGridi32677. 135 interactions.
DIPiDIP-1362N.
IntActiP13434. 2 interactions.
MINTiMINT-567883.
STRINGi4932.YBL021C.

Structurei

3D structure databases

ProteinModelPortaliP13434.
SMRiP13434. Positions 36-126.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni69 – 8012Subunit association domain (SAD)Add
BLAST

Sequence similaritiesi

Belongs to the NFYB/HAP3 subunit family.

Phylogenomic databases

eggNOGiCOG2036.
GeneTreeiENSGT00550000074689.
HOGENOMiHOG000211832.
OMAiERSANQN.
OrthoDBiEOG7PGF3K.

Family and domain databases

Gene3Di1.10.20.10. 1 hit.
InterProiIPR003958. CBFA_NFYB_domain.
IPR009072. Histone-fold.
IPR003956. Transcrpt_fac_NFYB/HAP3_CS.
[Graphical view]
PfamiPF00808. CBFD_NFYB_HMF. 1 hit.
[Graphical view]
SUPFAMiSSF47113. SSF47113. 1 hit.
PROSITEiPS00685. NFYB_HAP3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P13434-1 [UniParc]FASTAAdd to Basket

« Hide

MNTNESEHVS TSPEDTQENG GNASSSGSLQ QISTLREQDR WLPINNVARL    50
MKNTLPPSAK VSKDAKECMQ ECVSELISFV TSEASDRCAA DKRKTINGED 100
ILISLHALGF ENYAEVLKIY LAKYRQQQAL KNQLMYEQDD EEVP 144
Length:144
Mass (Da):16,154
Last modified:January 1, 1990 - v1
Checksum:i7AB5027BAE420337
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M20318 Genomic DNA. Translation: AAA53538.1.
X74544 Genomic DNA. Translation: CAA52633.1.
Z35782 Genomic DNA. Translation: CAA84840.1.
AY558459 Genomic DNA. Translation: AAS56785.1.
BK006936 Genomic DNA. Translation: DAA07099.1.
PIRiA28123.
RefSeqiNP_009532.1. NM_001178261.1.

Genome annotation databases

EnsemblFungiiYBL021C; YBL021C; YBL021C.
GeneIDi852260.
KEGGisce:YBL021C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M20318 Genomic DNA. Translation: AAA53538.1 .
X74544 Genomic DNA. Translation: CAA52633.1 .
Z35782 Genomic DNA. Translation: CAA84840.1 .
AY558459 Genomic DNA. Translation: AAS56785.1 .
BK006936 Genomic DNA. Translation: DAA07099.1 .
PIRi A28123.
RefSeqi NP_009532.1. NM_001178261.1.

3D structure databases

ProteinModelPortali P13434.
SMRi P13434. Positions 36-126.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32677. 135 interactions.
DIPi DIP-1362N.
IntActi P13434. 2 interactions.
MINTi MINT-567883.
STRINGi 4932.YBL021C.

Proteomic databases

MaxQBi P13434.
PaxDbi P13434.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YBL021C ; YBL021C ; YBL021C .
GeneIDi 852260.
KEGGi sce:YBL021C.

Organism-specific databases

CYGDi YBL021c.
SGDi S000000117. HAP3.

Phylogenomic databases

eggNOGi COG2036.
GeneTreei ENSGT00550000074689.
HOGENOMi HOG000211832.
OMAi ERSANQN.
OrthoDBi EOG7PGF3K.

Enzyme and pathway databases

BioCyci YEAST:G3O-28924-MONOMER.
Reactomei REACT_108597. ATF6-alpha activates chaperone genes.
REACT_188982. Activation of gene expression by SREBF (SREBP).
REACT_188984. PPARA activates gene expression.

Miscellaneous databases

NextBioi 970850.
PROi P13434.

Gene expression databases

Genevestigatori P13434.

Family and domain databases

Gene3Di 1.10.20.10. 1 hit.
InterProi IPR003958. CBFA_NFYB_domain.
IPR009072. Histone-fold.
IPR003956. Transcrpt_fac_NFYB/HAP3_CS.
[Graphical view ]
Pfami PF00808. CBFD_NFYB_HMF. 1 hit.
[Graphical view ]
SUPFAMi SSF47113. SSF47113. 1 hit.
PROSITEi PS00685. NFYB_HAP3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The HAP3 regulatory locus of Saccharomyces cerevisiae encodes divergent overlapping transcripts."
    Hahn S., Pinkham J., Wei R., Miller R., Guarente L.
    Mol. Cell. Biol. 8:655-663(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC MYA-3516 / BWG1-7A.
  2. "PIM1 encodes a mitochondrial ATP-dependent protease that is required for mitochondrial function in the yeast Saccharomyces cerevisiae."
    van Dyck L., Pearce D.A., Sherman F.
    J. Biol. Chem. 269:238-242(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. "Mutations in yeast HAP2/HAP3 define a hybrid CCAAT box binding domain."
    Xing Y., Fikes J.D., Guarente L.
    EMBO J. 12:4647-4655(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS.
  7. "A multiprotein complex that interacts with RNA polymerase II elongator."
    Li Y., Takagi Y., Jiang Y., Tokunaga M., Erdjument-Bromage H., Tempst P., Kornberg R.D.
    J. Biol. Chem. 276:29628-29631(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE CCAT-BINDING FACTOR.
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "Assembly of the Hap2p/Hap3p/Hap4p/Hap5p-DNA complex in Saccharomyces cerevisiae."
    McNabb D.S., Pinto I.
    Eukaryot. Cell 4:1829-1839(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: ASSEMBLY OF THE CCAT-BINDING FACTOR.

Entry informationi

Entry nameiHAP3_YEAST
AccessioniPrimary (citable) accession number: P13434
Secondary accession number(s): D6VPX9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: September 3, 2014
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2510 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

External Data

Dasty 3