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Protein

DNA-directed RNA polymerase, mitochondrial

Gene

RPO41

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei945 – 9451By similarity
Active sitei1014 – 10141By similarity
Active sitei1189 – 11891By similarity

GO - Molecular functioni

  • DNA binding Source: InterPro
  • DNA-directed RNA polymerase activity Source: SGD
  • DNA primase activity Source: SGD

GO - Biological processi

  • DNA replication, synthesis of RNA primer Source: SGD
  • mitochondrial genome maintenance Source: SGD
  • transcription from mitochondrial promoter Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Transcription

Enzyme and pathway databases

BioCyciYEAST:G3O-30426-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase, mitochondrial (EC:2.7.7.6)
Gene namesi
Name:RPO41
Ordered Locus Names:YFL036W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VI

Organism-specific databases

EuPathDBiFungiDB:YFL036W.
SGDiS000001858. RPO41.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial DNA-directed RNA polymerase complex Source: SGD
  • mitochondrial matrix Source: SGD
  • mitochondrial nucleoid Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

DNA-directed RNA polymerase, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 1351DNA-directed RNA polymerase, mitochondrialPRO_0000031078
Transit peptidei1 – ?Mitochondrion

Proteomic databases

MaxQBiP13433.
PeptideAtlasiP13433.
PRIDEiP13433.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
itself1EBI-15960,EBI-15960

Protein-protein interaction databases

BioGridi31110. 120 interactions.
DIPiDIP-2431N.
IntActiP13433. 7 interactions.
MINTiMINT-500319.

Structurei

3D structure databases

ProteinModelPortaliP13433.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00390000008060.
HOGENOMiHOG000248537.
InParanoidiP13433.
KOiK10908.
OMAiHLIQDWL.
OrthoDBiEOG7PP5G0.

Family and domain databases

Gene3Di1.10.1320.10. 1 hit.
1.10.287.260. 1 hit.
InterProiIPR024075. DNA-dir_RNA_pol_helix_hairpin.
IPR002092. DNA-dir_Rpol_phage-type.
IPR029262. RPOL_N.
[Graphical view]
PANTHERiPTHR10102. PTHR10102. 3 hits.
PfamiPF00940. RNA_pol. 1 hit.
PF14700. RPOL_N. 1 hit.
[Graphical view]
SMARTiSM01311. RPOL_N. 1 hit.
[Graphical view]
PROSITEiPS00900. RNA_POL_PHAGE_1. 1 hit.
PS00489. RNA_POL_PHAGE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P13433-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRPAYKSLV KTSLLQRRLI SSKGSKLFKP SPDSTSTILI SEDPLVTGSS
60 70 80 90 100
PTSSTTSGII SSNDFPLFNK NRKDAKSSIS YQWKNPSELE FDPFNKSHAS
110 120 130 140 150
AVTSMTRTRD VMQLWSLLEA CLQSNLMKRA FSILESLYLV PEHKQRFIED
160 170 180 190 200
YNMYLNSFSK NDPNFPILKM NEKLTNDLET SFKDVNYNDK TLAIMIHHAL
210 220 230 240 250
NFHSTTSSML LKPIISAYLK MSVNGIREIF SCLDILTISD LNILMNDLKV
260 270 280 290 300
ITPSQLPNSV RPILESLTLS PTPVNNIENE EGLNKVEAEN DSKLHKASNA
310 320 330 340 350
SSDSIKKPSL DPLREVSFHG STEVLSKDAE KLIAVDTIGM RVIRHTLLGL
360 370 380 390 400
SLTPEQKEQI SKFKFDANDN VLKMKPTKND DNNNSINFFE IYNSLPTLEE
410 420 430 440 450
KKAFESALNI FNQDRQKVLE NRATEAARER WKHDFEEAKA RGDISIEKNL
460 470 480 490 500
NVKLWKWYNE MLPLVKEEIN HCRSLLSEKL SDKKGLNKVD TNRLGYGPYL
510 520 530 540 550
TLIDPGKMCV ITILELLKLN STGGVIEGMR TARAVISVGK AIEMEFRSEQ
560 570 580 590 600
VLKSESQAFR DVNKKSPEFK KLVQNAKSVF RSSQIEQSKI LWPQSIRARI
610 620 630 640 650
GSVLISMLIQ VAKVSVQGVD PVTKAKVHGE APAFAHGYQY HNGSKLGVLK
660 670 680 690 700
IHKTLIRQLN GERLIASVQP QLLPMLVEPK PWVNWRSGGY HYTQSTLLRT
710 720 730 740 750
KDSPEQVAYL KAASDNGDID RVYDGLNVLG RTPWTVNRKV FDVVSQVWNK
760 770 780 790 800
GEGFLDIPGA QDEMVLPPAP PKNSDPSILR AWKLQVKTIA NKFSSDRSNR
810 820 830 840 850
CDTNYKLEIA RAFLGEKLYF PHNLDFRGRA YPLSPHFNHL GNDMSRGLLI
860 870 880 890 900
FWHGKKLGPS GLKWLKIHLS NLFGFDKLPL KDRVAFTESH LQDIKDSAEN
910 920 930 940 950
PLTGDRWWTT ADKPWQALAT CFELNEVMKM DNPEEFISHQ PVHQDGTCNG
960 970 980 990 1000
LQHYAALGGD VEGATQVNLV PSDKPQDVYA HVARLVQKRL EIAAEKGDEN
1010 1020 1030 1040 1050
AKILKDKITR KVVKQTVMTN VYGVTYVGAT FQIAKQLSPI FDDRKESLDF
1060 1070 1080 1090 1100
SKYLTKHVFS AIRELFHSAH LIQDWLGESA KRISKSIRLD VDEKSFKNGN
1110 1120 1130 1140 1150
KPDFMSSVIW TTPLGLPIVQ PYREESKKQV ETNLQTVFIS DPFAVNPVNA
1160 1170 1180 1190 1200
RRQKAGLPPN FIHSLDASHM LLSAAECGKQ GLDFASVHDS YWTHASDIDT
1210 1220 1230 1240 1250
MNVVLREQFI KLHEVDLVLR LKEEFDQRYK NYVKIGKLKR STDLAQKIIR
1260 1270 1280 1290 1300
IRKDLSRKLG RSTTLADEIY FEKKRQELLN SPLIEDRNVG EKMVTTVSLF
1310 1320 1330 1340 1350
EDITDLDALE LENGGDENSG MSVLLPLRLP EIPPKGDFDV TVLRNSQYFF

S
Length:1,351
Mass (Da):153,047
Last modified:October 5, 2010 - v2
Checksum:i70A055CB71DB223E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti485 – 4851G → S in AAA35007 (PubMed:3308116).Curated
Sequence conflicti900 – 9012NP → KS in AAA35007 (PubMed:3308116).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17539 Genomic DNA. Translation: AAA35007.1.
D50617 Genomic DNA. Translation: BAA09203.1.
BK006940 Genomic DNA. Translation: DAA12404.1.
PIRiS56218.
RefSeqiNP_116617.1. NM_001179930.1.

Genome annotation databases

EnsemblFungiiBAA09203; BAA09203; BAA09203.
YFL036W; YFL036W; YFL036W.
GeneIDi850507.
KEGGisce:YFL036W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17539 Genomic DNA. Translation: AAA35007.1.
D50617 Genomic DNA. Translation: BAA09203.1.
BK006940 Genomic DNA. Translation: DAA12404.1.
PIRiS56218.
RefSeqiNP_116617.1. NM_001179930.1.

3D structure databases

ProteinModelPortaliP13433.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31110. 120 interactions.
DIPiDIP-2431N.
IntActiP13433. 7 interactions.
MINTiMINT-500319.

Proteomic databases

MaxQBiP13433.
PeptideAtlasiP13433.
PRIDEiP13433.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiBAA09203; BAA09203; BAA09203.
YFL036W; YFL036W; YFL036W.
GeneIDi850507.
KEGGisce:YFL036W.

Organism-specific databases

EuPathDBiFungiDB:YFL036W.
SGDiS000001858. RPO41.

Phylogenomic databases

GeneTreeiENSGT00390000008060.
HOGENOMiHOG000248537.
InParanoidiP13433.
KOiK10908.
OMAiHLIQDWL.
OrthoDBiEOG7PP5G0.

Enzyme and pathway databases

BioCyciYEAST:G3O-30426-MONOMER.

Miscellaneous databases

PROiP13433.

Family and domain databases

Gene3Di1.10.1320.10. 1 hit.
1.10.287.260. 1 hit.
InterProiIPR024075. DNA-dir_RNA_pol_helix_hairpin.
IPR002092. DNA-dir_Rpol_phage-type.
IPR029262. RPOL_N.
[Graphical view]
PANTHERiPTHR10102. PTHR10102. 3 hits.
PfamiPF00940. RNA_pol. 1 hit.
PF14700. RPOL_N. 1 hit.
[Graphical view]
SMARTiSM01311. RPOL_N. 1 hit.
[Graphical view]
PROSITEiPS00900. RNA_POL_PHAGE_1. 1 hit.
PS00489. RNA_POL_PHAGE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Yeast mitochondrial RNA polymerase is homologous to those encoded by bacteriophages T3 and T7."
    Masters B.S., Stohl L.L., Clayton D.A.
    Cell 51:89-99(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.

Entry informationi

Entry nameiRPOM_YEAST
AccessioniPrimary (citable) accession number: P13433
Secondary accession number(s): D6VTJ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: October 5, 2010
Last modified: June 8, 2016
This is version 142 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VI
    Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.