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Protein

Formate--tetrahydrofolate ligase

Gene

fhs

Organism
Clostridium acidurici (Gottschalkia acidurici)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate.UniRule annotation

Pathway: tetrahydrofolate interconversion

This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi65 – 728ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.3.4.3. 1454.
UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Formate--tetrahydrofolate ligaseUniRule annotation (EC:6.3.4.3UniRule annotation)
Alternative name(s):
Formyltetrahydrofolate synthetaseUniRule annotation
Short name:
FHSUniRule annotation
Short name:
FTHFSUniRule annotation
Gene namesi
Name:fhsUniRule annotation
OrganismiClostridium acidurici (Gottschalkia acidurici)
Taxonomic identifieri1556 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesGottschalkia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 556556Formate--tetrahydrofolate ligasePRO_0000199338Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP13419.
SMRiP13419. Positions 4-554.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the formate--tetrahydrofolate ligase family.UniRule annotation

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_01543. FTHFS.
InterProiIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P13419-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTDIQIAQE AQMKHIKDVA ELIDIHEDDL ELYGKYKAKV SLDVLDQLKD
60 70 80 90 100
KPDGKLVLVT AINPTPAGEG KTTTNIGLSM GLNKLGKKTS TALREPSLGP
110 120 130 140 150
SFGVKGGAAG GGYAQVVPMA DINLHFTGDF HAITSAHSLL AALVDNHLHH
160 170 180 190 200
GNALRIDTNR IVWKRVVDMN DRALRKIVVG LGGKAQGITR EDGFDITVAS
210 220 230 240 250
EIMAILCLAN DREDLKERLG NMVVAYNVDG DAVRAKDLEA QGALTLILKD
260 270 280 290 300
AINPNIVQTL ENTPAFIHGG PFANIAHGCN SVLATKLALK TGDYAVTEAG
310 320 330 340 350
FGADLGAEKF FDIKCRYAGL NPDVAVIVAT VRALKMHGGV AKEDLGTENL
360 370 380 390 400
DALAKGMTNL ERHIENVAKF GVPSVVAINA FPTDTEAEKQ LVFDKCKEMG
410 420 430 440 450
VDVAISDVFA KGGDGGVELA QKVIDVCENK KSDFKVLYDV EESIPEKITK
460 470 480 490 500
IAKEIYRADK VNFSKAAKKQ IAELEKLGLD KLPICMAKTQ YSFSDDPALL
510 520 530 540 550
GAPEGFELTI RDLELAAGAG FIVALTGDIM RMPGLPKVPA ANRMDVLPNG

EIIGLF
Length:556
Mass (Da):59,589
Last modified:January 1, 1990 - v1
Checksum:i72D9D485863F0334
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M21507 Genomic DNA. Translation: AAA53187.1.
PIRiA28185.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M21507 Genomic DNA. Translation: AAA53187.1.
PIRiA28185.

3D structure databases

ProteinModelPortaliP13419.
SMRiP13419. Positions 4-554.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00193.
BRENDAi6.3.4.3. 1454.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_01543. FTHFS.
InterProiIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Nucleotide sequence of the Clostridium acidiurici ('Clostridium acidi-urici') gene for 10-formyltetrahydrofolate synthetase shows extensive amino acid homology with the trifunctional enzyme C1-tetrahydrofolate synthase from Saccharomyces cerevisiae."
    Whitehead T.R., Rabinowitz J.C.
    J. Bacteriol. 170:3255-3261(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiFTHS_CLOAC
AccessioniPrimary (citable) accession number: P13419
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: April 1, 2015
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.