Reviewed, UniProtKB/Swiss-Prot P13419 (FTHS_CLOAC)

Names and origin

Protein names

Recommended name:
    Formate--tetrahydrofolate ligase
    EC=6.3.4.3
Alternative name(s):
    Formyltetrahydrofolate synthetase
      Short name=FHS
      Short name=FTHFS

Gene names

Name:

fhs

Organism

Clostridium acidi-urici

Taxonomic identifier

1556 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	556 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP MF_01543
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_01543
Sequence similarities	Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological process	folic acid and derivative biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP formate-tetrahydrofolate ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 556	556	Formate--tetrahydrofolate ligase HAMAP MF_01543		PRO_0000199338
Regions
	Nucleotide binding	65 – 72	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P13419-1 [UniParc]. Last modified January 1, 1990. Version 1. Checksum: 72D9D485863F0334	FASTA	556	59,589
	10 20 30 40 50 60 MKTDIQIAQE AQMKHIKDVA ELIDIHEDDL ELYGKYKAKV SLDVLDQLKD KPDGKLVLVT 70 80 90 100 110 120 AINPTPAGEG KTTTNIGLSM GLNKLGKKTS TALREPSLGP SFGVKGGAAG GGYAQVVPMA 130 140 150 160 170 180 DINLHFTGDF HAITSAHSLL AALVDNHLHH GNALRIDTNR IVWKRVVDMN DRALRKIVVG 190 200 210 220 230 240 LGGKAQGITR EDGFDITVAS EIMAILCLAN DREDLKERLG NMVVAYNVDG DAVRAKDLEA 250 260 270 280 290 300 QGALTLILKD AINPNIVQTL ENTPAFIHGG PFANIAHGCN SVLATKLALK TGDYAVTEAG 310 320 330 340 350 360 FGADLGAEKF FDIKCRYAGL NPDVAVIVAT VRALKMHGGV AKEDLGTENL DALAKGMTNL 370 380 390 400 410 420 ERHIENVAKF GVPSVVAINA FPTDTEAEKQ LVFDKCKEMG VDVAISDVFA KGGDGGVELA 430 440 450 460 470 480 QKVIDVCENK KSDFKVLYDV EESIPEKITK IAKEIYRADK VNFSKAAKKQ IAELEKLGLD 490 500 510 520 530 540 KLPICMAKTQ YSFSDDPALL GAPEGFELTI RDLELAAGAG FIVALTGDIM RMPGLPKVPA 550 ANRMDVLPNG EIIGLF

References

[1]

"Nucleotide sequence of the Clostridium acidiurici ('Clostridium acidi-urici') gene for 10-formyltetrahydrofolate synthetase shows extensive amino acid homology with the trifunctional enzyme C1-tetrahydrofolate synthase from Saccharomyces cerevisiae." Whitehead T.R., Rabinowitz J.C. J. Bacteriol. 170:3255-3261(1988) [PubMed: 2838464] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M21507 Genomic DNA. Translation: AAA53187.1.
PIR	A28185.
3D structure databases
SMR	P13419. Positions 4-554.
ModBase	Search...
Enzyme and pathway databases
BRENDA	6.3.4.3. 858.
Family and domain databases
HAMAP	MF_01543. FTHFS. [Tree]
InterPro	IPR000559. Formate_THF_ligase. IPR020628. Formate_THF_ligase_CS. [Graphical view]
Pfam	PF01268. FTHFS. 1 hit. [Graphical view]
PROSITE	PS00721. FTHFS_1. 1 hit. PS00722. FTHFS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FTHS_CLOAC

Accession

Primary (citable) accession number: P13419

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 1, 1990
Last modified:	January 19, 2010
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)