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P13418

- POLS_CRPVC

UniProt

P13418 - POLS_CRPVC

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Protein

Structural polyprotein

Gene
N/A
Organism
Cricket paralysis virus (isolate Teleogryllus commodus/Australia/CrPVVIC/1968) (CrPV)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Structural polyprotein: precursor of all the viral capsid proteins.
Capsid protein 1, together with capsid proteins 2 and 3, form an icosahedral capsid protecting the viral RNA genome. The icosahedral capsid has a pseudo-T=3 symmetry with a diameter of approximately 300 Angstroms, and is composed of 60 copies of each CP1, CP2, and CP3. CP1 is situated at the 12 fivefold axes, whereas CP2 and CP3 are located at the quasi-sixfold axes. All these proteins contain a beta-sheet structure called beta-barrel jelly roll.
Capsid protein 4 is a tstructural component of the icosahedral capsid protecting the genomic RNA. It may play an important role in capsid assembly.
Capsid protein 2, together with capsid proteins 1 and 3, form an icosahedral capsid protecting the viral RNA genome. The icosahedral capsid has a pseudo-T=3 symmetry with a diameter of approximately 300 Angstroms, and is composed of 60 copies of each CP1, CP2, and CP3. CP1 is situated at the 12 fivefold axes, whereas CP2 and CP3 are located at the quasi-sixfold axes. All these proteins contain a beta-sheet structure called beta-barrel jelly roll.
Capsid protein 3, together with capsid proteins 1 and 2, form an icosahedral capsid protecting the viral RNA genome. The icosahedral capsid has a pseudo-T=3 symmetry with a diameter of approximately 300 Angstroms, and is composed of 60 copies of each CP1, CP2, and CP3. CP1 is situated at the 12 fivefold axes, whereas CP2 and CP3 are located at the quasi-sixfold axes. All these proteins contain a beta-sheet structure called beta-barrel jelly roll.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei283 – 2842Cleavage
Sitei340 – 3412Cleavage
Sitei635 – 6362Cleavage

GO - Molecular functioni

  1. structural molecule activity Source: InterPro
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Structural polyprotein
Cleaved into the following 4 chains:
Alternative name(s):
CP1
Alternative name(s):
CP4
Alternative name(s):
CP2
Alternative name(s):
CP3
OrganismiCricket paralysis virus (isolate Teleogryllus commodus/Australia/CrPVVIC/1968) (CrPV)
Taxonomic identifieri928300 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stagePicornaviralesDicistroviridaeCripavirus
Virus hostiTeleogryllus oceanicus (black field cricket) [TaxID: 128161]
ProteomesiUP000008590: Genome

Subcellular locationi

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-KW
  2. viral capsid Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Host cytoplasm, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 895895Structural polyproteinPRO_0000123825Add
BLAST
Chaini1 – 283283Capsid protein 1PRO_0000398368Add
BLAST
Chaini284 – 34057Capsid protein 4PRO_0000398369Add
BLAST
Chaini341 – 635295Capsid protein 2PRO_0000398370Add
BLAST
Chaini636 – 895260Capsid protein 3PRO_0000398371Add
BLAST

Post-translational modificationi

Specific enzymatic cleavages in vivo yield mature proteins.

Structurei

Secondary structure

1
895
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi15 – 173
Beta strandi19 – 246
Beta strandi27 – 337
Beta strandi40 – 423
Helixi49 – 524
Turni53 – 564
Helixi64 – 674
Beta strandi72 – 809
Beta strandi88 – 947
Helixi96 – 994
Helixi102 – 1087
Beta strandi111 – 12515
Beta strandi131 – 14010
Helixi142 – 1443
Helixi146 – 1538
Helixi156 – 1605
Beta strandi162 – 1687
Turni169 – 1713
Beta strandi174 – 1796
Beta strandi184 – 1896
Turni190 – 1934
Beta strandi198 – 20912
Turni211 – 2133
Beta strandi217 – 23418
Helixi285 – 2939
Helixi305 – 3073
Beta strandi320 – 3234
Helixi331 – 3377
Beta strandi356 – 3594
Beta strandi363 – 3653
Turni384 – 3874
Helixi396 – 4005
Beta strandi404 – 4129
Beta strandi420 – 4256
Beta strandi431 – 4377
Beta strandi440 – 4445
Helixi446 – 4516
Beta strandi455 – 4595
Beta strandi461 – 4688
Beta strandi473 – 48311
Turni488 – 4903
Beta strandi491 – 4933
Helixi497 – 4993
Beta strandi500 – 50910
Beta strandi512 – 5176
Beta strandi522 – 5243
Helixi534 – 5363
Turni542 – 5454
Helixi546 – 5483
Beta strandi552 – 56413
Beta strandi570 – 58011
Beta strandi593 – 5975
Helixi603 – 61715
Beta strandi637 – 6393
Helixi645 – 6484
Turni649 – 6513
Helixi667 – 6726
Helixi680 – 6834
Beta strandi688 – 6969
Beta strandi702 – 7054
Beta strandi716 – 7183
Helixi725 – 7295
Helixi730 – 7323
Beta strandi733 – 7375
Beta strandi740 – 75112
Beta strandi756 – 7583
Beta strandi763 – 7686
Beta strandi770 – 7723
Helixi773 – 7786
Beta strandi785 – 7895
Beta strandi793 – 7953
Beta strandi801 – 8055
Helixi807 – 8104
Beta strandi811 – 8177
Beta strandi822 – 8243
Beta strandi831 – 8344
Helixi838 – 8425
Beta strandi850 – 8578
Turni861 – 8633
Beta strandi864 – 87411
Beta strandi879 – 8835
Beta strandi889 – 8913

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1B35X-ray2.40A636-895[»]
B8-243[»]
C341-622[»]
D284-340[»]
ProteinModelPortaliP13418.
SMRiP13418. Positions 8-243, 284-622, 636-895.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP13418.

Family & Domainsi

Sequence similaritiesi

Belongs to the picornaviruses polyprotein family.Curated

Family and domain databases

Gene3Di2.60.120.20. 3 hits.
InterProiIPR014872. Dicistrovirus_capsid-polyPr_C.
IPR001676. Picornavirus_capsid.
IPR029053. Viral_coat.
IPR024343. VP4_dicistrovir.
[Graphical view]
PfamiPF08762. CRPV_capsid. 1 hit.
PF11492. Dicistro_VP4. 1 hit.
PF00073. Rhv. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P13418-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
ATFQDKQENS HIENEDKRLM SEQKEIVHFV SEGITPSTTA LPDIVNLSTN
60 70 80 90 100
YLDMTTREDR IHSIKDFLSG PIIIATNLWS SSDPVEKQLY TANFPEVLIS
110 120 130 140 150
NAMYQDKLKG FVGLRATLVV KVQVNSQPFQ QGRLMLQYIP YAQYMPNRVT
160 170 180 190 200
LINETLQGRS GCPTTDLELS VGTEVEMRIP YVSPHLYYNL ITGQGSFGSI
210 220 230 240 250
YVVVYSQLHD QVSGTGSIEY TVWAHLEDVD VQYPTGANIF TGNSPNYLSI
260 270 280 290 300
AERIATGDFT ETEMRKLWIH KTYLKRPARI YAQAAKELKQ LETNNSPSTA
310 320 330 340 350
LGQISEGLTT LSHIPVLGNI FSTPAWISAK AADLAKLFGF SKPTVQGKIG
360 370 380 390 400
ECKLRGQGRM ANFDGMDMSH KMALSSTNEI ETKEGLAGTS LDEMDLSRVL
410 420 430 440 450
SIPNYWDRFT WKTSDVTNTV LWDNYVSPFK VKPYSATITD RFRCTHMGYV
460 470 480 490 500
ANAFTYWRGS IVYTFKFVKT QYHSGRLRIS FIPYYYNTTI STGTPDVSRT
510 520 530 540 550
QKIVVDLRTS TEVSFTVPYI ASRPWLYCIR PESSWLSKDN KDGALMYNCV
560 570 580 590 600
SGIVRVEVLN QLVAAQNVFS EIDVICEVSG GPDLEFAGPT CPSYVPYAGD
610 620 630 640 650
LTLADTRKIE AERTQEYSNN EDNRITTQCS RIVAQVMGED QQIPRNEAQH
660 670 680 690 700
GVHPISIDTH RISNNWSPQA MCIGEKIVSI RQLIKRFGIF GDANTLQADG
710 720 730 740 750
SSFVVAPFTV TSPTKTLTST RNYTQFDYYY YLYAFWRGSM RIKMVAETQD
760 770 780 790 800
GTGTPRKKTN FTWFVRMFNS LQDSFNSLIS TSSSAVTTTV LPSGTINMGP
810 820 830 840 850
STQVIDPTVE GLIEVEVPYY NISHITPAVT IDDGTPSMED YLKGHSPPCL
860 870 880 890
LTFSPRDSIS ATNHHITASF MRAPGDDFSF MYLLEVPPLV NVARA
Length:895
Mass (Da):100,298
Last modified:October 11, 2004 - v2
Checksum:i92DA9084DDA850EE
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti445 – 4473THM → HTH in AAA42889. 1 PublicationCurated
Sequence conflicti451 – 4511A → T in AAA42889. 1 PublicationCurated
Sequence conflicti556 – 5561V → T in AAA42889. 1 PublicationCurated
Sequence conflicti579 – 5791S → N in AAA42889. 1 PublicationCurated
Sequence conflicti594 – 5952YV → MY in AAA42889. 1 PublicationCurated
Sequence conflicti601 – 6011L → D in AAA42889. 1 PublicationCurated
Sequence conflicti641 – 6422QQ → HE in AAA42889. 1 PublicationCurated
Sequence conflicti648 – 6481A → G in AAA42889. 1 PublicationCurated
Sequence conflicti678 – 6836VSIRQL → IDSTT in AAA42889. 1 PublicationCurated
Sequence conflicti746 – 7461A → V in AAA42889. 1 PublicationCurated
Sequence conflicti854 – 8552SP → A in AAA42889. 1 PublicationCurated
Sequence conflicti861 – 89535ATNHH…NVARA → TTHTRSYACAW in AAA42889. 1 PublicationCuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF218039 Genomic RNA. Translation: AAF80999.1.
M21938 Genomic RNA. Translation: AAA42889.1.
PIRiS28374.
RefSeqiNP_647482.1. NC_003924.1.

Genome annotation databases

GeneIDi944542.

Cross-referencesi

Web resourcesi

Virus Particle ExploreR db

Icosahedral capsid structure

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF218039 Genomic RNA. Translation: AAF80999.1 .
M21938 Genomic RNA. Translation: AAA42889.1 .
PIRi S28374.
RefSeqi NP_647482.1. NC_003924.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1B35 X-ray 2.40 A 636-895 [» ]
B 8-243 [» ]
C 341-622 [» ]
D 284-340 [» ]
ProteinModelPortali P13418.
SMRi P13418. Positions 8-243, 284-622, 636-895.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 944542.

Miscellaneous databases

EvolutionaryTracei P13418.

Family and domain databases

Gene3Di 2.60.120.20. 3 hits.
InterProi IPR014872. Dicistrovirus_capsid-polyPr_C.
IPR001676. Picornavirus_capsid.
IPR029053. Viral_coat.
IPR024343. VP4_dicistrovir.
[Graphical view ]
Pfami PF08762. CRPV_capsid. 1 hit.
PF11492. Dicistro_VP4. 1 hit.
PF00073. Rhv. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Naturally occurring dicistronic cricket paralysis virus RNA is regulated by two internal ribosome entry sites."
    Wilson J.E., Powell M.J., Hoover S.E., Sarnow P.
    Mol. Cell. Biol. 20:4990-4999(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Cloning of the genome of cricket paralysis virus: sequence of the 3' end."
    King L.A., Pullin J.S.K., Stanway G., Almond J.W., Moore N.F.
    Virus Res. 6:331-344(1987)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 444-895.
  3. "Translation initiation by factor-independent binding of eukaryotic ribosomes to internal ribosomal entry sites."
    Pisarev A.V., Shirokikh N.E., Hellen C.U.
    C. R. Biol. 328:589-605(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NON-METHIONINE TRANSLATION INITIATION.
  4. "Translation initiation factors are not required for Dicistroviridae IRES function in vivo."
    Deniz N., Lenarcic E.M., Landry D.M., Thompson S.R.
    RNA 15:932-946(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NON-METHIONINE TRANSLATION INITIATION.
  5. "The crystal structure of cricket paralysis virus: the first view of a new virus family."
    Tate J., Liljas L., Scotti P., Christian P., Lin T., Johnson J.E.
    Nat. Struct. Biol. 6:765-774(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 8-895, POLYPROTEIN PROTEOLYTIC CLEAVAGES.

Entry informationi

Entry nameiPOLS_CRPVC
AccessioniPrimary (citable) accession number: P13418
Secondary accession number(s): Q9IJX3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: October 11, 2004
Last modified: October 29, 2014
This is version 76 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Caution

Translation initiates on an Ala codon through an unusual Internal Ribosome Entry Site (IRES).Curated

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3