Reviewed, UniProtKB/Swiss-Prot P13394 (SCRB_VIBAL)

Names and origin

Protein names

Recommended name:
    Sucrose-6-phosphate hydrolase
      Short name=Sucrase
    EC=3.2.1.26
Alternative name(s):
    Invertase

Gene names

Name:

scrB

Organism

Vibrio alginolyticus

Taxonomic identifier

663 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	484 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Enables the bacterium to metabolize sucrose as a sole carbon source.
Catalytic activity	Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides.
Pathway	Glycan biosynthesis; sucrose metabolism.
Subcellular location	Cytoplasm.
Induction	By sucrose.
Sequence similarities	Belongs to the glycosyl hydrolase 32 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Cellular component	Cytoplasm
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	beta-fructofuranosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 484	484	Sucrose-6-phosphate hydrolase		PRO_0000169878
Regions
	Region	48 – 51	4	Substrate binding By similarity
	Region	110 – 111	2	Substrate binding By similarity
	Region	168 – 169	2	Substrate binding By similarity
Sites
	Active site	51	1	By similarity
	Binding site	67	1	Substrate By similarity
	Binding site	223	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P13394-1 [UniParc]. Last modified January 1, 1990. Version 1. Checksum: A521D006C2118BF4	FASTA	484	55,657
	10 20 30 40 50 60 MSLNNRWTVE QRYRRLEQIP QCDIEEMTLS RQQDKGFPSF HIAPKFGLLN DPNGLCYFNG 70 80 90 100 110 120 EHHIFYQWTP VGPVHGMKYW YHLSTKDFIH FTDHGVGLHP DQDYDSHGVY SGGALVENNQ 130 140 150 160 170 180 VLLFFTGNKR DQNWNRIPTQ CFATMDSDGS IEKHGVVIEN EHYTEHFRDP KVWKKGDDYL 190 200 210 220 230 240 MVVGAQTKTE HGSMALYQSK DLKTWQHKGP IKTKFSDLGY MWECPDFFEI NGQSVMLFSP 250 260 270 280 290 300 QGVSSSNPYD FKNIYSVAYI VGDQLNLESM TLENHQDILQ PDYGFDFYAP QTYLDESGRR 310 320 330 340 350 360 ILIAWIGLPE IDTPSVTHQW AGMLSLPREL TLKDGFLVQT PLPELKSLRK EEVVFAQSHT 370 380 390 400 410 420 LESTSCLIQL DLVGDGFELE LSNLKGDNIV FSATEHEFML DRRYMSHLYA EEFGGIRKAP 430 440 450 460 470 480 RLDAKQTIDI YIDNSVIEIF INGGKHTMTS RFFIDDLNKV TLKGLEQARL FPLKGITGLF ESAK

References

[1]	"Nucleotide sequence and analysis of the Vibrio alginolyticus sucrase gene (scrB)." Scholle R.R. Gene 80:49-56(1989) [PubMed: 2551785] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	M26511 Genomic DNA. Translation: AAA27562.1.
PIR	JU0091.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH32. Glycoside Hydrolase Family 32.
Enzyme and pathway databases
BRENDA	3.2.1.26. 39551.
Family and domain databases
InterPro	IPR001362. Glyco_hydro_32. IPR018053. Glyco_hydro_32_AS. IPR013189. Glyco_hydro_32_C. IPR013148. Glyco_hydro_32_N. IPR006232. Suc6P_hydrolase. [Graphical view]
Pfam	PF08244. Glyco_hydro_32C. 1 hit. PF00251. Glyco_hydro_32N. 1 hit. [Graphical view]
SMART	SM00640. Glyco_32. 1 hit. [Graphical view]
TIGRFAMs	TIGR01322. scrB_fam. 1 hit.
PROSITE	PS00609. GLYCOSYL_HYDROL_F32. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SCRB_VIBAL

Accession

Primary (citable) accession number: P13394

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 1, 1990
Last modified:	June 16, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)