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Protein

TATA-box-binding protein

Gene

SPT15

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

General transcription factor that functions at the core of the DNA-binding general transcription factor complex TFIID. Binding of TFIID to a promoter (with or without TATA element) is the initial step in preinitiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription.3 Publications

GO - Molecular functioni

GO - Biological processi

  • RNA polymerase III transcriptional preinitiation complex assembly Source: SGD
  • RNA polymerase II transcriptional preinitiation complex assembly Source: SGD
  • transcription elongation from RNA polymerase III promoter Source: Reactome
  • transcription from RNA polymerase III promoter Source: Reactome
  • transcription of nuclear large rRNA transcript from RNA polymerase I promoter Source: SGD
  • transposon integration Source: SGD
Complete GO annotation...

Keywords - Biological processi

Transcription

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30309-MONOMER.
ReactomeiR-SCE-674695. RNA Polymerase II Pre-transcription Events.
R-SCE-73776. RNA Polymerase II Promoter Escape.
R-SCE-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-SCE-73780. RNA Polymerase III Chain Elongation.
R-SCE-749476. RNA Polymerase III Abortive And Retractive Initiation.
R-SCE-75953. RNA Polymerase II Transcription Initiation.
R-SCE-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.
R-SCE-76061. RNA Polymerase III Transcription Initiation From Type 1 Promoter.
R-SCE-76066. RNA Polymerase III Transcription Initiation From Type 2 Promoter.
R-SCE-76071. RNA Polymerase III Transcription Initiation From Type 3 Promoter.

Names & Taxonomyi

Protein namesi
Recommended name:
TATA-box-binding protein
Alternative name(s):
TATA sequence-binding protein
Short name:
TBP
TATA-binding factor
TATA-box factor
Transcription factor D
Transcription initiation factor TFIID TBP subunit
Gene namesi
Name:SPT15
Synonyms:BTF1, TBP1
Ordered Locus Names:YER148W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER148W.
SGDiS000000950. SPT15.

Subcellular locationi

GO - Cellular componenti

  • nucleoplasm Source: Reactome
  • nucleus Source: SGD
  • transcription factor TFIID complex Source: SGD
  • transcription factor TFIIIB complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001539902 – 240TATA-box-binding proteinAdd BLAST239

Proteomic databases

MaxQBiP13393.
PRIDEiP13393.

PTM databases

iPTMnetiP13393.

Interactioni

Subunit structurei

Binds DNA as monomer. The 1.2 MDa TFIID complex is composed of TATA binding protein (TBP) and the 14 TBP-associated factors. One copy of each TAF1, TAF2, TAF3, TAF7, TAF8, TAF11, TAF13, two copies of each TAF4, TAF5, TAF6, TAF9, TAF10, TAF12, and three copies of TAF14. Interacts with TFC8.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
BRF1P290562EBI-19129,EBI-19142
KAP114P530673EBI-19129,EBI-9174
MOT1P323339EBI-19129,EBI-11152
RRN11Q047122EBI-19129,EBI-27790
RVB1Q039403EBI-19129,EBI-30712
RVB2Q124643EBI-19129,EBI-31814
SPT8P389153EBI-19129,EBI-17964
TOA2P327742EBI-19129,EBI-19323

GO - Molecular functioni

  • RNA polymerase II activating transcription factor binding Source: SGD
  • RNA polymerase I transcription factor binding Source: SGD

Protein-protein interaction databases

BioGridi36897. 166 interactors.
DIPiDIP-44N.
IntActiP13393. 66 interactors.
MINTiMINT-408820.

Structurei

Secondary structure

1240
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi66 – 75Combined sources10
Helixi82 – 88Combined sources7
Beta strandi89 – 93Combined sources5
Turni96 – 98Combined sources3
Beta strandi100 – 106Combined sources7
Turni107 – 110Combined sources4
Beta strandi111 – 115Combined sources5
Beta strandi119 – 128Combined sources10
Helixi129 – 146Combined sources18
Beta strandi153 – 165Combined sources13
Helixi172 – 178Combined sources7
Turni179 – 182Combined sources4
Beta strandi183 – 186Combined sources4
Turni187 – 189Combined sources3
Beta strandi191 – 196Combined sources6
Beta strandi198 – 200Combined sources3
Beta strandi203 – 206Combined sources4
Beta strandi210 – 219Combined sources10
Helixi220 – 236Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NGMX-ray2.95A/E/I/M61-240[»]
1NH2X-ray1.90A61-240[»]
1RM1X-ray2.50A1-240[»]
1TBANMR-B61-240[»]
1TBPX-ray2.60A/B62-240[»]
1YTBX-ray1.80A/B61-240[»]
1YTFX-ray2.50A61-240[»]
4B0AX-ray1.97A61-240[»]
4V1Nelectron microscopy7.80O61-240[»]
4V1Oelectron microscopy9.70O61-240[»]
5FMFelectron microscopy6.00Q61-240[»]
5FYWelectron microscopy4.35O1-240[»]
5FZ5electron microscopy8.80O1-240[»]
5SVAelectron microscopy15.30j1-240[»]
ProteinModelPortaliP13393.
SMRiP13393.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP13393.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati67 – 1431Add BLAST77
Repeati157 – 2342Add BLAST78

Sequence similaritiesi

Belongs to the TBP family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00410000025389.
HOGENOMiHOG000105162.
InParanoidiP13393.
KOiK03120.
OMAiMEGIQTH.
OrthoDBiEOG092C4LP2.

Family and domain databases

CDDicd04516. TBP_eukaryotes. 1 hit.
Gene3Di3.30.310.10. 2 hits.
HAMAPiMF_00408. TATA_bind_prot_arch. 1 hit.
InterProiIPR012295. Beta2_adaptin/TBP_C_dom.
IPR000814. TBP.
IPR030491. TBP_CS.
IPR033710. TBP_eukaryotic.
[Graphical view]
PANTHERiPTHR10126. PTHR10126. 1 hit.
PfamiPF00352. TBP. 2 hits.
[Graphical view]
PRINTSiPR00686. TIFACTORIID.
PROSITEiPS00351. TFIID. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P13393-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADEERLKEF KEANKIVFDP NTRQVWENQN RDGTKPATTF QSEEDIKRAA
60 70 80 90 100
PESEKDTSAT SGIVPTLQNI VATVTLGCRL DLKTVALHAR NAEYNPKRFA
110 120 130 140 150
AVIMRIREPK TTALIFASGK MVVTGAKSED DSKLASRKYA RIIQKIGFAA
160 170 180 190 200
KFTDFKIQNI VGSCDVKFPI RLEGLAFSHG TFSSYEPELF PGLIYRMVKP
210 220 230 240
KIVLLIFVSG KIVLTGAKQR EEIYQAFEAI YPVLSEFRKM
Length:240
Mass (Da):27,003
Last modified:January 23, 2007 - v3
Checksum:i50365FFF1D3718CE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti50A → T in AAA35146 (PubMed:2682626).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M27135 Genomic DNA. Translation: AAA35147.1.
X16860 Genomic DNA. Translation: CAA34751.1.
M26403 Genomic DNA. Translation: AAA35146.1.
M29459 Genomic DNA. Translation: AAA34458.1.
U18917 Genomic DNA. Translation: AAB64675.1.
BK006939 Genomic DNA. Translation: DAA07809.1.
PIRiA30366.
RefSeqiNP_011075.3. NM_001179038.3.

Genome annotation databases

EnsemblFungiiYER148W; YER148W; YER148W.
GeneIDi856891.
KEGGisce:YER148W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M27135 Genomic DNA. Translation: AAA35147.1.
X16860 Genomic DNA. Translation: CAA34751.1.
M26403 Genomic DNA. Translation: AAA35146.1.
M29459 Genomic DNA. Translation: AAA34458.1.
U18917 Genomic DNA. Translation: AAB64675.1.
BK006939 Genomic DNA. Translation: DAA07809.1.
PIRiA30366.
RefSeqiNP_011075.3. NM_001179038.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NGMX-ray2.95A/E/I/M61-240[»]
1NH2X-ray1.90A61-240[»]
1RM1X-ray2.50A1-240[»]
1TBANMR-B61-240[»]
1TBPX-ray2.60A/B62-240[»]
1YTBX-ray1.80A/B61-240[»]
1YTFX-ray2.50A61-240[»]
4B0AX-ray1.97A61-240[»]
4V1Nelectron microscopy7.80O61-240[»]
4V1Oelectron microscopy9.70O61-240[»]
5FMFelectron microscopy6.00Q61-240[»]
5FYWelectron microscopy4.35O1-240[»]
5FZ5electron microscopy8.80O1-240[»]
5SVAelectron microscopy15.30j1-240[»]
ProteinModelPortaliP13393.
SMRiP13393.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36897. 166 interactors.
DIPiDIP-44N.
IntActiP13393. 66 interactors.
MINTiMINT-408820.

PTM databases

iPTMnetiP13393.

Proteomic databases

MaxQBiP13393.
PRIDEiP13393.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYER148W; YER148W; YER148W.
GeneIDi856891.
KEGGisce:YER148W.

Organism-specific databases

EuPathDBiFungiDB:YER148W.
SGDiS000000950. SPT15.

Phylogenomic databases

GeneTreeiENSGT00410000025389.
HOGENOMiHOG000105162.
InParanoidiP13393.
KOiK03120.
OMAiMEGIQTH.
OrthoDBiEOG092C4LP2.

Enzyme and pathway databases

BioCyciYEAST:G3O-30309-MONOMER.
ReactomeiR-SCE-674695. RNA Polymerase II Pre-transcription Events.
R-SCE-73776. RNA Polymerase II Promoter Escape.
R-SCE-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-SCE-73780. RNA Polymerase III Chain Elongation.
R-SCE-749476. RNA Polymerase III Abortive And Retractive Initiation.
R-SCE-75953. RNA Polymerase II Transcription Initiation.
R-SCE-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.
R-SCE-76061. RNA Polymerase III Transcription Initiation From Type 1 Promoter.
R-SCE-76066. RNA Polymerase III Transcription Initiation From Type 2 Promoter.
R-SCE-76071. RNA Polymerase III Transcription Initiation From Type 3 Promoter.

Miscellaneous databases

EvolutionaryTraceiP13393.
PROiP13393.

Family and domain databases

CDDicd04516. TBP_eukaryotes. 1 hit.
Gene3Di3.30.310.10. 2 hits.
HAMAPiMF_00408. TATA_bind_prot_arch. 1 hit.
InterProiIPR012295. Beta2_adaptin/TBP_C_dom.
IPR000814. TBP.
IPR030491. TBP_CS.
IPR033710. TBP_eukaryotic.
[Graphical view]
PANTHERiPTHR10126. PTHR10126. 1 hit.
PfamiPF00352. TBP. 2 hits.
[Graphical view]
PRINTSiPR00686. TIFACTORIID.
PROSITEiPS00351. TFIID. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTBP_YEAST
AccessioniPrimary (citable) accession number: P13393
Secondary accession number(s): D3DM55
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 181 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.