P13393 (TBP_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
TATA-box-binding protein

Alternative name(s):
TATA sequence-binding protein
Short name=TBP
TATA-binding factor
TATA-box factor
Transcription factor D
Transcription initiation factor TFIID TBP subunit

Gene names

Name:	SPT15
Synonyms:	BTF1, TBP1
Ordered Locus Names:	YER148W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	240 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	General transcription factor that functions at the core of the DNA-binding general transcription factor complex TFIID. Binding of TFIID to a promoter (with or without TATA element) is the initial step in preinitiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription. Ref.10 Ref.11 Ref.12
Subunit structure	Binds DNA as monomer. The 1.2 MDa TFIID complex is composed of TATA binding protein (TBP) and the 14 TBP-associated factors. One copy of each TAF1, TAF2, TAF3, TAF7, TAF8, TAF11, TAF13, two copies of each TAF4, TAF5, TAF6, TAF9, TAF10, TAF12, and three copies of TAF14. Interacts with TFC8. Ref.13
Subcellular location	Nucleus.
Sequence similarities	Belongs to the TBP family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	Nucleus
Domain	Repeat
Ligand	DNA-binding
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	RNA polymerase III transcriptional preinitiation complex assembly Inferred from direct assay PubMed 1480467. Source: SGD transcription of nuclear large rRNA transcript from RNA polymerase I promoter Inferred from mutant phenotype PubMed 1586947. Source: SGD transposon integration Inferred from direct assay PubMed 10882723. Source: SGD
Cellular_component	transcription factor TFIID complex Inferred from direct assay PubMed 10788514PubMed 15448131. Source: SGD transcription factor TFIIIB complex Inferred from direct assay PubMed 1423590PubMed 1458536. Source: SGD
Molecular_function	DNA binding, bending Inferred from direct assay PubMed 1736286. Source: SGD RNA polymerase I transcription factor binding Inferred from direct assay PubMed 8895657. Source: SGD RNA polymerase I transcription factor recruiting transcription factor activity Inferred from direct assay PubMed 8895657. Source: SGD RNA polymerase II activating transcription factor binding Inferred from direct assay PubMed 12501245. Source: SGD RNA polymerase II core promoter sequence-specific DNA binding Inferred from direct assay Ref.3. Source: SGD RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly Inferred by curator. Source: SGD RNA polymerase III regulatory region DNA binding Inferred from direct assay PubMed 8662956. Source: SGD TFIIIB-type transcription factor activity Inferred by curator. Source: SGD chromatin binding Inferred from direct assay PubMed 19098311. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BRF1	P29056	2	EBI-19129,EBI-19142
MOT1	P32333	9	EBI-19129,EBI-11152
RRN11	Q04712	2	EBI-19129,EBI-27790
RVB1	Q03940	3	EBI-19129,EBI-30712
RVB2	Q12464	3	EBI-19129,EBI-31814
TOA2	P32774	2	EBI-19129,EBI-19323

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed

Chain

2 – 240

239

TATA-box-binding protein

PRO_0000153990

Regions

Repeat

67 – 143

77

1

Repeat

157 – 234

78

2

Amino acid modifications

Modified residue

42

1

Phosphoserine Ref.14

Experimental info

Sequence conflict

50

1

A → T in AAA35146. Ref.4

Secondary structure

1

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240

Helix Strand Turn

Details...

Beta strand

66 – 75

10

Helix

82 – 88

7

Beta strand

89 – 93

5

Turn

96 – 98

3

Beta strand

100 – 106

7

Turn

107 – 110

4

Beta strand

111 – 115

5

Beta strand

119 – 128

10

Helix

129 – 146

18

Beta strand

153 – 165

13

Helix

172 – 178

7

Turn

179 – 182

4

Beta strand

183 – 186

4

Turn

187 – 189

3

Beta strand

191 – 196

6

Beta strand

198 – 200

3

Beta strand

203 – 206

4

Beta strand

210 – 219

10

Helix

220 – 236

17

Sequences

	Sequence		Length	Mass (Da)
	P13393 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 50365FFF1D3718CE	FASTA	240	27,003
	10 20 30 40 50 60 MADEERLKEF KEANKIVFDP NTRQVWENQN RDGTKPATTF QSEEDIKRAA PESEKDTSAT 70 80 90 100 110 120 SGIVPTLQNI VATVTLGCRL DLKTVALHAR NAEYNPKRFA AVIMRIREPK TTALIFASGK 130 140 150 160 170 180 MVVTGAKSED DSKLASRKYA RIIQKIGFAA KFTDFKIQNI VGSCDVKFPI RLEGLAFSHG 190 200 210 220 230 240 TFSSYEPELF PGLIYRMVKP KIVLLIFVSG KIVLTGAKQR EEIYQAFEAI YPVLSEFRKM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation of the gene encoding the yeast TATA binding protein TFIID: a gene identical to the SPT15 suppressor of Ty element insertions." Hahn S., Buratowski S., Sharp P.A., Guarente L. Cell 58:1173-1181(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"SPT15, the gene encoding the yeast TATA binding factor TFIID, is required for normal transcription initiation in vivo." Eisenmann D.M., Dollard C., Winston F. Cell 58:1183-1191(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Cloning and structure of a yeast gene encoding a general transcription initiation factor TFIID that binds to the TATA box." Horikoshi M., Wang C.K., Fujii H., Cromlish J.A., Weil P.A., Roeder R.G. Nature 341:299-303(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
[4]	"Yeast TATA-box transcription factor gene." Schmidt M.C., Kao C., Pei R., Berk A.J. Proc. Natl. Acad. Sci. U.S.A. 86:7785-7789(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]	"Cloning of the gene encoding the yeast protein BTF1Y, which can substitute for the human TATA box-binding factor." Cavallini B., Faus I., Matthes H., Chipoulet J.M., Winsor B., Egly J.-M., Chambon P. Proc. Natl. Acad. Sci. U.S.A. 86:9803-9807(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[6]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W. Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[7]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[8]	"Cryptic initiation sequence revealed." Hoeijmakers J.H.J. Nature 343:417-418(1990) [PubMed] [Europe PMC] [Abstract] Cited for: REPEAT IN SEQUENCE.
[9]	"Cryptic initiation sequence revealed." Nagai K. Nature 343:418-418(1990) Cited for: REPEAT IN SEQUENCE.
[10]	"Molecular genetics of the RNA polymerase II general transcriptional machinery." Hampsey M. Microbiol. Mol. Biol. Rev. 62:465-503(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[11]	"Molecular characterization of Saccharomyces cerevisiae TFIID." Sanders S.L., Garbett K.A., Weil P.A. Mol. Cell. Biol. 22:6000-6013(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TFIID STOICHIOMETRY.
[12]	"Multi-protein complexes in eukaryotic gene transcription." Martinez E. Plant Mol. Biol. 50:925-947(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[13]	"Structure of the tau60/Delta tau91 subcomplex of yeast transcription factor IIIC: insights into preinitiation complex assembly." Mylona A., Fernandez-Tornero C., Legrand P., Haupt M., Sentenac A., Acker J., Mueller C.W. Mol. Cell 24:221-232(2006) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH TFC8.
[14]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-42, MASS SPECTROMETRY.
[15]	"Crystal structure of a yeast TBP/TATA-box complex." Kim Y., Geiger J.H., Hahn S., Sigler P.B. Nature 365:512-520(1993) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 61-240.
[16]	"Crystal structure of yeast TATA-binding protein and model for interaction with DNA." Chasman D.I., Flaherty K.M., Sharp P.A., Kornberg R.D. Proc. Natl. Acad. Sci. U.S.A. 90:8174-8178(1993) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF 61-240.
[17]	"Crystal structure of a yeast TFIIA/TBP/DNA complex." Tan S., Hunziker Y., Sargent D.F., Richmond T.J. Nature 381:127-134(1996) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 61-240.
[18]	"Solution structure of a TBP-TAF(II)230 complex: protein mimicry of the minor groove surface of the TATA box unwound by TBP." Liu D., Ishima R., Tong K.I., Bagby S., Kokubo T., Muhandiram D.R., Kay L.E., Nakatani Y., Ikura M. Cell 94:573-583(1998) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR OF 61-240.
[19]	"Mapping histone fold TAFs within yeast TFIID." Leurent C., Sanders S.L., Ruhlmann C., Mallouh V., Weil P.A., Kirschner D.B., Tora L., Schultz P. EMBO J. 21:3424-3433(2002) [PubMed] [Europe PMC] [Abstract] Cited for: 3D-STRUCTURE, ELECTRON MICROSCOPY OF TFIID.
[20]	"Crystal structure of a transcription factor IIIB core interface ternary complex." Juo Z.S., Kassavetis G.A., Wang J., Geiduschek E.P., Sigler P.B. Nature 422:534-539(2003) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.95 ANGSTROMS) OF 61-240 IN COMPLEX WITH BRF1.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M27135 Genomic DNA. Translation: AAA35147.1.
X16860 Genomic DNA. Translation: CAA34751.1.
M26403 Genomic DNA. Translation: AAA35146.1.
M29459 Genomic DNA. Translation: AAA34458.1.
U18917 Genomic DNA. Translation: AAB64675.1.
BK006939 Genomic DNA. Translation: DAA07809.1.

PIR

A30366.

RefSeq

NP_011075.3. NM_001179038.3.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1NGM	X-ray	2.95	A/E/I/M	61-240	[»]
1NH2	X-ray	1.90	A	61-239	[»]
1RM1	X-ray	2.50	A	1-240	[»]
1TBA	NMR	-	B	61-240	[»]
1TBP	X-ray	2.60	A/B	62-240	[»]
1YTB	X-ray	1.80	A/B	61-240	[»]
1YTF	X-ray	2.50	A	61-240	[»]

ProteinModelPortal

P13393.

SMR

P13393. Positions 61-240.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-44N.

IntAct

P13393. 60 interactions.

MINT

MINT-408820.

STRING

4932.YER148W.

Proteomic databases

PaxDb

P13393.

PeptideAtlas

P13393.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YER148W; YER148W; YER148W.

GeneID

856891.

KEGG

sce:YER148W.
sce:YER152C.

Organism-specific databases

SGD

S000000950. SPT15.

Phylogenomic databases

eggNOG

COG2101.

GeneTree

ENSGT00410000025389.

HOGENOM

HOG000105162.

KO

K03120.

OMA

FEAIYPV.

OrthoDB

EOG4VT969.

Enzyme and pathway databases

Reactome

REACT_83470. Gene Expression.
REACT_87991. Transcription.

Gene expression databases

ArrayExpress

P13393.

Genevestigator

P13393.

GermOnline

YER148W. Saccharomyces cerevisiae.

Family and domain databases

Gene3D

3.30.310.10. 2 hits.

InterPro

IPR012295. Beta2_adaptin/TBP_C_dom.
IPR000814. TBP.
[Graphical view]

PANTHER

PTHR10126. PTHR10126. 1 hit.

Pfam

PF00352. TBP. 2 hits.
[Graphical view]

PRINTS

PR00686. TIFACTORIID.

PROSITE

PS00351. TFIID. 2 hits.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P13393.

NextBio

983296.

Entry information

Entry name

TBP_YEAST

Accession

Primary (citable) accession number: P13393
Secondary accession number(s): D3DM55

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 144 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program