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P13393 (TBP_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 156. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
TATA-box-binding protein
Alternative name(s):
TATA sequence-binding protein
Short name=TBP
TATA-binding factor
TATA-box factor
Transcription factor D
Transcription initiation factor TFIID TBP subunit
Gene names
Name:SPT15
Synonyms:BTF1, TBP1
Ordered Locus Names:YER148W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length240 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

General transcription factor that functions at the core of the DNA-binding general transcription factor complex TFIID. Binding of TFIID to a promoter (with or without TATA element) is the initial step in preinitiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription. Ref.10 Ref.11 Ref.12

Subunit structure

Binds DNA as monomer. The 1.2 MDa TFIID complex is composed of TATA binding protein (TBP) and the 14 TBP-associated factors. One copy of each TAF1, TAF2, TAF3, TAF7, TAF8, TAF11, TAF13, two copies of each TAF4, TAF5, TAF6, TAF9, TAF10, TAF12, and three copies of TAF14. Interacts with TFC8. Ref.13

Subcellular location

Nucleus HAMAP-Rule MF_00408.

Sequence similarities

Belongs to the TBP family.

Ontologies

Keywords
   Biological processTranscription
   Cellular componentNucleus
   DomainRepeat
   LigandDNA-binding
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processRNA polymerase II transcriptional preinitiation complex assembly

Inferred from mutant phenotype PubMed 12840001. Source: SGD

RNA polymerase III transcriptional preinitiation complex assembly

Inferred from direct assay PubMed 1480467. Source: SGD

regulation of transcription from RNA polymerase I promoter

Inferred from direct assay PubMed 8895657. Source: GOC

regulation of transcription from RNA polymerase III promoter

Inferred by curator. Source: GOC

transcription of nuclear large rRNA transcript from RNA polymerase I promoter

Inferred from mutant phenotype PubMed 1586947. Source: SGD

transposon integration

Inferred from direct assay PubMed 10882723. Source: SGD

   Cellular_componentnucleoplasm

Traceable author statement. Source: Reactome

nucleus

Inferred from direct assay PubMed 9818719. Source: SGD

transcription factor TFIID complex

Inferred from direct assay PubMed 10788514PubMed 15448131. Source: SGD

transcription factor TFIIIB complex

Inferred from direct assay PubMed 1423590PubMed 1458536. Source: SGD

   Molecular_functionDNA binding, bending

Inferred from direct assay PubMed 1736286. Source: SGD

RNA polymerase I transcription factor binding

Inferred from direct assay PubMed 8895657. Source: SGD

RNA polymerase I transcription factor recruiting transcription factor activity

Inferred from direct assay PubMed 8895657. Source: SGD

RNA polymerase II activating transcription factor binding

Inferred from direct assay PubMed 12501245. Source: SGD

RNA polymerase II core promoter sequence-specific DNA binding

Inferred from direct assay Ref.3. Source: SGD

RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly

Inferred by curator. Source: SGD

RNA polymerase III regulatory region DNA binding

Inferred from direct assay PubMed 8662956. Source: SGD

TFIIIB-type transcription factor activity

Inferred by curator. Source: SGD

chromatin binding

Inferred from direct assay PubMed 19098311. Source: SGD

protein binding

Inferred from physical interaction PubMed 10788514PubMed 11805826PubMed 12576485PubMed 12805227PubMed 16429126PubMed 16888622PubMed 22562154PubMed 23267104PubMed 8083216PubMed 8340360PubMed 8702872PubMed 8887672. Source: IntAct

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.3
Chain2 – 240239TATA-box-binding protein HAMAP-Rule MF_00408
PRO_0000153990

Regions

Repeat67 – 143771 HAMAP-Rule MF_00408
Repeat157 – 234782 HAMAP-Rule MF_00408

Experimental info

Sequence conflict501A → T in AAA35146. Ref.4

Secondary structure

............................... 240
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P13393 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 50365FFF1D3718CE

FASTA24027,003
        10         20         30         40         50         60 
MADEERLKEF KEANKIVFDP NTRQVWENQN RDGTKPATTF QSEEDIKRAA PESEKDTSAT 

        70         80         90        100        110        120 
SGIVPTLQNI VATVTLGCRL DLKTVALHAR NAEYNPKRFA AVIMRIREPK TTALIFASGK 

       130        140        150        160        170        180 
MVVTGAKSED DSKLASRKYA RIIQKIGFAA KFTDFKIQNI VGSCDVKFPI RLEGLAFSHG 

       190        200        210        220        230        240 
TFSSYEPELF PGLIYRMVKP KIVLLIFVSG KIVLTGAKQR EEIYQAFEAI YPVLSEFRKM 

« Hide

References

« Hide 'large scale' references
[1]"Isolation of the gene encoding the yeast TATA binding protein TFIID: a gene identical to the SPT15 suppressor of Ty element insertions."
Hahn S., Buratowski S., Sharp P.A., Guarente L.
Cell 58:1173-1181(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"SPT15, the gene encoding the yeast TATA binding factor TFIID, is required for normal transcription initiation in vivo."
Eisenmann D.M., Dollard C., Winston F.
Cell 58:1183-1191(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Cloning and structure of a yeast gene encoding a general transcription initiation factor TFIID that binds to the TATA box."
Horikoshi M., Wang C.K., Fujii H., Cromlish J.A., Weil P.A., Roeder R.G.
Nature 341:299-303(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE OF 2-34; 122-162 AND 198-233, CLEAVAGE OF INITIATOR METHIONINE.
[4]"Yeast TATA-box transcription factor gene."
Schmidt M.C., Kao C., Pei R., Berk A.J.
Proc. Natl. Acad. Sci. U.S.A. 86:7785-7789(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]"Cloning of the gene encoding the yeast protein BTF1Y, which can substitute for the human TATA box-binding factor."
Cavallini B., Faus I., Matthes H., Chipoulet J.M., Winsor B., Egly J.-M., Chambon P.
Proc. Natl. Acad. Sci. U.S.A. 86:9803-9807(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[6]"The nucleotide sequence of Saccharomyces cerevisiae chromosome V."
Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. expand/collapse author list , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W.
Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[7]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[8]"Cryptic initiation sequence revealed."
Hoeijmakers J.H.J.
Nature 343:417-418(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: REPEAT IN SEQUENCE.
[9]"Cryptic initiation sequence revealed."
Nagai K.
Nature 343:418-418(1990)
Cited for: REPEAT IN SEQUENCE.
[10]"Molecular genetics of the RNA polymerase II general transcriptional machinery."
Hampsey M.
Microbiol. Mol. Biol. Rev. 62:465-503(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[11]"Molecular characterization of Saccharomyces cerevisiae TFIID."
Sanders S.L., Garbett K.A., Weil P.A.
Mol. Cell. Biol. 22:6000-6013(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TFIID STOICHIOMETRY.
[12]"Multi-protein complexes in eukaryotic gene transcription."
Martinez E.
Plant Mol. Biol. 50:925-947(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[13]"Structure of the tau60/Delta tau91 subcomplex of yeast transcription factor IIIC: insights into preinitiation complex assembly."
Mylona A., Fernandez-Tornero C., Legrand P., Haupt M., Sentenac A., Acker J., Mueller C.W.
Mol. Cell 24:221-232(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH TFC8.
[14]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[15]"Crystal structure of a yeast TBP/TATA-box complex."
Kim Y., Geiger J.H., Hahn S., Sigler P.B.
Nature 365:512-520(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 61-240.
[16]"Crystal structure of yeast TATA-binding protein and model for interaction with DNA."
Chasman D.I., Flaherty K.M., Sharp P.A., Kornberg R.D.
Proc. Natl. Acad. Sci. U.S.A. 90:8174-8178(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF 61-240.
[17]"Crystal structure of a yeast TFIIA/TBP/DNA complex."
Tan S., Hunziker Y., Sargent D.F., Richmond T.J.
Nature 381:127-134(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 61-240.
[18]"Solution structure of a TBP-TAF(II)230 complex: protein mimicry of the minor groove surface of the TATA box unwound by TBP."
Liu D., Ishima R., Tong K.I., Bagby S., Kokubo T., Muhandiram D.R., Kay L.E., Nakatani Y., Ikura M.
Cell 94:573-583(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 61-240.
[19]"Mapping histone fold TAFs within yeast TFIID."
Leurent C., Sanders S.L., Ruhlmann C., Mallouh V., Weil P.A., Kirschner D.B., Tora L., Schultz P.
EMBO J. 21:3424-3433(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: 3D-STRUCTURE, ELECTRON MICROSCOPY OF TFIID.
[20]"Crystal structure of a transcription factor IIIB core interface ternary complex."
Juo Z.S., Kassavetis G.A., Wang J., Geiduschek E.P., Sigler P.B.
Nature 422:534-539(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.95 ANGSTROMS) OF 61-240 IN COMPLEX WITH BRF1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M27135 Genomic DNA. Translation: AAA35147.1.
X16860 Genomic DNA. Translation: CAA34751.1.
M26403 Genomic DNA. Translation: AAA35146.1.
M29459 Genomic DNA. Translation: AAA34458.1.
U18917 Genomic DNA. Translation: AAB64675.1.
BK006939 Genomic DNA. Translation: DAA07809.1.
PIRA30366.
RefSeqNP_011075.3. NM_001179038.3.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1NGMX-ray2.95A/E/I/M61-240[»]
1NH2X-ray1.90A61-240[»]
1RM1X-ray2.50A1-240[»]
1TBANMR-B61-240[»]
1TBPX-ray2.60A/B62-240[»]
1YTBX-ray1.80A/B61-240[»]
1YTFX-ray2.50A61-240[»]
4B0AX-ray1.97A61-240[»]
ProteinModelPortalP13393.
SMRP13393. Positions 61-240.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36897. 167 interactions.
DIPDIP-44N.
IntActP13393. 64 interactions.
MINTMINT-408820.
STRING4932.YER148W.

Proteomic databases

MaxQBP13393.
PaxDbP13393.
PeptideAtlasP13393.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYER148W; YER148W; YER148W.
GeneID856891.
KEGGsce:YER148W.

Organism-specific databases

SGDS000000950. SPT15.

Phylogenomic databases

eggNOGCOG2101.
GeneTreeENSGT00410000025389.
HOGENOMHOG000105162.
KOK03120.
OMAQAFEMIY.
OrthoDBEOG77T1GJ.

Enzyme and pathway databases

BioCycYEAST:G3O-30309-MONOMER.

Gene expression databases

GenevestigatorP13393.

Family and domain databases

Gene3D3.30.310.10. 2 hits.
HAMAPMF_00408. TATA_bind_prot_arch.
InterProIPR012295. Beta2_adaptin/TBP_C_dom.
IPR000814. TBP.
[Graphical view]
PANTHERPTHR10126. PTHR10126. 1 hit.
PfamPF00352. TBP. 2 hits.
[Graphical view]
PRINTSPR00686. TIFACTORIID.
PROSITEPS00351. TFIID. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP13393.
NextBio983296.

Entry information

Entry nameTBP_YEAST
AccessionPrimary (citable) accession number: P13393
Secondary accession number(s): D3DM55
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 156 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references