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Protein

TATA-box-binding protein

Gene

SPT15

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

General transcription factor that functions at the core of the DNA-binding general transcription factor complex TFIID. Binding of TFIID to a promoter (with or without TATA element) is the initial step in preinitiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription.3 Publications

GO - Molecular functioni

  • chromatin binding Source: SGD
  • disordered domain specific binding Source: CAFA
  • DNA binding, bending Source: SGD
  • RNA polymerase II activating transcription factor binding Source: SGD
  • RNA polymerase II core promoter sequence-specific DNA binding Source: SGD
  • RNA polymerase III regulatory region DNA binding Source: SGD
  • RNA polymerase I transcription factor binding Source: SGD
  • TFIIA-class transcription factor binding Source: CAFA
  • TFIIIB-type transcription factor activity Source: SGD
  • transcription factor activity, RNA polymerase II core promoter sequence-specific binding involved in preinitiation complex assembly Source: SGD
  • transcription factor activity, RNA polymerase I transcription factor recruiting Source: SGD
  • transcription factor binding Source: CAFA

GO - Biological processi

  • nucleolar large rRNA transcription by RNA polymerase I Source: SGD
  • RNA polymerase III transcriptional preinitiation complex assembly Source: SGD
  • RNA polymerase II transcriptional preinitiation complex assembly Source: SGD
  • transposon integration Source: SGD

Keywordsi

Molecular functionDNA-binding
Biological processTranscription

Enzyme and pathway databases

BioCyciYEAST:G3O-30309-MONOMER
ReactomeiR-SCE-674695 RNA Polymerase II Pre-transcription Events
R-SCE-73776 RNA Polymerase II Promoter Escape
R-SCE-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-SCE-75953 RNA Polymerase II Transcription Initiation
R-SCE-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance

Names & Taxonomyi

Protein namesi
Recommended name:
TATA-box-binding protein
Alternative name(s):
TATA sequence-binding protein
Short name:
TBP
TATA-binding factor
TATA-box factor
Transcription factor D
Transcription initiation factor TFIID TBP subunit
Gene namesi
Name:SPT15
Synonyms:BTF1, TBP1
Ordered Locus Names:YER148W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER148W
SGDiS000000950 SPT15

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001539902 – 240TATA-box-binding proteinAdd BLAST239

Proteomic databases

MaxQBiP13393
PaxDbiP13393
PRIDEiP13393

PTM databases

iPTMnetiP13393

Interactioni

Subunit structurei

Binds DNA as monomer. The 1.2 MDa TFIID complex is composed of TATA binding protein (TBP) and the 14 TBP-associated factors. One copy of each TAF1, TAF2, TAF3, TAF7, TAF8, TAF11, TAF13, two copies of each TAF4, TAF5, TAF6, TAF9, TAF10, TAF12, and three copies of TAF14. Interacts with TFC8.2 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • disordered domain specific binding Source: CAFA
  • RNA polymerase II activating transcription factor binding Source: SGD
  • RNA polymerase I transcription factor binding Source: SGD
  • TFIIA-class transcription factor binding Source: CAFA
  • transcription factor binding Source: CAFA

Protein-protein interaction databases

BioGridi36897, 309 interactors
DIPiDIP-44N
IntActiP13393, 84 interactors
MINTiP13393
STRINGi4932.YER148W

Structurei

Secondary structure

1240
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi66 – 75Combined sources10
Helixi82 – 88Combined sources7
Beta strandi89 – 93Combined sources5
Turni96 – 98Combined sources3
Beta strandi100 – 106Combined sources7
Turni107 – 110Combined sources4
Beta strandi111 – 115Combined sources5
Beta strandi119 – 128Combined sources10
Helixi129 – 146Combined sources18
Beta strandi153 – 165Combined sources13
Helixi172 – 178Combined sources7
Turni179 – 182Combined sources4
Beta strandi183 – 186Combined sources4
Turni187 – 189Combined sources3
Beta strandi191 – 196Combined sources6
Beta strandi198 – 200Combined sources3
Beta strandi203 – 206Combined sources4
Beta strandi210 – 219Combined sources10
Helixi220 – 236Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NGMX-ray2.95A/E/I/M61-240[»]
1NH2X-ray1.90A61-240[»]
1RM1X-ray2.50A1-240[»]
1TBANMR-B61-240[»]
1TBPX-ray2.60A/B62-240[»]
1YTBX-ray1.80A/B61-240[»]
1YTFX-ray2.50A61-240[»]
4B0AX-ray1.97A61-240[»]
4V1Nelectron microscopy7.80O61-240[»]
4V1Oelectron microscopy9.70O61-240[»]
5FMFelectron microscopy6.00Q61-240[»]
5FYWelectron microscopy4.35O1-240[»]
5FZ5electron microscopy8.80O1-240[»]
5SVAelectron microscopy15.30j1-240[»]
6EU0electron microscopy4.00Y1-240[»]
6F40electron microscopy3.70U1-240[»]
6F41electron microscopy4.30U1-240[»]
6F42electron microscopy5.50U1-240[»]
6F44electron microscopy4.20U1-240[»]
ProteinModelPortaliP13393
SMRiP13393
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP13393

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati67 – 1431Add BLAST77
Repeati157 – 2342Add BLAST78

Sequence similaritiesi

Belongs to the TBP family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00410000025389
HOGENOMiHOG000105162
InParanoidiP13393
KOiK03120
OMAiQAFEMIY
OrthoDBiEOG092C4LP2

Family and domain databases

CDDicd04516 TBP_eukaryotes, 1 hit
Gene3Di3.30.310.10, 2 hits
HAMAPiMF_00408 TATA_bind_prot_arch, 1 hit
InterProiView protein in InterPro
IPR000814 TBP
IPR030491 TBP_CS
IPR012295 TBP_dom_sf
IPR033710 TBP_eukaryotic
PANTHERiPTHR10126 PTHR10126, 1 hit
PfamiView protein in Pfam
PF00352 TBP, 2 hits
PRINTSiPR00686 TIFACTORIID
PROSITEiView protein in PROSITE
PS00351 TFIID, 2 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P13393-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADEERLKEF KEANKIVFDP NTRQVWENQN RDGTKPATTF QSEEDIKRAA
60 70 80 90 100
PESEKDTSAT SGIVPTLQNI VATVTLGCRL DLKTVALHAR NAEYNPKRFA
110 120 130 140 150
AVIMRIREPK TTALIFASGK MVVTGAKSED DSKLASRKYA RIIQKIGFAA
160 170 180 190 200
KFTDFKIQNI VGSCDVKFPI RLEGLAFSHG TFSSYEPELF PGLIYRMVKP
210 220 230 240
KIVLLIFVSG KIVLTGAKQR EEIYQAFEAI YPVLSEFRKM
Length:240
Mass (Da):27,003
Last modified:January 23, 2007 - v3
Checksum:i50365FFF1D3718CE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti50A → T in AAA35146 (PubMed:2682626).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M27135 Genomic DNA Translation: AAA35147.1
X16860 Genomic DNA Translation: CAA34751.1
M26403 Genomic DNA Translation: AAA35146.1
M29459 Genomic DNA Translation: AAA34458.1
U18917 Genomic DNA Translation: AAB64675.1
BK006939 Genomic DNA Translation: DAA07809.1
PIRiA30366
RefSeqiNP_011075.3, NM_001179038.3

Genome annotation databases

EnsemblFungiiYER148W; YER148W; YER148W
GeneIDi856891
KEGGisce:YER148W

Similar proteinsi

Entry informationi

Entry nameiTBP_YEAST
AccessioniPrimary (citable) accession number: P13393
Secondary accession number(s): D3DM55
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 23, 2007
Last modified: April 25, 2018
This is version 195 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health