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P13393

- TBP_YEAST

UniProt

P13393 - TBP_YEAST

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Protein

TATA-box-binding protein

Gene

SPT15

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

General transcription factor that functions at the core of the DNA-binding general transcription factor complex TFIID. Binding of TFIID to a promoter (with or without TATA element) is the initial step in preinitiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription.3 Publications

GO - Molecular functioni

  1. chromatin binding Source: SGD
  2. DNA binding, bending Source: SGD
  3. RNA polymerase II activating transcription factor binding Source: SGD
  4. RNA polymerase II core promoter sequence-specific DNA binding Source: SGD
  5. RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly Source: SGD
  6. RNA polymerase III regulatory region DNA binding Source: SGD
  7. RNA polymerase I transcription factor binding Source: SGD
  8. RNA polymerase I transcription factor recruiting transcription factor activity Source: SGD
  9. TFIIIB-type transcription factor activity Source: SGD

GO - Biological processi

  1. regulation of transcription from RNA polymerase III promoter Source: GOC
  2. regulation of transcription from RNA polymerase I promoter Source: GOC
  3. RNA polymerase III transcriptional preinitiation complex assembly Source: SGD
  4. RNA polymerase II transcriptional preinitiation complex assembly Source: SGD
  5. transcription of nuclear large rRNA transcript from RNA polymerase I promoter Source: SGD
  6. transposon integration Source: SGD
Complete GO annotation...

Keywords - Biological processi

Transcription

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30309-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
TATA-box-binding protein
Alternative name(s):
TATA sequence-binding protein
Short name:
TBP
TATA-binding factor
TATA-box factor
Transcription factor D
Transcription initiation factor TFIID TBP subunit
Gene namesi
Name:SPT15
Synonyms:BTF1, TBP1
Ordered Locus Names:YER148W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome V

Organism-specific databases

SGDiS000000950. SPT15.

Subcellular locationi

GO - Cellular componenti

  1. nucleoplasm Source: Reactome
  2. nucleus Source: SGD
  3. transcription factor TFIID complex Source: SGD
  4. transcription factor TFIIIB complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 240239TATA-box-binding proteinPRO_0000153990Add
BLAST

Proteomic databases

MaxQBiP13393.
PaxDbiP13393.
PeptideAtlasiP13393.

Expressioni

Gene expression databases

GenevestigatoriP13393.

Interactioni

Subunit structurei

Binds DNA as monomer. The 1.2 MDa TFIID complex is composed of TATA binding protein (TBP) and the 14 TBP-associated factors. One copy of each TAF1, TAF2, TAF3, TAF7, TAF8, TAF11, TAF13, two copies of each TAF4, TAF5, TAF6, TAF9, TAF10, TAF12, and three copies of TAF14. Interacts with TFC8.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
BRF1P290562EBI-19129,EBI-19142
KAP114P530673EBI-19129,EBI-9174
MOT1P323339EBI-19129,EBI-11152
RRN11Q047122EBI-19129,EBI-27790
RVB1Q039403EBI-19129,EBI-30712
RVB2Q124643EBI-19129,EBI-31814
SPT8P389153EBI-19129,EBI-17964
TOA2P327742EBI-19129,EBI-19323

Protein-protein interaction databases

BioGridi36897. 168 interactions.
DIPiDIP-44N.
IntActiP13393. 64 interactions.
MINTiMINT-408820.
STRINGi4932.YER148W.

Structurei

Secondary structure

1
240
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi66 – 7510
Helixi82 – 887
Beta strandi89 – 935
Turni96 – 983
Beta strandi100 – 1067
Turni107 – 1104
Beta strandi111 – 1155
Beta strandi119 – 12810
Helixi129 – 14618
Beta strandi153 – 16513
Helixi172 – 1787
Turni179 – 1824
Beta strandi183 – 1864
Turni187 – 1893
Beta strandi191 – 1966
Beta strandi198 – 2003
Beta strandi203 – 2064
Beta strandi210 – 21910
Helixi220 – 23617

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1NGMX-ray2.95A/E/I/M61-240[»]
1NH2X-ray1.90A61-240[»]
1RM1X-ray2.50A1-240[»]
1TBANMR-B61-240[»]
1TBPX-ray2.60A/B62-240[»]
1YTBX-ray1.80A/B61-240[»]
1YTFX-ray2.50A61-240[»]
4B0AX-ray1.97A61-240[»]
ProteinModelPortaliP13393.
SMRiP13393. Positions 61-240.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP13393.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati67 – 143771Add
BLAST
Repeati157 – 234782Add
BLAST

Sequence similaritiesi

Belongs to the TBP family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2101.
GeneTreeiENSGT00410000025389.
HOGENOMiHOG000105162.
InParanoidiP13393.
KOiK03120.
OMAiQAFEMIY.
OrthoDBiEOG77T1GJ.

Family and domain databases

Gene3Di3.30.310.10. 2 hits.
HAMAPiMF_00408. TATA_bind_prot_arch.
InterProiIPR012295. Beta2_adaptin/TBP_C_dom.
IPR000814. TBP.
[Graphical view]
PANTHERiPTHR10126. PTHR10126. 1 hit.
PfamiPF00352. TBP. 2 hits.
[Graphical view]
PRINTSiPR00686. TIFACTORIID.
PROSITEiPS00351. TFIID. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P13393-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MADEERLKEF KEANKIVFDP NTRQVWENQN RDGTKPATTF QSEEDIKRAA
60 70 80 90 100
PESEKDTSAT SGIVPTLQNI VATVTLGCRL DLKTVALHAR NAEYNPKRFA
110 120 130 140 150
AVIMRIREPK TTALIFASGK MVVTGAKSED DSKLASRKYA RIIQKIGFAA
160 170 180 190 200
KFTDFKIQNI VGSCDVKFPI RLEGLAFSHG TFSSYEPELF PGLIYRMVKP
210 220 230 240
KIVLLIFVSG KIVLTGAKQR EEIYQAFEAI YPVLSEFRKM
Length:240
Mass (Da):27,003
Last modified:January 23, 2007 - v3
Checksum:i50365FFF1D3718CE
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti50 – 501A → T in AAA35146. (PubMed:2682626)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M27135 Genomic DNA. Translation: AAA35147.1.
X16860 Genomic DNA. Translation: CAA34751.1.
M26403 Genomic DNA. Translation: AAA35146.1.
M29459 Genomic DNA. Translation: AAA34458.1.
U18917 Genomic DNA. Translation: AAB64675.1.
BK006939 Genomic DNA. Translation: DAA07809.1.
PIRiA30366.
RefSeqiNP_011075.3. NM_001179038.3.

Genome annotation databases

EnsemblFungiiYER148W; YER148W; YER148W.
GeneIDi856891.
KEGGisce:YER148W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M27135 Genomic DNA. Translation: AAA35147.1 .
X16860 Genomic DNA. Translation: CAA34751.1 .
M26403 Genomic DNA. Translation: AAA35146.1 .
M29459 Genomic DNA. Translation: AAA34458.1 .
U18917 Genomic DNA. Translation: AAB64675.1 .
BK006939 Genomic DNA. Translation: DAA07809.1 .
PIRi A30366.
RefSeqi NP_011075.3. NM_001179038.3.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1NGM X-ray 2.95 A/E/I/M 61-240 [» ]
1NH2 X-ray 1.90 A 61-240 [» ]
1RM1 X-ray 2.50 A 1-240 [» ]
1TBA NMR - B 61-240 [» ]
1TBP X-ray 2.60 A/B 62-240 [» ]
1YTB X-ray 1.80 A/B 61-240 [» ]
1YTF X-ray 2.50 A 61-240 [» ]
4B0A X-ray 1.97 A 61-240 [» ]
ProteinModelPortali P13393.
SMRi P13393. Positions 61-240.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36897. 168 interactions.
DIPi DIP-44N.
IntActi P13393. 64 interactions.
MINTi MINT-408820.
STRINGi 4932.YER148W.

Proteomic databases

MaxQBi P13393.
PaxDbi P13393.
PeptideAtlasi P13393.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YER148W ; YER148W ; YER148W .
GeneIDi 856891.
KEGGi sce:YER148W.

Organism-specific databases

SGDi S000000950. SPT15.

Phylogenomic databases

eggNOGi COG2101.
GeneTreei ENSGT00410000025389.
HOGENOMi HOG000105162.
InParanoidi P13393.
KOi K03120.
OMAi QAFEMIY.
OrthoDBi EOG77T1GJ.

Enzyme and pathway databases

BioCyci YEAST:G3O-30309-MONOMER.

Miscellaneous databases

EvolutionaryTracei P13393.
NextBioi 983296.

Gene expression databases

Genevestigatori P13393.

Family and domain databases

Gene3Di 3.30.310.10. 2 hits.
HAMAPi MF_00408. TATA_bind_prot_arch.
InterProi IPR012295. Beta2_adaptin/TBP_C_dom.
IPR000814. TBP.
[Graphical view ]
PANTHERi PTHR10126. PTHR10126. 1 hit.
Pfami PF00352. TBP. 2 hits.
[Graphical view ]
PRINTSi PR00686. TIFACTORIID.
PROSITEi PS00351. TFIID. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation of the gene encoding the yeast TATA binding protein TFIID: a gene identical to the SPT15 suppressor of Ty element insertions."
    Hahn S., Buratowski S., Sharp P.A., Guarente L.
    Cell 58:1173-1181(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "SPT15, the gene encoding the yeast TATA binding factor TFIID, is required for normal transcription initiation in vivo."
    Eisenmann D.M., Dollard C., Winston F.
    Cell 58:1183-1191(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Cloning and structure of a yeast gene encoding a general transcription initiation factor TFIID that binds to the TATA box."
    Horikoshi M., Wang C.K., Fujii H., Cromlish J.A., Weil P.A., Roeder R.G.
    Nature 341:299-303(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE OF 2-34; 122-162 AND 198-233, CLEAVAGE OF INITIATOR METHIONINE.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  5. "Cloning of the gene encoding the yeast protein BTF1Y, which can substitute for the human TATA box-binding factor."
    Cavallini B., Faus I., Matthes H., Chipoulet J.M., Winsor B., Egly J.-M., Chambon P.
    Proc. Natl. Acad. Sci. U.S.A. 86:9803-9807(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  7. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  8. "Cryptic initiation sequence revealed."
    Hoeijmakers J.H.J.
    Nature 343:417-418(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: REPEAT IN SEQUENCE.
  9. "Cryptic initiation sequence revealed."
    Nagai K.
    Nature 343:418-418(1990)
    Cited for: REPEAT IN SEQUENCE.
  10. "Molecular genetics of the RNA polymerase II general transcriptional machinery."
    Hampsey M.
    Microbiol. Mol. Biol. Rev. 62:465-503(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. "Molecular characterization of Saccharomyces cerevisiae TFIID."
    Sanders S.L., Garbett K.A., Weil P.A.
    Mol. Cell. Biol. 22:6000-6013(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TFIID STOICHIOMETRY.
  12. "Multi-protein complexes in eukaryotic gene transcription."
    Martinez E.
    Plant Mol. Biol. 50:925-947(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  13. "Structure of the tau60/Delta tau91 subcomplex of yeast transcription factor IIIC: insights into preinitiation complex assembly."
    Mylona A., Fernandez-Tornero C., Legrand P., Haupt M., Sentenac A., Acker J., Mueller C.W.
    Mol. Cell 24:221-232(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TFC8.
  14. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  15. "Crystal structure of a yeast TBP/TATA-box complex."
    Kim Y., Geiger J.H., Hahn S., Sigler P.B.
    Nature 365:512-520(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 61-240.
  16. "Crystal structure of yeast TATA-binding protein and model for interaction with DNA."
    Chasman D.I., Flaherty K.M., Sharp P.A., Kornberg R.D.
    Proc. Natl. Acad. Sci. U.S.A. 90:8174-8178(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF 61-240.
  17. "Crystal structure of a yeast TFIIA/TBP/DNA complex."
    Tan S., Hunziker Y., Sargent D.F., Richmond T.J.
    Nature 381:127-134(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 61-240.
  18. "Solution structure of a TBP-TAF(II)230 complex: protein mimicry of the minor groove surface of the TATA box unwound by TBP."
    Liu D., Ishima R., Tong K.I., Bagby S., Kokubo T., Muhandiram D.R., Kay L.E., Nakatani Y., Ikura M.
    Cell 94:573-583(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 61-240.
  19. Cited for: 3D-STRUCTURE, ELECTRON MICROSCOPY OF TFIID.
  20. "Crystal structure of a transcription factor IIIB core interface ternary complex."
    Juo Z.S., Kassavetis G.A., Wang J., Geiduschek E.P., Sigler P.B.
    Nature 422:534-539(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.95 ANGSTROMS) OF 61-240 IN COMPLEX WITH BRF1.

Entry informationi

Entry nameiTBP_YEAST
AccessioniPrimary (citable) accession number: P13393
Secondary accession number(s): D3DM55
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 158 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

External Data

Dasty 3