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P13393

- TBP_YEAST

UniProt

P13393 - TBP_YEAST

Protein

TATA-box-binding protein

Gene

SPT15

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 157 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    General transcription factor that functions at the core of the DNA-binding general transcription factor complex TFIID. Binding of TFIID to a promoter (with or without TATA element) is the initial step in preinitiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription.3 Publications

    GO - Molecular functioni

    1. chromatin binding Source: SGD
    2. DNA binding, bending Source: SGD
    3. protein binding Source: IntAct
    4. RNA polymerase II activating transcription factor binding Source: SGD
    5. RNA polymerase II core promoter sequence-specific DNA binding Source: SGD
    6. RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly Source: SGD
    7. RNA polymerase III regulatory region DNA binding Source: SGD
    8. RNA polymerase I transcription factor binding Source: SGD
    9. RNA polymerase I transcription factor recruiting transcription factor activity Source: SGD
    10. TFIIIB-type transcription factor activity Source: SGD

    GO - Biological processi

    1. regulation of transcription from RNA polymerase III promoter Source: GOC
    2. regulation of transcription from RNA polymerase I promoter Source: GOC
    3. RNA polymerase III transcriptional preinitiation complex assembly Source: SGD
    4. RNA polymerase II transcriptional preinitiation complex assembly Source: SGD
    5. transcription of nuclear large rRNA transcript from RNA polymerase I promoter Source: SGD
    6. transposon integration Source: SGD

    Keywords - Biological processi

    Transcription

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30309-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    TATA-box-binding protein
    Alternative name(s):
    TATA sequence-binding protein
    Short name:
    TBP
    TATA-binding factor
    TATA-box factor
    Transcription factor D
    Transcription initiation factor TFIID TBP subunit
    Gene namesi
    Name:SPT15
    Synonyms:BTF1, TBP1
    Ordered Locus Names:YER148W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome V

    Organism-specific databases

    SGDiS000000950. SPT15.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleoplasm Source: Reactome
    2. nucleus Source: SGD
    3. transcription factor TFIID complex Source: SGD
    4. transcription factor TFIIIB complex Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 240239TATA-box-binding proteinPRO_0000153990Add
    BLAST

    Proteomic databases

    MaxQBiP13393.
    PaxDbiP13393.
    PeptideAtlasiP13393.

    Expressioni

    Gene expression databases

    GenevestigatoriP13393.

    Interactioni

    Subunit structurei

    Binds DNA as monomer. The 1.2 MDa TFIID complex is composed of TATA binding protein (TBP) and the 14 TBP-associated factors. One copy of each TAF1, TAF2, TAF3, TAF7, TAF8, TAF11, TAF13, two copies of each TAF4, TAF5, TAF6, TAF9, TAF10, TAF12, and three copies of TAF14. Interacts with TFC8.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    BRF1P290562EBI-19129,EBI-19142
    KAP114P530673EBI-19129,EBI-9174
    MOT1P323339EBI-19129,EBI-11152
    RRN11Q047122EBI-19129,EBI-27790
    RVB1Q039403EBI-19129,EBI-30712
    RVB2Q124643EBI-19129,EBI-31814
    SPT8P389153EBI-19129,EBI-17964
    TOA2P327742EBI-19129,EBI-19323

    Protein-protein interaction databases

    BioGridi36897. 167 interactions.
    DIPiDIP-44N.
    IntActiP13393. 64 interactions.
    MINTiMINT-408820.
    STRINGi4932.YER148W.

    Structurei

    Secondary structure

    1
    240
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi66 – 7510
    Helixi82 – 887
    Beta strandi89 – 935
    Turni96 – 983
    Beta strandi100 – 1067
    Turni107 – 1104
    Beta strandi111 – 1155
    Beta strandi119 – 12810
    Helixi129 – 14618
    Beta strandi153 – 16513
    Helixi172 – 1787
    Turni179 – 1824
    Beta strandi183 – 1864
    Turni187 – 1893
    Beta strandi191 – 1966
    Beta strandi198 – 2003
    Beta strandi203 – 2064
    Beta strandi210 – 21910
    Helixi220 – 23617

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1NGMX-ray2.95A/E/I/M61-240[»]
    1NH2X-ray1.90A61-240[»]
    1RM1X-ray2.50A1-240[»]
    1TBANMR-B61-240[»]
    1TBPX-ray2.60A/B62-240[»]
    1YTBX-ray1.80A/B61-240[»]
    1YTFX-ray2.50A61-240[»]
    4B0AX-ray1.97A61-240[»]
    ProteinModelPortaliP13393.
    SMRiP13393. Positions 61-240.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP13393.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati67 – 143771Add
    BLAST
    Repeati157 – 234782Add
    BLAST

    Sequence similaritiesi

    Belongs to the TBP family.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG2101.
    GeneTreeiENSGT00410000025389.
    HOGENOMiHOG000105162.
    KOiK03120.
    OMAiQAFEMIY.
    OrthoDBiEOG77T1GJ.

    Family and domain databases

    Gene3Di3.30.310.10. 2 hits.
    HAMAPiMF_00408. TATA_bind_prot_arch.
    InterProiIPR012295. Beta2_adaptin/TBP_C_dom.
    IPR000814. TBP.
    [Graphical view]
    PANTHERiPTHR10126. PTHR10126. 1 hit.
    PfamiPF00352. TBP. 2 hits.
    [Graphical view]
    PRINTSiPR00686. TIFACTORIID.
    PROSITEiPS00351. TFIID. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P13393-1 [UniParc]FASTAAdd to Basket

    « Hide

    MADEERLKEF KEANKIVFDP NTRQVWENQN RDGTKPATTF QSEEDIKRAA    50
    PESEKDTSAT SGIVPTLQNI VATVTLGCRL DLKTVALHAR NAEYNPKRFA 100
    AVIMRIREPK TTALIFASGK MVVTGAKSED DSKLASRKYA RIIQKIGFAA 150
    KFTDFKIQNI VGSCDVKFPI RLEGLAFSHG TFSSYEPELF PGLIYRMVKP 200
    KIVLLIFVSG KIVLTGAKQR EEIYQAFEAI YPVLSEFRKM 240
    Length:240
    Mass (Da):27,003
    Last modified:January 23, 2007 - v3
    Checksum:i50365FFF1D3718CE
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti50 – 501A → T in AAA35146. (PubMed:2682626)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M27135 Genomic DNA. Translation: AAA35147.1.
    X16860 Genomic DNA. Translation: CAA34751.1.
    M26403 Genomic DNA. Translation: AAA35146.1.
    M29459 Genomic DNA. Translation: AAA34458.1.
    U18917 Genomic DNA. Translation: AAB64675.1.
    BK006939 Genomic DNA. Translation: DAA07809.1.
    PIRiA30366.
    RefSeqiNP_011075.3. NM_001179038.3.

    Genome annotation databases

    EnsemblFungiiYER148W; YER148W; YER148W.
    GeneIDi856891.
    KEGGisce:YER148W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M27135 Genomic DNA. Translation: AAA35147.1 .
    X16860 Genomic DNA. Translation: CAA34751.1 .
    M26403 Genomic DNA. Translation: AAA35146.1 .
    M29459 Genomic DNA. Translation: AAA34458.1 .
    U18917 Genomic DNA. Translation: AAB64675.1 .
    BK006939 Genomic DNA. Translation: DAA07809.1 .
    PIRi A30366.
    RefSeqi NP_011075.3. NM_001179038.3.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1NGM X-ray 2.95 A/E/I/M 61-240 [» ]
    1NH2 X-ray 1.90 A 61-240 [» ]
    1RM1 X-ray 2.50 A 1-240 [» ]
    1TBA NMR - B 61-240 [» ]
    1TBP X-ray 2.60 A/B 62-240 [» ]
    1YTB X-ray 1.80 A/B 61-240 [» ]
    1YTF X-ray 2.50 A 61-240 [» ]
    4B0A X-ray 1.97 A 61-240 [» ]
    ProteinModelPortali P13393.
    SMRi P13393. Positions 61-240.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36897. 167 interactions.
    DIPi DIP-44N.
    IntActi P13393. 64 interactions.
    MINTi MINT-408820.
    STRINGi 4932.YER148W.

    Proteomic databases

    MaxQBi P13393.
    PaxDbi P13393.
    PeptideAtlasi P13393.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YER148W ; YER148W ; YER148W .
    GeneIDi 856891.
    KEGGi sce:YER148W.

    Organism-specific databases

    SGDi S000000950. SPT15.

    Phylogenomic databases

    eggNOGi COG2101.
    GeneTreei ENSGT00410000025389.
    HOGENOMi HOG000105162.
    KOi K03120.
    OMAi QAFEMIY.
    OrthoDBi EOG77T1GJ.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30309-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P13393.
    NextBioi 983296.

    Gene expression databases

    Genevestigatori P13393.

    Family and domain databases

    Gene3Di 3.30.310.10. 2 hits.
    HAMAPi MF_00408. TATA_bind_prot_arch.
    InterProi IPR012295. Beta2_adaptin/TBP_C_dom.
    IPR000814. TBP.
    [Graphical view ]
    PANTHERi PTHR10126. PTHR10126. 1 hit.
    Pfami PF00352. TBP. 2 hits.
    [Graphical view ]
    PRINTSi PR00686. TIFACTORIID.
    PROSITEi PS00351. TFIID. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation of the gene encoding the yeast TATA binding protein TFIID: a gene identical to the SPT15 suppressor of Ty element insertions."
      Hahn S., Buratowski S., Sharp P.A., Guarente L.
      Cell 58:1173-1181(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "SPT15, the gene encoding the yeast TATA binding factor TFIID, is required for normal transcription initiation in vivo."
      Eisenmann D.M., Dollard C., Winston F.
      Cell 58:1183-1191(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Cloning and structure of a yeast gene encoding a general transcription initiation factor TFIID that binds to the TATA box."
      Horikoshi M., Wang C.K., Fujii H., Cromlish J.A., Weil P.A., Roeder R.G.
      Nature 341:299-303(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE OF 2-34; 122-162 AND 198-233, CLEAVAGE OF INITIATOR METHIONINE.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    5. "Cloning of the gene encoding the yeast protein BTF1Y, which can substitute for the human TATA box-binding factor."
      Cavallini B., Faus I., Matthes H., Chipoulet J.M., Winsor B., Egly J.-M., Chambon P.
      Proc. Natl. Acad. Sci. U.S.A. 86:9803-9807(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    7. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    8. "Cryptic initiation sequence revealed."
      Hoeijmakers J.H.J.
      Nature 343:417-418(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: REPEAT IN SEQUENCE.
    9. "Cryptic initiation sequence revealed."
      Nagai K.
      Nature 343:418-418(1990)
      Cited for: REPEAT IN SEQUENCE.
    10. "Molecular genetics of the RNA polymerase II general transcriptional machinery."
      Hampsey M.
      Microbiol. Mol. Biol. Rev. 62:465-503(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    11. "Molecular characterization of Saccharomyces cerevisiae TFIID."
      Sanders S.L., Garbett K.A., Weil P.A.
      Mol. Cell. Biol. 22:6000-6013(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TFIID STOICHIOMETRY.
    12. "Multi-protein complexes in eukaryotic gene transcription."
      Martinez E.
      Plant Mol. Biol. 50:925-947(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    13. "Structure of the tau60/Delta tau91 subcomplex of yeast transcription factor IIIC: insights into preinitiation complex assembly."
      Mylona A., Fernandez-Tornero C., Legrand P., Haupt M., Sentenac A., Acker J., Mueller C.W.
      Mol. Cell 24:221-232(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH TFC8.
    14. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    15. "Crystal structure of a yeast TBP/TATA-box complex."
      Kim Y., Geiger J.H., Hahn S., Sigler P.B.
      Nature 365:512-520(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 61-240.
    16. "Crystal structure of yeast TATA-binding protein and model for interaction with DNA."
      Chasman D.I., Flaherty K.M., Sharp P.A., Kornberg R.D.
      Proc. Natl. Acad. Sci. U.S.A. 90:8174-8178(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF 61-240.
    17. "Crystal structure of a yeast TFIIA/TBP/DNA complex."
      Tan S., Hunziker Y., Sargent D.F., Richmond T.J.
      Nature 381:127-134(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 61-240.
    18. "Solution structure of a TBP-TAF(II)230 complex: protein mimicry of the minor groove surface of the TATA box unwound by TBP."
      Liu D., Ishima R., Tong K.I., Bagby S., Kokubo T., Muhandiram D.R., Kay L.E., Nakatani Y., Ikura M.
      Cell 94:573-583(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 61-240.
    19. Cited for: 3D-STRUCTURE, ELECTRON MICROSCOPY OF TFIID.
    20. "Crystal structure of a transcription factor IIIB core interface ternary complex."
      Juo Z.S., Kassavetis G.A., Wang J., Geiduschek E.P., Sigler P.B.
      Nature 422:534-539(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.95 ANGSTROMS) OF 61-240 IN COMPLEX WITH BRF1.

    Entry informationi

    Entry nameiTBP_YEAST
    AccessioniPrimary (citable) accession number: P13393
    Secondary accession number(s): D3DM55
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 157 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome V
      Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

    External Data

    Dasty 3