P13389 (NEU1_SHEEP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Oxytocin-neurophysin 1
Short name=OT-NPI

Cleaved into the following 2 chains:

1. Oxytocin
   Alternative name(s):
   Ocytocin
2. Neurophysin 1

Gene names

Name:

OXT

Organism

Ovis aries (Sheep)

Taxonomic identifier

9940 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Caprinae › Ovis

Protein attributes

Sequence length	125 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Neurophysin 1 specifically binds oxytocin. Oxytocin causes contraction of the smooth muscle of the uterus and of the mammary gland.
Sequence similarities	Belongs to the vasopressin/oxytocin family.
Sequence caution	The sequence CAA34186.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Domain	Signal
Molecular function	Hormone
PTM	Amidation Cleavage on pair of basic residues Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: InterPro
Molecular function	neurohypophyseal hormone activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Ref.3
	Peptide	20 – 28	9	Oxytocin Ref.3		PRO_0000020505
	Chain	32 – 125	94	Neurophysin 1		PRO_0000020506
Amino acid modifications
	Modified residue	28	1	Glycine amide
	Disulfide bond	20 ↔ 25
	Disulfide bond	41 ↔ 85		By similarity
	Disulfide bond	44 ↔ 58		By similarity
	Disulfide bond	52 ↔ 75		By similarity
	Disulfide bond	59 ↔ 65		By similarity
	Disulfide bond	92 ↔ 104		By similarity
	Disulfide bond	98 ↔ 116		By similarity
	Disulfide bond	105 ↔ 110		By similarity
Experimental info
	Sequence conflict	48	1	G → A in CAA34184. Ref.1
	Sequence conflict	48	1	G → A in CAA34186. Ref.1
	Sequence conflict	110	1	C → S in CAA34184. Ref.1
	Sequence conflict	110	1	C → S in CAA34186. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P13389 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: 3B4C05141422E432	FASTA	125	12,635
	10 20 30 40 50 60 MAGSSLACCL LGLLALTSAC YIQNCPLGGK RAVLDLDVRT CLPCGPGGKG RCFGPSICCG 70 80 90 100 110 120 DELGCFVGTA EALRCREENY LPSPCQSGQK PCGSGGRCAA AGICCSPDGC HADPACDPEA AFSQH

References

[1]	"Nucleotide sequence of a full length cDNA clone encoding the oxytocin-neurophysin I precursor isolated from the ovine corpus luteum." Jones D.S.C., Flint A.P.F. Nucleic Acids Res. 17:7990-7990(1989) [PubMed: 2798140] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Corpus luteum.
[2]	"Structure and ovarian expression of the oxytocin gene in sheep." Ivell R., Hunt N., Abend N., Brackman B., Nollmeyer D., Lamsa J.C., McCracken J.A. Reprod. Fertil. Dev. 2:703-711(1990) [PubMed: 2095591] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Ovary.
[3]	"Purification and structure of sheep oxytocin and vasopressin." Acher R., Chauvet J., Lenci M.T. C. R. Hebd. Seances Acad. Sci., D, Sci. Nat. 248:1435-1438(1959) Cited for: PROTEIN SEQUENCE OF 20-28.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X16052 mRNA. Translation: CAA34184.1. X16052 mRNA. Translation: CAA34185.1. Sequence problems. X16052 mRNA. Translation: CAA34186.1. Different initiation. X55131 Genomic DNA. Translation: CAA38924.1.
PIR	NFSH1. S06086.
RefSeq	NP_001009801.1. NM_001009801.1.
UniGene	Oar.444.
3D structure databases
ProteinModelPortal	P13389.
SMR	P13389. Positions 32-123.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	443390.
Organism-specific databases
CTD	5020.
Phylogenomic databases
HOVERGEN	HBG004462.
Family and domain databases
InterPro	IPR000981. Neurhyp_horm. IPR022423. Neurohypophysial_hormone_CS. [Graphical view]
Gene3D	G3DSA:2.60.9.10. Neurhyp_horm. 1 hit.
PANTHER	PTHR11681. PTHR11681. 1 hit.
Pfam	PF00220. Hormone_4. 1 hit. PF00184. Hormone_5. 1 hit. [Graphical view]
PIRSF	PIRSF001815. Nonapeptide_hormone_precursor. 1 hit.
PRINTS	PR00831. NEUROPHYSIN.
ProDom	PD001676. Neurhyp_horm. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00003. NH. 1 hit. [Graphical view]
SUPFAM	SSF49606. Neurhyp_horm. 1 hit.
PROSITE	PS00264. NEUROHYPOPHYS_HORM. 1 hit. [Graphical view]
ProtoNet	Search...
Other
PMAP-CutDB	P13389.

Entry information

Entry name

NEU1_SHEEP

Accession

Primary (citable) accession number: P13389
Secondary accession number(s): P01188

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	February 1, 1996
Last modified:	October 19, 2011
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program