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P13385 (TDGF1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 133. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Teratocarcinoma-derived growth factor 1
Alternative name(s):
Cripto-1 growth factor
Short name=CRGF
Epidermal growth factor-like cripto protein CR1
Gene names
Name:TDGF1
Synonyms:CRIPTO
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length188 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm. Ref.7

Subunit structure

Interacts with the activin type-1 receptor ACVR1B. Ref.7

Subcellular location

Cell membrane; Lipid-anchorGPI-anchor Ref.9.

Tissue specificity

Preferentially expressed in gastric and colorectal carcinomas than in their normal counterparts. Expressed in breast and lung. Ref.10

Sequence similarities

Contains 1 EGF-like domain.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   Coding sequence diversityPolymorphism
   DomainEGF-like domain
Signal
   Molecular functionGrowth factor
   PTMDisulfide bond
Glycoprotein
GPI-anchor
Lipoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processBMP signaling pathway

Inferred from electronic annotation. Source: Ensembl

activation of MAPK activity

Inferred from direct assay PubMed 9013573. Source: UniProtKB

anterior/posterior axis specification, embryo

Inferred from sequence or structural similarity. Source: UniProtKB

canonical Wnt signaling pathway

Inferred from electronic annotation. Source: Ensembl

cardiac muscle cell differentiation

Inferred from electronic annotation. Source: Ensembl

cell differentiation

Traceable author statement PubMed 11857442. Source: UniProtKB

cell migration involved in sprouting angiogenesis

Inferred from mutant phenotype PubMed 17720976. Source: UniProtKB

cellular response to epidermal growth factor stimulus

Inferred from direct assay PubMed 17720976. Source: UniProtKB

cellular response to fibroblast growth factor stimulus

Inferred from direct assay PubMed 17720976. Source: UniProtKB

cellular response to hepatocyte growth factor stimulus

Inferred from direct assay PubMed 17720976. Source: UniProtKB

cellular response to interferon-gamma

Inferred from direct assay PubMed 17720976. Source: UniProtKB

cellular response to interleukin-6

Inferred from direct assay PubMed 17720976. Source: UniProtKB

cellular response to tumor necrosis factor

Inferred from direct assay PubMed 17720976. Source: UniProtKB

embryo development

Traceable author statement PubMed 11857442. Source: UniProtKB

gastrulation

Inferred from electronic annotation. Source: Ensembl

heart development

Inferred from direct assay PubMed 9576836. Source: UniProtKB

in utero embryonic development

Inferred from electronic annotation. Source: Ensembl

mammary gland development

Traceable author statement PubMed 11857442. Source: UniProtKB

morphogenesis of a branching structure

Traceable author statement PubMed 10070255. Source: UniProtKB

negative regulation of apoptotic process

Inferred from direct assay PubMed 10493495. Source: UniProtKB

negative regulation of transforming growth factor beta receptor signaling pathway

Inferred from electronic annotation. Source: Ensembl

peptidyl-serine phosphorylation

Inferred from direct assay PubMed 10493495. Source: UniProtKB

positive regulation of cell migration

Inferred from direct assay PubMed 12649175. Source: UniProtKB

positive regulation of cell proliferation

Traceable author statement PubMed 11857442. Source: UniProtKB

positive regulation of cell-matrix adhesion

Inferred from electronic annotation. Source: Ensembl

positive regulation of endothelial cell migration

Inferred from direct assay PubMed 17720976. Source: UniProtKB

positive regulation of fibroblast proliferation

Inferred from electronic annotation. Source: Ensembl

positive regulation of peptidyl-tyrosine phosphorylation

Inferred from direct assay PubMed 10493495. Source: UniProtKB

positive regulation of transcription from RNA polymerase II promoter

Inferred from electronic annotation. Source: Ensembl

regulation of signal transduction

Inferred from direct assay PubMed 12649175. Source: UniProtKB

vasculogenesis

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentGolgi apparatus

Inferred from electronic annotation. Source: Ensembl

anchored component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

apical plasma membrane

Inferred from direct assay PubMed 17720976. Source: UniProtKB

cell surface

Inferred from direct assay PubMed 12925698. Source: UniProtKB

extracellular space

Inferred from direct assay PubMed 17720976. Source: UniProtKB

extrinsic component of plasma membrane

Inferred from sequence or structural similarity. Source: UniProtKB

membrane raft

Inferred from direct assay PubMed 17720976. Source: UniProtKB

nucleus

Inferred from electronic annotation. Source: Ensembl

perinuclear region of cytoplasm

Inferred from electronic annotation. Source: Ensembl

   Molecular_functiongrowth factor activity

Inferred from direct assay PubMed 12925698. Source: UniProtKB

receptor binding

Inferred from direct assay Ref.7. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3030 Ref.6
Chain31 – 150120Teratocarcinoma-derived growth factor 1
PRO_0000007503
Propeptide151 – 18838Removed in mature form
PRO_0000395410

Regions

Domain78 – 10730EGF-like

Amino acid modifications

Lipidation1501GPI-anchor amidated aspartate Ref.9
Glycosylation791N-linked (GlcNAc...) Potential
Disulfide bond82 ↔ 89 By similarity
Disulfide bond83 ↔ 95 By similarity
Disulfide bond97 ↔ 106 By similarity
Disulfide bond115 ↔ 133 Ref.8
Disulfide bond128 ↔ 149 Ref.8
Disulfide bond131 ↔ 140 Ref.8

Natural variations

Natural variant221V → A. Ref.4 Ref.5
Corresponds to variant rs11130097 [ dbSNP | Ensembl ].
VAR_024262
Natural variant431Y → D.
Corresponds to variant rs2293025 [ dbSNP | Ensembl ].
VAR_021903
Natural variant1111R → G.
Corresponds to variant rs34501971 [ dbSNP | Ensembl ].
VAR_048975

Experimental info

Mutagenesis1881Y → YVVVVV: Alters the localization and decreases the biological activity. Ref.9

Sequences

Sequence LengthMass (Da)Tools
P13385 [UniParc].

Last modified January 1, 1990. Version 1.
Checksum: AEE8727D0F27D886

FASTA18821,169
        10         20         30         40         50         60 
MDCRKMARFS YSVIWIMAIS KVFELGLVAG LGHQEFARPS RGYLAFRDDS IWPQEEPAIR 

        70         80         90        100        110        120 
PRSSQRVPPM GIQHSKELNR TCCLNGGTCM LGSFCACPPS FYGRNCEHDV RKENCGSVPH 

       130        140        150        160        170        180 
DTWLPKKCSL CKCWHGQLRC FPQAFLPGCD GLVMDEHLVA SRTPELPPSA RTTTFMLVGI 


CLSIQSYY 

« Hide

References

« Hide 'large scale' references
[1]"Molecular characterization of a gene of the 'EGF family' expressed in undifferentiated human NTERA2 teratocarcinoma cells."
Ciccodicola A., Dono R., Obici S., Zollo M., Persico M.G.
EMBO J. 8:1987-1991(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Isolation and characterization of the CRIPTO autosomal gene and its X-linked related sequence."
Dono R., Montuori N., Rocchi M., de Ponti-Zilli L., Ciccodicola A., Persico M.G.
Am. J. Hum. Genet. 49:555-565(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The DNA sequence, annotation and analysis of human chromosome 3."
Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J. expand/collapse author list , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.
Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT ALA-22.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ALA-22.
Tissue: Brain and Kidney.
[6]"Signal peptide prediction based on analysis of experimentally verified cleavage sites."
Zhang Z., Henzel W.J.
Protein Sci. 13:2819-2824(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 31-45.
[7]"Cripto-1 activates nodal- and ALK4-dependent and -independent signaling pathways in mammary epithelial Cells."
Bianco C., Adkins H.B., Wechselberger C., Seno M., Normanno N., De Luca A., Sun Y., Khan N., Kenney N., Ebert A., Williams K.P., Sanicola M., Salomon D.S.
Mol. Cell. Biol. 22:2586-2597(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ACVR1B, FUNCTION.
[8]"The CRIPTO/FRL-1/CRYPTIC (CFC) domain of human Cripto."
Foley S.F., Van Vlijmen H.W., Boynton R.E., Adkins H.B., Cheung A.E., Singh J., Sanicola M., Young C.N., Wen D.
Eur. J. Biochem. 270:3610-3618(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: DISULFIDE BONDS.
[9]"Characterization of the glycosylphosphatidylinositol-anchor signal sequence of human Cryptic with a hydrophilic extension."
Watanabe K., Nagaoka T., Strizzi L., Mancino M., Gonzales M., Bianco C., Salomon D.S.
Biochim. Biophys. Acta 1778:2671-2681(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, GPI-ANCHOR, MUTAGENESIS OF TYR-188.
[10]"CRIPTO3, a presumed pseudogene, is expressed in cancer."
Sun C., Orozco O., Olson D.L., Choi E., Garber E., Tizard R., Szak S., Sanicola M., Carulli J.P.
Biochem. Biophys. Res. Commun. 377:215-220(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X14253 mRNA. Translation: CAA32467.1.
M96955 Genomic DNA. Translation: AAA61134.1.
AC104304 Genomic DNA. No translation available.
CH471055 Genomic DNA. Translation: EAW64773.1.
BC022393 mRNA. Translation: AAH22393.1.
BC067844 mRNA. Translation: AAH67844.1.
PIRA30362. B39787.
RefSeqNP_001167607.1. NM_001174136.1.
NP_003203.1. NM_003212.3.
UniGeneHs.385870.

3D structure databases

ProteinModelPortalP13385.
SMRP13385. Positions 73-150.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid112856. 9 interactions.
IntActP13385. 9 interactions.
MINTMINT-1386411.
STRING9606.ENSP00000296145.

Polymorphism databases

DMDM117473.

Proteomic databases

PaxDbP13385.
PRIDEP13385.

Protocols and materials databases

DNASU6997.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000296145; ENSP00000296145; ENSG00000241186.
GeneID6997.
KEGGhsa:6997.
UCSCuc003cpv.3. human.

Organism-specific databases

CTD6997.
GeneCardsGC03P046616.
HGNCHGNC:11701. TDGF1.
MIM187395. gene+phenotype.
neXtProtNX_P13385.
Orphanet93925. Alobar holoprosencephaly.
93924. Lobar holoprosencephaly.
280200. Microform holoprosencephaly.
93926. Midline interhemispheric variant of holoprosencephaly.
220386. Semilobar holoprosencephaly.
280195. Septopreoptic holoprosencephaly.
PharmGKBPA36420.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG320031.
HOGENOMHOG000145523.
HOVERGENHBG054705.
InParanoidP13385.
OMANCEHDVR.
OrthoDBEOG76X61G.
PhylomeDBP13385.
TreeFamTF333187.

Enzyme and pathway databases

ReactomeREACT_111045. Developmental Biology.

Gene expression databases

ArrayExpressP13385.
BgeeP13385.
CleanExHS_TDGF1.
GenevestigatorP13385.

Family and domain databases

InterProIPR017047. Cripto_growth_factor.
IPR019011. Cryptic/Cripto_CFC-dom.
IPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
[Graphical view]
PfamPF09443. CFC. 1 hit.
[Graphical view]
PIRSFPIRSF036301. Cripto_growth_factor. 1 hit.
SMARTSM00181. EGF. 1 hit.
[Graphical view]
PROSITEPS00022. EGF_1. 1 hit.
PS50026. EGF_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSTDGF1. human.
GeneWikiTeratocarcinoma-derived_growth_factor_1.
GenomeRNAi6997.
NextBio27331.
PROP13385.
SOURCESearch...

Entry information

Entry nameTDGF1_HUMAN
AccessionPrimary (citable) accession number: P13385
Secondary accession number(s): Q8TCC1
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: April 16, 2014
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 3

Human chromosome 3: entries, gene names and cross-references to MIM