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Protein

Teratocarcinoma-derived growth factor 1

Gene

TDGF1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

GPI-anchored cell membrane protein involved in Nodal signaling. Cell-associated TDGF1 acts as a Nodal coreceptor in cis. Shedding of TDGF1 by TMEM8A modulates Nodal signaling by allowing soluble TDGF1 to act as a Nodal coreceptor on other cells (PubMed:27881714). Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm (PubMed:11909953).2 Publications

GO - Molecular functioni

  • growth factor activity Source: UniProtKB
  • receptor binding Source: UniProtKB

GO - Biological processi

  • activation of MAPK activity Source: UniProtKB
  • anterior/posterior axis specification, embryo Source: UniProtKB
  • cell differentiation Source: UniProtKB
  • cell migration involved in sprouting angiogenesis Source: UniProtKB
  • cellular response to epidermal growth factor stimulus Source: UniProtKB
  • cellular response to fibroblast growth factor stimulus Source: UniProtKB
  • cellular response to hepatocyte growth factor stimulus Source: UniProtKB
  • cellular response to interferon-gamma Source: UniProtKB
  • cellular response to interleukin-6 Source: UniProtKB
  • cellular response to tumor necrosis factor Source: UniProtKB
  • embryo development ending in birth or egg hatching Source: UniProtKB
  • heart development Source: UniProtKB
  • mammary gland development Source: UniProtKB
  • morphogenesis of a branching structure Source: UniProtKB
  • negative regulation of apoptotic process Source: UniProtKB
  • peptidyl-serine phosphorylation Source: UniProtKB
  • positive regulation of cell migration Source: UniProtKB
  • positive regulation of cell proliferation Source: MGI
  • positive regulation of endothelial cell migration Source: UniProtKB
  • positive regulation of peptidyl-tyrosine phosphorylation Source: UniProtKB
  • regulation of signal transduction Source: UniProtKB
  • somatic stem cell population maintenance Source: Reactome

Keywordsi

Molecular functionGrowth factor

Enzyme and pathway databases

ReactomeiR-HSA-1181150 Signaling by NODAL
R-HSA-1433617 Regulation of signaling by NODAL
R-HSA-2892247 POU5F1 (OCT4), SOX2, NANOG activate genes related to proliferation
SIGNORiP13385

Names & Taxonomyi

Protein namesi
Recommended name:
Teratocarcinoma-derived growth factor 1
Alternative name(s):
Cripto-1 growth factor
Short name:
CRGF
Epidermal growth factor-like cripto protein CR1
Gene namesi
Name:TDGF1
Synonyms:CRIPTO
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

EuPathDBiHostDB:ENSG00000241186.7
HGNCiHGNC:11701 TDGF1
MIMi187395 gene+phenotype
neXtProtiNX_P13385

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane, Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi188Y → YVVVVV: Alters the localization and decreases the biological activity. 1 Publication1

Organism-specific databases

DisGeNETi6997
MalaCardsiTDGF1
MIMi187395 gene+phenotype
OpenTargetsiENSG00000241186
Orphaneti93925 Alobar holoprosencephaly
93924 Lobar holoprosencephaly
280200 Microform holoprosencephaly
93926 Midline interhemispheric variant of holoprosencephaly
220386 Semilobar holoprosencephaly
280195 Septopreoptic holoprosencephaly
PharmGKBiPA36420

Polymorphism and mutation databases

BioMutaiTDGF1
DMDMi117473

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 301 PublicationAdd BLAST30
ChainiPRO_000000750331 – 150Teratocarcinoma-derived growth factor 1Add BLAST120
PropeptideiPRO_0000395410151 – 188Removed in mature formAdd BLAST38

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi79N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi82 ↔ 89PROSITE-ProRule annotation
Disulfide bondi83 ↔ 95PROSITE-ProRule annotation
Disulfide bondi97 ↔ 106PROSITE-ProRule annotation
Disulfide bondi115 ↔ 133PROSITE-ProRule annotation1 Publication
Disulfide bondi128 ↔ 149PROSITE-ProRule annotation1 Publication
Disulfide bondi131 ↔ 140PROSITE-ProRule annotation1 Publication
Lipidationi150GPI-anchor amidated aspartate1 Publication1

Post-translational modificationi

The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, it is processed by GPI processing phospholipase A2 (TMEM8A), removing an acyl-chain at the sn-2 position of GPI and releasing TDGF1 as a lysophosphatidylinositol-bearing form, which is further cleaved by phospholipase D (GPLD1) into a soluble form.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiP13385
PeptideAtlasiP13385
PRIDEiP13385

Expressioni

Tissue specificityi

Preferentially expressed in gastric and colorectal carcinomas than in their normal counterparts. Expressed in breast and lung.1 Publication

Gene expression databases

BgeeiENSG00000241186
CleanExiHS_TDGF1
ExpressionAtlasiP13385 baseline and differential
GenevisibleiP13385 HS

Interactioni

Subunit structurei

Interacts with the activin type-1 receptor ACVR1B.1 Publication

GO - Molecular functioni

  • growth factor activity Source: UniProtKB
  • receptor binding Source: UniProtKB

Protein-protein interaction databases

BioGridi11285631 interactors.
IntActiP13385 9 interactors.
MINTiP13385
STRINGi9606.ENSP00000296145

Structurei

3D structure databases

ProteinModelPortaliP13385
SMRiP13385
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini78 – 107EGF-likePROSITE-ProRule annotationAdd BLAST30

Sequence similaritiesi

Keywords - Domaini

EGF-like domain, Signal

Phylogenomic databases

eggNOGiKOG1217 Eukaryota
ENOG410XP6K LUCA
GeneTreeiENSGT00530000063804
HOGENOMiHOG000145523
HOVERGENiHBG054705
InParanoidiP13385
OMAiNCEHDVR
OrthoDBiEOG091G0RA4
PhylomeDBiP13385
TreeFamiTF333187

Family and domain databases

InterProiView protein in InterPro
IPR017047 Cripto_growth_factor
IPR019011 Cryptic/Cripto_CFC-dom
IPR013032 EGF-like_CS
IPR000742 EGF-like_dom
PANTHERiPTHR14949:SF26 PTHR14949:SF26, 1 hit
PfamiView protein in Pfam
PF09443 CFC, 1 hit
PIRSFiPIRSF036301 Cripto_growth_factor, 1 hit
PROSITEiView protein in PROSITE
PS00022 EGF_1, 1 hit
PS50026 EGF_3, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P13385-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDCRKMARFS YSVIWIMAIS KVFELGLVAG LGHQEFARPS RGYLAFRDDS
60 70 80 90 100
IWPQEEPAIR PRSSQRVPPM GIQHSKELNR TCCLNGGTCM LGSFCACPPS
110 120 130 140 150
FYGRNCEHDV RKENCGSVPH DTWLPKKCSL CKCWHGQLRC FPQAFLPGCD
160 170 180
GLVMDEHLVA SRTPELPPSA RTTTFMLVGI CLSIQSYY
Length:188
Mass (Da):21,169
Last modified:January 1, 1990 - v1
Checksum:iAEE8727D0F27D886
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_02426222V → A2 PublicationsCorresponds to variant dbSNP:rs11130097Ensembl.1
Natural variantiVAR_02190343Y → D. Corresponds to variant dbSNP:rs2293025Ensembl.1
Natural variantiVAR_048975111R → G. Corresponds to variant dbSNP:rs34501971Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14253 mRNA Translation: CAA32467.1
M96955 Genomic DNA Translation: AAA61134.1
AC104304 Genomic DNA No translation available.
CH471055 Genomic DNA Translation: EAW64773.1
BC022393 mRNA Translation: AAH22393.1
BC067844 mRNA Translation: AAH67844.1
CCDSiCCDS2742.1
PIRiB39787 A30362
RefSeqiNP_001167607.1, NM_001174136.1
NP_003203.1, NM_003212.3
UniGeneiHs.385870

Genome annotation databases

EnsembliENST00000296145; ENSP00000296145; ENSG00000241186
GeneIDi6997
KEGGihsa:6997
UCSCiuc003cpv.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiTDGF1_HUMAN
AccessioniPrimary (citable) accession number: P13385
Secondary accession number(s): Q8TCC1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: March 28, 2018
This is version 165 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome