P13384 (IBP2_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Insulin-like growth factor-binding protein 2
Short name=IBP-2
Short name=IGF-binding protein 2
Short name=IGFBP-2

Gene names

Name:

IGFBP2

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	317 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Inhibits IGF-mediated growth and developmental rates By similarity. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
Subunit structure	Binds IGF2 more than IGF1 By similarity.
Subcellular location	Secreted.
Domain	The C-terminus is required for IGF-binding and growth inhibition By similarity.
Post-translational modification	O-glycosylated By similarity.
Sequence similarities	Contains 1 IGFBP N-terminal domain. Contains 1 thyroglobulin type-1 domain.

Ontologies

Keywords
Biological process	Growth regulation
Cellular component	Secreted
Domain	Signal
Ligand	Growth factor binding
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	regulation of cell growth Inferred from electronic annotation. Source: InterPro regulation of insulin-like growth factor receptor signaling pathway Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	extracellular region Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	insulin-like growth factor I binding Inferred from sequence or structural similarity. Source: UniProtKB insulin-like growth factor II binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 33	33	Ref.3
	Chain	34 – 317	284	Insulin-like growth factor-binding protein 2		PRO_0000014369
Regions
	Domain	35 – 126	92	IGFBP N-terminal
	Domain	216 – 298	83	Thyroglobulin type-1
	Motif	293 – 295	3	Cell attachment site Potential
Amino acid modifications
	Disulfide bond	219 ↔ 253		By similarity
	Disulfide bond	264 ↔ 275		By similarity
	Disulfide bond	277 ↔ 298		By similarity
Experimental info
	Sequence conflict	32 – 33	2	RA → AS Ref.2
	Sequence conflict	46	1	S → C AA sequence Ref.3
	Sequence conflict	60 – 61	2	PA → AP Ref.2
	Sequence conflict	82	1	C → F AA sequence Ref.3
	Sequence conflict	114	1	R → H Ref.2
	Sequence conflict	128	1	D → S Ref.2
	Sequence conflict	134	1	S → T Ref.2
	Sequence conflict	313	1	T → N Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P13384 [UniParc]. Last modified November 23, 2004. Version 2. Checksum: 2B552BC61CA7E9CD	FASTA	317	34,015
	10 20 30 40 50 60 MQPRLGGPAL LLLPPLLLLL LLGAGGGDCG ARAEVLFRCP PCTPESLAAC KPPPGAAAGP 70 80 90 100 110 120 AGDARVPCEL VREPGCGCCS VCARLEGERC GVYTPRCGQG LRCYPNPGSE LPLRALVHGE 130 140 150 160 170 180 GTCEKHGDAE YSASPEQVAD NGEEHSEGGL VENHVDGNVN LMGGGGGAGR KPLKSGMKEL 190 200 210 220 230 240 AVFREKVTEQ HRQMGKGGKH HLGLEEPKKL RPPPARTPCQ QELDQVLERI STMRLPDERG 250 260 270 280 290 300 PLEHLYSLHI PNCDKHGLYN LKQCKMSLNG QRGECWCVNP NTGKLIQGAP TIRGDPECHL 310 FYNEQQGARG VHTQRMQ

References

[1]	"Cloning and sequence determination of bovine insulin-like growth factor binding protein-2 (IGFBP-2): comparison of its structural and functional properties with IGFBP-1." Bourner M.J., Busby W.H. Jr., Siegel N.R., Krivi G.G., McCusker R.H., Clemmons D.R. J. Cell. Biochem. 48:215-226(1992) [PubMed: 1377702] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
[2]	"Characterization and cloning of a bovine insulin-like growth factor-binding protein." Upton F.Z., Szabo L., Wallace J.C., Ballard F.J. J. Mol. Endocrinol. 5:77-84(1990) [PubMed: 1697752] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 8-317.
[3]	"The bovine insulin-like growth factor (IGF) binding protein purified from conditioned medium requires the N-terminal tripeptide in IGF-1 for binding." Szabo L., Mottershead D.G., Ballard F.J., Wallace J.C. Biochem. Biophys. Res. Commun. 151:207-214(1988) [PubMed: 2450535] [Abstract] Cited for: PROTEIN SEQUENCE OF 34-86.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF074854 mRNA. Translation: AAD04862.1.
IPI	IPI00705697.
PIR	A60967.
RefSeq	NP_776980.1. NM_174555.1.
UniGene	Bt.149.
3D structure databases
ProteinModelPortal	P13384.
SMR	P13384. Positions 37-125, 212-317.
ModBase	Search...
Protein-protein interaction databases
STRING	P13384.
Protein family/group databases
MEROPS	I31.953.
Proteomic databases
PRIDE	P13384.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	282260.
KEGG	bta:282260.
Organism-specific databases
CTD	3485.
Phylogenomic databases
GeneTree	ENSGT00550000074457.
HOVERGEN	HBG002631.
InParanoid	P13384.
OrthoDB	EOG4TTGJD.
Family and domain databases
InterPro	IPR009030. Growth_fac_rcpt. IPR012210. IGFBP-2. IPR000867. IGFBP-like. IPR009168. IGFBP1-6. IPR022321. IGFBP_1-6_chordata. IPR017891. Insulin_GF-bd_Cys-rich_CS. IPR000716. Thyroglobulin_1. [Graphical view]
Gene3D	G3DSA:4.10.800.10. Thyroglobulin_1. 1 hit.
PANTHER	PTHR11551:SF5. IGFBP-2. 1 hit. PTHR11551. IGFBP1-6. 1 hit.
Pfam	PF00219. IGFBP. 1 hit. PF00086. Thyroglobulin_1. 1 hit. [Graphical view]
PRINTS	PR01976. IGFBPFAMILY. PR01978. IGFBPFAMILY2.
SMART	SM00121. IB. 1 hit. SM00211. TY. 1 hit. [Graphical view]
SUPFAM	SSF57184. Grow_fac_recept. 1 hit. SSF57610. Thyroglobulin_1. 1 hit.
PROSITE	PS00222. IGFBP_N_1. 1 hit. PS51323. IGFBP_N_2. 1 hit. PS00484. THYROGLOBULIN_1_1. 1 hit. PS51162. THYROGLOBULIN_1_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IBP2_BOVIN

Accession

Primary (citable) accession number: P13384
Secondary accession number(s): O97599

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	November 23, 2004
Last modified:	November 16, 2011
This is version 89 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program