Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Nucleolin

Gene

Ncl

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats (By similarity).By similarity

GO - Molecular functioni

  • calcium ion binding Source: RGD
  • ErbB-4 class receptor binding Source: RGD
  • histone binding Source: RGD
  • laminin binding Source: RGD
  • nucleotide binding Source: InterPro
  • receptor binding Source: RGD
  • RNA binding Source: UniProtKB
  • rRNA primary transcript binding Source: RGD
  • selenocysteine insertion sequence binding Source: RGD
  • sequence-specific DNA binding Source: RGD
  • single-stranded DNA binding Source: RGD
  • telomeric DNA binding Source: UniProtKB

GO - Biological processi

  • cellular response to lipopolysaccharide Source: RGD
  • endocytosis Source: RGD
  • liver regeneration Source: RGD
  • negative regulation of apoptotic process Source: RGD
  • positive regulation of interleukin-6 secretion Source: RGD
  • positive regulation of tumor necrosis factor production Source: RGD
  • regulation of rRNA processing Source: RGD
  • spermatogenesis Source: RGD
Complete GO annotation...

Keywords - Ligandi

DNA-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleolin
Alternative name(s):
Protein C23
Gene namesi
Name:Ncl
Synonyms:Nuc
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3153. Ncl.

Subcellular locationi

  • Nucleusnucleolus
  • Cytoplasm By similarity

  • Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs.By similarity

GO - Cellular componenti

  • cell surface Source: RGD
  • cytoplasm Source: RGD
  • dense fibrillar component Source: RGD
  • fibrillar center Source: RGD
  • intracellular ribonucleoprotein complex Source: UniProtKB
  • nucleolus Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 713712NucleolinPRO_0000081694Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei9 – 91N6-acetyllysineBy similarity
Modified residuei15 – 151N6-acetyllysineBy similarity
Modified residuei16 – 161N6-acetyllysineBy similarity
Modified residuei28 – 281PhosphoserineCombined sources
Modified residuei34 – 341PhosphoserineBy similarity
Modified residuei40 – 401PhosphoserineCombined sources
Modified residuei41 – 411PhosphoserineCombined sources
Modified residuei67 – 671PhosphoserineBy similarity
Modified residuei69 – 691PhosphothreonineBy similarity
Modified residuei76 – 761PhosphothreonineBy similarity
Modified residuei84 – 841PhosphothreonineBy similarity
Modified residuei92 – 921PhosphothreonineBy similarity
Modified residuei96 – 961N6-acetyllysineBy similarity
Modified residuei99 – 991PhosphothreonineBy similarity
Modified residuei102 – 1021N6-acetyllysineBy similarity
Modified residuei106 – 1061PhosphothreonineBy similarity
Modified residuei109 – 1091N6-acetyllysineBy similarity
Modified residuei116 – 1161N6-acetyllysineBy similarity
Modified residuei121 – 1211PhosphothreonineBy similarity
Modified residuei124 – 1241N6-acetyllysineBy similarity
Modified residuei145 – 1451PhosphoserineCombined sources
Modified residuei157 – 1571PhosphoserineCombined sources
Modified residuei187 – 1871PhosphoserineCombined sources
Modified residuei213 – 2131PhosphoserineCombined sources
Modified residuei221 – 2211PhosphothreonineBy similarity
Modified residuei305 – 3051PhosphoserineCombined sources
Modified residuei322 – 3221N6-acetyllysineBy similarity
Modified residuei352 – 3521N6-acetyllysineBy similarity
Modified residuei381 – 3811N6-acetyllysineBy similarity
Modified residuei402 – 4021N6-acetyllysineBy similarity
Modified residuei405 – 4051PhosphoserineCombined sources
Modified residuei448 – 4481N6-acetyllysineBy similarity
Modified residuei462 – 4621PhosphoserineBy similarity
Modified residuei471 – 4711N6-acetyllysineBy similarity
Modified residuei480 – 4801N6-acetyllysineBy similarity
Modified residuei516 – 5161N6-acetyllysineBy similarity
Modified residuei524 – 5241N6-acetyllysineBy similarity
Modified residuei566 – 5661PhosphoserineCombined sources
Modified residuei575 – 5751N6-acetyllysineBy similarity
Modified residuei580 – 5801N6-acetyllysineBy similarity
Modified residuei583 – 5831PhosphoserineCombined sources
Modified residuei594 – 5941PhosphoserineBy similarity
Modified residuei622 – 6221PhosphoserineCombined sources
Modified residuei649 – 6491N6-acetyllysineBy similarity
Modified residuei659 – 6591Asymmetric dimethylarginineBy similarity
Modified residuei663 – 6631Asymmetric dimethylarginineBy similarity
Modified residuei669 – 6691Asymmetric dimethylarginineBy similarity
Modified residuei673 – 6731Asymmetric dimethylarginineBy similarity
Modified residuei676 – 6761Asymmetric dimethylarginineBy similarity

Post-translational modificationi

Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group (By similarity).By similarity

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

PaxDbiP13383.
PRIDEiP13383.

PTM databases

iPTMnetiP13383.
PhosphoSiteiP13383.

Interactioni

Subunit structurei

Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs. Interacts with APTX and NSUN2. Component of the SWAP complex that consists of NPM1, NCL/nucleolin, PARP1 and SWAP70. Component of a complex which is at least composed of HTATSF1/Tat-SF1, the P-TEFb complex components CDK9 and CCNT1, RNA polymerase II, SUPT5H, and NCL/nucleolin. Interacts (via RRM1 and C-terminal RRM4/Arg/Gly-rich domains) with TERT; the interaction is important for nucleolar localization of TERT. Interacts with ERBB4. Interacts with ERBB4. Interacts with GZF1; this interaction is important for nucleolar localization of GZF1. Interacts with NVL and C1QBP. Interacts with AICDA. Interacts (via N-terminus domain) with SETX. Interacts with WDR46 (By similarity).By similarity

GO - Molecular functioni

  • ErbB-4 class receptor binding Source: RGD
  • histone binding Source: RGD
  • laminin binding Source: RGD
  • receptor binding Source: RGD

Protein-protein interaction databases

IntActiP13383. 2 interactions.
STRINGi10116.ENSRNOP00000024712.

Structurei

3D structure databases

ProteinModelPortaliP13383.
SMRiP13383. Positions 302-472, 487-651.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati58 – 6581
Repeati75 – 8282
Repeati83 – 9083
Repeati91 – 9884
Repeati99 – 10465; truncated
Repeati105 – 11286
Repeati120 – 12787
Repeati128 – 13588
Domaini311 – 38777RRM 1PROSITE-ProRule annotationAdd
BLAST
Domaini397 – 47074RRM 2PROSITE-ProRule annotationAdd
BLAST
Domaini489 – 56375RRM 3PROSITE-ProRule annotationAdd
BLAST
Domaini575 – 65076RRM 4PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni58 – 135788 X 8 AA tandem repeats of X-T-P-X-K-K-X-XAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi143 – 16826Asp/Glu-rich (acidic)Add
BLAST
Compositional biasi188 – 21629Asp/Glu-rich (acidic)Add
BLAST
Compositional biasi242 – 27534Asp/Glu-rich (acidic)Add
BLAST
Compositional biasi652 – 70352Arg/Gly/Phe-richAdd
BLAST

Sequence similaritiesi

Contains 4 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IRS7. Eukaryota.
ENOG410XSFV. LUCA.
HOVERGENiHBG002295.
InParanoidiP13383.

Family and domain databases

Gene3Di3.30.70.330. 4 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 4 hits.
[Graphical view]
SMARTiSM00360. RRM. 4 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 4 hits.
PROSITEiPS50102. RRM. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P13383-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVKLAKAGKT HGESKKMAPP PKEVEEDSED EEMSEDEDDS SGEEEVVIPQ
60 70 80 90 100
KKGKKATTTP AKKVVVSQTK KAAVPTPAKK AAVTPGKKAA ATPAKKAVTP
110 120 130 140 150
AKVVPTPGKK GAAQAKALVP TPGKKGAVTP AKGAKNGKNA KKEDSDEDED
160 170 180 190 200
EEDEDDSDED EDEEDEFEPP VVKGVKPAKA APAAPASEDE DEEDDDDEDD
210 220 230 240 250
DDDDEEEEEE DDSEEEVMEI TPAKGKKTPA KVVPVKAKSV AEEEEDDEDD
260 270 280 290 300
EDEEEDEDEE DEEDDEDEDE EEEEEPVKAA PGKRKKEMTK QKEAPEAKKQ
310 320 330 340 350
KIEGSEPTTP FNLFIGNLNP NKSVAELKVA ISELFAKNDL AAVDVRTGTN
360 370 380 390 400
RKFGYVDFES AEDLEKALEL TGLKVFGNEI KLEKPKGRDS KKVRAARTLL
410 420 430 440 450
AKNLSFNITE DELKEVFEDA VEIRLVSQDG RSKGIAYIEF KSEADAEKNL
460 470 480 490 500
EEKQGAEIDG RSVSLYYTGE KGQRQERTGK NSTWSGESKT LVLSNLSYSA
510 520 530 540 550
TEETLQEVFE KATFIKVPQN PHGKSKGYAF IEFASFEDAK EALNSCNKME
560 570 580 590 600
IEGRTIRLEL QGPRGSPNAR SQPSKTLFVK GLSEDTTEET LKESFEGSVR
610 620 630 640 650
ARIVTDRETG SSKGFGFVDF NSEEDAKAAK EAMEDGEIDG NKVTLDWAKP
660 670 680 690 700
KGEGGFGGRG GGRGGFGGRG GGRGGRGGFG GRGRGGFGGR GGFRGGRGGG
710
GDFKPQGKKT KFE
Length:713
Mass (Da):77,147
Last modified:January 23, 2007 - v3
Checksum:i9652B27BFD12C8EC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M55022
, M55015, M55017, M55020 Genomic DNA. Translation: AAA41732.1.
M22090 Genomic DNA. Translation: AAA41733.1.
PIRiJH0148.
UniGeneiRn.144561.

Genome annotation databases

UCSCiRGD:3153. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M55022
, M55015, M55017, M55020 Genomic DNA. Translation: AAA41732.1.
M22090 Genomic DNA. Translation: AAA41733.1.
PIRiJH0148.
UniGeneiRn.144561.

3D structure databases

ProteinModelPortaliP13383.
SMRiP13383. Positions 302-472, 487-651.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP13383. 2 interactions.
STRINGi10116.ENSRNOP00000024712.

PTM databases

iPTMnetiP13383.
PhosphoSiteiP13383.

Proteomic databases

PaxDbiP13383.
PRIDEiP13383.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:3153. rat.

Organism-specific databases

RGDi3153. Ncl.

Phylogenomic databases

eggNOGiENOG410IRS7. Eukaryota.
ENOG410XSFV. LUCA.
HOVERGENiHBG002295.
InParanoidiP13383.

Miscellaneous databases

PROiP13383.

Family and domain databases

Gene3Di3.30.70.330. 4 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 4 hits.
[Graphical view]
SMARTiSM00360. RRM. 4 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 4 hits.
PROSITEiPS50102. RRM. 4 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleolin gene organization in rodents: highly conserved sequences within three of the 13 introns."
    Bourbon H.-M., Amalric F.
    Gene 88:187-196(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Sequence and structure of the nucleolin promoter in rodents: characterization of a strikingly conserved CpG island."
    Bourbon H.-M., Prudhomme M., Amalric F.
    Gene 68:73-84(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-45.
  3. "Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites."
    Hoffert J.D., Pisitkun T., Wang G., Shen R.-F., Knepper M.A.
    Proc. Natl. Acad. Sci. U.S.A. 103:7159-7164(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-145 AND SER-157, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  4. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-28; SER-40; SER-41; SER-145; SER-157; SER-187; SER-213; SER-305; SER-405; SER-566; SER-583 AND SER-622, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiNUCL_RAT
AccessioniPrimary (citable) accession number: P13383
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 23, 2007
Last modified: February 17, 2016
This is version 133 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.