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Protein

Glucose-6-phosphate isomerase, glycosomal

Gene

PGI

Organism
Trypanosoma brucei brucei
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-glucose 6-phosphate = D-fructose 6-phosphate.

Pathwayi: glycolysis

This protein is involved in step 2 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (Gpi), Glucose-6-phosphate isomerase, glycosomal (PGI)
  3. ATP-dependent 6-phosphofructokinase (pfk)
  4. Fructose-bisphosphate aldolase, glycosomal (ALD)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei411Proton donorBy similarity1
Active sitei442By similarity1
Active sitei571By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Gluconeogenesis, Glycolysis

Enzyme and pathway databases

BRENDAi5.3.1.9. 6519.
SABIO-RKP13377.
UniPathwayiUPA00109; UER00181.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-6-phosphate isomerase, glycosomal (EC:5.3.1.9)
Short name:
GPI
Alternative name(s):
Phosphoglucose isomerase
Short name:
PGI
Phosphohexose isomerase
Short name:
PHI
Gene namesi
Name:PGI
OrganismiTrypanosoma brucei brucei
Taxonomic identifieri5702 [NCBI]
Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeTrypanosoma

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Glycosome, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001805481 – 607Glucose-6-phosphate isomerase, glycosomalAdd BLAST607

Interactioni

Subunit structurei

Homodimer.

Structurei

Secondary structure

1607
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni49 – 52Combined sources4
Helixi54 – 66Combined sources13
Helixi71 – 77Combined sources7
Helixi81 – 84Combined sources4
Beta strandi86 – 90Combined sources5
Beta strandi98 – 107Combined sources10
Helixi110 – 122Combined sources13
Helixi125 – 133Combined sources9
Turni140 – 143Combined sources4
Helixi148 – 151Combined sources4
Beta strandi164 – 166Combined sources3
Helixi167 – 185Combined sources19
Beta strandi199 – 203Combined sources5
Helixi206 – 208Combined sources3
Helixi210 – 218Combined sources9
Helixi220 – 222Combined sources3
Beta strandi227 – 232Combined sources6
Helixi237 – 246Combined sources10
Helixi249 – 251Combined sources3
Beta strandi252 – 257Combined sources6
Beta strandi259 – 261Combined sources3
Helixi264 – 283Combined sources20
Helixi292 – 295Combined sources4
Beta strandi296 – 301Combined sources6
Helixi303 – 309Combined sources7
Helixi313 – 315Combined sources3
Beta strandi316 – 318Combined sources3
Helixi325 – 327Combined sources3
Helixi332 – 334Combined sources3
Helixi335 – 341Combined sources7
Helixi343 – 362Combined sources20
Helixi365 – 367Combined sources3
Helixi369 – 382Combined sources14
Beta strandi388 – 394Combined sources7
Helixi396 – 398Combined sources3
Helixi401 – 413Combined sources13
Beta strandi431 – 433Combined sources3
Helixi439 – 442Combined sources4
Helixi445 – 450Combined sources6
Beta strandi451 – 453Combined sources3
Beta strandi457 – 464Combined sources8
Helixi472 – 489Combined sources18
Helixi493 – 502Combined sources10
Helixi508 – 514Combined sources7
Turni515 – 518Combined sources4
Beta strandi526 – 533Combined sources8
Helixi536 – 557Combined sources22
Helixi565 – 567Combined sources3
Helixi568 – 577Combined sources10
Helixi578 – 580Combined sources3
Helixi592 – 604Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2AOLmodel-A157-562[»]
2O2CX-ray1.58A/B/C1-607[»]
2O2DX-ray1.90A/B/C1-607[»]
3CV0X-ray2.00B601-607[»]
ProteinModelPortaliP13377.
SMRiP13377.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP13377.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi605 – 607Microbody targeting signalSequence analysis3

Sequence similaritiesi

Belongs to the GPI family.Curated

Family and domain databases

Gene3Di1.10.1390.10. 1 hit.
HAMAPiMF_00473. G6P_isomerase. 1 hit.
InterProiIPR001672. G6P_Isomerase.
IPR023096. G6P_Isomerase_C.
IPR018189. Phosphoglucose_isomerase_CS.
[Graphical view]
PANTHERiPTHR11469. PTHR11469. 1 hit.
PfamiPF00342. PGI. 1 hit.
[Graphical view]
PRINTSiPR00662. G6PISOMERASE.
PROSITEiPS00765. P_GLUCOSE_ISOMERASE_1. 1 hit.
PS00174. P_GLUCOSE_ISOMERASE_2. 1 hit.
PS51463. P_GLUCOSE_ISOMERASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P13377-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSYLDDLRI DLAASPASGG SASIAVGSFN IPYEVTRRLK GVGADADTTL
60 70 80 90 100
TSCASWTQLQ KLYEQYGDEP IKKHFEADSE RGQRYSVKVS LGSKDENFLF
110 120 130 140 150
LDYSKSHIND EIKCALLRLA EERGIRQFVQ SVFRGERVNT TENRPVLHIA
160 170 180 190 200
LRNRSNRPIY VDGKDVMPAV NKVLDQMRSF SEKVRTGEWK GHTGKAIRHV
210 220 230 240 250
VNIGIGGSDL GPVMATEALK PFSQRDLSLH FVSNVDGTHI AEVLKSIDIE
260 270 280 290 300
ATLFIVASKT FTTQETITNA LSARRALLDY LRSRGIDEKG SVAKHFVALS
310 320 330 340 350
TNNQKVKEFG IDEENMFQFW DWVGGRYSMW SAIGLPIMIS IGYENFVELL
360 370 380 390 400
TGAHVIDEHF ANAPPEQNVP LLLALVGVWY INFFGAVTHA ILPYDQYLWR
410 420 430 440 450
LPAYLQQLDM ESNGKYVTRS GKTVSTLTGP IIFGEAGTNG QHAFYQLIHQ
460 470 480 490 500
GTNLIPCDFI GAIQSQNKIG DHHKIFMSNF FAQTEALMIG KSPSEVRREL
510 520 530 540 550
EAAGERSAEK INALLPHKTF IGGRPSNTLL IKSLTPRALG AIIAMYEHKV
560 570 580 590 600
LVQGAIWGID SYDQWGVELG KVLAKSILPQ LRPGMRVNNH DSSTNGLINM

FNELSHL
Length:607
Mass (Da):67,518
Last modified:January 1, 1990 - v1
Checksum:iAB237B7261CF2B74
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15540 Genomic DNA. Translation: CAA33547.1.
PIRiS06113. NUUTB.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15540 Genomic DNA. Translation: CAA33547.1.
PIRiS06113. NUUTB.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2AOLmodel-A157-562[»]
2O2CX-ray1.58A/B/C1-607[»]
2O2DX-ray1.90A/B/C1-607[»]
3CV0X-ray2.00B601-607[»]
ProteinModelPortaliP13377.
SMRiP13377.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00109; UER00181.
BRENDAi5.3.1.9. 6519.
SABIO-RKP13377.

Miscellaneous databases

EvolutionaryTraceiP13377.

Family and domain databases

Gene3Di1.10.1390.10. 1 hit.
HAMAPiMF_00473. G6P_isomerase. 1 hit.
InterProiIPR001672. G6P_Isomerase.
IPR023096. G6P_Isomerase_C.
IPR018189. Phosphoglucose_isomerase_CS.
[Graphical view]
PANTHERiPTHR11469. PTHR11469. 1 hit.
PfamiPF00342. PGI. 1 hit.
[Graphical view]
PRINTSiPR00662. G6PISOMERASE.
PROSITEiPS00765. P_GLUCOSE_ISOMERASE_1. 1 hit.
PS00174. P_GLUCOSE_ISOMERASE_2. 1 hit.
PS51463. P_GLUCOSE_ISOMERASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiG6PI_TRYBB
AccessioniPrimary (citable) accession number: P13377
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: November 2, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.