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Protein

Glucose-6-phosphate isomerase, glycosomal

Gene

PGI

Organism
Trypanosoma brucei brucei
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-glucose 6-phosphate = D-fructose 6-phosphate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei411 – 4111Proton donorBy similarity
Active sitei442 – 4421By similarity
Active sitei571 – 5711By similarity

GO - Molecular functioni

  1. glucose-6-phosphate isomerase activity Source: UniProtKB-EC

GO - Biological processi

  1. gluconeogenesis Source: UniProtKB-KW
  2. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Gluconeogenesis, Glycolysis

Enzyme and pathway databases

BRENDAi5.3.1.9. 6519.
SABIO-RKP13377.
UniPathwayiUPA00109; UER00181.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-6-phosphate isomerase, glycosomal (EC:5.3.1.9)
Short name:
GPI
Alternative name(s):
Phosphoglucose isomerase
Short name:
PGI
Phosphohexose isomerase
Short name:
PHI
Gene namesi
Name:PGI
OrganismiTrypanosoma brucei brucei
Taxonomic identifieri5702 [NCBI]
Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeTrypanosoma

Subcellular locationi

GO - Cellular componenti

  1. glycosome Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Glycosome, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 607607Glucose-6-phosphate isomerase, glycosomalPRO_0000180548Add
BLAST

Interactioni

Subunit structurei

Homodimer.

Structurei

Secondary structure

1
607
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni49 – 524Combined sources
Helixi54 – 6613Combined sources
Helixi71 – 777Combined sources
Helixi81 – 844Combined sources
Beta strandi86 – 905Combined sources
Beta strandi98 – 10710Combined sources
Helixi110 – 12213Combined sources
Helixi125 – 1339Combined sources
Turni140 – 1434Combined sources
Helixi148 – 1514Combined sources
Beta strandi164 – 1663Combined sources
Helixi167 – 18519Combined sources
Beta strandi199 – 2035Combined sources
Helixi206 – 2083Combined sources
Helixi210 – 2189Combined sources
Helixi220 – 2223Combined sources
Beta strandi227 – 2326Combined sources
Helixi237 – 24610Combined sources
Helixi249 – 2513Combined sources
Beta strandi252 – 2576Combined sources
Beta strandi259 – 2613Combined sources
Helixi264 – 28320Combined sources
Helixi292 – 2954Combined sources
Beta strandi296 – 3016Combined sources
Helixi303 – 3097Combined sources
Helixi313 – 3153Combined sources
Beta strandi316 – 3183Combined sources
Helixi325 – 3273Combined sources
Helixi332 – 3343Combined sources
Helixi335 – 3417Combined sources
Helixi343 – 36220Combined sources
Helixi365 – 3673Combined sources
Helixi369 – 38214Combined sources
Beta strandi388 – 3947Combined sources
Helixi396 – 3983Combined sources
Helixi401 – 41313Combined sources
Beta strandi431 – 4333Combined sources
Helixi439 – 4424Combined sources
Helixi445 – 4506Combined sources
Beta strandi451 – 4533Combined sources
Beta strandi457 – 4648Combined sources
Helixi472 – 48918Combined sources
Helixi493 – 50210Combined sources
Helixi508 – 5147Combined sources
Turni515 – 5184Combined sources
Beta strandi526 – 5338Combined sources
Helixi536 – 55722Combined sources
Helixi565 – 5673Combined sources
Helixi568 – 57710Combined sources
Helixi578 – 5803Combined sources
Helixi592 – 60413Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2AOLmodel-A157-562[»]
2O2CX-ray1.58A/B/C1-607[»]
2O2DX-ray1.90A/B/C1-607[»]
3CV0X-ray2.00B601-607[»]
ProteinModelPortaliP13377.
SMRiP13377. Positions 38-606.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP13377.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi605 – 6073Microbody targeting signalSequence Analysis

Sequence similaritiesi

Belongs to the GPI family.Curated

Family and domain databases

Gene3Di1.10.1390.10. 1 hit.
HAMAPiMF_00473. G6P_isomerase.
InterProiIPR001672. G6P_Isomerase.
IPR023096. G6P_Isomerase_C.
IPR018189. Phosphoglucose_isomerase_CS.
[Graphical view]
PANTHERiPTHR11469. PTHR11469. 1 hit.
PfamiPF00342. PGI. 1 hit.
[Graphical view]
PRINTSiPR00662. G6PISOMERASE.
PROSITEiPS00765. P_GLUCOSE_ISOMERASE_1. 1 hit.
PS00174. P_GLUCOSE_ISOMERASE_2. 1 hit.
PS51463. P_GLUCOSE_ISOMERASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P13377-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSYLDDLRI DLAASPASGG SASIAVGSFN IPYEVTRRLK GVGADADTTL
60 70 80 90 100
TSCASWTQLQ KLYEQYGDEP IKKHFEADSE RGQRYSVKVS LGSKDENFLF
110 120 130 140 150
LDYSKSHIND EIKCALLRLA EERGIRQFVQ SVFRGERVNT TENRPVLHIA
160 170 180 190 200
LRNRSNRPIY VDGKDVMPAV NKVLDQMRSF SEKVRTGEWK GHTGKAIRHV
210 220 230 240 250
VNIGIGGSDL GPVMATEALK PFSQRDLSLH FVSNVDGTHI AEVLKSIDIE
260 270 280 290 300
ATLFIVASKT FTTQETITNA LSARRALLDY LRSRGIDEKG SVAKHFVALS
310 320 330 340 350
TNNQKVKEFG IDEENMFQFW DWVGGRYSMW SAIGLPIMIS IGYENFVELL
360 370 380 390 400
TGAHVIDEHF ANAPPEQNVP LLLALVGVWY INFFGAVTHA ILPYDQYLWR
410 420 430 440 450
LPAYLQQLDM ESNGKYVTRS GKTVSTLTGP IIFGEAGTNG QHAFYQLIHQ
460 470 480 490 500
GTNLIPCDFI GAIQSQNKIG DHHKIFMSNF FAQTEALMIG KSPSEVRREL
510 520 530 540 550
EAAGERSAEK INALLPHKTF IGGRPSNTLL IKSLTPRALG AIIAMYEHKV
560 570 580 590 600
LVQGAIWGID SYDQWGVELG KVLAKSILPQ LRPGMRVNNH DSSTNGLINM

FNELSHL
Length:607
Mass (Da):67,518
Last modified:January 1, 1990 - v1
Checksum:iAB237B7261CF2B74
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15540 Genomic DNA. Translation: CAA33547.1.
PIRiS06113. NUUTB.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15540 Genomic DNA. Translation: CAA33547.1.
PIRiS06113. NUUTB.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2AOLmodel-A157-562[»]
2O2CX-ray1.58A/B/C1-607[»]
2O2DX-ray1.90A/B/C1-607[»]
3CV0X-ray2.00B601-607[»]
ProteinModelPortaliP13377.
SMRiP13377. Positions 38-606.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00109; UER00181.
BRENDAi5.3.1.9. 6519.
SABIO-RKP13377.

Miscellaneous databases

EvolutionaryTraceiP13377.

Family and domain databases

Gene3Di1.10.1390.10. 1 hit.
HAMAPiMF_00473. G6P_isomerase.
InterProiIPR001672. G6P_Isomerase.
IPR023096. G6P_Isomerase_C.
IPR018189. Phosphoglucose_isomerase_CS.
[Graphical view]
PANTHERiPTHR11469. PTHR11469. 1 hit.
PfamiPF00342. PGI. 1 hit.
[Graphical view]
PRINTSiPR00662. G6PISOMERASE.
PROSITEiPS00765. P_GLUCOSE_ISOMERASE_1. 1 hit.
PS00174. P_GLUCOSE_ISOMERASE_2. 1 hit.
PS51463. P_GLUCOSE_ISOMERASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Glucosephosphate isomerase from Trypanosoma brucei. Cloning and characterization of the gene and analysis of the enzyme."
    Marchand M., Kooystra U., Wierenga R.K., Lambeir A.-M., van Beeumen J., Opperdoes F.R., Michels P.A.M.
    Eur. J. Biochem. 184:455-464(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
    Strain: 427.

Entry informationi

Entry nameiG6PI_TRYBB
AccessioniPrimary (citable) accession number: P13377
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: April 1, 2015
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.