Reviewed, UniProtKB/Swiss-Prot P13368 (7LESS_DROME)

Names and origin

Protein names

Recommended name:
    Protein sevenless
    EC=2.7.10.1

Gene names

Name:	sev
Synonyms:	HD-265
ORF Names:	CG18085

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	2554 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Receptor for an extracellular signal required to instruct a cell to differentiate into an R7 photoreceptor. The ligand for sev is the boss (bride of sevenless) protein on the surface of the neighboring R8 cell. Ref.1
Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Subunit structure	May form a complex with drk and Sos. Binds the phosphotyrosine interaction domain (PID) of Dab.
Subcellular location	Cell membrane; Single-pass membrane protein Potential.
Domain	It is unclear whether the potential membrane spanning region near the N-terminus is present as a transmembrane domain in the native protein or serves as a cleaved signal sequence.
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily. Contains 7 fibronectin type-III domains. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Sensory transduction Vision
Cellular component	Cell membrane Membrane
Domain	Repeat Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Receptor Transferase Tyrosine-protein kinase
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	R7 cell development Non-traceable author statement. Source: FlyBase R8 cell fate specification Inferred from mutant phenotype. Source: FlyBase determination of anterior/posterior axis, embryo Traceable author statement. Source: FlyBase positive regulation of photoreceptor cell differentiation Inferred from mutant phenotype. Source: FlyBase protein amino acid phosphorylation Non-traceable author statement. Source: FlyBase response to stimulus Inferred from electronic annotation. Source: UniProtKB-KW torso signaling pathway Non-traceable author statement. Source: FlyBase visual perception Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from direct assay. Source: FlyBase
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Ref.7 Inferred from physical interaction. Source: UniProtKB transmembrane receptor protein tyrosine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2554	2554	Protein sevenless		PRO_0000058928
Regions
	Topological domain	1 – 2123	2123	Extracellular Potential
	Transmembrane	2124 – 2147	24	Potential
	Topological domain	2148 – 2554	407	Cytoplasmic Potential
	Domain	311 – 431	121	Fibronectin type-III 1
	Domain	436 – 528	93	Fibronectin type-III 2
	Domain	822 – 921	100	Fibronectin type-III 3
	Domain	1298 – 1392	95	Fibronectin type-III 4
	Domain	1680 – 1794	115	Fibronectin type-III 5
	Domain	1797 – 1897	101	Fibronectin type-III 6
	Domain	1898 – 1988	91	Fibronectin type-III 7
	Domain	2209 – 2485	277	Protein kinase
	Nucleotide binding	2215 – 2223	9	ATP By similarity
	Compositional bias	2038 – 2046	9	Poly-Arg
Sites
	Active site	2343	1	Proton acceptor By similarity
	Binding site	2242	1	ATP By similarity
Amino acid modifications
	Modified residue	2380	1	Phosphotyrosine; by autocatalysis By similarity
	Glycosylation	30	1	N-linked (GlcNAc...) Potential
	Glycosylation	129	1	N-linked (GlcNAc...) Potential
	Glycosylation	481	1	N-linked (GlcNAc...) Potential
	Glycosylation	505	1	N-linked (GlcNAc...) Potential
	Glycosylation	617	1	N-linked (GlcNAc...) Potential
	Glycosylation	647	1	N-linked (GlcNAc...) Potential
	Glycosylation	966	1	N-linked (GlcNAc...) Potential
	Glycosylation	1228	1	N-linked (GlcNAc...) Potential
	Glycosylation	1313	1	N-linked (GlcNAc...) Potential
	Glycosylation	1353	1	N-linked (GlcNAc...) Potential
	Glycosylation	1550	1	N-linked (GlcNAc...) Potential
	Glycosylation	1557	1	N-linked (GlcNAc...) Potential
	Glycosylation	1639	1	N-linked (GlcNAc...) Potential
	Glycosylation	1725	1	N-linked (GlcNAc...) Potential
	Glycosylation	1756	1	N-linked (GlcNAc...) Potential
	Glycosylation	1804	1	N-linked (GlcNAc...) Potential
	Glycosylation	1889	1	N-linked (GlcNAc...) Potential
	Glycosylation	1947	1	N-linked (GlcNAc...) Potential
	Glycosylation	2073	1	N-linked (GlcNAc...) Potential
Experimental info
	Mutagenesis	2242	1	K → M: Inactivates the protein. Ref.1
	Sequence conflict	392	1	V → M in AAA28882. Ref.1
	Sequence conflict	663	1	A → T in AAF47992. Ref.3
	Sequence conflict	1703	1	N → H in AAF47992. Ref.3
	Sequence conflict	1730 – 1731	2	RG → KE in AAF47992. Ref.3
	Sequence conflict	1741	1	V → M in AAF47992. Ref.3
	Sequence conflict	1823	1	E → Q in CAA31960. Ref.2
	Sequence conflict	1823	1	E → Q in CAB55310. Ref.2
	Sequence conflict	2271	1	C → R in AAA28882. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P13368-1 [UniParc]. Last modified June 20, 2001. Version 2. Checksum: 09E238A0F27684F8	FASTA	2,554	287,025
	10 20 30 40 50 60 MTMFWQQNVD HQSDEQDKQA KGAAPTKRLN ISFNVKIAVN VNTKMTTTHI NQQAPGTSSS 70 80 90 100 110 120 SSNSQNASPS KIVVRQQSSS FDLRQQLARL GRQLASGQDG HGGISTILII NLLLLILLSI 130 140 150 160 170 180 CCDVCRSHNY TVHQSPEPVS KDQMRLLRPK LDSDVVEKVA IWHKHAAAAP PSIVEGIAIS 190 200 210 220 230 240 SRPQSTMAHH PDDRDRDRDP SEEQHGVDER MVLERVTRDC VQRCIVEEDL FLDEFGIQCE 250 260 270 280 290 300 KADNGEKCYK TRCTKGCAQW YRALKELESC QEACLSLQFY PYDMPCIGAC EMAQRDYWHL 310 320 330 340 350 360 QRLAISHLVE RTQPQLERAP RADGQSTPLT IRWAMHFPEH YLASRPFNIQ YQFVDHHGEE 370 380 390 400 410 420 LDLEQEDQDA SGETGSSAWF NLADYDCDEY YVCEILEALI PYTQYRFRFE LPFGENRDEV 430 440 450 460 470 480 LYSPATPAYQ TPPEGAPISA PVIEHLMGLD DSHLAVHWHP GRFTNGPIEG YRLRLSSSEG 490 500 510 520 530 540 NATSEQLVPA GRGSYIFSQL QAGTNYTLAL SMINKQGEGP VAKGFVQTHS ARNEKPAKDL 550 560 570 580 590 600 TESVLLVGRR AVMWQSLEPA GENSMIYQSQ EELADIAWSK REQQLWLLNV HGELRSLKFE 610 620 630 640 650 660 SGQMVSPAQQ LKLDLGNISS GRWVPRRLSF DWLHHRLYFA MESPERNQSS FQIISTDLLG 670 680 690 700 710 720 ESAQKVGESF DLPVEQLEVD ALNGWIFWRN EESLWRQDLH GRMIHRLLRI RQPGWFLVQP 730 740 750 760 770 780 QHFIIHLMLP QEGKFLEISY DGGFKHPLPL PPPSNGAGNG PASSHWQSFA LLGRSLLLPD 790 800 810 820 830 840 SGQLILVEQQ GQAASPSASW PLKNLPDCWA VILLVPESQP LTSAGGKPHS LKALLGAQAA 850 860 870 880 890 900 KISWKEPERN PYQSADAARS WSYELEVLDV ASQSAFSIRN IRGPIFGLQR LQPDNLYQLR 910 920 930 940 950 960 VRAINVDGEP GEWTEPLAAR TWPLGPHRLR WASRQGSVIH TNELGEGLEV QQEQLERLPG 970 980 990 1000 1010 1020 PMTMVNESVG YYVTGDGLLH CINLVHSQWG CPISEPLQHV GSVTYDWRGG RVYWTDLARN 1030 1040 1050 1060 1070 1080 CVVRMDPWSG SRELLPVFEA NFLALDPRQG HLYYATSSQL SRHGSTPDEA VTYYRVNGLE 1090 1100 1110 1120 1130 1140 GSIASFVLDT QQDQLFWLVK GSGALRLYRA PLTAGGDSLQ MIQQIKGVFQ AVPDSLQLLR 1150 1160 1170 1180 1190 1200 PLGALLWLER SGRRARLVRL AAPLDVMELP TPDQASPASA LQLLDPQPLP PRDEGVIPMT 1210 1220 1230 1240 1250 1260 VLPDSVRLDD GHWDDFHVRW QPSTSGGNHS VSYRLLLEFG QRLQTLDLST PFARLTQLPQ 1270 1280 1290 1300 1310 1320 AQLQLKISIT PRTAWRSGDT TRVQLTTPPV APSQPRRLRV FVERLATALQ EANVSAVLRW 1330 1340 1350 1360 1370 1380 DAPEQGQEAP MQALEYHISC WVGSELHEEL RLNQSALEAR VEHLQPDQTY HFQVEARVAA 1390 1400 1410 1420 1430 1440 TGAAAGAASH ALHVAPEVQA VPRVLYANAE FIGELDLDTR NRRRLVHTAS PVEHLVGIEG 1450 1460 1470 1480 1490 1500 EQRLLWVNEH VELLTHVPGS APAKLARMRA EVLALAVDWI QRIVYWAELD ATAPQAAIIY 1510 1520 1530 1540 1550 1560 RLDLCNFEGK ILQGERVWST PRGRLLKDLV ALPQAQSLIW LEYEQGSPRN GSLRGRNLTD 1570 1580 1590 1600 1610 1620 GSELEWATVQ PLIRLHAGSL EPGSETLNLV DNQGKLCVYD VARQLCTASA LRAQLNLLGE 1630 1640 1650 1660 1670 1680 DSIAGQLAQD SGYLYAVKNW SIRAYGRRRQ QLEYTVELEP EEVRLLQAHN YQAYPPKNCL 1690 1700 1710 1720 1730 1740 LLPSSGGSLL KATDCEEQRC LLNLPMITAS EDCPLPIPGV RYQLNLTLAR GPGSEEHDHG 1750 1760 1770 1780 1790 1800 VEPLGQWLLG AGESLNLTDL LPFTRYRVSG ILSSFYQKKL ALPTLVLAPL ELLTASATPS 1810 1820 1830 1840 1850 1860 PPRNFSVRVL SPRELEVSWL PPEQLRSESV YYTLHWQQEL DGENVQDRRE WEAHERRLET 1870 1880 1890 1900 1910 1920 AGTHRLTGIK PGSGYSLWVQ AHATPTKSNS SERLHVRSFA ELPELQLLEL GPYSLSLTWA 1930 1940 1950 1960 1970 1980 GTPDPLGSLQ LECRSSAEQL RRNVAGNHTK MVVEPLQPRT RYQCRLLLGY AATPGAPLYH 1990 2000 2010 2020 2030 2040 GTAEVYETLG DAPSQPGKPQ LEHIAEEVFR VTWTAARGNG APIALYNLEA LQARSDIRRR 2050 2060 2070 2080 2090 2100 RRRRRRNSGG SLEQLPWAEE PVVVEDQWLD FCNTTELSCI VKSLHSSRLL LFRVRARSLE 2110 2120 2130 2140 2150 2160 HGWGPYSEES ERVAEPFVSP EKRGSLVLAI IAPAAIVSSC VLALVLVRKV QKRRLRAKKL 2170 2180 2190 2200 2210 2220 LQQSRPSIWS NLSTLQTQQQ LMAVRNRAFS TTLSDADIAL LPQINWSQLK LLRFLGSGAF 2230 2240 2250 2260 2270 2280 GEVYEGQLKT EDSEEPQRVA IKSLRKGASE FAELLQEAQL MSNFKHENIV CLVGICFDTE 2290 2300 2310 2320 2330 2340 SISLIMEHME AGDLLSYLRA ARATSTQEPQ PTAGLSLSEL LAMCIDVANG CSYLEDMHFV 2350 2360 2370 2380 2390 2400 HRDLACRNCL VTESTGSTDR RRTVKIGDFG LARDIYKSDY YRKEGEGLLP VRWMSPESLV 2410 2420 2430 2440 2450 2460 DGLFTTQSDV WAFGVLCWEI LTLGQQPYAA RNNFEVLAHV KEGGRLQQPP MCTEKLYSLL 2470 2480 2490 2500 2510 2520 LLCWRTDPWE RPSFRRCYNT LHAISTDLRR TQMASATADT VVSCSRPEFK VRFDGQPLEE 2530 2540 2550 HREHNERPED ENLTLREVPL KDKQLYANEG VSRL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Control of photoreceptor cell fate by the sevenless protein requires a functional tyrosine kinase domain." Basler K., Hafen E. Cell 54:299-311(1988) [PubMed: 2840202] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, MUTAGENESIS OF LYS-2242. Strain: Canton-S.
[2]	"Nucleotide sequence and structure of the sevenless gene of Drosophila melanogaster." Bowtell D.L.L., Simon M.A., Rubin G.M. Genes Dev. 2:620-634(1988) [PubMed: 3138161] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Oregon-R.
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[5]	"Sampling the genomic pool of protein tyrosine kinase genes using the polymerase chain reaction with genomic DNA." Oates A.C., Wollberg P., Achen M.G., Wilks A.F. Biochem. Biophys. Res. Commun. 249:660-667(1998) [PubMed: 9731193] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 2349-2408.
[6]	"Sevenless: seven found?" Norton P.A., Hynes R.O., Ress D.J.G. Cell 61:15-16(1990) [PubMed: 2317871] [Abstract] Cited for: IDENTIFICATION OF FN-III REPEATS.
[7]	"Disabled is a putative adaptor protein that functions during signaling by the sevenless receptor tyrosine kinase." Le N., Simon M.A. Mol. Cell. Biol. 18:4844-4854(1998) [PubMed: 9671493] [Abstract] Cited for: INTERACTION WITH DAB.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	J03158 Genomic DNA. Translation: AAA28882.1. X13666 mRNA. Translation: CAA31960.1. Different initiation. X13666 mRNA. Translation: CAB55310.1. AE014298 Genomic DNA. Translation: AAF47992.2. AJ002917 Genomic DNA. Translation: CAA05752.1.
PIR	TVFF7L. A28912.
RefSeq	NP_511114.2.
UniGene	Dm.2618
3D structure databases
HSSP	HSSP built from PDB template 1JQH based on UniProtKB P08069.
ModBase	Search...
Protein-protein interaction databases
IntAct	P13368. 5 interactions.
Genome annotation databases
Ensembl	FBgn0003366. Drosophila melanogaster. [Contig view]
GeneID	32039.
KEGG	dme:Dmel_CG18085.
Organism-specific databases
FlyBase	FBgn0003366. sev.
Phylogenomic databases
HOGENOM	P13368.
Enzyme and pathway databases
BRENDA	2.7.10.1. 48.
Gene expression databases
ArrayExpress	P13368.
GermOnline	CG18085. Drosophila melanogaster.
Family and domain databases
InterPro	IPR011042. 6-blade_b-propeller_TolB-like. IPR008957. Fibronectin_typ-III-like_fold. IPR003961. FN_III. IPR000033. LDLR. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR002011. Recept_tyr_kinase-II_CS. IPR001245. Tyr_pkinase. IPR008266. Tyr_pkinase_AS. [Graphical view]
Gene3D	G3DSA:2.120.10.30. 6-blade_b-propeller_TolB-like. 1 hit. G3DSA:2.60.40.30. FN_III-like. 3 hits.
Pfam	PF00041. fn3. 6 hits. PF07714. Pkinase_Tyr. 1 hit. [Graphical view]
PRINTS	PR00109. TYRKINASE.
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00060. FN3. 7 hits. SM00135. LY. 2 hits. SM00219. TyrKc. 1 hit. [Graphical view]
PROSITE	PS50853. FN3. 7 hits. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. PS00239. RECEPTOR_TYR_KIN_II. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	776500.

Entry information

Entry name

7LESS_DROME

Accession

Primary (citable) accession number: P13368
Secondary accession number(s): Q9TYI0, Q9U5V7, Q9VZ36

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	June 20, 2001
Last modified:	June 16, 2009
This is version 102 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project