P13365 (CG13_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
G1/S-specific cyclin CLN3

Gene names

Name:	CLN3
Synonyms:	DAF1, WHI1
Ordered Locus Names:	YAL040C
ORF Names:	FUN10

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	580 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential for the control of the cell cycle at the G1/S (start) transition. CLN3 may be an upstream activator of the G1 cyclins which directly catalyze start.
Induction	Not significantly cell cycle regulated.
Sequence similarities	Belongs to the cyclin family.

Ontologies

Keywords
Biological process	Cell cycle Cell division
Molecular function	Cyclin
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell division Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell size Inferred from mutant phenotype Ref.2. Source: SGD regulation of cyclin-dependent protein serine/threonine kinase activity Inferred from mutant phenotype PubMed 1316273PubMed 8387915. Source: SGD regulation of transcription involved in G1/S transition of mitotic cell cycle Inferred from mutant phenotype PubMed 19823668. Source: SGD vacuole fusion, non-autophagic Inferred from direct assay PubMed 14573462. Source: SGD vacuole organization Inferred from mutant phenotype PubMed 14573462. Source: SGD
Cellular_component	nucleus Inferred from direct assay PubMed 10611233PubMed 11509671PubMed 11792824. Source: SGD
Molecular_function	cyclin-dependent protein serine/threonine kinase regulator activity Inferred from direct assay PubMed 1316273. Source: SGD protein binding Inferred from physical interaction PubMed 10688190PubMed 14690591PubMed 20489023PubMed 23217712. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CDC28	P00546	5	EBI-4490,EBI-4253

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 580	580	G1/S-specific cyclin CLN3		PRO_0000080413
Experimental info
	Sequence conflict	23	1	S → P in AAU09673. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P13365 [UniParc]. Last modified April 1, 1990. Version 2. Checksum: AF6950A7E68DB295	FASTA	580	64,991
	10 20 30 40 50 60 MAILKDTIIR YANARYATAS GTSTATAASV SAASCPNLPL LLQKRRAIAS AKSKNPNLVK 70 80 90 100 110 120 RELQAHHSAI SEYNNDQLDH YFRLSHTERP LYNLTNFNSQ PQVNPKMRFL IFDFIMYCHT 130 140 150 160 170 180 RLNLSTSTLF LTFTILDKYS SRFIIKSYNY QLLSLTALWI SSKFWDSKNR MATLKVLQNL 190 200 210 220 230 240 CCNQYSIKQF TTMEMHLFKS LDWSICQSAT FDSYIDIFLF QSTSPLSPGV VLSAPLEAFI 250 260 270 280 290 300 QQKLALLNNA AGTAINKSSS SQGPSLNINE IKLGAIMLCE LASFNLELSF KYDRSLIALG 310 320 330 340 350 360 AINLIKLSLN YYNSNLWENI NLALEENCQD LDIKLSEISN TLLDIAMDQN SFPSSFKSKY 370 380 390 400 410 420 LNSNKTSLAK SLLDALQNYC IQLKLEEFYR SQELETMYNT IFAQSFDSDS LTCVYSNATT 430 440 450 460 470 480 PKSATVSSAA TDYFSDHTHL RRLTKDSISP PFAFTPTSSS SSPSPFNSPY KTSSSMTTPD 490 500 510 520 530 540 SASHHSHSGS FSSTQNSFKR SLSIPQNSSI FWPSPLTPTT PSLMSNRKLL QNLSVRSKRL 550 560 570 580 FPVRPMATAH PCSAPTQLKK RSTSSVDCDF NDSSNLKKTR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The WHI1+ gene of Saccharomyces cerevisiae tethers cell division to cell size and is a cyclin homolog." Nash R., Tokiwa G., Anand S., Erickson C., Futcher A.B. EMBO J. 7:4335-4346(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S673A.
[2]	"DAF1, a mutant gene affecting size control, pheromone arrest, and cell cycle kinetics of Saccharomyces cerevisiae." Cross F.R. Mol. Cell. Biol. 8:4675-4684(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The nucleotide sequence of chromosome I from Saccharomyces cerevisiae." Bussey H., Kaback D.B., Zhong W.-W., Vo D.H., Clark M.W., Fortin N., Hall J., Ouellette B.F.F., Keng T., Barton A.B., Su Y., Davies C.J., Storms R.K. Proc. Natl. Acad. Sci. U.S.A. 92:3809-3813(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"The reference genome sequence of Saccharomyces cerevisiae: Then and now." Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M. G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X13964 Genomic DNA. Translation: CAA32143.1. M23359 Genomic DNA. Translation: AAA34552.1. M23359 Genomic DNA. Translation: AAA34551.1. Different termination. U12980 Genomic DNA. Translation: AAC04991.1. AY723756 Genomic DNA. Translation: AAU09673.1. BK006935 Genomic DNA. Translation: DAA06946.1.
PIR	S14054.
RefSeq	NP_009360.1. NM_001178185.1.
3D structure databases
ProteinModelPortal	P13365.
ModBase	Search...
MobiDB	Search...
Protein-protein interaction databases
BioGrid	31725. 165 interactions.
DIP	DIP-1267N.
IntAct	P13365. 13 interactions.
MINT	MINT-396526.
STRING	4932.YAL040C.
Proteomic databases
PeptideAtlas	P13365.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YAL040C; YAL040C; YAL040C.
GeneID	851191.
KEGG	sce:YAL040C.
Organism-specific databases
CYGD	YAL040c.
SGD	S000000038. CLN3.
Phylogenomic databases
eggNOG	COG5024.
KO	K06646.
OrthoDB	EOG7712K0.
Enzyme and pathway databases
BioCyc	YEAST:G3O-28848-MONOMER.
Gene expression databases
Genevestigator	P13365.
Family and domain databases
Gene3D	1.10.472.10. 1 hit.
InterPro	IPR013763. Cyclin-like. IPR006671. Cyclin_N. [Graphical view]
Pfam	PF00134. Cyclin_N. 1 hit. [Graphical view]
SMART	SM00385. CYCLIN. 1 hit. [Graphical view]
SUPFAM	SSF47954. SSF47954. 1 hit.
PROSITE	PS00292. CYCLINS. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	968031.

Entry information

Entry name

CG13_YEAST

Accession

Primary (citable) accession number: P13365
Secondary accession number(s): D6VPH6, E9P940

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	April 1, 1990
Last modified:	June 11, 2014
This is version 116 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program