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P13340 (END7_BPT4) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Recombination endonuclease VII

Short name=Endo VII
EC=3.1.-.-
Alternative name(s):
Protein Gp49
Gene names
Name:49
OrganismEnterobacteria phage T4 (Bacteriophage T4) [Reference proteome]
Taxonomic identifier10665 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeTevenvirinaeT4likevirus
Virus hostEscherichia coli [TaxID: 562]

Protein attributes

Sequence length157 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cleaves DNA cruciform and Y-structures as well as heteroduplex loops. Resolves Holliday junctions, recognizes a broad spectrum of DNA substrates ranging from branched DNAs to single base mismatches.

Cofactor

Binds 1 calcium ion per subunit.

Binds 1 zinc ion per subunit.

Subunit structure

Homodimer.

Ontologies

Keywords
   Coding sequence diversityAlternative initiation
   LigandCalcium
Metal-binding
Zinc
   Molecular functionEndonuclease
Hydrolase
Nuclease
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Molecular_functionendonuclease activity

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]
Isoform Long (identifier: P13340-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Short (identifier: P13340-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-52: Missing.
     53-53: V → M

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 157157Recombination endonuclease VII
PRO_0000003326

Sites

Metal binding231Zinc
Metal binding261Zinc
Metal binding401Calcium
Metal binding581Zinc
Metal binding611Zinc
Metal binding621Calcium

Natural variations

Alternative sequence1 – 5252Missing in isoform Short.
VSP_018678
Alternative sequence531V → M in isoform Short.
VSP_018986

Secondary structure

........................... 157
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform Long [UniParc].

Last modified January 1, 1990. Version 1.
Checksum: 86233D2A7FC0F9A8

FASTA15718,144
        10         20         30         40         50         60 
MLLTGKLYKE EKQKFYDAQN GKCLICQREL NPDVQANHLD HDHELNGPKA GKVRGLLCNL 

        70         80         90        100        110        120 
CNAAEGQMKH KFNRSGLKGQ GVDYLEWLEN LLTYLKSDYT QNNIHPNFVG DKSKEFSRLG 

       130        140        150 
KEEMMAEMLQ RGFEYNESDT KTQLIASFKK QLRKSLK 

« Hide

Isoform Short [UniParc].

Checksum: B2EDCD7FA9B65546
Show »

FASTA10512,213

References

« Hide 'large scale' references
[1]"Nucleotide sequence and primary structures of gene products coded for by the T4 genome between map positions 48.266 kb and 39.166 kb."
Tomaschewski J., Rueger W.
Nucleic Acids Res. 15:3632-3633(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: C.
[2]"Regulation of two nested proteins from gene 49 (recombination endonuclease VII) and of a lambda RexA-like protein of bacteriophage T4."
Barth K.A., Powell D., Trupin M., Mosig G.
Genetics 120:329-343(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE INITIATION.
[3]"Bacteriophage T4 genome."
Miller E.S., Kutter E., Mosig G., Arisaka F., Kunisawa T., Ruger W.
Microbiol. Mol. Biol. Rev. 67:86-156(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"Large-scale preparation of T4 endonuclease VII from over-expressing bacteria."
Kosak H.G., Kemper B.W.
Eur. J. Biochem. 194:779-784(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[5]"X-ray structure of T4 endonuclease VII: a DNA junction resolvase with a novel fold and unusual domain-swapped dimer architecture."
Raaijmakers H., Vix O., Toro I., Golz S., Kemper B., Suck D.
EMBO J. 18:1447-1458(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X12629 Genomic DNA. Translation: CAA31148.1.
X12629 Genomic DNA. Translation: CAA31149.1.
Y00122 Genomic DNA. Translation: CAA68307.1.
AF158101 Genomic DNA. Translation: AAD42477.1.
AF158101 Genomic DNA. Translation: AAD42687.2.
PIRZNBPT9. S01906.
RefSeqNP_049692.1. NC_000866.4. [P13340-1]
NP_049693.2. NC_000866.4. [P13340-2]

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1E7DX-ray2.80A/B1-157[»]
1E7LX-ray1.32A/B1-157[»]
1EN7X-ray2.40A/B1-157[»]
2QNCX-ray3.10A/B1-157[»]
2QNFX-ray3.00A/B1-157[»]
ProteinModelPortalP13340.
SMRP13340. Positions 1-157.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-60245N.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1258702.
1258772.

Enzyme and pathway databases

BRENDA3.1.22.4. 732.

Family and domain databases

InterProIPR004211. Endonuclease_7.
IPR015208. T4_recomb_endonuclease_dimer.
[Graphical view]
PfamPF09124. Endonuc-dimeris. 1 hit.
PF02945. Endonuclease_7. 1 hit.
[Graphical view]
SUPFAMSSF68918. SSF68918. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP13340.

Entry information

Entry nameEND7_BPT4
AccessionPrimary (citable) accession number: P13340
Secondary accession number(s): Q38426, Q9T0V4
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: May 14, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references