Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P13340

- END7_BPT4

UniProt

P13340 - END7_BPT4

Protein

Recombination endonuclease VII

Gene

49

Organism
Enterobacteria phage T4 (Bacteriophage T4)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Cleaves DNA cruciform and Y-structures as well as heteroduplex loops. Resolves Holliday junctions, recognizes a broad spectrum of DNA substrates ranging from branched DNAs to single base mismatches.

    Cofactori

    Binds 1 calcium ion per subunit.
    Binds 1 zinc ion per subunit.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi23 – 231Zinc
    Metal bindingi26 – 261Zinc
    Metal bindingi40 – 401Calcium
    Metal bindingi58 – 581Zinc
    Metal bindingi61 – 611Zinc
    Metal bindingi62 – 621Calcium

    GO - Molecular functioni

    1. endonuclease activity Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Ligandi

    Calcium, Metal-binding, Zinc

    Enzyme and pathway databases

    BRENDAi3.1.22.4. 732.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Recombination endonuclease VII (EC:3.1.-.-)
    Short name:
    Endo VII
    Alternative name(s):
    Gene product 49
    Short name:
    gp49
    Gene namesi
    Name:49
    OrganismiEnterobacteria phage T4 (Bacteriophage T4)
    Taxonomic identifieri10665 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeTevenvirinaeT4likevirus
    Virus hostiEscherichia coli [TaxID: 562]
    ProteomesiUP000009087: Genome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 157157Recombination endonuclease VIIPRO_0000003326Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.

    Protein-protein interaction databases

    DIPiDIP-60245N.

    Structurei

    Secondary structure

    1
    157
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi6 – 1813
    Turni19 – 213
    Turni24 – 263
    Helixi34 – 363
    Beta strandi37 – 415
    Beta strandi45 – 473
    Turni48 – 514
    Beta strandi52 – 576
    Helixi59 – 7416
    Beta strandi75 – 773
    Helixi78 – 803
    Helixi84 – 9613
    Helixi107 – 11711
    Helixi121 – 13010
    Beta strandi137 – 1393
    Helixi141 – 15515

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1E7DX-ray2.80A/B1-157[»]
    1E7LX-ray1.32A/B1-157[»]
    1EN7X-ray2.40A/B1-157[»]
    2QNCX-ray3.10A/B1-157[»]
    2QNFX-ray3.00A/B1-157[»]
    ProteinModelPortaliP13340.
    SMRiP13340. Positions 1-157.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP13340.

    Family & Domainsi

    Family and domain databases

    InterProiIPR004211. Endonuclease_7.
    IPR015208. T4_recomb_endonuclease_dimer.
    [Graphical view]
    PfamiPF09124. Endonuc-dimeris. 1 hit.
    PF02945. Endonuclease_7. 1 hit.
    [Graphical view]
    SUPFAMiSSF68918. SSF68918. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative initiation. Align

    Isoform Long (identifier: P13340-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MLLTGKLYKE EKQKFYDAQN GKCLICQREL NPDVQANHLD HDHELNGPKA    50
    GKVRGLLCNL CNAAEGQMKH KFNRSGLKGQ GVDYLEWLEN LLTYLKSDYT 100
    QNNIHPNFVG DKSKEFSRLG KEEMMAEMLQ RGFEYNESDT KTQLIASFKK 150
    QLRKSLK 157
    Length:157
    Mass (Da):18,144
    Last modified:January 1, 1990 - v1
    Checksum:i86233D2A7FC0F9A8
    GO
    Isoform Short (identifier: P13340-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-52: Missing.
         53-53: V → M

    Show »
    Length:105
    Mass (Da):12,213
    Checksum:iB2EDCD7FA9B65546
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 5252Missing in isoform Short. CuratedVSP_018678Add
    BLAST
    Alternative sequencei53 – 531V → M in isoform Short. CuratedVSP_018986

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X12629 Genomic DNA. Translation: CAA31148.1.
    X12629 Genomic DNA. Translation: CAA31149.1.
    Y00122 Genomic DNA. Translation: CAA68307.1.
    AF158101 Genomic DNA. Translation: AAD42477.1.
    AF158101 Genomic DNA. Translation: AAD42687.2.
    PIRiS01906. ZNBPT9.
    RefSeqiNP_049692.1. NC_000866.4. [P13340-1]
    NP_049693.2. NC_000866.4. [P13340-2]

    Genome annotation databases

    GeneIDi1258702.
    1258772.

    Keywords - Coding sequence diversityi

    Alternative initiation

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X12629 Genomic DNA. Translation: CAA31148.1 .
    X12629 Genomic DNA. Translation: CAA31149.1 .
    Y00122 Genomic DNA. Translation: CAA68307.1 .
    AF158101 Genomic DNA. Translation: AAD42477.1 .
    AF158101 Genomic DNA. Translation: AAD42687.2 .
    PIRi S01906. ZNBPT9.
    RefSeqi NP_049692.1. NC_000866.4. [P13340-1 ]
    NP_049693.2. NC_000866.4. [P13340-2 ]

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1E7D X-ray 2.80 A/B 1-157 [» ]
    1E7L X-ray 1.32 A/B 1-157 [» ]
    1EN7 X-ray 2.40 A/B 1-157 [» ]
    2QNC X-ray 3.10 A/B 1-157 [» ]
    2QNF X-ray 3.00 A/B 1-157 [» ]
    ProteinModelPortali P13340.
    SMRi P13340. Positions 1-157.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-60245N.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 1258702.
    1258772.

    Enzyme and pathway databases

    BRENDAi 3.1.22.4. 732.

    Miscellaneous databases

    EvolutionaryTracei P13340.

    Family and domain databases

    InterProi IPR004211. Endonuclease_7.
    IPR015208. T4_recomb_endonuclease_dimer.
    [Graphical view ]
    Pfami PF09124. Endonuc-dimeris. 1 hit.
    PF02945. Endonuclease_7. 1 hit.
    [Graphical view ]
    SUPFAMi SSF68918. SSF68918. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence and primary structures of gene products coded for by the T4 genome between map positions 48.266 kb and 39.166 kb."
      Tomaschewski J., Rueger W.
      Nucleic Acids Res. 15:3632-3633(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: C.
    2. "Regulation of two nested proteins from gene 49 (recombination endonuclease VII) and of a lambda RexA-like protein of bacteriophage T4."
      Barth K.A., Powell D., Trupin M., Mosig G.
      Genetics 120:329-343(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE INITIATION.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "Large-scale preparation of T4 endonuclease VII from over-expressing bacteria."
      Kosak H.G., Kemper B.W.
      Eur. J. Biochem. 194:779-784(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    5. "X-ray structure of T4 endonuclease VII: a DNA junction resolvase with a novel fold and unusual domain-swapped dimer architecture."
      Raaijmakers H., Vix O., Toro I., Golz S., Kemper B., Suck D.
      EMBO J. 18:1447-1458(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).

    Entry informationi

    Entry nameiEND7_BPT4
    AccessioniPrimary (citable) accession number: P13340
    Secondary accession number(s): Q38426, Q9T0V4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: January 1, 1990
    Last modified: October 1, 2014
    This is version 95 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references

    External Data

    Dasty 3