Recombination endonuclease VII - Enterobacteria phage T4

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Sequences

References

Cross-references

Entry information

Relevant documents

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Sequences

References

Cross-references

Entry information

Relevant documents

Molecule processing

Sites

Natural variations

Secondary structure

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Other

Preferred order of sections

Molecule processing

Sites

Natural variations

Secondary structure

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Other

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P13340 (END7_BPT4) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 90. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Protein names

Recommended name:
Recombination endonuclease VII
Short name=Endo VII
EC=3.1.-.-

Alternative name(s):
Protein Gp49

Gene names

Name:

Organism

Enterobacteria phage T4 (Bacteriophage T4) [Reference proteome]

Taxonomic identifier

10665 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Caudovirales › Myoviridae › Tevenvirinae › T4likevirus ›

Virus host

Escherichia coli [TaxID: 562]

Sequence length	157 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Function

Cleaves DNA cruciform and Y-structures as well as heteroduplex loops. Resolves Holliday junctions, recognizes a broad spectrum of DNA substrates ranging from branched DNAs to single base mismatches.

Cofactor

Binds 1 calcium ion per subunit.

Binds 1 zinc ion per subunit.

Subunit structure

Homodimer.

Keywords
Coding sequence diversity	Alternative initiation
Ligand	Calcium Metal-binding Zinc
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Molecular_function	endonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Chain

1 – 157

157

Recombination endonuclease VII

PRO_0000003326

Metal binding

Zinc

Metal binding

Zinc

Metal binding

Calcium

Metal binding

Zinc

Metal binding

Zinc

Metal binding

Calcium

Alternative sequence

1 – 52

Missing in isoform Short.

VSP_018678

Alternative sequence

V → M in isoform Short.

VSP_018986

157

Helix Strand Turn

Details...

Sequence		Length	Mass (Da)
Isoform Long [UniParc]. Last modified January 1, 1990. Version 1. Checksum: 86233D2A7FC0F9A8 Show »	FASTA	157	18,144
10 20 30 40 50 60 MLLTGKLYKE EKQKFYDAQN GKCLICQREL NPDVQANHLD HDHELNGPKA GKVRGLLCNL 70 80 90 100 110 120 CNAAEGQMKH KFNRSGLKGQ GVDYLEWLEN LLTYLKSDYT QNNIHPNFVG DKSKEFSRLG 130 140 150 KEEMMAEMLQ RGFEYNESDT KTQLIASFKK QLRKSLK « Hide
Isoform Short [UniParc]. Checksum: B2EDCD7FA9B65546 Show »	FASTA	105	12,213
10 20 30 40 50 60 MRGLLCNLCN AAEGQMKHKF NRSGLKGQGV DYLEWLENLL TYLKSDYTQN NIHPNFVGDK 70 80 90 100 SKEFSRLGKE EMMAEMLQRG FEYNESDTKT QLIASFKKQL RKSLK « Hide

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence and primary structures of gene products coded for by the T4 genome between map positions 48.266 kb and 39.166 kb." Tomaschewski J., Rueger W. Nucleic Acids Res. 15:3632-3633(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: C.
[2]	"Regulation of two nested proteins from gene 49 (recombination endonuclease VII) and of a lambda RexA-like protein of bacteriophage T4." Barth K.A., Powell D., Trupin M., Mosig G. Genetics 120:329-343(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE INITIATION.
[3]	"Bacteriophage T4 genome." Miller E.S., Kutter E., Mosig G., Arisaka F., Kunisawa T., Ruger W. Microbiol. Mol. Biol. Rev. 67:86-156(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"Large-scale preparation of T4 endonuclease VII from over-expressing bacteria." Kosak H.G., Kemper B.W. Eur. J. Biochem. 194:779-784(1990) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[5]	"X-ray structure of T4 endonuclease VII: a DNA junction resolvase with a novel fold and unusual domain-swapped dimer architecture." Raaijmakers H., Vix O., Toro I., Golz S., Kemper B., Suck D. EMBO J. 18:1447-1458(1999) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).
+	Additional computationally mapped references.

EMBL
GenBank
DDBJ

X12629 Genomic DNA. Translation: CAA31148.1.
X12629 Genomic DNA. Translation: CAA31149.1.
Y00122 Genomic DNA. Translation: CAA68307.1.
AF158101 Genomic DNA. Translation: AAD42477.1.
AF158101 Genomic DNA. Translation: AAD42687.2.

PIR

ZNBPT9. S01906.

RefSeq

NP_049692.1. NC_000866.4.
NP_049693.2. NC_000866.4.

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1E7D	X-ray	2.80	A/B	1-157	[»]
1E7L	X-ray	1.32	A/B	1-157	[»]
1EN7	X-ray	2.40	A/B	1-157	[»]
2QNC	X-ray	3.10	A/B	1-157	[»]
2QNF	X-ray	3.00	A/B	1-157	[»]

ProteinModelPortal

P13340.

SMR

P13340. Positions 1-157.

ModBase

Search...

StructuralBiologyKnowledgebase

Search...

GeneID

1258702.
1258772.

ProtClustDB

PHA2565.

BRENDA

3.1.22.4. 732.

InterPro

IPR004211. Endonuclease_7.
IPR015208. T4_recomb_endonuclease_dimer.
[Graphical view]

Pfam

PF09124. Endonuc-dimeris. 1 hit.
PF02945. Endonuclease_7. 1 hit.
[Graphical view]

SUPFAM

SSF68918. T4_recomb_endonuclease_dimer. 1 hit.

ProtoNet

Search...

EvolutionaryTrace

P13340.

Entry name

END7_BPT4

Accession

Primary (citable) accession number: P13340
Secondary accession number(s): Q38426, Q9T0V4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 1, 1990
Last modified:	May 1, 2013
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]

Isoform Long (identifier: P13340-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Short (identifier: P13340-2)

The sequence of this isoform differs from the canonical sequence as follows:
1-52: Missing.
53-53: V → M