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P13340

- END7_BPT4

UniProt

P13340 - END7_BPT4

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Protein

Recombination endonuclease VII

Gene

49

Organism
Enterobacteria phage T4 (Bacteriophage T4)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Cleaves DNA cruciform and Y-structures as well as heteroduplex loops. Resolves Holliday junctions, recognizes a broad spectrum of DNA substrates ranging from branched DNAs to single base mismatches.

Cofactori

Protein has several cofactor binding sites:
  • Ca2+Note: Binds 1 Ca(2+) ion per subunit.
  • Zn2+Note: Binds 1 zinc ion per subunit.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi23 – 231Zinc
Metal bindingi26 – 261Zinc
Metal bindingi40 – 401Calcium
Metal bindingi58 – 581Zinc
Metal bindingi61 – 611Zinc
Metal bindingi62 – 621Calcium

GO - Molecular functioni

  1. endonuclease activity Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.1.22.4. 732.

Names & Taxonomyi

Protein namesi
Recommended name:
Recombination endonuclease VII (EC:3.1.-.-)
Short name:
Endo VII
Alternative name(s):
Gene product 49
Short name:
gp49
Gene namesi
Name:49
OrganismiEnterobacteria phage T4 (Bacteriophage T4)
Taxonomic identifieri10665 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeTevenvirinaeT4likevirus
Virus hostiEscherichia coli [TaxID: 562]
ProteomesiUP000009087: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 157157Recombination endonuclease VIIPRO_0000003326Add
BLAST

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

DIPiDIP-60245N.

Structurei

Secondary structure

1
157
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi6 – 1813Combined sources
Turni19 – 213Combined sources
Turni24 – 263Combined sources
Helixi34 – 363Combined sources
Beta strandi37 – 415Combined sources
Beta strandi45 – 473Combined sources
Turni48 – 514Combined sources
Beta strandi52 – 576Combined sources
Helixi59 – 7416Combined sources
Beta strandi75 – 773Combined sources
Helixi78 – 803Combined sources
Helixi84 – 9613Combined sources
Helixi107 – 11711Combined sources
Helixi121 – 13010Combined sources
Beta strandi137 – 1393Combined sources
Helixi141 – 15515Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1E7DX-ray2.80A/B1-157[»]
1E7LX-ray1.32A/B1-157[»]
1EN7X-ray2.40A/B1-157[»]
2QNCX-ray3.10A/B1-157[»]
2QNFX-ray3.00A/B1-157[»]
ProteinModelPortaliP13340.
SMRiP13340. Positions 1-157.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP13340.

Family & Domainsi

Family and domain databases

InterProiIPR004211. Endonuclease_7.
IPR015208. T4_recomb_endonuclease_dimer.
[Graphical view]
PfamiPF09124. Endonuc-dimeris. 1 hit.
PF02945. Endonuclease_7. 1 hit.
[Graphical view]
SUPFAMiSSF68918. SSF68918. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative initiation. Align

Isoform Long (identifier: P13340-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLLTGKLYKE EKQKFYDAQN GKCLICQREL NPDVQANHLD HDHELNGPKA
60 70 80 90 100
GKVRGLLCNL CNAAEGQMKH KFNRSGLKGQ GVDYLEWLEN LLTYLKSDYT
110 120 130 140 150
QNNIHPNFVG DKSKEFSRLG KEEMMAEMLQ RGFEYNESDT KTQLIASFKK

QLRKSLK
Length:157
Mass (Da):18,144
Last modified:January 1, 1990 - v1
Checksum:i86233D2A7FC0F9A8
GO
Isoform Short (identifier: P13340-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-52: Missing.
     53-53: V → M

Show »
Length:105
Mass (Da):12,213
Checksum:iB2EDCD7FA9B65546
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 5252Missing in isoform Short. CuratedVSP_018678Add
BLAST
Alternative sequencei53 – 531V → M in isoform Short. CuratedVSP_018986

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X12629 Genomic DNA. Translation: CAA31148.1.
X12629 Genomic DNA. Translation: CAA31149.1.
Y00122 Genomic DNA. Translation: CAA68307.1.
AF158101 Genomic DNA. Translation: AAD42477.1.
AF158101 Genomic DNA. Translation: AAD42687.2.
PIRiS01906. ZNBPT9.
RefSeqiNP_049692.1. NC_000866.4. [P13340-1]
NP_049693.2. NC_000866.4. [P13340-2]

Genome annotation databases

GeneIDi1258702.
1258772.

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X12629 Genomic DNA. Translation: CAA31148.1 .
X12629 Genomic DNA. Translation: CAA31149.1 .
Y00122 Genomic DNA. Translation: CAA68307.1 .
AF158101 Genomic DNA. Translation: AAD42477.1 .
AF158101 Genomic DNA. Translation: AAD42687.2 .
PIRi S01906. ZNBPT9.
RefSeqi NP_049692.1. NC_000866.4. [P13340-1 ]
NP_049693.2. NC_000866.4. [P13340-2 ]

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1E7D X-ray 2.80 A/B 1-157 [» ]
1E7L X-ray 1.32 A/B 1-157 [» ]
1EN7 X-ray 2.40 A/B 1-157 [» ]
2QNC X-ray 3.10 A/B 1-157 [» ]
2QNF X-ray 3.00 A/B 1-157 [» ]
ProteinModelPortali P13340.
SMRi P13340. Positions 1-157.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-60245N.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 1258702.
1258772.

Enzyme and pathway databases

BRENDAi 3.1.22.4. 732.

Miscellaneous databases

EvolutionaryTracei P13340.

Family and domain databases

InterProi IPR004211. Endonuclease_7.
IPR015208. T4_recomb_endonuclease_dimer.
[Graphical view ]
Pfami PF09124. Endonuc-dimeris. 1 hit.
PF02945. Endonuclease_7. 1 hit.
[Graphical view ]
SUPFAMi SSF68918. SSF68918. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence and primary structures of gene products coded for by the T4 genome between map positions 48.266 kb and 39.166 kb."
    Tomaschewski J., Rueger W.
    Nucleic Acids Res. 15:3632-3633(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: C.
  2. "Regulation of two nested proteins from gene 49 (recombination endonuclease VII) and of a lambda RexA-like protein of bacteriophage T4."
    Barth K.A., Powell D., Trupin M., Mosig G.
    Genetics 120:329-343(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE INITIATION.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "Large-scale preparation of T4 endonuclease VII from over-expressing bacteria."
    Kosak H.G., Kemper B.W.
    Eur. J. Biochem. 194:779-784(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  5. "X-ray structure of T4 endonuclease VII: a DNA junction resolvase with a novel fold and unusual domain-swapped dimer architecture."
    Raaijmakers H., Vix O., Toro I., Golz S., Kemper B., Suck D.
    EMBO J. 18:1447-1458(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).

Entry informationi

Entry nameiEND7_BPT4
AccessioniPrimary (citable) accession number: P13340
Secondary accession number(s): Q38426, Q9T0V4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: November 26, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

External Data

Dasty 3