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Protein

Portal protein gp20

Gene

20

Organism
Enterobacteria phage T4 (Bacteriophage T4)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Forms the portal vertex of the capsid (PubMed:2685327, PubMed:24126213). This portal plays critical roles in head assembly, genome packaging, neck/tail attachment, and genome ejection. The portal protein multimerizes as a single ring-shaped homododecamer arranged around a central channel. Binds to the terminase subunits to form the packaging machine. Attaches to the host inner membrane most likely through interaction with host yidC and forms together with chaperone gp40 an initiator complex to form the prohead.Curated2 Publications

GO - Biological processi

Keywordsi

Molecular functionViral contractile tail ejection system
Biological processViral capsid assembly, Viral genome ejection through host cell envelope, Viral genome packaging, Viral penetration into host cytoplasm, Virus entry into host cell, Virus exit from host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Portal protein gp20
Alternative name(s):
Gene product 20
Short name:
gp20
Gene namesi
Name:20
OrganismiEnterobacteria phage T4 (Bacteriophage T4)
Taxonomic identifieri10665 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeTevenvirinaeT4virus
Virus hostiEscherichia coli [TaxID: 562]
Proteomesi
  • UP000009087 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

  • host cell plasma membrane Source: UniProtKB-SubCell
  • membrane Source: UniProtKB-KW
  • viral capsid Source: UniProtKB-KW
  • virion Source: CACAO

Keywords - Cellular componenti

Capsid protein, Host cell inner membrane, Host cell membrane, Host membrane, Membrane, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001650101 – 524Portal protein gp20Add BLAST524

Interactioni

Subunit structurei

Homododecamer. Interacts with the large terminase subunit gp17.1 Publication

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3JA7electron microscopy3.63A/B/C/D/E/F/G/H/I/J/K/L74-524[»]
SMRiP13334.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the T4likevirus portal protein family.Curated

Phylogenomic databases

OrthoDBiVOG0900006D.

Family and domain databases

InterProiView protein in InterPro
IPR010823. CAP_Gp20.
PfamiView protein in Pfam
PF07230. Peptidase_S80. 1 hit.

Sequencei

Sequence statusi: Complete.

P13334-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFNVLSLFA PWAKMDERNF KDQEKEDLVS ITAPKLDDGA REFEVSSNEA
60 70 80 90 100
ASPYNAAFQT IFGSYEPGMK TTRELIDTYR NLMNNYEVDN AVSEIVSDAI
110 120 130 140 150
VYEDDTEVVA LNLDKSKFSP KIKNMMLDEF SDVLNHLSFQ RKGSDHFRRW
160 170 180 190 200
YVDSRIFFHK IIDPKRPKEG IKELRRLDPR QVQYVREIIT ETEAGTKIVK
210 220 230 240 250
GYKEYFIYDT AHESYACDGR MYEAGTKIKI PKAAVVYAHS GLVDCCGKNI
260 270 280 290 300
IGYLHRAVKP ANQLKLLEDA VVIYRITRAP DRRVWYVDTG NMPARKAAEH
310 320 330 340 350
MQHVMNTMKN RVVYDASTGK IKNQQHNMSM TEDYWLQRRD GKAVTEVDTL
360 370 380 390 400
PGADNTGNME DIRWFRQALY MALRVPLSRI PQDQQGGVMF DSGTSITRDE
410 420 430 440 450
LTFAKFIREL QHKFEEVFLD PLKTNLLLKG IITEDEWNDE INNIKIEFHR
460 470 480 490 500
DSYFAELKEA EILERRINML TMAEPFIGKY ISHRTAMKDI LQMTDEEIEQ
510 520
EAKQIEEESK EARFQDPDQE QEDF
Length:524
Mass (Da):61,032
Last modified:December 19, 2001 - v2
Checksum:i1AF808425CBA84E4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti125M → I in CAA34190 (PubMed:2798102).Curated1
Sequence conflicti141R → Q in CAA34190 (PubMed:2798102).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X16055 Genomic DNA. Translation: CAA34190.1.
AF158101 Genomic DNA. Translation: AAD42425.1.
M19085 Genomic DNA. Translation: AAA32543.1.
PIRiJU0161. VHBP20.
RefSeqiNP_049782.1. NC_000866.4.

Genome annotation databases

GeneIDi1258582.
KEGGivg:1258582.

Similar proteinsi

Entry informationi

Entry nameiPORTL_BPT4
AccessioniPrimary (citable) accession number: P13334
Secondary accession number(s): Q9T0U2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: December 19, 2001
Last modified: May 10, 2017
This is version 68 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families