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Protein

Ribonuclease H

Gene

rnh

Organism
Enterobacteria phage T4 (Bacteriophage T4)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Play essential roles in DNA replication by removing the RNA primers from lagging strand fragments. Exhibits 5'to 3' exonuclease activity on either RNA/DNA or DNA/DNA duplexes and endonuclease activity on either flap or fork DNA structures.2 Publications

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomonoester.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionEndonuclease, Exonuclease, Hydrolase, Nuclease
Biological processDNA replication

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease H (EC:3.1.26.4)
Short name:
RNase H
Gene namesi
Name:rnh
Synonyms:33.2, das
OrganismiEnterobacteria phage T4 (Bacteriophage T4)
Taxonomic identifieri10665 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeTevenvirinaeT4virus
Virus hostiEscherichia coli [TaxID: 562]
Proteomesi
  • UP000009087 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001649771 – 305Ribonuclease HAdd BLAST305

Structurei

Secondary structure

1305
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 8Combined sources3
Beta strandi15 – 19Combined sources5
Helixi20 – 31Combined sources12
Beta strandi34 – 36Combined sources3
Helixi40 – 60Combined sources21
Beta strandi65 – 69Combined sources5
Helixi78 – 82Combined sources5
Turni84 – 87Combined sources4
Helixi88 – 96Combined sources9
Helixi101 – 118Combined sources18
Beta strandi119 – 124Combined sources6
Helixi131 – 144Combined sources14
Beta strandi149 – 152Combined sources4
Helixi159 – 163Combined sources5
Beta strandi164 – 171Combined sources8
Turni172 – 175Combined sources4
Beta strandi176 – 178Combined sources3
Helixi185 – 195Combined sources11
Helixi198 – 200Combined sources3
Helixi211 – 214Combined sources4
Helixi226 – 234Combined sources9
Helixi236 – 238Combined sources3
Helixi239 – 242Combined sources4
Helixi245 – 258Combined sources14
Helixi260 – 262Combined sources3
Helixi265 – 276Combined sources12
Helixi285 – 292Combined sources8
Helixi298 – 304Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1TFRX-ray2.06A1-305[»]
2IHNX-ray3.00A1-305[»]
3H7IX-ray1.50A1-305[»]
3H8JX-ray1.80A1-305[»]
3H8SX-ray2.51A1-305[»]
3H8WX-ray2.80A1-305[»]
ProteinModelPortaliP13319
SMRiP13319
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP13319

Family & Domainsi

Phylogenomic databases

KOiK18950
OrthoDBiVOG090000FN

Family and domain databases

InterProiView protein in InterPro
IPR020046 5-3_exonucl_a-hlix_arch_N
IPR036279 5-3_exonuclease_C_sf
IPR002421 5-3_exonuclease_N
IPR038969 FEN_bact-like
IPR008918 HhH2
IPR029060 PIN-like_dom_sf
IPR036276 T4_RNaseH_C
PANTHERiPTHR42646 PTHR42646, 1 hit
PfamiView protein in Pfam
PF02739 5_3_exonuc_N, 1 hit
PF09293 RNaseH_C, 1 hit
SMARTiView protein in SMART
SM00475 53EXOc, 1 hit
SM00279 HhH2, 1 hit
SUPFAMiSSF47807 SSF47807, 1 hit
SSF88723 SSF88723, 1 hit

Sequencei

Sequence statusi: Complete.

P13319-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDLEMMLDED YKEGICLIDF SQIALSTALV NFPDKEKINL SMVRHLILNS
60 70 80 90 100
IKFNVKKAKT LGYTKIVLCI DNAKSGYWRR DFAYYYKKNR GKAREESTWD
110 120 130 140 150
WEGYFESSHK VIDELKAYMP YIVMDIDKYE ADDHIAVLVK KFSLEGHKIL
160 170 180 190 200
IISSDGDFTQ LHKYPNVKQW SPMHKKWVKI KSGSAEIDCM TKILKGDKKD
210 220 230 240 250
NVASVKVRSD FWFTRVEGER TPSMKTSIVE AIANDREQAK VLLTESEYNR
260 270 280 290 300
YKENLVLIDF DYIPDNIASN IVNYYNSYKL PPRGKIYSYF VKAGLSKLTN

SINEF
Length:305
Mass (Da):35,558
Last modified:January 1, 1990 - v1
Checksum:i7956ECC356C0EDD0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15818 Genomic DNA Translation: CAA33812.1
AF158101 Genomic DNA Translation: AAD42554.1
PIRiS05556
RefSeqiNP_049859.1, NC_000866.4

Genome annotation databases

GeneIDi1258577
KEGGivg:1258577

Similar proteinsi

Entry informationi

Entry nameiRNH_BPT4
AccessioniPrimary (citable) accession number: P13319
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: June 20, 2018
This is version 109 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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