P13300 (KITH_BPT4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidine kinase
EC=2.7.1.21

Gene names

Name:

TK

Organism

Enterobacteria phage T4 (Bacteriophage T4) [Reference proteome]

Taxonomic identifier

10665 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Caudovirales › Myoviridae › Tevenvirinae › T4likevirus › Enterobacteria phage T4 sensu lato

Virus host

Escherichia coli [TaxID: 562]

Protein attributes

Sequence length	193 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This thymidine kinase is one of the enzymes that catalyze DNA precursor synthesis. Although tk is a nonessential gene, some strains of host E.coli do not support the growth of phages that lack this gene.
Catalytic activity	ATP + thymidine = ADP + thymidine 5'-phosphate.
Sequence similarities	Belongs to the thymidine kinase family.

Ontologies

Keywords
Biological process	DNA synthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	host cell cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW thymidine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 193	193	Thymidine kinase		PRO_0000175058
Regions
	Nucleotide binding	9 – 16	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P13300 [UniParc]. Last modified January 1, 1990. Version 1. Checksum: 95D7BE1AEB9F2C49	FASTA	193	21,622
	10 20 30 40 50 60 MASLIFTYAA MNAGKSASLL IAAHNYKERG MSVLVLKPAI DTRDSVCEVV SRIGIKQEAN 70 80 90 100 110 120 IITDDMDIFE FYKWAEAQKD IHCVFVDEAQ FLKTEQVHQL SRIVDTYNVP VMAYGLRTDF 130 140 150 160 170 180 AGKLFEGSKE LLAIADKLIE LKAVCHCGKK AIMTARLMED GTPVKEGNQI CIGDEIYVSL 190 CRKHWNELTK KLG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence and analysis of the 58.3 to 65.5-kb early region of bacteriophage T4." Valerie K., Stevens J., Lynch M., Henderson E.E., de Riel J.K. Nucleic Acids Res. 14:8637-8654(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Bacteriophage T4 genome." Miller E.S., Kutter E., Mosig G., Arisaka F., Kunisawa T., Ruger W. Microbiol. Mol. Biol. Rev. 67:86-156(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X04567 Genomic DNA. Translation: CAA28234.1. AF158101 Genomic DNA. Translation: AAD42663.1.
PIR	KIBPT4. JF0016.
RefSeq	NP_049719.1. NC_000866.4.
3D structure databases
ProteinModelPortal	P13300.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1258712.
Phylogenomic databases
ProtClustDB	CLSP2343322.
Family and domain databases
InterPro	IPR001267. Thymidine_kinase. IPR020633. Thymidine_kinase_CS. IPR020634. Thymidine_kinase_subgr. [Graphical view]
PANTHER	PTHR11441. PTHR11441. 1 hit.
Pfam	PF00265. TK. 1 hit. [Graphical view]
PIRSF	PIRSF035805. TK_cell. 1 hit.
PROSITE	PS00603. TK_CELLULAR_TYPE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KITH_BPT4

Accession

Primary (citable) accession number: P13300

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 1, 1990
Last modified:	May 1, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program