P13299 (TEV1_BPT4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Intron-associated endonuclease 1
EC=3.1.-.-

Alternative name(s):
I-TevI
IRF protein

Gene names

Name:

ITEVIR

Organism

Enterobacteria phage T4 (Bacteriophage T4) [Reference proteome]

Taxonomic identifier

10665 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Caudovirales › Myoviridae › Tevenvirinae › T4likevirus › Enterobacteria phage T4 sensu lato

Virus host

Escherichia coli [TaxID: 562]

Protein attributes

Sequence length	245 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This endonuclease is specific to the thymidylate synthase (td) gene splice junction and is involved in intron homing.
Cofactor	Magnesium.
Sequence similarities	Contains 1 GIY-YIG domain. To endonucleases of group I introns of fungi and phage.

Ontologies

Keywords
Biological process	Intron homing
Ligand	Magnesium
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	intron homing Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Molecular_function	endonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 245

245

Intron-associated endonuclease 1

PRO_0000192792

Regions

Domain

1 – 88

88

GIY-YIG

Secondary structure

1

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245

Helix Strand Turn

Details...

Beta strand

4 – 10

7

Turn

11 – 13

3

Beta strand

16 – 23

8

Helix

24 – 37

14

Helix

43 – 52

10

Beta strand

56 – 63

8

Helix

68 – 81

14

Turn

82 – 86

5

Beta strand

87 – 89

3

Beta strand

154 – 156

3

Turn

165 – 167

3

Helix

172 – 174

3

Turn

176 – 179

4

Helix

184 – 194

11

Beta strand

205 – 207

3

Beta strand

210 – 214

5

Helix

215 – 222

8

Helix

226 – 234

9

Beta strand

240 – 242

3

Sequences

	Sequence		Length	Mass (Da)
	P13299 [UniParc]. Last modified July 15, 1999. Version 2. Checksum: 29DF2ACF08ECD64C	FASTA	245	28,232
	10 20 30 40 50 60 MKSGIYQIKN TLNNKVYVGS AKDFEKRWKR HFKDLEKGCH SSIKLQRSFN KHGNVFECSI 70 80 90 100 110 120 LEEIPYEKDL IIERENFWIK ELNSKINGYN IADATFGDTC STHPLKEEII KKRSETVKAK 130 140 150 160 170 180 MLKLGPDGRK ALYSKPGSKN GRWNPETHKF CKCGVRIQTS AYTCSKCRNR SGENNSFFNH 190 200 210 220 230 240 KHSDITKSKI SEKMKGKKPS NIKKISCDGV IFDCAADAAR HFKISSGLVT YRVKSDKWNW FYINA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the intron in the phage T4 thymidylate synthase gene and evidence for its self-excision from the primary transcript." Chu F.K., Maley G.F., West D.K., Belfort M., Maley F. Cell 45:157-166(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ALC4.
[2]	Chu F.K. Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 67.
[3]	"Bacteriophage T4 genome." Miller E.S., Kutter E., Mosig G., Arisaka F., Kunisawa T., Ruger W. Microbiol. Mol. Biol. Rev. 67:86-156(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"Evidence that the intron open reading frame of the phage T4 td gene encodes a specific endonuclease." West D.K., Changchien L.-M., Maley G.F., Maley F. J. Biol. Chem. 264:10343-10346(1989) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION OF PROTEIN.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M12742 Genomic DNA. Translation: AAC12817.1.
AF158101 Genomic DNA. Translation: AAD42521.3.

PIR

T10130.

RefSeq

NP_049849.3. NC_000866.4.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1I3J	X-ray	2.20	A	130-245	[»]
1LN0	X-ray	2.00	A/B	1-97	[»]
1MK0	X-ray	1.60	A	1-97	[»]
1T2T	X-ray	2.50	A	130-245	[»]

ProteinModelPortal

P13299.

SMR

P13299. Positions 1-97, 149-244.

ModBase

Search...

Protein family/group databases

REBASE

2625. I-TevI.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

1258722.

Family and domain databases

Gene3D

3.40.1440.10. 1 hit.

InterPro

IPR027299. GIY-YIG_dom.
IPR000305. GIY-YIG_SF.
IPR006350. Intron_endoG1.
IPR003611. Intron_nuc_2.
[Graphical view]

Pfam

PF01541. GIY-YIG. 1 hit.
PF07460. NUMOD3. 2 hits.
[Graphical view]

SMART

SM00465. GIYc. 1 hit.
SM00496. IENR2. 2 hits.
[Graphical view]

SUPFAM

SSF82771. UvrC_N. 1 hit.

TIGRFAMs

TIGR01453. grpIintron_endo. 1 hit.

PROSITE

PS50164. GIY_YIG. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P13299.

Entry information

Entry name

TEV1_BPT4

Accession

Primary (citable) accession number: P13299
Secondary accession number(s): Q9T0T3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	July 15, 1999
Last modified:	May 1, 2013
This is version 92 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program