Orotate phosphoribosyltransferase 1 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P13298 (PYRE_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 114. History...

Clusters with 100%, 90%, 50% identity |

Documents (5) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Orotate phosphoribosyltransferase 1
Short name=OPRT 1
Short name=OPRTase 1
EC=2.4.2.10

Gene names

Name:	URA5
Synonyms:	PYR5
Ordered Locus Names:	YML106W
ORF Names:	YM8339.13

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	226 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) By similarity.
Catalytic activity	Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2.
Subunit structure	Homodimer By similarity.
Miscellaneous	There are two genes coding for OPRT in yeast. Present with 39300 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Molecular function	Glycosyltransferase Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from mutant phenotype. Source: SGD pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW pyrimidine ribonucleoside biosynthetic process Inferred from direct assay. Source: SGD
Cellular component	cytoplasm Inferred from direct assay. Source: SGD nucleus Inferred from direct assay. Source: SGD
Molecular function	orotate phosphoribosyltransferase activity Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 226

226

Orotate phosphoribosyltransferase 1

PRO_0000110804

Regions

Region

38 – 39

Orotate binding By similarity

Region

76 – 77

5-phosphoribose 1-diphosphate binding By similarity

Region

132 – 140

5-phosphoribose 1-diphosphate binding By similarity

Sites

Binding site

5-phosphoribose 1-diphosphate By similarity

Binding site

106

5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity

Binding site

107

5-phosphoribose 1-diphosphate By similarity

Binding site

110

5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity

Binding site

112

5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity

Binding site

136

Orotate By similarity

Binding site

164

Orotate By similarity

Experimental info

Sequence conflict

150

N → S in CAA32901. Ref.1

Secondary structure

226

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P13298 [UniParc].

Last modified February 1, 1996. Version 2.
Checksum: ECF3A01EB35BC03F
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FASTA

226

24,664

        10         20         30         40         50         60 
MPIMLEDYQK NFLELAIECQ ALRFGSFKLK SGRESPYFFN LGLFNTGKLL SNLATAYAIA 

        70         80         90        100        110        120 
IIQSDLKFDV IFGPAYKGIP LAAIVCVKLA EIGGSKFQNI QYAFNRKEAK DHGEGGIIVG 

       130        140        150        160        170        180 
SALENKRILI IDDVMTAGTA INEAFEIISN AKGQVVGSII ALDRQEVVST DDKEGLSATQ 

       190        200        210        220 
TVSKKYGIPV LSIVSLIHII TYLEGRITAE EKSKIEQYLQ TYGASA

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure and expression of the URA5 gene of Saccharomyces cerevisiae." de Montigny J., Belarbi A., Hubert J.-C., Lacroute F. Mol. Gen. Genet. 215:455-462(1989) [PubMed: 2651891] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	Stirling C.J. Submitted (FEB-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 13-226.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X14795 Genomic DNA. Translation: CAA32901.1.
Z49210 Genomic DNA. Translation: CAA89112.1.
AY693160 Genomic DNA. Translation: AAT93179.1.
X65783 Genomic DNA. Translation: CAA46665.1.
BK006946 Genomic DNA. Translation: DAA09792.1.

PIR

XJBY5. S53966.

RefSeq

NP_013601.1. NM_001182468.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2PRY	X-ray	2.35	A	1-226	[»]
2PRZ	X-ray	1.90	A/B/C/D	1-226	[»]
2PS1	X-ray	1.75	A/B	1-226	[»]

ProteinModelPortal

P13298.

SMR

P13298. Positions 3-226.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-4859N.

IntAct

P13298. 5 interactions.

MINT

MINT-548974.

STRING

P13298.

Proteomic databases

PeptideAtlas

P13298.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YML106W; YML106W; YML106W.

GeneID

854865.

KEGG

sce:YML106W.

NMPDR

fig|4932.3.peg.4637.

Organism-specific databases

CYGD

YML106w.

SGD

S000004574. URA5.

Phylogenomic databases

eggNOG

fuNOG08981.

GeneTree

EFGT00050000003681.

HOGENOM

HBG404341.

OMA

FAFNRKE.

OrthoDB

EOG4D566P.

Gene expression databases

ArrayExpress

P13298.

Genevestigator

P13298.

GermOnline

YML106W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR004467. Or_phspho_trans_clade-1.
IPR023031. Orotate_PribosylTferase.
IPR000836. PRibTrfase.
[Graphical view]

K00762.

Pfam

PF00156. Pribosyltran. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00336. PyrE. 1 hit.

PROSITE

PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

977789.

Entry information

Entry name

PYRE_YEAST

Accession

Primary (citable) accession number: P13298
Secondary accession number(s): D6W0H8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	February 1, 1996
Last modified:	December 14, 2011
This is version 114 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents