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P13297

- MSX1_MOUSE

UniProt

P13297 - MSX1_MOUSE

Protein

Homeobox protein MSX-1

Gene

Msx1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 147 (01 Oct 2014)
      Sequence version 4 (13 Nov 2013)
      Previous versions | rss
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    Functioni

    Acts as a transcriptional repressor. May play a role in limb-pattern formation. Acts in cranofacial development and specifically in odontogenesis.2 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi172 – 23160HomeoboxPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: MGI
    2. protein binding Source: IntAct
    3. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity Source: MGI
    4. RNA polymerase II regulatory region sequence-specific DNA binding Source: NTNU_SB
    5. RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription Source: NTNU_SB
    6. RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: MGI
    7. sequence-specific DNA binding Source: MGI

    GO - Biological processi

    1. activation of meiosis Source: MGI
    2. anterior/posterior pattern specification Source: MGI
    3. apoptotic nuclear changes Source: Ensembl
    4. BMP signaling pathway Source: MGI
    5. BMP signaling pathway involved in heart development Source: MGI
    6. bone morphogenesis Source: MGI
    7. cartilage morphogenesis Source: MGI
    8. cellular response to nicotine Source: Ensembl
    9. embryonic digit morphogenesis Source: MGI
    10. embryonic forelimb morphogenesis Source: MGI
    11. embryonic hindlimb morphogenesis Source: MGI
    12. embryonic limb morphogenesis Source: MGI
    13. embryonic nail plate morphogenesis Source: BHF-UCL
    14. epithelial to mesenchymal transition Source: UniProtKB
    15. epithelial to mesenchymal transition involved in endocardial cushion formation Source: MGI
    16. face morphogenesis Source: BHF-UCL
    17. forebrain development Source: MGI
    18. heart development Source: MGI
    19. heart morphogenesis Source: MGI
    20. in utero embryonic development Source: MGI
    21. mammary gland epithelium development Source: MGI
    22. mesenchymal cell proliferation Source: UniProtKB
    23. midbrain development Source: MGI
    24. middle ear morphogenesis Source: MGI
    25. muscle organ development Source: MGI
    26. negative regulation of apoptotic process Source: MGI
    27. negative regulation of cell growth Source: Ensembl
    28. negative regulation of cell proliferation Source: MGI
    29. negative regulation of DNA binding Source: MGI
    30. negative regulation of striated muscle cell differentiation Source: MGI
    31. negative regulation of transcription, DNA-templated Source: MGI
    32. negative regulation of transcription from RNA polymerase II promoter Source: NTNU_SB
    33. negative regulation of transcription regulatory region DNA binding Source: MGI
    34. odontogenesis Source: UniProtKB
    35. odontogenesis of dentin-containing tooth Source: BHF-UCL
    36. palate development Source: MGI
    37. pituitary gland development Source: Ensembl
    38. positive regulation of BMP signaling pathway Source: MGI
    39. positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator Source: Ensembl
    40. positive regulation of mesenchymal cell apoptotic process Source: MGI
    41. positive regulation of transcription from RNA polymerase II promoter Source: MGI
    42. protein localization to nucleus Source: Ensembl
    43. protein stabilization Source: Ensembl
    44. regulation of odontogenesis Source: MGI
    45. signal transduction involved in regulation of gene expression Source: MGI
    46. stem cell differentiation Source: MGI
    47. transcription from RNA polymerase II promoter Source: GOC

    Keywords - Molecular functioni

    Developmental protein, Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Homeobox protein MSX-1
    Alternative name(s):
    Homeobox protein Hox-7
    Hox-7.1
    Msh homeobox 1-like protein
    Gene namesi
    Name:Msx1
    Synonyms:Hox7, Hox7.1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 5

    Organism-specific databases

    MGIiMGI:97168. Msx1.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleus Source: MGI
    2. transcription factor complex Source: MGI

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi15 – 151K → R: Abolishes sumoylation. 1 Publication
    Mutagenesisi53 – 531K → R: Sumoylated. 1 Publication
    Mutagenesisi72 – 721K → R: Sumoylated. 1 Publication
    Mutagenesisi125 – 1251K → R: Sumoylated. 1 Publication
    Mutagenesisi133 – 1331K → R: Abolishes sumoylation. 1 Publication
    Mutagenesisi139 – 1391K → R: Sumoylated. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 303303Homeobox protein MSX-1PRO_0000049091Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Cross-linki15 – 15Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)
    Cross-linki133 – 133Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)

    Post-translational modificationi

    Sumoylated by PIAS1, desumoylated by SENP1.1 Publication

    Keywords - PTMi

    Isopeptide bond, Ubl conjugation

    Proteomic databases

    PRIDEiP13297.

    PTM databases

    PhosphoSiteiP13297.

    Expressioni

    Gene expression databases

    BgeeiP13297.
    CleanExiMM_MSX1.
    GenevestigatoriP13297.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Hist1h1bP432763EBI-903969,EBI-903960
    NdnP252332EBI-903969,EBI-1801080

    Protein-protein interaction databases

    IntActiP13297. 5 interactions.
    STRINGi10090.ENSMUSP00000058354.

    Structurei

    Secondary structure

    1
    303
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi181 – 19313
    Helixi199 – 20810
    Helixi213 – 22917

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1IG7X-ray2.20A173-230[»]
    ProteinModelPortaliP13297.
    SMRiP13297. Positions 173-230.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP13297.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the Msh homeobox family.Curated
    Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Homeobox

    Phylogenomic databases

    eggNOGiNOG298790.
    GeneTreeiENSGT00750000117446.
    HOVERGENiHBG005205.
    InParanoidiP13297.
    KOiK09341.
    OMAiEKQERTP.
    OrthoDBiEOG70GMGK.
    PhylomeDBiP13297.
    TreeFamiTF350699.

    Family and domain databases

    Gene3Di1.10.10.60. 1 hit.
    InterProiIPR017970. Homeobox_CS.
    IPR001356. Homeobox_dom.
    IPR020479. Homeobox_metazoa.
    IPR009057. Homeodomain-like.
    [Graphical view]
    PfamiPF00046. Homeobox. 1 hit.
    [Graphical view]
    PRINTSiPR00024. HOMEOBOX.
    SMARTiSM00389. HOX. 1 hit.
    [Graphical view]
    SUPFAMiSSF46689. SSF46689. 1 hit.
    PROSITEiPS00027. HOMEOBOX_1. 1 hit.
    PS50071. HOMEOBOX_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P13297-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAPAAAMTSL PLGVKVEDSA FAKPAGGGVG QAPGAAAATA TAMGTDEEGA    50
    KPKVPASLLP FSVEALMADH RKPGAKESVL VASEGAQAAG GSVQHLGTRP 100
    GSLGAPDAPS SPRPLGHFSV GGLLKLPEDA LVKAESPEKL DRTPWMQSPR 150
    FSPPPARRLS PPACTLRKHK TNRKPRTPFT TAQLLALERK FRQKQYLSIA 200
    ERAEFSSSLS LTETQVKIWF QNRRAKAKRL QEAELEKLKM AAKPMLPPAA 250
    FGLSFPLGGP AAVAAAAGAS LYSASGPFQR AALPVAPVGL YTAHVGYSMY 300
    HLT 303
    Length:303
    Mass (Da):31,672
    Last modified:November 13, 2013 - v4
    Checksum:i8E07FF038AEF0EDF
    GO

    Sequence cautioni

    The sequence CAA32871.1 differs from that shown. Reason: Frameshift at position 252.
    The sequence AAH16426.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
    The sequence CAA32871.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti263 – 2664Missing in CAA41944. (PubMed:1682128)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X14759 mRNA. Translation: CAA32871.1. Sequence problems.
    X59251 mRNA. Translation: CAA41944.1.
    AF308572 mRNA. Translation: AAG32466.1.
    AC114671 Genomic DNA. No translation available.
    CH466524 Genomic DNA. Translation: EDL37531.1.
    BC016426 mRNA. Translation: AAH16426.1. Different initiation.
    X14457 Genomic DNA. Translation: CAA32626.1.
    M38575 Genomic DNA. Translation: AAA37823.1.
    CCDSiCCDS19249.1.
    PIRiPS0411.
    S03636.
    S18813.
    RefSeqiNP_034965.2. NM_010835.2.
    UniGeneiMm.256509.

    Genome annotation databases

    EnsembliENSMUST00000063116; ENSMUSP00000058354; ENSMUSG00000048450.
    GeneIDi17701.
    KEGGimmu:17701.
    UCSCiuc008xfw.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X14759 mRNA. Translation: CAA32871.1 . Sequence problems.
    X59251 mRNA. Translation: CAA41944.1 .
    AF308572 mRNA. Translation: AAG32466.1 .
    AC114671 Genomic DNA. No translation available.
    CH466524 Genomic DNA. Translation: EDL37531.1 .
    BC016426 mRNA. Translation: AAH16426.1 . Different initiation.
    X14457 Genomic DNA. Translation: CAA32626.1 .
    M38575 Genomic DNA. Translation: AAA37823.1 .
    CCDSi CCDS19249.1.
    PIRi PS0411.
    S03636.
    S18813.
    RefSeqi NP_034965.2. NM_010835.2.
    UniGenei Mm.256509.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1IG7 X-ray 2.20 A 173-230 [» ]
    ProteinModelPortali P13297.
    SMRi P13297. Positions 173-230.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P13297. 5 interactions.
    STRINGi 10090.ENSMUSP00000058354.

    PTM databases

    PhosphoSitei P13297.

    Proteomic databases

    PRIDEi P13297.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000063116 ; ENSMUSP00000058354 ; ENSMUSG00000048450 .
    GeneIDi 17701.
    KEGGi mmu:17701.
    UCSCi uc008xfw.1. mouse.

    Organism-specific databases

    CTDi 4487.
    MGIi MGI:97168. Msx1.

    Phylogenomic databases

    eggNOGi NOG298790.
    GeneTreei ENSGT00750000117446.
    HOVERGENi HBG005205.
    InParanoidi P13297.
    KOi K09341.
    OMAi EKQERTP.
    OrthoDBi EOG70GMGK.
    PhylomeDBi P13297.
    TreeFami TF350699.

    Miscellaneous databases

    EvolutionaryTracei P13297.
    NextBioi 292294.
    PROi P13297.
    SOURCEi Search...

    Gene expression databases

    Bgeei P13297.
    CleanExi MM_MSX1.
    Genevestigatori P13297.

    Family and domain databases

    Gene3Di 1.10.10.60. 1 hit.
    InterProi IPR017970. Homeobox_CS.
    IPR001356. Homeobox_dom.
    IPR020479. Homeobox_metazoa.
    IPR009057. Homeodomain-like.
    [Graphical view ]
    Pfami PF00046. Homeobox. 1 hit.
    [Graphical view ]
    PRINTSi PR00024. HOMEOBOX.
    SMARTi SM00389. HOX. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46689. SSF46689. 1 hit.
    PROSITEi PS00027. HOMEOBOX_1. 1 hit.
    PS50071. HOMEOBOX_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A new family of mouse homeo box-containing genes: molecular structure, chromosomal location, and developmental expression of Hox-7.1."
      Hill R.E., Jones P.F., Rees A.R., Sime C.M., Justice M.J., Copeland N.G., Jenkins N.A., Graham E., Davidson D.R.
      Genes Dev. 3:26-37(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Embryo.
    2. "The Msh-like homeobox genes define domains in the developing vertebrate eye."
      Monaghan A.P., Davidson D.R., Sime C., Graham E., Baldock R., Bhattacharya S.S., Hill R.E.
      Development 112:1053-1061(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6.
      Tissue: Embryo.
    3. "Isolation of a mouse homeodomain protein MSX1 cDNA variant."
      Wu X.B., Liu Y.-H.
      Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6.
      Tissue: Embryo.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    5. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Salivary gland.
    7. "Hox-7, a mouse homeobox gene with a novel pattern of expression during embryogenesis."
      Robert B., Sassoon D., Jacq B., Gehring W.J., Buckingham M.
      EMBO J. 8:91-100(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 158-256.
    8. "Cloning and evolutionary analysis of msh-like homeobox genes from mouse, zebrafish and ascidian."
      Holland P.W.H.
      Gene 98:253-257(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 171-231.
    9. "Position-dependent expression of two related homeobox genes in developing vertebrate limbs."
      Davidson D.R., Crawley A., Hill R.E., Tickle C.
      Nature 352:429-431(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    10. "Transcriptional repression by Msx-1 does not require homeodomain DNA-binding sites."
      Catron K.M., Zhang H., Marshall S.C., Inostroza J.A., Wilson J.M., Abate C.
      Mol. Cell. Biol. 15:861-871(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    11. Cited for: SUMOYLATION AT LYS-15 AND LYS-133, MUTAGENESIS OF LYS-15; LYS-53; LYS-72; LYS-125; LYS-133 AND LYS-139.
    12. "Crystal structure of the Msx-1 homeodomain/DNA complex."
      Hovde S., Abate-Shen C., Geiger J.H.
      Biochemistry 40:12013-12021(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 173-230 IN COMPLEX WITH DNA.

    Entry informationi

    Entry nameiMSX1_MOUSE
    AccessioniPrimary (citable) accession number: P13297
    Secondary accession number(s): Q91W75, Q9ERF6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: November 13, 2013
    Last modified: October 1, 2014
    This is version 147 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Caution

    It is uncertain whether Met-1 or Met-7 is the initiator.Curated

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3