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P13297

- MSX1_MOUSE

UniProt

P13297 - MSX1_MOUSE

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Protein

Homeobox protein MSX-1

Gene

Msx1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Acts as a transcriptional repressor. May play a role in limb-pattern formation. Acts in cranofacial development and specifically in odontogenesis.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi172 – 23160HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: MGI
  2. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity Source: MGI
  3. RNA polymerase II regulatory region sequence-specific DNA binding Source: NTNU_SB
  4. RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription Source: NTNU_SB
  5. RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: MGI
  6. sequence-specific DNA binding Source: MGI

GO - Biological processi

  1. activation of meiosis Source: MGI
  2. anterior/posterior pattern specification Source: MGI
  3. BMP signaling pathway Source: MGI
  4. BMP signaling pathway involved in heart development Source: MGI
  5. bone morphogenesis Source: MGI
  6. cartilage morphogenesis Source: MGI
  7. cellular response to nicotine Source: Ensembl
  8. embryonic digit morphogenesis Source: MGI
  9. embryonic forelimb morphogenesis Source: MGI
  10. embryonic hindlimb morphogenesis Source: MGI
  11. embryonic limb morphogenesis Source: MGI
  12. embryonic nail plate morphogenesis Source: BHF-UCL
  13. epithelial to mesenchymal transition Source: UniProtKB
  14. epithelial to mesenchymal transition involved in endocardial cushion formation Source: MGI
  15. face morphogenesis Source: BHF-UCL
  16. forebrain development Source: MGI
  17. heart development Source: MGI
  18. heart morphogenesis Source: MGI
  19. in utero embryonic development Source: MGI
  20. mammary gland epithelium development Source: MGI
  21. mesenchymal cell proliferation Source: UniProtKB
  22. midbrain development Source: MGI
  23. middle ear morphogenesis Source: MGI
  24. muscle organ development Source: MGI
  25. negative regulation of apoptotic process Source: MGI
  26. negative regulation of cell growth Source: Ensembl
  27. negative regulation of cell proliferation Source: MGI
  28. negative regulation of DNA binding Source: MGI
  29. negative regulation of striated muscle cell differentiation Source: MGI
  30. negative regulation of transcription, DNA-templated Source: MGI
  31. negative regulation of transcription from RNA polymerase II promoter Source: NTNU_SB
  32. negative regulation of transcription regulatory region DNA binding Source: MGI
  33. odontogenesis Source: UniProtKB
  34. odontogenesis of dentin-containing tooth Source: BHF-UCL
  35. palate development Source: MGI
  36. pituitary gland development Source: Ensembl
  37. positive regulation of BMP signaling pathway Source: MGI
  38. positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator Source: Ensembl
  39. positive regulation of mesenchymal cell apoptotic process Source: MGI
  40. positive regulation of transcription from RNA polymerase II promoter Source: MGI
  41. protein localization to nucleus Source: Ensembl
  42. protein stabilization Source: Ensembl
  43. regulation of odontogenesis Source: MGI
  44. signal transduction involved in regulation of gene expression Source: MGI
  45. stem cell differentiation Source: MGI
  46. transcription from RNA polymerase II promoter Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Homeobox protein MSX-1
Alternative name(s):
Homeobox protein Hox-7
Hox-7.1
Msh homeobox 1-like protein
Gene namesi
Name:Msx1
Synonyms:Hox7, Hox7.1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 5

Organism-specific databases

MGIiMGI:97168. Msx1.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: Ensembl
  2. nucleus Source: MGI
  3. transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi15 – 151K → R: Abolishes sumoylation. 1 Publication
Mutagenesisi53 – 531K → R: Sumoylated. 1 Publication
Mutagenesisi72 – 721K → R: Sumoylated. 1 Publication
Mutagenesisi125 – 1251K → R: Sumoylated. 1 Publication
Mutagenesisi133 – 1331K → R: Abolishes sumoylation. 1 Publication
Mutagenesisi139 – 1391K → R: Sumoylated. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 303303Homeobox protein MSX-1PRO_0000049091Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki15 – 15Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)
Cross-linki133 – 133Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)

Post-translational modificationi

Sumoylated by PIAS1, desumoylated by SENP1.1 Publication

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PRIDEiP13297.

PTM databases

PhosphoSiteiP13297.

Expressioni

Gene expression databases

BgeeiP13297.
CleanExiMM_MSX1.
GenevestigatoriP13297.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
Hist1h1bP432763EBI-903969,EBI-903960
NdnP252332EBI-903969,EBI-1801080

Protein-protein interaction databases

IntActiP13297. 5 interactions.
STRINGi10090.ENSMUSP00000058354.

Structurei

Secondary structure

1
303
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi181 – 19313
Helixi199 – 20810
Helixi213 – 22917

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1IG7X-ray2.20A173-230[»]
ProteinModelPortaliP13297.
SMRiP13297. Positions 173-230.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP13297.

Family & Domainsi

Sequence similaritiesi

Belongs to the Msh homeobox family.Curated
Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiNOG298790.
GeneTreeiENSGT00760000118844.
HOVERGENiHBG005205.
InParanoidiP13297.
KOiK09341.
OMAiEKQERTP.
OrthoDBiEOG70GMGK.
PhylomeDBiP13297.
TreeFamiTF350699.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR020479. Homeobox_metazoa.
IPR009057. Homeodomain-like.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
[Graphical view]
PRINTSiPR00024. HOMEOBOX.
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P13297-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAPAAAMTSL PLGVKVEDSA FAKPAGGGVG QAPGAAAATA TAMGTDEEGA
60 70 80 90 100
KPKVPASLLP FSVEALMADH RKPGAKESVL VASEGAQAAG GSVQHLGTRP
110 120 130 140 150
GSLGAPDAPS SPRPLGHFSV GGLLKLPEDA LVKAESPEKL DRTPWMQSPR
160 170 180 190 200
FSPPPARRLS PPACTLRKHK TNRKPRTPFT TAQLLALERK FRQKQYLSIA
210 220 230 240 250
ERAEFSSSLS LTETQVKIWF QNRRAKAKRL QEAELEKLKM AAKPMLPPAA
260 270 280 290 300
FGLSFPLGGP AAVAAAAGAS LYSASGPFQR AALPVAPVGL YTAHVGYSMY

HLT
Length:303
Mass (Da):31,672
Last modified:November 13, 2013 - v4
Checksum:i8E07FF038AEF0EDF
GO

Sequence cautioni

The sequence CAA32871.1 differs from that shown. Reason: Frameshift at position 252.
The sequence AAH16426.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
The sequence CAA32871.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti263 – 2664Missing in CAA41944. (PubMed:1682128)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X14759 mRNA. Translation: CAA32871.1. Sequence problems.
X59251 mRNA. Translation: CAA41944.1.
AF308572 mRNA. Translation: AAG32466.1.
AC114671 Genomic DNA. No translation available.
CH466524 Genomic DNA. Translation: EDL37531.1.
BC016426 mRNA. Translation: AAH16426.1. Different initiation.
X14457 Genomic DNA. Translation: CAA32626.1.
M38575 Genomic DNA. Translation: AAA37823.1.
CCDSiCCDS19249.1.
PIRiPS0411.
S03636.
S18813.
RefSeqiNP_034965.2. NM_010835.2.
UniGeneiMm.256509.

Genome annotation databases

EnsembliENSMUST00000063116; ENSMUSP00000058354; ENSMUSG00000048450.
GeneIDi17701.
KEGGimmu:17701.
UCSCiuc008xfw.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X14759 mRNA. Translation: CAA32871.1 . Sequence problems.
X59251 mRNA. Translation: CAA41944.1 .
AF308572 mRNA. Translation: AAG32466.1 .
AC114671 Genomic DNA. No translation available.
CH466524 Genomic DNA. Translation: EDL37531.1 .
BC016426 mRNA. Translation: AAH16426.1 . Different initiation.
X14457 Genomic DNA. Translation: CAA32626.1 .
M38575 Genomic DNA. Translation: AAA37823.1 .
CCDSi CCDS19249.1.
PIRi PS0411.
S03636.
S18813.
RefSeqi NP_034965.2. NM_010835.2.
UniGenei Mm.256509.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1IG7 X-ray 2.20 A 173-230 [» ]
ProteinModelPortali P13297.
SMRi P13297. Positions 173-230.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P13297. 5 interactions.
STRINGi 10090.ENSMUSP00000058354.

PTM databases

PhosphoSitei P13297.

Proteomic databases

PRIDEi P13297.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000063116 ; ENSMUSP00000058354 ; ENSMUSG00000048450 .
GeneIDi 17701.
KEGGi mmu:17701.
UCSCi uc008xfw.1. mouse.

Organism-specific databases

CTDi 4487.
MGIi MGI:97168. Msx1.

Phylogenomic databases

eggNOGi NOG298790.
GeneTreei ENSGT00760000118844.
HOVERGENi HBG005205.
InParanoidi P13297.
KOi K09341.
OMAi EKQERTP.
OrthoDBi EOG70GMGK.
PhylomeDBi P13297.
TreeFami TF350699.

Miscellaneous databases

EvolutionaryTracei P13297.
NextBioi 292294.
PROi P13297.
SOURCEi Search...

Gene expression databases

Bgeei P13297.
CleanExi MM_MSX1.
Genevestigatori P13297.

Family and domain databases

Gene3Di 1.10.10.60. 1 hit.
InterProi IPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR020479. Homeobox_metazoa.
IPR009057. Homeodomain-like.
[Graphical view ]
Pfami PF00046. Homeobox. 1 hit.
[Graphical view ]
PRINTSi PR00024. HOMEOBOX.
SMARTi SM00389. HOX. 1 hit.
[Graphical view ]
SUPFAMi SSF46689. SSF46689. 1 hit.
PROSITEi PS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A new family of mouse homeo box-containing genes: molecular structure, chromosomal location, and developmental expression of Hox-7.1."
    Hill R.E., Jones P.F., Rees A.R., Sime C.M., Justice M.J., Copeland N.G., Jenkins N.A., Graham E., Davidson D.R.
    Genes Dev. 3:26-37(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Embryo.
  2. "The Msh-like homeobox genes define domains in the developing vertebrate eye."
    Monaghan A.P., Davidson D.R., Sime C., Graham E., Baldock R., Bhattacharya S.S., Hill R.E.
    Development 112:1053-1061(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
    Tissue: Embryo.
  3. "Isolation of a mouse homeodomain protein MSX1 cDNA variant."
    Wu X.B., Liu Y.-H.
    Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
    Tissue: Embryo.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  5. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Salivary gland.
  7. "Hox-7, a mouse homeobox gene with a novel pattern of expression during embryogenesis."
    Robert B., Sassoon D., Jacq B., Gehring W.J., Buckingham M.
    EMBO J. 8:91-100(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 158-256.
  8. "Cloning and evolutionary analysis of msh-like homeobox genes from mouse, zebrafish and ascidian."
    Holland P.W.H.
    Gene 98:253-257(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 171-231.
  9. "Position-dependent expression of two related homeobox genes in developing vertebrate limbs."
    Davidson D.R., Crawley A., Hill R.E., Tickle C.
    Nature 352:429-431(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. "Transcriptional repression by Msx-1 does not require homeodomain DNA-binding sites."
    Catron K.M., Zhang H., Marshall S.C., Inostroza J.A., Wilson J.M., Abate C.
    Mol. Cell. Biol. 15:861-871(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. Cited for: SUMOYLATION AT LYS-15 AND LYS-133, MUTAGENESIS OF LYS-15; LYS-53; LYS-72; LYS-125; LYS-133 AND LYS-139.
  12. "Crystal structure of the Msx-1 homeodomain/DNA complex."
    Hovde S., Abate-Shen C., Geiger J.H.
    Biochemistry 40:12013-12021(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 173-230 IN COMPLEX WITH DNA.

Entry informationi

Entry nameiMSX1_MOUSE
AccessioniPrimary (citable) accession number: P13297
Secondary accession number(s): Q91W75, Q9ERF6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: November 13, 2013
Last modified: October 29, 2014
This is version 148 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

It is uncertain whether Met-1 or Met-7 is the initiator.Curated

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3