ID   FIXK_RHIME              Reviewed;         211 AA.
AC   P13295;
DT   01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1990, sequence version 1.
DT   27-MAR-2024, entry version 135.
DE   RecName: Full=Nitrogen fixation regulation protein FixK;
GN   Name=fixK; OrderedLocusNames=RA0667; ORFNames=SMa1225;
OS   Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium
OS   meliloti).
OG   Plasmid pSymA (megaplasmid 1).
OC   Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX   NCBI_TaxID=266834;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2663474; DOI=10.1002/j.1460-2075.1989.tb03502.x;
RA   Batut J., Daveran-Mingot M.-L., David M., Jacobs J., Garnerone A.-M.,
RA   Kahn D.;
RT   "fixK, a gene homologous with fnr and crp from Escherichia coli, regulates
RT   nitrogen fixation genes both positively and negatively in Rhizobium
RT   meliloti.";
RL   EMBO J. 8:1279-1286(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1021;
RX   PubMed=11481432; DOI=10.1073/pnas.161294798;
RA   Barnett M.J., Fisher R.F., Jones T., Komp C., Abola A.P., Barloy-Hubler F.,
RA   Bowser L., Capela D., Galibert F., Gouzy J., Gurjal M., Hong A., Huizar L.,
RA   Hyman R.W., Kahn D., Kahn M.L., Kalman S., Keating D.H., Palm C.,
RA   Peck M.C., Surzycki R., Wells D.H., Yeh K.-C., Davis R.W., Federspiel N.A.,
RA   Long S.R.;
RT   "Nucleotide sequence and predicted functions of the entire Sinorhizobium
RT   meliloti pSymA megaplasmid.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:9883-9888(2001).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1021;
RX   PubMed=11474104; DOI=10.1126/science.1060966;
RA   Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F.,
RA   Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G.,
RA   Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P.,
RA   Cowie A., Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S.,
RA   Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I.,
RA   Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S.,
RA   Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C.,
RA   Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R.,
RA   Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H.,
RA   Wong K., Yeh K.-C., Batut J.;
RT   "The composite genome of the legume symbiont Sinorhizobium meliloti.";
RL   Science 293:668-672(2001).
CC   -!- FUNCTION: FixK is a protein that regulates nitrogen fixation genes both
CC       positively and negatively. FixK appears to repress its own expression
CC       and that of nifA. FixK may bind DNA at the FNR consensus binding site.
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DR   EMBL; X15079; CAA33183.1; -; Genomic_DNA.
DR   EMBL; Z21854; CAA79899.1; -; Genomic_DNA.
DR   EMBL; AE006469; AAK65325.1; -; Genomic_DNA.
DR   PIR; C95345; C95345.
DR   PIR; S04122; S04122.
DR   RefSeq; NP_435913.1; NC_003037.1.
DR   RefSeq; WP_010967646.1; NC_003037.1.
DR   AlphaFoldDB; P13295; -.
DR   SMR; P13295; -.
DR   EnsemblBacteria; AAK65325; AAK65325; SMa1225.
DR   GeneID; 61599435; -.
DR   KEGG; sme:SMa1225; -.
DR   PATRIC; fig|266834.11.peg.687; -.
DR   HOGENOM; CLU_075053_0_1_5; -.
DR   OrthoDB; 667966at2; -.
DR   Proteomes; UP000001976; Plasmid pSymA.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR   CDD; cd00038; CAP_ED; 1.
DR   CDD; cd00092; HTH_CRP; 1.
DR   Gene3D; 2.60.120.10; Jelly Rolls; 1.
DR   Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 1.
DR   InterPro; IPR000595; cNMP-bd_dom.
DR   InterPro; IPR018490; cNMP-bd_dom_sf.
DR   InterPro; IPR012318; HTH_CRP.
DR   InterPro; IPR014710; RmlC-like_jellyroll.
DR   InterPro; IPR018335; Tscrpt_reg_HTH_Crp-type_CS.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   PANTHER; PTHR24567; CRP FAMILY TRANSCRIPTIONAL REGULATORY PROTEIN; 1.
DR   PANTHER; PTHR24567:SF75; FUMARATE AND NITRATE REDUCTION REGULATORY PROTEIN; 1.
DR   Pfam; PF00027; cNMP_binding; 1.
DR   Pfam; PF13545; HTH_Crp_2; 1.
DR   PRINTS; PR00034; HTHCRP.
DR   SMART; SM00100; cNMP; 1.
DR   SMART; SM00419; HTH_CRP; 1.
DR   SUPFAM; SSF51206; cAMP-binding domain-like; 1.
DR   SUPFAM; SSF46785; Winged helix' DNA-binding domain; 1.
DR   PROSITE; PS50042; CNMP_BINDING_3; 1.
DR   PROSITE; PS00042; HTH_CRP_1; 1.
DR   PROSITE; PS51063; HTH_CRP_2; 1.
PE   4: Predicted;
KW   Activator; DNA-binding; Nitrogen fixation; Plasmid; Reference proteome;
KW   Repressor; Transcription; Transcription regulation.
FT   CHAIN           1..211
FT                   /note="Nitrogen fixation regulation protein FixK"
FT                   /id="PRO_0000100158"
FT   DOMAIN          134..204
FT                   /note="HTH crp-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00387"
FT   DNA_BIND        163..182
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00387"
SQ   SEQUENCE   211 AA;  23478 MW;  20357EE9974559EC CRC64;
     MYAAAQAKPQ SIEVEHLGPA PMSGPRLVAT YKPGREIYAQ GDLNDKCYQV STGAVRIYRL
     LSDGRRQVVS FHLPGEMFGF EAGSNHSFFA EAITETTLAI FGRRNMQERS RELLALALTG
     MARAQQHLLV IGRQCAVERI AAFLVDLCER QGGGRQLRLP MSRQDIADYL GLTIETVSRV
     VTKLKERSLI ALRDARTIDI MKPEALRSLC N
//