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Protein

Nitrogen fixation regulation protein FixK

Gene

fixK

Organism
Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

FixK is a protein that regulates nitrogen fixation genes both positively and negatively. FixK appears to repress its own expression and that of nifA. FixK may bind DNA at the FNR consensus binding site.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi163 – 18220H-T-H motifPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. sequence-specific DNA binding transcription factor activity Source: InterPro

GO - Biological processi

  1. nitrogen fixation Source: UniProtKB-KW
  2. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Nitrogen fixation, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciSMEL266834:GJF6-5675-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrogen fixation regulation protein FixK
Gene namesi
Name:fixK
Ordered Locus Names:RA0667
ORF Names:SMa1225
Encoded oniPlasmid pSymA (megaplasmid 1)0 Publication
OrganismiRhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Taxonomic identifieri266834 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
ProteomesiUP000001976: Plasmid pSymA

Subcellular locationi

GO - Cellular componenti

  1. intracellular Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 211211Nitrogen fixation regulation protein FixKPRO_0000100158Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi266834.SMa1225.

Structurei

3D structure databases

ProteinModelPortaliP13295.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini134 – 20471HTH crp-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 cyclic nucleotide-binding domain.PROSITE-ProRule annotation
Contains 1 HTH crp-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0664.
HOGENOMiHOG000186462.
KOiK15861.
OMAiHHFFAEA.
OrthoDBiEOG6FRCW4.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
2.60.120.10. 1 hit.
InterProiIPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR012318. HTH_CRP_2.
IPR014710. RmlC-like_jellyroll.
IPR001808. Tscrpt_reg_HTH_Crp.
IPR018335. Tscrpt_reg_HTH_Crp-type_CS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00027. cNMP_binding. 1 hit.
PF00325. Crp. 1 hit.
[Graphical view]
PRINTSiPR00034. HTHCRP.
SMARTiSM00100. cNMP. 1 hit.
SM00419. HTH_CRP. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 1 hit.
PROSITEiPS50042. CNMP_BINDING_3. 1 hit.
PS00042. HTH_CRP_1. 1 hit.
PS51063. HTH_CRP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P13295-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MYAAAQAKPQ SIEVEHLGPA PMSGPRLVAT YKPGREIYAQ GDLNDKCYQV
60 70 80 90 100
STGAVRIYRL LSDGRRQVVS FHLPGEMFGF EAGSNHSFFA EAITETTLAI
110 120 130 140 150
FGRRNMQERS RELLALALTG MARAQQHLLV IGRQCAVERI AAFLVDLCER
160 170 180 190 200
QGGGRQLRLP MSRQDIADYL GLTIETVSRV VTKLKERSLI ALRDARTIDI
210
MKPEALRSLC N
Length:211
Mass (Da):23,478
Last modified:January 1, 1990 - v1
Checksum:i20357EE9974559EC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15079 Genomic DNA. Translation: CAA33183.1.
Z21854 Genomic DNA. Translation: CAA79899.1.
AE006469 Genomic DNA. Translation: AAK65325.1.
PIRiC95345.
S04122.
RefSeqiNP_435913.1. NC_003037.1.
WP_010967646.1. NC_003037.1.

Genome annotation databases

EnsemblBacteriaiAAK65325; AAK65325; SMa1225.
GeneIDi1235703.
KEGGisme:SMa1225.
PATRICi23627816. VBISinMel96828_0687.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15079 Genomic DNA. Translation: CAA33183.1.
Z21854 Genomic DNA. Translation: CAA79899.1.
AE006469 Genomic DNA. Translation: AAK65325.1.
PIRiC95345.
S04122.
RefSeqiNP_435913.1. NC_003037.1.
WP_010967646.1. NC_003037.1.

3D structure databases

ProteinModelPortaliP13295.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi266834.SMa1225.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK65325; AAK65325; SMa1225.
GeneIDi1235703.
KEGGisme:SMa1225.
PATRICi23627816. VBISinMel96828_0687.

Phylogenomic databases

eggNOGiCOG0664.
HOGENOMiHOG000186462.
KOiK15861.
OMAiHHFFAEA.
OrthoDBiEOG6FRCW4.

Enzyme and pathway databases

BioCyciSMEL266834:GJF6-5675-MONOMER.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
2.60.120.10. 1 hit.
InterProiIPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR012318. HTH_CRP_2.
IPR014710. RmlC-like_jellyroll.
IPR001808. Tscrpt_reg_HTH_Crp.
IPR018335. Tscrpt_reg_HTH_Crp-type_CS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00027. cNMP_binding. 1 hit.
PF00325. Crp. 1 hit.
[Graphical view]
PRINTSiPR00034. HTHCRP.
SMARTiSM00100. cNMP. 1 hit.
SM00419. HTH_CRP. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 1 hit.
PROSITEiPS50042. CNMP_BINDING_3. 1 hit.
PS00042. HTH_CRP_1. 1 hit.
PS51063. HTH_CRP_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "fixK, a gene homologous with fnr and crp from Escherichia coli, regulates nitrogen fixation genes both positively and negatively in Rhizobium meliloti."
    Batut J., Daveran-Mingot M.-L., David M., Jacobs J., Garnerone A.-M., Kahn D.
    EMBO J. 8:1279-1286(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 1021.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 1021.

Entry informationi

Entry nameiFIXK_RHIME
AccessioniPrimary (citable) accession number: P13295
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: January 7, 2015
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Plasmid, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.