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P13295

- FIXK_RHIME

UniProt

P13295 - FIXK_RHIME

Protein

Nitrogen fixation regulation protein FixK

Gene

fixK

Organism
Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 1 (01 Jan 1990)
      Previous versions | rss
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    Functioni

    FixK is a protein that regulates nitrogen fixation genes both positively and negatively. FixK appears to repress its own expression and that of nifA. FixK may bind DNA at the FNR consensus binding site.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi163 – 18220H-T-H motifPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. sequence-specific DNA binding transcription factor activity Source: InterPro

    GO - Biological processi

    1. nitrogen fixation Source: UniProtKB-KW
    2. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator, Repressor

    Keywords - Biological processi

    Nitrogen fixation, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    BioCyciSMEL266834:GJF6-5675-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nitrogen fixation regulation protein FixK
    Gene namesi
    Name:fixK
    Ordered Locus Names:RA0667
    ORF Names:SMa1225
    Encoded oniPlasmid pSymA (megaplasmid 1)0 Publication
    OrganismiRhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
    Taxonomic identifieri266834 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
    ProteomesiUP000001976: Plasmid pSymA

    Subcellular locationi

    GO - Cellular componenti

    1. intracellular Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 211211Nitrogen fixation regulation protein FixKPRO_0000100158Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi266834.SMa1225.

    Structurei

    3D structure databases

    ProteinModelPortaliP13295.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini134 – 20471HTH crp-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 cyclic nucleotide-binding domain.PROSITE-ProRule annotation
    Contains 1 HTH crp-type DNA-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0664.
    HOGENOMiHOG000186462.
    KOiK15861.
    OMAiHIRILNI.
    OrthoDBiEOG6FRCW4.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    2.60.120.10. 1 hit.
    InterProiIPR018490. cNMP-bd-like.
    IPR000595. cNMP-bd_dom.
    IPR012318. HTH_CRP_2.
    IPR014710. RmlC-like_jellyroll.
    IPR001808. Tscrpt_reg_HTH_Crp.
    IPR018335. Tscrpt_reg_HTH_Crp-type_CS.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF00027. cNMP_binding. 1 hit.
    PF00325. Crp. 1 hit.
    [Graphical view]
    PRINTSiPR00034. HTHCRP.
    SMARTiSM00100. cNMP. 1 hit.
    SM00419. HTH_CRP. 1 hit.
    [Graphical view]
    SUPFAMiSSF51206. SSF51206. 1 hit.
    PROSITEiPS50042. CNMP_BINDING_3. 1 hit.
    PS00042. HTH_CRP_1. 1 hit.
    PS51063. HTH_CRP_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P13295-1 [UniParc]FASTAAdd to Basket

    « Hide

    MYAAAQAKPQ SIEVEHLGPA PMSGPRLVAT YKPGREIYAQ GDLNDKCYQV    50
    STGAVRIYRL LSDGRRQVVS FHLPGEMFGF EAGSNHSFFA EAITETTLAI 100
    FGRRNMQERS RELLALALTG MARAQQHLLV IGRQCAVERI AAFLVDLCER 150
    QGGGRQLRLP MSRQDIADYL GLTIETVSRV VTKLKERSLI ALRDARTIDI 200
    MKPEALRSLC N 211
    Length:211
    Mass (Da):23,478
    Last modified:January 1, 1990 - v1
    Checksum:i20357EE9974559EC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X15079 Genomic DNA. Translation: CAA33183.1.
    Z21854 Genomic DNA. Translation: CAA79899.1.
    AE006469 Genomic DNA. Translation: AAK65325.1.
    PIRiC95345.
    S04122.
    RefSeqiNP_435913.1. NC_003037.1.
    WP_010967646.1. NC_003037.1.

    Genome annotation databases

    EnsemblBacteriaiAAK65325; AAK65325; SMa1225.
    GeneIDi1235703.
    KEGGisme:SMa1225.
    PATRICi23627816. VBISinMel96828_0687.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X15079 Genomic DNA. Translation: CAA33183.1 .
    Z21854 Genomic DNA. Translation: CAA79899.1 .
    AE006469 Genomic DNA. Translation: AAK65325.1 .
    PIRi C95345.
    S04122.
    RefSeqi NP_435913.1. NC_003037.1.
    WP_010967646.1. NC_003037.1.

    3D structure databases

    ProteinModelPortali P13295.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 266834.SMa1225.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAK65325 ; AAK65325 ; SMa1225 .
    GeneIDi 1235703.
    KEGGi sme:SMa1225.
    PATRICi 23627816. VBISinMel96828_0687.

    Phylogenomic databases

    eggNOGi COG0664.
    HOGENOMi HOG000186462.
    KOi K15861.
    OMAi HIRILNI.
    OrthoDBi EOG6FRCW4.

    Enzyme and pathway databases

    BioCyci SMEL266834:GJF6-5675-MONOMER.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    2.60.120.10. 1 hit.
    InterProi IPR018490. cNMP-bd-like.
    IPR000595. cNMP-bd_dom.
    IPR012318. HTH_CRP_2.
    IPR014710. RmlC-like_jellyroll.
    IPR001808. Tscrpt_reg_HTH_Crp.
    IPR018335. Tscrpt_reg_HTH_Crp-type_CS.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF00027. cNMP_binding. 1 hit.
    PF00325. Crp. 1 hit.
    [Graphical view ]
    PRINTSi PR00034. HTHCRP.
    SMARTi SM00100. cNMP. 1 hit.
    SM00419. HTH_CRP. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51206. SSF51206. 1 hit.
    PROSITEi PS50042. CNMP_BINDING_3. 1 hit.
    PS00042. HTH_CRP_1. 1 hit.
    PS51063. HTH_CRP_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "fixK, a gene homologous with fnr and crp from Escherichia coli, regulates nitrogen fixation genes both positively and negatively in Rhizobium meliloti."
      Batut J., Daveran-Mingot M.-L., David M., Jacobs J., Garnerone A.-M., Kahn D.
      EMBO J. 8:1279-1286(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 1021.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 1021.

    Entry informationi

    Entry nameiFIXK_RHIME
    AccessioniPrimary (citable) accession number: P13295
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: January 1, 1990
    Last modified: October 1, 2014
    This is version 100 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Plasmid, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3