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P13286

- PHKG1_RAT

UniProt

P13286 - PHKG1_RAT

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Protein
Phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoform
Gene
Phkg1, Phkg
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic subunit of the phosphorylase b kinase (PHK), which mediates the neural and hormonal regulation of glycogen breakdown (glycogenolysis) by phosphorylating and thereby activating glycogen phosphorylase. In vitro, phosphorylates PYGM, TNNI3, MAPT/TAU, GAP43 and NRGN/RC3 By similarity.

Catalytic activityi

2 ATP + phosphorylase b = 2 ADP + phosphorylase a.
ATP + [tau protein] = ADP + [tau protein] phosphate.
ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei49 – 491ATP By similarity
Active sitei150 – 1501Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi26 – 349ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: RGD
  2. enzyme binding Source: RGD
  3. phosphorylase kinase activity Source: RGD
  4. tau-protein kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. glycogen biosynthetic process Source: InterPro
  2. glycogen metabolic process Source: RGD
  3. protein phosphorylation Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Muscle protein, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen metabolism

Keywords - Ligandi

ATP-binding, Calmodulin-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.19. 5301.
ReactomeiREACT_227999. Glycogen breakdown (glycogenolysis).

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoform (EC:2.7.11.19)
Alternative name(s):
Phosphorylase kinase subunit gamma-1
Serine/threonine-protein kinase PHKG1 (EC:2.7.11.1, EC:2.7.11.26)
Gene namesi
Name:Phkg1
Synonyms:Phkg
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 12

Organism-specific databases

RGDi3325. Phkg1.

Subcellular locationi

GO - Cellular componenti

  1. phosphorylase kinase complex Source: RGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 388387Phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoform
PRO_0000086511Add
BLAST

Proteomic databases

PaxDbiP13286.
PRIDEiP13286.

Expressioni

Gene expression databases

GenevestigatoriP13286.

Interactioni

Subunit structurei

Hexadecamer of 4 heterotetramers, each composed of alpha, beta, gamma, and delta subunits. Alpha (PHKA1 or PHKA2) and beta (PHKB) are regulatory subunits, gamma (PHKG1 or PHKG2) is the catalytic subunit, and delta is calmodulin.

Protein-protein interaction databases

BioGridi248008. 1 interaction.
STRINGi10116.ENSRNOP00000001222.

Structurei

3D structure databases

ProteinModelPortaliP13286.
SMRiP13286. Positions 12-292.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini20 – 288269Protein kinase
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni303 – 32725Calmodulin-binding (domain-N)
Add
BLAST
Regioni343 – 36725Calmodulin-binding (domain-C)
Add
BLAST

Domaini

The two calmodulin-binding domains appear to act in concert to bind a single molecule of calmodulin and are pseudosubstrate/autoinhibitory domains By similarity.

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00750000117716.
HOGENOMiHOG000233016.
HOVERGENiHBG106193.
InParanoidiP13286.
KOiK00871.
OMAiQNRYTAE.
OrthoDBiEOG7JMGDM.
PhylomeDBiP13286.
TreeFamiTF320349.

Family and domain databases

InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR002291. Phosph_kin_gamma.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 1 hit.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSiPR01049. PHOSPHBKNASE.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P13286-1 [UniParc]FASTAAdd to Basket

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MTRDEALPDS HSAQNFYENY EPKEILGRGV SSVVRRCIHK PTCQEYAVKI    50
IDITGGGSFS SEEVQELREA TLKEVDILQK VSGHPNIIQL KDTYETNTFF 100
FLVFDLMKRG ELFDYLTEKV TLTEKETRKI MRALLEVVCT LHKLNIVHRD 150
LKPENILLDD NMNIKLTDFG FSCQLQPGEK LREVCGTPSY LAPEIIQCSM 200
DEGHPGYGKE VDMWSTGVIM YTLLAGSPPF WHRKQMLMLR MIMDGKYQFG 250
SPEWDDYSDT VKDLVSRFLV VQPQDRCSAE EALAHPFFQE YVVEEVRHFS 300
PRGKFKVICL TVLASVRIYY QYRRVKPVTR EIVIRDPYAL RPLRRLIDAY 350
AFRIYGHWVK KGQQQNRAAL FENTPKAVLL SLAEEEDF 388
Length:388
Mass (Da):45,015
Last modified:January 23, 2007 - v2
Checksum:i6ABA8A51D527C149
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X07320 mRNA. Translation: CAA30280.1.
PIRiS00731.
RefSeqiNP_113761.1. NM_031573.1.
UniGeneiRn.10399.

Genome annotation databases

EnsembliENSRNOT00000001222; ENSRNOP00000001222; ENSRNOG00000000920.
GeneIDi29353.
KEGGirno:29353.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X07320 mRNA. Translation: CAA30280.1 .
PIRi S00731.
RefSeqi NP_113761.1. NM_031573.1.
UniGenei Rn.10399.

3D structure databases

ProteinModelPortali P13286.
SMRi P13286. Positions 12-292.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 248008. 1 interaction.
STRINGi 10116.ENSRNOP00000001222.

Proteomic databases

PaxDbi P13286.
PRIDEi P13286.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000001222 ; ENSRNOP00000001222 ; ENSRNOG00000000920 .
GeneIDi 29353.
KEGGi rno:29353.

Organism-specific databases

CTDi 5260.
RGDi 3325. Phkg1.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00750000117716.
HOGENOMi HOG000233016.
HOVERGENi HBG106193.
InParanoidi P13286.
KOi K00871.
OMAi QNRYTAE.
OrthoDBi EOG7JMGDM.
PhylomeDBi P13286.
TreeFami TF320349.

Enzyme and pathway databases

BRENDAi 2.7.11.19. 5301.
Reactomei REACT_227999. Glycogen breakdown (glycogenolysis).

Miscellaneous databases

NextBioi 608858.
PROi P13286.

Gene expression databases

Genevestigatori P13286.

Family and domain databases

InterProi IPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR002291. Phosph_kin_gamma.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
PANTHERi PTHR24347. PTHR24347. 1 hit.
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
PRINTSi PR01049. PHOSPHBKNASE.
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence of cDNA encoding the catalytic subunit of phosphorylase kinase from rat soleus muscle."
    Cawley K.C., Ramachandran C., Gorin F.A., Walsh D.A.
    Nucleic Acids Res. 16:2355-2356(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Characterization of the gene for rat phosphorylase kinase catalytic subunit."
    Cawley K.C., Akita C.G., Angelos K.L., Walsh D.A.
    J. Biol. Chem. 268:1194-1200(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.

Entry informationi

Entry nameiPHKG1_RAT
AccessioniPrimary (citable) accession number: P13286
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 23, 2007
Last modified: September 3, 2014
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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