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Protein

Phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoform

Gene

Phkg1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic subunit of the phosphorylase b kinase (PHK), which mediates the neural and hormonal regulation of glycogen breakdown (glycogenolysis) by phosphorylating and thereby activating glycogen phosphorylase. In vitro, phosphorylates PYGM, TNNI3, MAPT/TAU, GAP43 and NRGN/RC3 (By similarity).By similarity

Catalytic activityi

2 ATP + phosphorylase b = 2 ADP + phosphorylase a.
ATP + [tau protein] = ADP + [tau protein] phosphate.
ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei49 – 491ATPPROSITE-ProRule annotation
Active sitei150 – 1501Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi26 – 349ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: RGD
  • enzyme binding Source: RGD
  • phosphorylase kinase activity Source: RGD
  • tau-protein kinase activity Source: UniProtKB-EC

GO - Biological processi

  • glycogen biosynthetic process Source: InterPro
  • glycogen metabolic process Source: RGD
  • protein phosphorylation Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Muscle protein, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen metabolism

Keywords - Ligandi

ATP-binding, Calmodulin-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.19. 5301.
ReactomeiREACT_303296. Glycogen breakdown (glycogenolysis).

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoform (EC:2.7.11.19)
Alternative name(s):
Phosphorylase kinase subunit gamma-1
Serine/threonine-protein kinase PHKG1 (EC:2.7.11.1, EC:2.7.11.26)
Gene namesi
Name:Phkg1
Synonyms:Phkg
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 12

Organism-specific databases

RGDi3325. Phkg1.

Subcellular locationi

GO - Cellular componenti

  • phosphorylase kinase complex Source: RGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 388387Phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoformPRO_0000086511Add
BLAST

Proteomic databases

PaxDbiP13286.
PRIDEiP13286.

Expressioni

Gene expression databases

ExpressionAtlasiP13286. baseline and differential.
GenevisibleiP13286. RN.

Interactioni

Subunit structurei

Hexadecamer of 4 heterotetramers, each composed of alpha, beta, gamma, and delta subunits. Alpha (PHKA1 or PHKA2) and beta (PHKB) are regulatory subunits, gamma (PHKG1 or PHKG2) is the catalytic subunit, and delta is calmodulin.

Protein-protein interaction databases

BioGridi248008. 1 interaction.
STRINGi10116.ENSRNOP00000001222.

Structurei

3D structure databases

ProteinModelPortaliP13286.
SMRiP13286. Positions 12-292.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini20 – 288269Protein kinasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni303 – 32725Calmodulin-binding (domain-N)Add
BLAST
Regioni343 – 36725Calmodulin-binding (domain-C)Add
BLAST

Domaini

The two calmodulin-binding domains appear to act in concert to bind a single molecule of calmodulin and are pseudosubstrate/autoinhibitory domains.By similarity

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00780000121878.
HOGENOMiHOG000233016.
HOVERGENiHBG106193.
InParanoidiP13286.
KOiK00871.
OMAiSAQGFYE.
OrthoDBiEOG7JMGDM.
PhylomeDBiP13286.
TreeFamiTF320349.

Family and domain databases

InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR002291. Phosph_kin_gamma.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 1 hit.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSiPR01049. PHOSPHBKNASE.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P13286-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTRDEALPDS HSAQNFYENY EPKEILGRGV SSVVRRCIHK PTCQEYAVKI
60 70 80 90 100
IDITGGGSFS SEEVQELREA TLKEVDILQK VSGHPNIIQL KDTYETNTFF
110 120 130 140 150
FLVFDLMKRG ELFDYLTEKV TLTEKETRKI MRALLEVVCT LHKLNIVHRD
160 170 180 190 200
LKPENILLDD NMNIKLTDFG FSCQLQPGEK LREVCGTPSY LAPEIIQCSM
210 220 230 240 250
DEGHPGYGKE VDMWSTGVIM YTLLAGSPPF WHRKQMLMLR MIMDGKYQFG
260 270 280 290 300
SPEWDDYSDT VKDLVSRFLV VQPQDRCSAE EALAHPFFQE YVVEEVRHFS
310 320 330 340 350
PRGKFKVICL TVLASVRIYY QYRRVKPVTR EIVIRDPYAL RPLRRLIDAY
360 370 380
AFRIYGHWVK KGQQQNRAAL FENTPKAVLL SLAEEEDF
Length:388
Mass (Da):45,015
Last modified:January 23, 2007 - v2
Checksum:i6ABA8A51D527C149
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07320 mRNA. Translation: CAA30280.1.
PIRiS00731.
RefSeqiNP_113761.1. NM_031573.1.
UniGeneiRn.10399.

Genome annotation databases

EnsembliENSRNOT00000001222; ENSRNOP00000001222; ENSRNOG00000000920.
GeneIDi29353.
KEGGirno:29353.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07320 mRNA. Translation: CAA30280.1.
PIRiS00731.
RefSeqiNP_113761.1. NM_031573.1.
UniGeneiRn.10399.

3D structure databases

ProteinModelPortaliP13286.
SMRiP13286. Positions 12-292.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248008. 1 interaction.
STRINGi10116.ENSRNOP00000001222.

Proteomic databases

PaxDbiP13286.
PRIDEiP13286.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000001222; ENSRNOP00000001222; ENSRNOG00000000920.
GeneIDi29353.
KEGGirno:29353.

Organism-specific databases

CTDi5260.
RGDi3325. Phkg1.

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00780000121878.
HOGENOMiHOG000233016.
HOVERGENiHBG106193.
InParanoidiP13286.
KOiK00871.
OMAiSAQGFYE.
OrthoDBiEOG7JMGDM.
PhylomeDBiP13286.
TreeFamiTF320349.

Enzyme and pathway databases

BRENDAi2.7.11.19. 5301.
ReactomeiREACT_303296. Glycogen breakdown (glycogenolysis).

Miscellaneous databases

NextBioi608858.
PROiP13286.

Gene expression databases

ExpressionAtlasiP13286. baseline and differential.
GenevisibleiP13286. RN.

Family and domain databases

InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR002291. Phosph_kin_gamma.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 1 hit.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSiPR01049. PHOSPHBKNASE.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Nucleotide sequence of cDNA encoding the catalytic subunit of phosphorylase kinase from rat soleus muscle."
    Cawley K.C., Ramachandran C., Gorin F.A., Walsh D.A.
    Nucleic Acids Res. 16:2355-2356(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Characterization of the gene for rat phosphorylase kinase catalytic subunit."
    Cawley K.C., Akita C.G., Angelos K.L., Walsh D.A.
    J. Biol. Chem. 268:1194-1200(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.

Entry informationi

Entry nameiPHKG1_RAT
AccessioniPrimary (citable) accession number: P13286
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 23, 2007
Last modified: July 22, 2015
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.