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P13286

- PHKG1_RAT

UniProt

P13286 - PHKG1_RAT

Protein

Phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoform

Gene

Phkg1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalytic subunit of the phosphorylase b kinase (PHK), which mediates the neural and hormonal regulation of glycogen breakdown (glycogenolysis) by phosphorylating and thereby activating glycogen phosphorylase. In vitro, phosphorylates PYGM, TNNI3, MAPT/TAU, GAP43 and NRGN/RC3 By similarity.By similarity

    Catalytic activityi

    2 ATP + phosphorylase b = 2 ADP + phosphorylase a.
    ATP + [tau protein] = ADP + [tau protein] phosphate.
    ATP + a protein = ADP + a phosphoprotein.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei49 – 491ATPPROSITE-ProRule annotation
    Active sitei150 – 1501Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi26 – 349ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: RGD
    2. enzyme binding Source: RGD
    3. phosphorylase kinase activity Source: RGD
    4. tau-protein kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glycogen biosynthetic process Source: InterPro
    2. glycogen metabolic process Source: RGD
    3. protein phosphorylation Source: RGD

    Keywords - Molecular functioni

    Kinase, Muscle protein, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Carbohydrate metabolism, Glycogen metabolism

    Keywords - Ligandi

    ATP-binding, Calmodulin-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.11.19. 5301.
    ReactomeiREACT_227999. Glycogen breakdown (glycogenolysis).

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoform (EC:2.7.11.19)
    Alternative name(s):
    Phosphorylase kinase subunit gamma-1
    Serine/threonine-protein kinase PHKG1 (EC:2.7.11.1, EC:2.7.11.26)
    Gene namesi
    Name:Phkg1
    Synonyms:Phkg
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 12

    Organism-specific databases

    RGDi3325. Phkg1.

    Subcellular locationi

    GO - Cellular componenti

    1. phosphorylase kinase complex Source: RGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 388387Phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoformPRO_0000086511Add
    BLAST

    Proteomic databases

    PaxDbiP13286.
    PRIDEiP13286.

    Expressioni

    Gene expression databases

    GenevestigatoriP13286.

    Interactioni

    Subunit structurei

    Hexadecamer of 4 heterotetramers, each composed of alpha, beta, gamma, and delta subunits. Alpha (PHKA1 or PHKA2) and beta (PHKB) are regulatory subunits, gamma (PHKG1 or PHKG2) is the catalytic subunit, and delta is calmodulin.

    Protein-protein interaction databases

    BioGridi248008. 1 interaction.
    STRINGi10116.ENSRNOP00000001222.

    Structurei

    3D structure databases

    ProteinModelPortaliP13286.
    SMRiP13286. Positions 12-292.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini20 – 288269Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni303 – 32725Calmodulin-binding (domain-N)Add
    BLAST
    Regioni343 – 36725Calmodulin-binding (domain-C)Add
    BLAST

    Domaini

    The two calmodulin-binding domains appear to act in concert to bind a single molecule of calmodulin and are pseudosubstrate/autoinhibitory domains.By similarity

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00750000117716.
    HOGENOMiHOG000233016.
    HOVERGENiHBG106193.
    InParanoidiP13286.
    KOiK00871.
    OMAiQNRYTAE.
    OrthoDBiEOG7JMGDM.
    PhylomeDBiP13286.
    TreeFamiTF320349.

    Family and domain databases

    InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
    IPR011009. Kinase-like_dom.
    IPR002291. Phosph_kin_gamma.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PANTHERiPTHR24347. PTHR24347. 1 hit.
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    PRINTSiPR01049. PHOSPHBKNASE.
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P13286-1 [UniParc]FASTAAdd to Basket

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    MTRDEALPDS HSAQNFYENY EPKEILGRGV SSVVRRCIHK PTCQEYAVKI    50
    IDITGGGSFS SEEVQELREA TLKEVDILQK VSGHPNIIQL KDTYETNTFF 100
    FLVFDLMKRG ELFDYLTEKV TLTEKETRKI MRALLEVVCT LHKLNIVHRD 150
    LKPENILLDD NMNIKLTDFG FSCQLQPGEK LREVCGTPSY LAPEIIQCSM 200
    DEGHPGYGKE VDMWSTGVIM YTLLAGSPPF WHRKQMLMLR MIMDGKYQFG 250
    SPEWDDYSDT VKDLVSRFLV VQPQDRCSAE EALAHPFFQE YVVEEVRHFS 300
    PRGKFKVICL TVLASVRIYY QYRRVKPVTR EIVIRDPYAL RPLRRLIDAY 350
    AFRIYGHWVK KGQQQNRAAL FENTPKAVLL SLAEEEDF 388
    Length:388
    Mass (Da):45,015
    Last modified:January 23, 2007 - v2
    Checksum:i6ABA8A51D527C149
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X07320 mRNA. Translation: CAA30280.1.
    PIRiS00731.
    RefSeqiNP_113761.1. NM_031573.1.
    UniGeneiRn.10399.

    Genome annotation databases

    EnsembliENSRNOT00000001222; ENSRNOP00000001222; ENSRNOG00000000920.
    GeneIDi29353.
    KEGGirno:29353.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X07320 mRNA. Translation: CAA30280.1 .
    PIRi S00731.
    RefSeqi NP_113761.1. NM_031573.1.
    UniGenei Rn.10399.

    3D structure databases

    ProteinModelPortali P13286.
    SMRi P13286. Positions 12-292.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 248008. 1 interaction.
    STRINGi 10116.ENSRNOP00000001222.

    Proteomic databases

    PaxDbi P13286.
    PRIDEi P13286.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000001222 ; ENSRNOP00000001222 ; ENSRNOG00000000920 .
    GeneIDi 29353.
    KEGGi rno:29353.

    Organism-specific databases

    CTDi 5260.
    RGDi 3325. Phkg1.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00750000117716.
    HOGENOMi HOG000233016.
    HOVERGENi HBG106193.
    InParanoidi P13286.
    KOi K00871.
    OMAi QNRYTAE.
    OrthoDBi EOG7JMGDM.
    PhylomeDBi P13286.
    TreeFami TF320349.

    Enzyme and pathway databases

    BRENDAi 2.7.11.19. 5301.
    Reactomei REACT_227999. Glycogen breakdown (glycogenolysis).

    Miscellaneous databases

    NextBioi 608858.
    PROi P13286.

    Gene expression databases

    Genevestigatori P13286.

    Family and domain databases

    InterProi IPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
    IPR011009. Kinase-like_dom.
    IPR002291. Phosph_kin_gamma.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    PANTHERi PTHR24347. PTHR24347. 1 hit.
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    PRINTSi PR01049. PHOSPHBKNASE.
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of cDNA encoding the catalytic subunit of phosphorylase kinase from rat soleus muscle."
      Cawley K.C., Ramachandran C., Gorin F.A., Walsh D.A.
      Nucleic Acids Res. 16:2355-2356(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Characterization of the gene for rat phosphorylase kinase catalytic subunit."
      Cawley K.C., Akita C.G., Angelos K.L., Walsh D.A.
      J. Biol. Chem. 268:1194-1200(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Wistar.

    Entry informationi

    Entry nameiPHKG1_RAT
    AccessioniPrimary (citable) accession number: P13286
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 123 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3