Phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoform

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P13286 (PHKG1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 114. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoform
EC=2.7.11.19

Alternative name(s):
Phosphorylase kinase subunit gamma-1
Serine/threonine-protein kinase PHKG1
EC=2.7.11.1
EC=2.7.11.26

Gene names

Name:	Phkg1
Synonyms:	Phkg

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	388 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalytic subunit of the phosphorylase b kinase (PHK), which mediates the neural and hormonal regulation of glycogen breakdown (glycogenolysis) by phosphorylating and thereby activating glycogen phosphorylase. In vitro, phosphorylates PYGM, TNNI3, MAPT/TAU, GAP43 and NRGN/RC3 By similarity.
Catalytic activity	2 ATP + phosphorylase b = 2 ADP + phosphorylase a. ATP + [tau protein] = ADP + [tau protein] phosphate. ATP + a protein = ADP + a phosphoprotein.
Subunit structure	Hexadecamer of 4 heterotetramers, each composed of alpha, beta, gamma, and delta subunits. Alpha (PHKA1 or PHKA2) and beta (PHKB) are regulatory subunits, gamma (PHKG1 or PHKG2) is the catalytic subunit, and delta is calmodulin.
Domain	The two calmodulin-binding domains appear to act in concert to bind a single molecule of calmodulin and are pseudosubstrate/autoinhibitory domains By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glycogen metabolism
Ligand	ATP-binding Calmodulin-binding Nucleotide-binding
Molecular function	Kinase Muscle protein Serine/threonine-protein kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glycogen biosynthetic process Inferred from electronic annotation. Source: InterPro glycogen metabolic process Inferred from direct assay PubMed 3953807 PubMed 6281247. Source: RGD
Cellular_component	phosphorylase kinase complex Inferred from direct assay PubMed 3953807. Source: RGD
Molecular_function	ATP binding Inferred from direct assay PubMed 6281247. Source: RGD enzyme binding Inferred from direct assay PubMed 3953807 PubMed 6281247. Source: RGD phosphorylase kinase activity Inferred from direct assay PubMed 3953807 PubMed 6281247. Source: RGD tau-protein kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed

Chain

2 – 388

387

Phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoform

PRO_0000086511

Regions

Domain

20 – 288

269

Protein kinase

Nucleotide binding

26 – 34

ATP By similarity

Region

303 – 327

Calmodulin-binding (domain-N)

Region

343 – 367

Calmodulin-binding (domain-C)

Sites

Active site

150

Proton acceptor By similarity

Binding site

ATP By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

P13286 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 6ABA8A51D527C149
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FASTA

388

45,015

        10         20         30         40         50         60 
MTRDEALPDS HSAQNFYENY EPKEILGRGV SSVVRRCIHK PTCQEYAVKI IDITGGGSFS 

        70         80         90        100        110        120 
SEEVQELREA TLKEVDILQK VSGHPNIIQL KDTYETNTFF FLVFDLMKRG ELFDYLTEKV 

       130        140        150        160        170        180 
TLTEKETRKI MRALLEVVCT LHKLNIVHRD LKPENILLDD NMNIKLTDFG FSCQLQPGEK 

       190        200        210        220        230        240 
LREVCGTPSY LAPEIIQCSM DEGHPGYGKE VDMWSTGVIM YTLLAGSPPF WHRKQMLMLR 

       250        260        270        280        290        300 
MIMDGKYQFG SPEWDDYSDT VKDLVSRFLV VQPQDRCSAE EALAHPFFQE YVVEEVRHFS 

       310        320        330        340        350        360 
PRGKFKVICL TVLASVRIYY QYRRVKPVTR EIVIRDPYAL RPLRRLIDAY AFRIYGHWVK 

       370        380 
KGQQQNRAAL FENTPKAVLL SLAEEEDF

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References

[1]	"Nucleotide sequence of cDNA encoding the catalytic subunit of phosphorylase kinase from rat soleus muscle." Cawley K.C., Ramachandran C., Gorin F.A., Walsh D.A. Nucleic Acids Res. 16:2355-2356(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Characterization of the gene for rat phosphorylase kinase catalytic subunit." Cawley K.C., Akita C.G., Angelos K.L., Walsh D.A. J. Biol. Chem. 268:1194-1200(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X07320 mRNA. Translation: CAA30280.1.
IPI	IPI00231199.
PIR	S00731.
RefSeq	NP_113761.1. NM_031573.1.
UniGene	Rn.10399.
3D structure databases
ProteinModelPortal	P13286.
SMR	P13286. Positions 12-292.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000001222.
Proteomic databases
PaxDb	P13286.
PRIDE	P13286.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000001222; ENSRNOP00000001222; ENSRNOG00000000920.
GeneID	29353.
KEGG	rno:29353.
Organism-specific databases
CTD	5260.
RGD	3325. Phkg1.
Phylogenomic databases
eggNOG	COG0515.
GeneTree	ENSGT00680000100008.
HOGENOM	HOG000233016.
HOVERGEN	HBG106193.
InParanoid	P13286.
KO	K00871.
OMA	DFYENYE.
OrthoDB	EOG4CNQR5.
Enzyme and pathway databases
BRENDA	2.7.11.19. 5301.
Gene expression databases
ArrayExpress	P13286.
Genevestigator	P13286.
GermOnline	ENSRNOG00000000920. Rattus norvegicus.
Family and domain databases
InterPro	IPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase. IPR011009. Kinase-like_dom. IPR002291. Phosph_kin_gamma. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
PANTHER	PTHR24347. PTHR24347. 1 hit.
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
PRINTS	PR01049. PHOSPHBKNASE.
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	608858.

Entry information

Entry name

PHKG1_RAT

Accession

Primary (citable) accession number: P13286

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 114 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents