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Protein

Gamma-interferon-inducible lysosomal thiol reductase

Gene

IFI30

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Lysosomal thiol reductase that can reduce protein disulfide bonds. May facilitate the complete unfolding of proteins destined for lysosomal degradation. Plays an important role in antigen processing. Facilitates the generation of MHC class II-restricted epitodes from disulfide bond-containing antigen by the endocytic reduction of disulfide bonds (By similarity). Facilitates also MHC class I-restricted recognition of exogenous antigens containing disulfide bonds by CD8+ T-cells or crosspresentation (By similarity).By similarity

Kineticsi

Kinetic parameters shown are for mature enzyme.

  1. KM=1200 µM for F(ab')2 fragment when denatured1 Publication
  2. KM=10 µM for F(ab')2 fragment1 Publication

    pH dependencei

    Optimum pH is 4-5.1 Publication

    GO - Molecular functioni

    • oxidoreductase activity, acting on a sulfur group of donors Source: UniProtKB

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Immunity

    Enzyme and pathway databases

    BioCyciZFISH:G66-33206-MONOMER.
    ReactomeiR-HSA-2132295. MHC class II antigen presentation.
    R-HSA-877300. Interferon gamma signaling.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Gamma-interferon-inducible lysosomal thiol reductase (EC:1.8.-.-)
    Alternative name(s):
    Gamma-interferon-inducible protein IP-30
    Legumaturain
    Gene namesi
    Name:IFI30
    Synonyms:GILT, IP30
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    Proteomesi
    • UP000005640 Componenti: Chromosome 19

    Organism-specific databases

    HGNCiHGNC:5398. IFI30.

    Subcellular locationi

    GO - Cellular componenti

    • cell junction Source: HPA
    • cytoplasm Source: HPA
    • extracellular region Source: ProtInc
    • intracellular membrane-bounded organelle Source: HPA
    • lysosomal lumen Source: Reactome
    • lysosome Source: UniProtKB
    • plasma membrane Source: HPA
    Complete GO annotation...

    Keywords - Cellular componenti

    Lysosome, Secreted

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi72C → S: Abolishes reducing activity, does not affect dimerization. Abolishes reducing activity; when associated with S-75. 2 Publications1
    Mutagenesisi75C → S: Abolishes reducing activity, does not affect dimerization. Abolishes reducing activity; when associated with S-72. 2 Publications1

    Organism-specific databases

    DisGeNETi10437.
    OpenTargetsiENSG00000216490.
    PharmGKBiPA29644.

    Polymorphism and mutation databases

    BioMutaiIFI30.
    DMDMi327478582.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Signal peptidei1 – 26Sequence analysisAdd BLAST26
    PropeptideiPRO_000002132827 – 57Removed in mature form1 PublicationAdd BLAST31
    ChainiPRO_000002132958 – 232Gamma-interferon-inducible lysosomal thiol reductaseAdd BLAST175
    PropeptideiPRO_0000021330233 – 250Removed in mature form1 PublicationAdd BLAST18

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Glycosylationi63N-linked (GlcNAc...)Sequence analysis1
    Disulfide bondi72 ↔ 75Redox-active
    Glycosylationi95N-linked (GlcNAc...)Sequence analysis1
    Glycosylationi108N-linked (GlcNAc...)Sequence analysis1

    Post-translational modificationi

    N-glycosylated. Sugar chains contain mannose-6-phosphate.2 Publications
    Synthesized as a 35 kDa precursor which is then processed into the mature 30 kDa form via cleavage of N-terminal and C-terminal propeptides. Processing of the precursor is mediated by multiple lysosomal proteases.

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    EPDiP13284.
    MaxQBiP13284.
    PaxDbiP13284.
    PeptideAtlasiP13284.
    PRIDEiP13284.

    PTM databases

    iPTMnetiP13284.
    PhosphoSitePlusiP13284.

    Expressioni

    Inductioni

    Expressed constitutively in antigen-presenting cells and induced by IFN-gamma in other cell types.1 Publication

    Gene expression databases

    BgeeiENSG00000216490.
    CleanExiHS_IFI30.
    ExpressionAtlasiP13284. baseline and differential.
    GenevisibleiP13284. HS.

    Organism-specific databases

    HPAiHPA026650.

    Interactioni

    Subunit structurei

    Dimer; disulfide-linked.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    KRTAP4-12Q9BQ663EBI-2868897,EBI-739863
    NOTCH2NLQ7Z3S93EBI-2868897,EBI-945833

    Protein-protein interaction databases

    BioGridi115704. 41 interactors.
    IntActiP13284. 8 interactors.
    STRINGi9606.ENSP00000384886.

    Structurei

    3D structure databases

    ProteinModelPortaliP13284.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the GILT family.Curated

    Keywords - Domaini

    Redox-active center, Signal

    Phylogenomic databases

    eggNOGiKOG3160. Eukaryota.
    ENOG4111IDT. LUCA.
    GeneTreeiENSGT00390000010450.
    HOGENOMiHOG000238423.
    HOVERGENiHBG005837.
    InParanoidiP13284.
    KOiK08059.
    OMAiMHANAQR.
    OrthoDBiEOG091G0IMC.
    PhylomeDBiP13284.
    TreeFamiTF315141.

    Family and domain databases

    InterProiIPR004911. Interferon-induced_GILT.
    [Graphical view]
    PANTHERiPTHR13234. PTHR13234. 1 hit.
    PfamiPF03227. GILT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P13284-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MTLSPLLLFL PPLLLLLDVP TAAVQASPLQ ALDFFGNGPP VNYKTGNLYL
    60 70 80 90 100
    RGPLKKSNAP LVNVTLYYEA LCGGCRAFLI RELFPTWLLV MEILNVTLVP
    110 120 130 140 150
    YGNAQEQNVS GRWEFKCQHG EEECKFNKVE ACVLDELDME LAFLTIVCME
    160 170 180 190 200
    EFEDMERSLP LCLQLYAPGL SPDTIMECAM GDRGMQLMHA NAQRTDALQP
    210 220 230 240 250
    PHEYVPWVTV NGKPLEDQTQ LLTLVCQLYQ GKKPDVCPSS TSSLRSVCFK
    Length:250
    Mass (Da):27,964
    Last modified:April 5, 2011 - v3
    Checksum:i54B4950E3788CD5A
    GO

    Sequence cautioni

    The sequence AAA36105 differs from that shown. Chimeric cDNA. N-terminal sequence identical to a region of chromosome 11.Curated
    The sequence AAA36105 differs from that shown. Reason: Frameshift at positions 146 and 174.Curated
    The sequence AAA36105 differs from that shown. Reason: Erroneous termination at position 251. Translated as stop.Curated
    The sequence AAF04618 differs from that shown. Chimeric cDNA. N-terminal sequence identical to a region of chromosome 11.Curated

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti76R → Q in AAH31020 (PubMed:15489334).Curated1
    Sequence conflicti98L → S in AAA36105 (PubMed:3136170).Curated1
    Sequence conflicti119H → L in AAA36105 (PubMed:3136170).Curated1
    Sequence conflicti148C → WHG in AAA36105 (PubMed:3136170).Curated1
    Sequence conflicti223T → A in BAC98466 (Ref. 3) Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    J03909 mRNA. Translation: AAA36105.1. Sequence problems.
    AF097362 mRNA. Translation: AAF04618.1. Sequence problems.
    AB049659 mRNA. Translation: BAC98466.1.
    AC007192 Genomic DNA. No translation available.
    AC093080 Genomic DNA. No translation available.
    AC068499 Genomic DNA. No translation available.
    CH471106 Genomic DNA. Translation: EAW84662.1.
    BC021136 mRNA. Translation: AAH21136.1.
    BC031020 mRNA. Translation: AAH31020.1.
    CCDSiCCDS46015.1.
    PIRiA43708.
    RefSeqiNP_006323.2. NM_006332.4.
    UniGeneiHs.14623.

    Genome annotation databases

    EnsembliENST00000407280; ENSP00000384886; ENSG00000216490.
    GeneIDi10437.
    KEGGihsa:10437.
    UCSCiuc002nic.3. human.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    J03909 mRNA. Translation: AAA36105.1. Sequence problems.
    AF097362 mRNA. Translation: AAF04618.1. Sequence problems.
    AB049659 mRNA. Translation: BAC98466.1.
    AC007192 Genomic DNA. No translation available.
    AC093080 Genomic DNA. No translation available.
    AC068499 Genomic DNA. No translation available.
    CH471106 Genomic DNA. Translation: EAW84662.1.
    BC021136 mRNA. Translation: AAH21136.1.
    BC031020 mRNA. Translation: AAH31020.1.
    CCDSiCCDS46015.1.
    PIRiA43708.
    RefSeqiNP_006323.2. NM_006332.4.
    UniGeneiHs.14623.

    3D structure databases

    ProteinModelPortaliP13284.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi115704. 41 interactors.
    IntActiP13284. 8 interactors.
    STRINGi9606.ENSP00000384886.

    PTM databases

    iPTMnetiP13284.
    PhosphoSitePlusiP13284.

    Polymorphism and mutation databases

    BioMutaiIFI30.
    DMDMi327478582.

    Proteomic databases

    EPDiP13284.
    MaxQBiP13284.
    PaxDbiP13284.
    PeptideAtlasiP13284.
    PRIDEiP13284.

    Protocols and materials databases

    DNASUi10437.
    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENST00000407280; ENSP00000384886; ENSG00000216490.
    GeneIDi10437.
    KEGGihsa:10437.
    UCSCiuc002nic.3. human.

    Organism-specific databases

    CTDi10437.
    DisGeNETi10437.
    GeneCardsiIFI30.
    HGNCiHGNC:5398. IFI30.
    HPAiHPA026650.
    MIMi604664. gene.
    neXtProtiNX_P13284.
    OpenTargetsiENSG00000216490.
    PharmGKBiPA29644.
    GenAtlasiSearch...

    Phylogenomic databases

    eggNOGiKOG3160. Eukaryota.
    ENOG4111IDT. LUCA.
    GeneTreeiENSGT00390000010450.
    HOGENOMiHOG000238423.
    HOVERGENiHBG005837.
    InParanoidiP13284.
    KOiK08059.
    OMAiMHANAQR.
    OrthoDBiEOG091G0IMC.
    PhylomeDBiP13284.
    TreeFamiTF315141.

    Enzyme and pathway databases

    BioCyciZFISH:G66-33206-MONOMER.
    ReactomeiR-HSA-2132295. MHC class II antigen presentation.
    R-HSA-877300. Interferon gamma signaling.

    Miscellaneous databases

    GeneWikiiIFI30.
    GenomeRNAii10437.
    PROiP13284.
    SOURCEiSearch...

    Gene expression databases

    BgeeiENSG00000216490.
    CleanExiHS_IFI30.
    ExpressionAtlasiP13284. baseline and differential.
    GenevisibleiP13284. HS.

    Family and domain databases

    InterProiIPR004911. Interferon-induced_GILT.
    [Graphical view]
    PANTHERiPTHR13234. PTHR13234. 1 hit.
    PfamiPF03227. GILT. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiGILT_HUMAN
    AccessioniPrimary (citable) accession number: P13284
    Secondary accession number(s): Q76MF9
    , Q8NEI4, Q8WU77, Q9UL08
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: April 5, 2011
    Last modified: November 30, 2016
    This is version 129 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Miscellaneous

    Both precursor form and mature form have thiol reductase activity.

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 19
      Human chromosome 19: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.